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<document ID-DOI="http://doi.org/10.5281/zenodo.6010900" ID-GBIF-Dataset="ea0932c3-01e6-4c74-90a2-4d1999acbdaf" ID-GBIF-Taxon="132923494" ID-Zenodo-Dep="6010900" checkinTime="1495093354150" checkinUser="plazi" docAuthor="Tomáš Scholz, Alain de Chambrier, Takeshi Shimazu, Alexey Ermolenko &amp; Andrea Waeschenbach" docDate="2017" docId="632E7F4C6F7AFF91FFF80584FD60FD70" docLanguage="en" docName="Scholz_etal_2017.pdf" docOrigin="Parasitology International 66" docStyle="DocumentStyle{}" docTitle="Proteocephalus misgurni Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach 2017, sp. n." docType="treatment" docVersion="5" lastPageId="10" lastPageNumber="881" masterDocId="9F1707346F7DFF9BFFA20368FFB1FFAD" masterDocTitle="Proteocephalid tapeworms (Cestoda: Onchoproteocephalidea) of loaches (Cobitoidea): Evidence for monophyly and high endemism of parasites in the Far East" masterLastPageNumber="883" masterPageNumber="871" pageId="7" pageNumber="878" updateTime="1644331272124" updateUser="ExternalLinkService">
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<mods:titleInfo>
<mods:title>Proteocephalid tapeworms (Cestoda: Onchoproteocephalidea) of loaches (Cobitoidea): Evidence for monophyly and high endemism of parasites in the Far East</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tomáš Scholz</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Alain de Chambrier</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Takeshi Shimazu</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Alexey Ermolenko</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Andrea Waeschenbach</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
<mods:title>Parasitology International</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2017</mods:date>
<mods:detail type="volume">
<mods:number>66</mods:number>
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<mods:start>871</mods:start>
<mods:end>883</mods:end>
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<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.1016/j.parint.2016.09.0161383-5769</mods:identifier>
<mods:identifier type="GBIF-Dataset">ea0932c3-01e6-4c74-90a2-4d1999acbdaf</mods:identifier>
<mods:identifier type="Zenodo-Dep">886409</mods:identifier>
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<subSection lastPageId="8" lastPageNumber="879" pageId="7" pageNumber="878" type="multiple">
<treatment ID-DOI="http://doi.org/10.5281/zenodo.6010900" ID-GBIF-Taxon="132923494" ID-Zenodo-Dep="6010900" LSID="urn:lsid:plazi:treatment:632E7F4C6F7AFF91FFF80584FD60FD70" httpUri="http://treatment.plazi.org/id/632E7F4C6F7AFF91FFF80584FD60FD70" lastPageId="10" lastPageNumber="881" pageId="7" pageNumber="878">
<heading box="[90,506,1772,1793]" fontSize="8" level="2" pageId="7" pageNumber="878" reason="1">
<subSubSection box="[90,424,1772,1793]" pageId="7" pageNumber="878" type="nomenclature">
<paragraph blockId="7.[90,760,1772,1988]" box="[90,424,1772,1793]" pageId="7" pageNumber="878">
<emphasis box="[90,366,1772,1792]" italics="true" pageId="7" pageNumber="878">
3.2.4.
<taxonomicName authority="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach, 2017" authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[143,366,1772,1792]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="7" pageNumber="878" phylum="Platyhelminthes" rank="species" species="misgurni" status="sp. n.">
<taxonomicName box="[143,280,1772,1792]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" higherTaxonomySource="CoL" kingdom="Animalia" order="Onchoproteocephalidea" pageId="7" pageNumber="878" phylum="Platyhelminthes" rank="genus">Proteocephalus</taxonomicName>
<taxonomicName box="[286,366,1772,1792]" pageId="7" pageNumber="878" rank="species" species="misgurni" status="sp. n.">misgurni</taxonomicName>
</taxonomicName>
</emphasis>
<emphasis bold="true" box="[371,424,1774,1793]" pageId="7" pageNumber="878">
<taxonomicNameLabel box="[371,424,1774,1793]" pageId="7" pageNumber="878" rank="species">sp. n.</taxonomicNameLabel>
</emphasis>
</paragraph>
</subSubSection>
<subSubSection lastPageId="9" lastPageNumber="880" pageId="7" pageNumber="878" type="description">
<paragraph blockId="7.[90,760,1772,1988]" box="[428,506,1772,1792]" pageId="7" pageNumber="878">
<emphasis box="[428,506,1772,1792]" italics="true" pageId="7" pageNumber="878">
<figureCitation box="[428,486,1772,1792]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Figs. 5</figureCitation>
,
<figureCitation box="[495,506,1772,1792]" captionStart="Fig. 6" captionStartId="9.[90,115,1331,1345]" captionTargetBox="[234,1330,144,1294]" captionTargetId="figure@9.[232,1332,142,1295]" captionTargetPageId="9" captionText="Fig. 6. Scanning electron micrograph of Proteocephalus demshini n. sp. from Barbatula toni, Russia (A, C, D, F) and Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus, Russia (B, E, G, H). A, B — dorsoventral view, C — apical view, D — lateral view, E — subapical view, F — acicular filitriches and gladiate spinitriches on internal rim of suckers, G — acicular filitriches between suckers, H — gladiate spinitriches on neck region. Small letters in A and B indicate position of microtriches on F H)." httpUri="https://zenodo.org/record/886423/files/figure.png" pageId="7" pageNumber="878">6</figureCitation>
</emphasis>
</paragraph>
<paragraph blockId="7.[90,760,1772,1988]" lastBlockId="7.[805,1475,1243,1988]" pageId="7" pageNumber="878">
<emphasis box="[122,225,1800,1820]" italics="true" pageId="7" pageNumber="878">Description</emphasis>
(based on 11 whole-mounted specimens and 2 scoleces studied using SEM; for morphometrical data see
<tableCitation box="[662,742,1829,1848]" captionStart="Table 2" captionStartId="5.[90,133,157,171]" captionText="Table 2 Comparative measurements of species of Proteocephalus (Cestoda: Onchoproteocephalidea) from loaches (Cobitoidea) in the Palaearctic region. Measurements in micrometres unless oth- erwise stated." pageId="7" pageNumber="878">Table 2</tableCitation>
):
<taxonomicName authority=", Proteocephalinae. Testes" authorityName="Proteocephalinae. Testes" box="[90,517,1857,1877]" class="Cestoda" family="Proteocephalidae" kingdom="Animalia" order="Proteocephalidea" pageId="7" pageNumber="878" phylum="Platyhelminthes" rank="family">
<taxonomicName authority="Proteocephalinae." authorityName="Proteocephalinae." box="[90,448,1857,1876]" family="Proteocephalidae" pageId="7" pageNumber="878" rank="family">
<taxonomicName authority="Proteocephalinae" authorityName="Proteocephalinae" box="[90,444,1857,1876]" family="Proteocephalidae" pageId="7" pageNumber="878" rank="family">Proteocephalidae, Proteocephalinae</taxonomicName>
.
</taxonomicName>
Testes
</taxonomicName>
, vitelline follicles, ovary and uterus medullary. Medium-sized tapeworm, body
<quantity box="[618,711,1885,1904]" metricMagnitude="-2" metricUnit="m" metricValue="1.5" metricValueMax="2.1" metricValueMin="0.9" pageId="7" pageNumber="878" unit="mm" value="15.0" valueMax="21.0" valueMin="9.0">921 mm</quantity>
long (n = 5), maximum width up to 530 (n = 10). Strobila acraspedote, anapolytic, consisting of 5258 proglottids: 3339 immature, 2 mature, 312 pregravid; 36 gravid proglottids (
<figureCitation box="[473,523,1969,1988]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
A). Immature proglottids and mature proglottids wider than long to longer than wide. Pregravid proglottids rectangular to longer than wide, gravid proglottids longer than wide.
</paragraph>
<paragraph blockId="7.[805,1475,1243,1988]" pageId="7" pageNumber="878">
Scolex 250350 long (n = 4), wider than neck (
<figureCitation box="[1323,1385,1327,1346]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Figs. 5</figureCitation>
C, 6B, E). Suckers spherical, with deep cavity (
<figureCitation box="[1172,1225,1355,1374]" captionStart="Fig. 6" captionStartId="9.[90,115,1331,1345]" captionTargetBox="[234,1330,144,1294]" captionTargetId="figure@9.[232,1332,142,1295]" captionTargetPageId="9" captionText="Fig. 6. Scanning electron micrograph of Proteocephalus demshini n. sp. from Barbatula toni, Russia (A, C, D, F) and Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus, Russia (B, E, G, H). A, B — dorsoventral view, C — apical view, D — lateral view, E — subapical view, F — acicular filitriches and gladiate spinitriches on internal rim of suckers, G — acicular filitriches between suckers, H — gladiate spinitriches on neck region. Small letters in A and B indicate position of microtriches on F H)." httpUri="https://zenodo.org/record/886423/files/figure.png" pageId="7" pageNumber="878">Fig. 6</figureCitation>
B, E). Neck (proliferation zone) widened, 340470 wide. Scolex covered with acicular filitriches and gladiate spinitriches (on suckers) and acicular filitriches (between suckers —
<figureCitation box="[910,963,1439,1458]" captionStart="Fig. 6" captionStartId="9.[90,115,1331,1345]" captionTargetBox="[234,1330,144,1294]" captionTargetId="figure@9.[232,1332,142,1295]" captionTargetPageId="9" captionText="Fig. 6. Scanning electron micrograph of Proteocephalus demshini n. sp. from Barbatula toni, Russia (A, C, D, F) and Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus, Russia (B, E, G, H). A, B — dorsoventral view, C — apical view, D — lateral view, E — subapical view, F — acicular filitriches and gladiate spinitriches on internal rim of suckers, G — acicular filitriches between suckers, H — gladiate spinitriches on neck region. Small letters in A and B indicate position of microtriches on F H)." httpUri="https://zenodo.org/record/886423/files/figure.png" pageId="7" pageNumber="878">Fig. 6</figureCitation>
G, and on scolex apex); neck covered with gladiate spinitriches (
<figureCitation box="[929,979,1467,1486]" captionStart="Fig. 6" captionStartId="9.[90,115,1331,1345]" captionTargetBox="[234,1330,144,1294]" captionTargetId="figure@9.[232,1332,142,1295]" captionTargetPageId="9" captionText="Fig. 6. Scanning electron micrograph of Proteocephalus demshini n. sp. from Barbatula toni, Russia (A, C, D, F) and Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus, Russia (B, E, G, H). A, B — dorsoventral view, C — apical view, D — lateral view, E — subapical view, F — acicular filitriches and gladiate spinitriches on internal rim of suckers, G — acicular filitriches between suckers, H — gladiate spinitriches on neck region. Small letters in A and B indicate position of microtriches on F H)." httpUri="https://zenodo.org/record/886423/files/figure.png" pageId="7" pageNumber="878">Fig. 6</figureCitation>
H). Inner longitudinal internal musculature well-developed, anastomosed. Ventral osmoregulatory canals thin-walled, 1012 wide, overlapping lateral bands of vitelline follicles, sometimes anastomosed, slightly sinuous (
<figureCitation box="[1108,1158,1550,1569]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
E). Dorsal osmoregulatory canals thick-walled, narrow, about 5 wide, difficult to observe in most mature or pregravid proglottids.
</paragraph>
<paragraph blockId="7.[805,1475,1243,1988]" pageId="7" pageNumber="878">
Testes
<quantity box="[904,991,1634,1653]" metricMagnitude="-1" metricUnit="m" metricValue="9.652" metricValueMax="11.176" metricValueMin="8.128" pageId="7" pageNumber="878" unit="in" value="38.0" valueMax="44.0" valueMin="32.0">3244 in</quantity>
number, spherical, small, in two irregular layers (
<figureCitation box="[814,869,1662,1681]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
E), forming one field separated aporally by well-developed sperm duct (vas deferens) (
<figureCitation box="[1072,1123,1690,1709]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
E). Testes present also in gravid proglottids.
<collectingRegion box="[890,924,1718,1737]" country="Hungary" name="Vas" pageId="7" pageNumber="878">Vas</collectingRegion>
deferens strongly coiled, with loops forming elongated field crossing median line of proglottids (
<figureCitation box="[1193,1242,1745,1765]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
D, E). Cirrus-sac ovoid to almost spherical, thick-walled; sperm duct (internal vas deferens) coiled. Cirrus muscular. Common genital atrium narrow, deep (
<figureCitation box="[1326,1374,1801,1820]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
D). Genital pores alternating irregularly, pre-equatorial to equatorial.
</paragraph>
<paragraph blockId="7.[805,1475,1243,1988]" pageId="7" pageNumber="878">
Ovary medullary, bilobed. Relative size of ovary (see [9]) about 15% of size of pregravid proglottids. Mehlis' gland
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diameter. Vaginal canal slightly sinuous in distal part; terminal part of vaginal canal (
<emphasis box="[876,1106,1939,1959]" italics="true" pageId="7" pageNumber="878">pars copulatrix vaginae</emphasis>
) surrounded by chromophilic cells, without sphincter (
<figureCitation box="[989,1039,1969,1988]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="7" pageNumber="878">Fig. 5</figureCitation>
D).
</paragraph>
<caption httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="8" pageNumber="879" targetBox="[238,1373,144,1679]" targetPageId="8">
<paragraph blockId="8.[113,1499,1715,1776]" pageId="8" pageNumber="879">
<emphasis bold="true" box="[113,159,1716,1730]" pageId="8" pageNumber="879">Fig. 5.</emphasis>
<taxonomicName authority="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach, 2017" authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[162,339,1715,1730]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="8" pageNumber="879" phylum="Platyhelminthes" rank="species" species="misgurni" status="n. sp.">
<emphasis box="[162,339,1715,1730]" italics="true" pageId="8" pageNumber="879">Proteocephalus misgurni</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[343,382,1717,1731]" pageId="8" pageNumber="879" rank="species">n. sp.</taxonomicNameLabel>
from
<taxonomicName box="[424,616,1715,1730]" class="Actinopterygii" family="Cobitidae" genus="Misgurnus" kingdom="Animalia" order="Cypriniformes" pageId="8" pageNumber="879" phylum="Chordata" rank="species" species="anguillicaudatus">
<emphasis box="[424,616,1715,1730]" italics="true" pageId="8" pageNumber="879">Misgurnus anguillicaudatus</emphasis>
</taxonomicName>
. A.Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E.Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated.
</paragraph>
</caption>
<paragraph blockId="8.[113,784,1857,1988]" lastBlockId="8.[828,1498,1857,1988]" pageId="8" pageNumber="879">
Vitelline follicles medullary, forming two long, narrow lateral bands slightly widened posteriorly at ovarian level (
<figureCitation box="[539,588,1885,1904]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="8" pageNumber="879">Fig. 5</figureCitation>
E). Follicles absent at level of cirrus-sac and vagina on ventral side (
<figureCitation box="[546,596,1913,1932]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="8" pageNumber="879">Fig. 5</figureCitation>
E). Posteriorly, follicles may overlap ovary ventrally (
<figureCitation box="[440,491,1941,1960]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="8" pageNumber="879">Fig. 5</figureCitation>
E). Uterus medullary, with development of
<typeStatus box="[253,296,1969,1988]" pageId="8" pageNumber="879">type</typeStatus>
2 according to [9]. Uterus occupies up to 38% of proglottid width in last immature proglottids, up to 87% in pregravid proglottids and up to 92% of gravid proglottids.
</paragraph>
<paragraph blockId="8.[828,1498,1857,1988]" pageId="8" pageNumber="879">
Eggs spherical, with hyaline outer envelope and bilayered embryophore; oncosphere containing six embryonic hooks 1517 long (
<figureCitation box="[884,934,1969,1988]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="8" pageNumber="879">Fig. 5</figureCitation>
B).
</paragraph>
<caption httpUri="https://zenodo.org/record/886423/files/figure.png" pageId="9" pageNumber="880" targetBox="[234,1330,144,1294]" targetPageId="9">
<paragraph blockId="9.[90,1475,1330,1391]" pageId="9" pageNumber="880">
<emphasis bold="true" box="[90,135,1331,1345]" pageId="9" pageNumber="880">Fig. 6.</emphasis>
Scanning electron micrograph of
<taxonomicName authority="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach, 2017" authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[384,562,1330,1345]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="demshini" status="n. sp.">
<emphasis box="[384,562,1330,1345]" italics="true" pageId="9" pageNumber="880">Proteocephalus demshini</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[565,605,1331,1345]" pageId="9" pageNumber="880" rank="species">n. sp.</taxonomicNameLabel>
from
<taxonomicName baseAuthorityName="Dybowski" baseAuthorityYear="1869" box="[647,749,1330,1345]" class="Actinopterygii" family="Nemacheilidae" genus="Barbatula" kingdom="Animalia" order="Cypriniformes" pageId="9" pageNumber="880" phylum="Chordata" rank="species" species="toni">
<emphasis box="[647,749,1330,1345]" italics="true" pageId="9" pageNumber="880">Barbatula toni</emphasis>
</taxonomicName>
, Russia (A, C,D,F) and
<taxonomicName authority="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach, 2017" authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[919,1096,1330,1345]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="misgurni" status="n. sp.">
<emphasis box="[919,1096,1330,1345]" italics="true" pageId="9" pageNumber="880">Proteocephalus misgurni</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[1099,1138,1331,1345]" pageId="9" pageNumber="880" rank="species">n. sp.</taxonomicNameLabel>
from
<taxonomicName box="[1180,1378,1330,1345]" class="Actinopterygii" family="Cobitidae" genus="Misgurnus" kingdom="Animalia" order="Cypriniformes" pageId="9" pageNumber="880" phylum="Chordata" rank="species" species="anguillicaudatus">
<emphasis box="[1180,1378,1330,1345]" italics="true" pageId="9" pageNumber="880">Misgurnus anguillicaudatus</emphasis>
</taxonomicName>
, Russia (B, E, G, H). A, B — dorsoventral view, C — apical view, D — lateral view, E — subapical view, F — acicular filitriches and gladiate spinitriches on internal rim of suckers, G — acicular filitriches between suckers, H — gladiate spinitriches on neck region. Small letters in A and B indicate position of microtriches on FH).
</paragraph>
</caption>
<subSection lastPageId="10" lastPageNumber="881" pageId="9" pageNumber="880" type="multiple">
<paragraph blockId="9.[90,760,1465,1988]" box="[90,280,1465,1485]" pageId="9" pageNumber="880">
<heading box="[90,280,1465,1485]" fontSize="8" level="2" pageId="9" pageNumber="880" reason="1">
<emphasis box="[90,280,1465,1485]" italics="true" pageId="9" pageNumber="880">Taxonomic summary</emphasis>
</heading>
</paragraph>
<subSubSection pageId="9" pageNumber="880" type="biology_ecology">
<paragraph blockId="9.[90,760,1465,1988]" pageId="9" pageNumber="880">
<emphasis box="[122,356,1493,1513]" italics="true" pageId="9" pageNumber="880">
<typeStatus box="[122,165,1493,1513]" pageId="9" pageNumber="880">Type</typeStatus>
and only known host
</emphasis>
:
<taxonomicName authority="(Cantor, 1842)" authorityName="Cantor" authorityYear="1842" baseAuthorityName="Cantor" baseAuthorityYear="1842" box="[368,760,1493,1514]" class="Actinopterygii" family="Cobitidae" genus="Misgurnus" kingdom="Animalia" order="Cypriniformes" pageId="9" pageNumber="880" phylum="Chordata" rank="species" species="anguillicaudatus">
<emphasis box="[368,616,1493,1513]" italics="true" pageId="9" pageNumber="880">
<taxonomicName box="[368,464,1493,1513]" class="Actinopterygii" family="Cobitidae" genus="Misgurnus" higherTaxonomySource="CoL" kingdom="Animalia" order="Cypriniformes" pageId="9" pageNumber="880" phylum="Chordata" rank="genus">Misgurnus</taxonomicName>
anguillicaudatus
</emphasis>
(Cantor,
<date box="[703,753,1495,1514]" pageId="9" pageNumber="880" value="1842">1842</date>
)
</taxonomicName>
(
<taxonomicName box="[96,238,1522,1541]" class="Actinopterygii" higherTaxonomySource="GBIF" kingdom="Animalia" order="Cypriniformes" pageId="9" pageNumber="880" phylum="Chordata" rank="order">Cypriniformes</taxonomicName>
:
<taxonomicName box="[246,340,1522,1541]" class="Actinopterygii" family="Cobitidae" kingdom="Animalia" order="Cypriniformes" pageId="9" pageNumber="880" phylum="Chordata" rank="family">Cobitidae</taxonomicName>
).
</paragraph>
<paragraph blockId="9.[90,760,1465,1988]" box="[122,362,1549,1570]" pageId="9" pageNumber="880">
<emphasis box="[122,261,1549,1569]" italics="true" pageId="9" pageNumber="880">Site of infection</emphasis>
: intestine.
</paragraph>
</subSubSection>
<subSubSection pageId="9" pageNumber="880" type="materials_examined">
<paragraph blockId="9.[90,760,1465,1988]" pageId="9" pageNumber="880">
<emphasis box="[122,235,1577,1597]" italics="true" pageId="9" pageNumber="880">
<typeStatus box="[122,165,1577,1597]" pageId="9" pageNumber="880">Type</typeStatus>
locality
</emphasis>
: River Ilistaya near Chernigovka,
<collectingRegion box="[542,639,1578,1597]" country="Russia" name="Primorskiy" pageId="9" pageNumber="880">Primorsky</collectingRegion>
Region,
<collectingCountry name="Russia" pageId="9" pageNumber="880">Russia</collectingCountry>
(44̊2647″N, 132̊2520″E).
</paragraph>
</subSubSection>
<subSubSection pageId="9" pageNumber="880" type="biology_ecology">
<paragraph blockId="9.[90,760,1465,1988]" pageId="9" pageNumber="880">
<emphasis box="[122,450,1633,1653]" italics="true" pageId="9" pageNumber="880">Prevalence and intensity of infection</emphasis>
(
<emphasis italics="true" pageId="9" pageNumber="880">
mean with range and number of
<emphasis bold="true" box="[90,102,1662,1681]" italics="true" pageId="9" pageNumber="880">fi</emphasis>
sh examined in parentheses
</emphasis>
): 29% (n = 73); 1.3 (13).
</paragraph>
</subSubSection>
<subSubSection pageId="9" pageNumber="880" type="materials_examined">
<paragraph blockId="9.[90,760,1465,1988]" pageId="9" pageNumber="880">
<emphasis box="[122,249,1688,1708]" italics="true" pageId="9" pageNumber="880">
<typeStatus box="[122,165,1688,1708]" pageId="9" pageNumber="880">Type</typeStatus>
material
</emphasis>
:
<typeStatus box="[262,350,1690,1709]" pageId="9" pageNumber="880">Holotype</typeStatus>
(one whole-mounted specimen from host field No. RUS 77 collected on
<date box="[394,532,1718,1737]" pageId="9" pageNumber="880" value="2011-06-21">21 June 2011</date>
; Coll. No. MHNG-PLAT 79136); two
<typeStatus box="[219,315,1746,1765]" pageId="9" pageNumber="880">paratypes</typeStatus>
(whole-mounted specimens from hosts field Nos. RUS 75 and RUS 100 collected on
<date box="[452,646,1773,1793]" pageId="9" pageNumber="880" value="2011-06-21" valueMax="2011-06-22" valueMin="2011-06-21">21 and 22 June 2011</date>
, respectively; MHNG-PLAT 79135 and 79142); two
<typeStatus box="[489,585,1802,1821]" pageId="9" pageNumber="880">paratypes</typeStatus>
(whole-mounted specimens from host field Nos. RUS 91 and 121 collected on
<date pageId="9" pageNumber="880" value="2011-06-22" valueMax="2011-06-23" valueMin="2011-06-22">22 and 23 June 2011</date>
, respectively;
<collectionCode box="[321,376,1857,1876]" pageId="9" pageNumber="880">IPCAS</collectionCode>
C-728).
</paragraph>
</subSubSection>
<subSubSection box="[122,760,1884,1904]" pageId="9" pageNumber="880" type="etymology">
<paragraph blockId="9.[90,760,1465,1988]" box="[122,760,1884,1904]" pageId="9" pageNumber="880">
<emphasis box="[122,217,1884,1904]" italics="true" pageId="9" pageNumber="880">Etymology</emphasis>
: the specific name is given according to the host's genus.
</paragraph>
</subSubSection>
<subSubSection lastPageId="10" lastPageNumber="881" pageId="9" pageNumber="880" type="diagnosis">
<paragraph blockId="9.[90,760,1465,1988]" lastBlockId="9.[805,1475,1466,1988]" pageId="9" pageNumber="880">
<emphasis box="[122,572,1912,1932]" italics="true" pageId="9" pageNumber="880">
Differential diagnosis.
<taxonomicName authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[338,572,1912,1932]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="misgurni">
<taxonomicName box="[338,481,1912,1932]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" higherTaxonomySource="CoL" kingdom="Animalia" order="Onchoproteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="genus">Proteocephalus</taxonomicName>
misgurni
</taxonomicName>
</emphasis>
is most similar to
<taxonomicName authorityName="Shimazu" authorityYear="1990" box="[90,218,1939,1959]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="midoriensis">
<emphasis box="[90,218,1939,1959]" italics="true" pageId="9" pageNumber="880">P. midoriensis</emphasis>
</taxonomicName>
(see below), and differs from
<taxonomicName authorityName="La Rue" authorityYear="1911" baseAuthorityName="Grimm" baseAuthorityYear="1872" box="[509,601,1939,1959]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="sagittus">
<emphasis box="[509,601,1939,1959]" italics="true" pageId="9" pageNumber="880">P. sagittus</emphasis>
</taxonomicName>
and
<taxonomicName authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[649,755,1939,1959]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="demshini">
<emphasis box="[649,755,1939,1959]" italics="true" pageId="9" pageNumber="880">P. demshini</emphasis>
</taxonomicName>
, in a similar shape of the body, with the club-shaped scolex conspicuously wider than immature and mature proglottids, the strobila composed of well-separated proglottids that are rectangular (most immature ones) or longer than wide (all remaining proglottids
<figureCitation box="[825,878,1550,1569]" captionStart="Fig. 5" captionStartId="8.[113,138,1716,1730]" captionTargetBox="[238,1373,144,1679]" captionTargetId="figure@8.[236,1375,142,1680]" captionTargetPageId="8" captionText="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri="https://zenodo.org/record/886421/files/figure.png" pageId="9" pageNumber="880">Fig. 5</figureCitation>
<collectionCodeEnum box="[878,896,1550,1569]" pageId="9" pageNumber="880">A</collectionCodeEnum>
), relatively few vitelline follicles that form narrow lateral bands, with posterior follicles overlapping the ovarian wings on the ventral side, a subspherical to almost spherical cirrus-sac, and low number of testes (
<emphasis box="[935,947,1634,1653]" italics="true" pageId="9" pageNumber="880">&lt;</emphasis>
50) (
<figureCitation box="[997,1079,1634,1653]" captionStart-0="Fig. 2" captionStart-1="Fig. 3" captionStart-2="Fig. 4" captionStart-3="Fig. 5" captionStartId-0="4.[113,138,775,789]" captionStartId-1="6.[113,139,1572,1586]" captionStartId-2="7.[90,115,1130,1144]" captionStartId-3="8.[113,138,1716,1730]" captionTargetBox-0="[333,1278,144,738]" captionTargetBox-1="[257,1353,144,1536]" captionTargetBox-2="[234,1330,144,1093]" captionTargetBox-3="[238,1373,144,1679]" captionTargetId-0="figure@4.[331,1280,142,739]" captionTargetId-1="figure@6.[255,1355,142,1537]" captionTargetId-2="figure@7.[232,1332,142,1094]" captionTargetId-3="figure@8.[236,1375,142,1680]" captionTargetPageId-0="4" captionTargetPageId-1="6" captionTargetPageId-2="7" captionTargetPageId-3="8" captionText-0="Fig. 2. Proteocephalus sagittus (Grimm, 1872) from Barbatula barbatula, Czech Republic (IPCAS C- 33 / 1). A. Scolex. B. Mature proglottid. Abbreviations (for all figures): cc = chromophilic cells; ci = cirrus; cp = pars copulatrix vaginae; cs = cirrus-sac; doc = dorsal osmoregulatory canal; du = uterine diverticles; eh = embryonic hooks; em = bilayered embryophore; ga = genital atrium; gc = glandular cells, probably of exocrine type; lm = internal longitudinal musculature; ln = longitudinal lateral nerves; Mg = Mehlis' gland; oe = outer envelope; on = oncosphere; oo = oocapt; ov = ovary; sc = subtegumental cells; sd = sperm duct (vas deferens); st = subtegumental muscle fibres; te = testes; tg = tegument; ud = uterine ducts; us = uterine stem; ut = uterus; vc = vaginal canal; vd = vitelloduct; vf = vitelline follicles; voc = ventral osmoregulatory canal; vs = vaginal sphincter." captionText-1="Fig. 3. Proteocephalus midoriensis Shimazu, 1990 from Lefua echigonia, Japan (IPCAS C- 362 / 1) (A, C E) and from Lefua costata, Russia (“ Proteocephalus sp. n. ” of N. I. Demshin; not accessioned) (B). A, B. Scoleces. C. Mature proglottid, dorsal view, note vitelline follicles overpassing the ovary ventrally; uterus not illustrated. D. Detail of the posterior part of the proglottid, ventral view. E. Detail of terminal genitalia, ventral view." captionText-2="Fig. 4. Proteocephalus demshini n. sp. from Barbatula toni, Russia. A. Scolex (host field No. RUS 195; IPCAS C- 727). B. Mature proglottid, ventral view (host field No. RUS 191; IPCAS C- 727); uterus not illustrated. C. Detail of terminal genitalia, ventral view (host field No. RUS 192; MHNG-PLAT 79149). D. Eggs drawn in distilled water (host field No. RUS 190; IPCAS C- 727)." captionText-3="Fig. 5. Proteocephalus misgurni n. sp. from Misgurnus anguillicaudatus. A. Holotype, total view, ventrally (host field No. RUS 77; MHNG-PLAT 79136). B. Eggs drawn in distilled water (host field No. RUS 93; MHNG-PLAT 79140). C. Paratype 1, scolex (host field No. RUS 100; MHNG-PLAT 79142); note the proliferation zone widened anteriorly. D. Paratype 2, detail of terminal genitalia, dorsal view (host field No. RUS 75; MHNG-PLAT 79135); uterus not illustrated. E. Holotype, mature proglottid, ventral view (host field No. RUS 77; MHNG-PLAT 79136); uterus not illustrated." httpUri-0="https://zenodo.org/record/886413/files/figure.png" httpUri-1="https://zenodo.org/record/886415/files/figure.png" httpUri-2="https://zenodo.org/record/886419/files/figure.png" httpUri-3="https://zenodo.org/record/886421/files/figure.png" pageId="9" pageNumber="880">Figs. 25</figureCitation>
;
<tableCitation box="[1092,1160,1634,1653]" captionStart="Table 2" captionStartId="5.[90,133,157,171]" captionText="Table 2 Comparative measurements of species of Proteocephalus (Cestoda: Onchoproteocephalidea) from loaches (Cobitoidea) in the Palaearctic region. Measurements in micrometres unless oth- erwise stated." pageId="9" pageNumber="880">Table 2</tableCitation>
).
</paragraph>
<paragraph blockId="9.[805,1475,1466,1988]" lastBlockId="10.[113,784,155,733]" lastPageId="10" lastPageNumber="881" pageId="9" pageNumber="880">
This new species can be easily distinguished from
<taxonomicName box="[1346,1474,1660,1680]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="midorensis">
<emphasis box="[1346,1474,1660,1680]" italics="true" pageId="9" pageNumber="880">
<collectionCodeEnum box="[1346,1360,1660,1680]" pageId="9" pageNumber="880">P</collectionCodeEnum>
. midorensis
</emphasis>
</taxonomicName>
by (i) its size (it is about twice longer and wider see
<tableCitation box="[1389,1465,1690,1709]" captionStart="Table 2" captionStartId="5.[90,133,157,171]" captionText="Table 2 Comparative measurements of species of Proteocephalus (Cestoda: Onchoproteocephalidea) from loaches (Cobitoidea) in the Palaearctic region. Measurements in micrometres unless oth- erwise stated." pageId="9" pageNumber="880">Table 2</tableCitation>
) including a much wider scolex (width 425535 μm versus 285 310 μm); (ii) less elongate proglottids (see
<figureCitation box="[1255,1320,1745,1765]" captionStart="Fig. 3" captionStartId="6.[113,139,1572,1586]" captionTargetBox="[257,1353,144,1536]" captionTargetId="figure@6.[255,1355,142,1537]" captionTargetPageId="6" captionText="Fig. 3. Proteocephalus midoriensis Shimazu, 1990 from Lefua echigonia, Japan (IPCAS C- 362 / 1) (A, C E) and from Lefua costata, Russia (“ Proteocephalus sp. n. ” of N. I. Demshin; not accessioned) (B). A, B. Scoleces. C. Mature proglottid, dorsal view, note vitelline follicles overpassing the ovary ventrally; uterus not illustrated. D. Detail of the posterior part of the proglottid, ventral view. E. Detail of terminal genitalia, ventral view." httpUri="https://zenodo.org/record/886415/files/figure.png" pageId="9" pageNumber="880">Figs. 3</figureCitation>
<collectionCodeEnum box="[1320,1333,1746,1765]" pageId="9" pageNumber="880">C</collectionCodeEnum>
and 5
<collectionCodeEnum box="[1397,1415,1746,1765]" pageId="9" pageNumber="880">E</collectionCodeEnum>
); (iii) longer (horizontally) and narrower (in vertical direction, i.e. height) lateral wings of the ovary versus wings longer than wide in
<taxonomicName authorityName="Shimazu" authorityYear="1990" box="[830,965,1828,1848]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="midoriensis">
<emphasis box="[830,965,1828,1848]" italics="true" pageId="9" pageNumber="880">
<collectionCodeEnum box="[830,844,1828,1848]" pageId="9" pageNumber="880">P</collectionCodeEnum>
. midoriensis
</emphasis>
</taxonomicName>
(
<figureCitation box="[980,1044,1829,1848]" captionStart="Fig. 3" captionStartId="6.[113,139,1572,1586]" captionTargetBox="[257,1353,144,1536]" captionTargetId="figure@6.[255,1355,142,1537]" captionTargetPageId="6" captionText="Fig. 3. Proteocephalus midoriensis Shimazu, 1990 from Lefua echigonia, Japan (IPCAS C- 362 / 1) (A, C E) and from Lefua costata, Russia (“ Proteocephalus sp. n. ” of N. I. Demshin; not accessioned) (B). A, B. Scoleces. C. Mature proglottid, dorsal view, note vitelline follicles overpassing the ovary ventrally; uterus not illustrated. D. Detail of the posterior part of the proglottid, ventral view. E. Detail of terminal genitalia, ventral view." httpUri="https://zenodo.org/record/886415/files/figure.png" pageId="9" pageNumber="880">Figs. 3</figureCitation>
<collectionCodeEnum box="[1043,1056,1829,1848]" pageId="9" pageNumber="880">C</collectionCodeEnum>
and 5
<collectionCodeEnum box="[1118,1136,1829,1848]" pageId="9" pageNumber="880">E</collectionCodeEnum>
); (iv) posterior extent of vitelline follicles, which overlap the ovary ventrally only up to its fifth in this new species (versus up to its half in
<taxonomicName authorityName="Shimazu" authorityYear="1990" box="[1216,1350,1884,1904]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="midoriensis">
<emphasis box="[1216,1350,1884,1904]" italics="true" pageId="9" pageNumber="880">
<collectionCodeEnum box="[1216,1230,1884,1904]" pageId="9" pageNumber="880">P</collectionCodeEnum>
. midoriensis
</emphasis>
</taxonomicName>
), and width of their lateral bands (bands represent only 1012% of the width of proglottids in
<taxonomicName authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[976,1087,1939,1959]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="misgurni">
<emphasis box="[976,1087,1939,1959]" italics="true" pageId="9" pageNumber="880">
<collectionCodeEnum box="[976,990,1939,1959]" pageId="9" pageNumber="880">P</collectionCodeEnum>
. misgurni
</emphasis>
</taxonomicName>
versus 1922% in
<taxonomicName authorityName="Shimazu" authorityYear="1990" box="[1275,1413,1939,1959]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="9" pageNumber="880" phylum="Platyhelminthes" rank="species" species="midoriensis">
<emphasis box="[1275,1413,1939,1959]" italics="true" pageId="9" pageNumber="880">
<collectionCodeEnum box="[1275,1289,1939,1959]" pageId="9" pageNumber="880">P</collectionCodeEnum>
. midoriensis
</emphasis>
</taxonomicName>
); and (v) other metrical data such as the diameter of oncospheres, relative size of the suckers and relative position of the genital pore (see
<tableCitation box="[162,234,183,202]" captionStart="Table 2" captionStartId="5.[90,133,157,171]" captionText="Table 2 Comparative measurements of species of Proteocephalus (Cestoda: Onchoproteocephalidea) from loaches (Cobitoidea) in the Palaearctic region. Measurements in micrometres unless oth- erwise stated." pageId="10" pageNumber="881">Table 2</tableCitation>
).
</paragraph>
</subSubSection>
<subSubSection pageId="10" pageNumber="881" type="discussion">
<paragraph blockId="10.[113,784,155,733]" pageId="10" pageNumber="881">
Proposal of
<taxonomicName authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[250,347,210,230]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="10" pageNumber="881" phylum="Platyhelminthes" rank="species" species="misgurni">
<emphasis box="[250,347,210,230]" italics="true" pageId="10" pageNumber="881">
<collectionCodeEnum box="[250,263,210,230]" pageId="10" pageNumber="881">P</collectionCodeEnum>
.misgurni
</emphasis>
</taxonomicName>
as a new species is supported by molecular data (see
<figureCitation box="[157,204,239,258]" captionStart="Fig. 1" captionStartId="3.[90,115,1452,1466]" captionTargetBox="[96,1466,147,1414]" captionTargetId="figure@3.[638,1298,175,1414]" captionTargetPageId="3" captionText="Fig. 1. Bayesian analysis of the concatenated 4 - gene alignment (ssrDNA, lsrDNA, rrnL, cox 1) constructed using MrBayes 3.2. 1 using the GTR + I + G (ssrDNA, rrnL, cox 1) and GTR + I (lsrDNA) models of nucleotide evolution. The analysis was run for 10 million generations; 7 million generations were discarded as burn-in. Posterior probabilities and maximum likelihood bootstrap values (obtained using GARLI) are shown above and below branches, respectively. All nodes with &lt;0.95 posterior probabilities have been collapsed. The branchlength scale indicates number of substitutions per site." httpUri="https://zenodo.org/record/886411/files/figure.png" pageId="10" pageNumber="881">Fig.1</figureCitation>
, Suppl.
<figureCitation box="[274,330,239,258]" captionStart="Fig. 1" captionStartId="3.[90,115,1452,1466]" captionTargetBox="[96,1466,147,1414]" captionTargetId="figure@3.[638,1298,175,1414]" captionTargetPageId="3" captionText="Fig. 1. Bayesian analysis of the concatenated 4 - gene alignment (ssrDNA, lsrDNA, rrnL, cox 1) constructed using MrBayes 3.2. 1 using the GTR + I + G (ssrDNA, rrnL, cox 1) and GTR + I (lsrDNA) models of nucleotide evolution. The analysis was run for 10 million generations; 7 million generations were discarded as burn-in. Posterior probabilities and maximum likelihood bootstrap values (obtained using GARLI) are shown above and below branches, respectively. All nodes with &lt;0.95 posterior probabilities have been collapsed. The branchlength scale indicates number of substitutions per site." httpUri="https://zenodo.org/record/886411/files/figure.png" pageId="10" pageNumber="881">Figs. 1</figureCitation>
,
<figureCitation box="[339,351,239,258]" captionStart="Fig. 2" captionStartId="4.[113,138,775,789]" captionTargetBox="[333,1278,144,738]" captionTargetId="figure@4.[331,1280,142,739]" captionTargetPageId="4" captionText="Fig. 2. Proteocephalus sagittus (Grimm, 1872) from Barbatula barbatula, Czech Republic (IPCAS C- 33 / 1). A. Scolex. B. Mature proglottid. Abbreviations (for all figures): cc = chromophilic cells; ci = cirrus; cp = pars copulatrix vaginae; cs = cirrus-sac; doc = dorsal osmoregulatory canal; du = uterine diverticles; eh = embryonic hooks; em = bilayered embryophore; ga = genital atrium; gc = glandular cells, probably of exocrine type; lm = internal longitudinal musculature; ln = longitudinal lateral nerves; Mg = Mehlis' gland; oe = outer envelope; on = oncosphere; oo = oocapt; ov = ovary; sc = subtegumental cells; sd = sperm duct (vas deferens); st = subtegumental muscle fibres; te = testes; tg = tegument; ud = uterine ducts; us = uterine stem; ut = uterus; vc = vaginal canal; vd = vitelloduct; vf = vitelline follicles; voc = ventral osmoregulatory canal; vs = vaginal sphincter." httpUri="https://zenodo.org/record/886413/files/figure.png" pageId="10" pageNumber="881">2</figureCitation>
,
<figureCitation box="[360,373,239,258]" captionStart="Fig. 4" captionStartId="7.[90,115,1130,1144]" captionTargetBox="[234,1330,144,1093]" captionTargetId="figure@7.[232,1332,142,1094]" captionTargetPageId="7" captionText="Fig. 4. Proteocephalus demshini n. sp. from Barbatula toni, Russia. A. Scolex (host field No. RUS 195; IPCAS C- 727). B. Mature proglottid, ventral view (host field No. RUS 191; IPCAS C- 727); uterus not illustrated. C. Detail of terminal genitalia, ventral view (host field No. RUS 192; MHNG-PLAT 79149). D. Eggs drawn in distilled water (host field No. RUS 190; IPCAS C- 727)." httpUri="https://zenodo.org/record/886419/files/figure.png" pageId="10" pageNumber="881">4</figureCitation>
, Suppl.
<tableCitation box="[442,507,239,258]" captionStart="Table 1" captionStartId="2.[113,156,157,171]" captionText="Table 1" pageId="10" pageNumber="881">Table 1</tableCitation>
); uncorrected p-distances between this species and its sister species,
<taxonomicName authorityName="Shimazu" authorityYear="1990" box="[477,597,266,286]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="10" pageNumber="881" phylum="Platyhelminthes" rank="species" species="midoriensis">
<emphasis box="[477,597,266,286]" italics="true" pageId="10" pageNumber="881">
<collectionCodeEnum box="[477,490,266,286]" pageId="10" pageNumber="881">P</collectionCodeEnum>
.midoriensis
</emphasis>
</taxonomicName>
, are 1.5% for
<emphasis box="[713,778,266,286]" italics="true" pageId="10" pageNumber="881">ssrDNA</emphasis>
, 0.6% for
<emphasis box="[191,253,294,314]" italics="true" pageId="10" pageNumber="881">lsrDNA</emphasis>
and 2.5% for
<emphasis box="[376,413,294,314]" italics="true" pageId="10" pageNumber="881">rrnL</emphasis>
, respectively (Suppl.
<tableCitation box="[610,676,295,314]" captionStart="Table 1" captionStartId="2.[113,156,157,171]" captionText="Table 1" pageId="10" pageNumber="881">Table 1</tableCitation>
).
</paragraph>
<paragraph blockId="10.[113,784,155,733]" pageId="10" pageNumber="881">
This new species seems to be a specific parasite of
<taxonomicName baseAuthorityName="Cantor" baseAuthorityYear="1842" class="Actinopterygii" family="Cobitidae" genus="Misgurnus" kingdom="Animalia" order="Cypriniformes" pageId="10" pageNumber="881" phylum="Chordata" rank="species" species="anguillicaudatus">
<emphasis italics="true" pageId="10" pageNumber="881">
<collectionCodeEnum box="[759,779,322,342]" pageId="10" pageNumber="881">M</collectionCodeEnum>
. anguillicaudatus
</emphasis>
</taxonomicName>
, which is native to Siberia (Tugur and
<collectingRegion box="[620,674,351,370]" country="Russia" name="Amur" pageId="10" pageNumber="881">Amur</collectingRegion>
drainages),
<collectingRegion box="[113,196,379,398]" country="Russia" name="Sakhalin" pageId="10" pageNumber="881">Sakhalin</collectingRegion>
,
<collectingCountry box="[205,262,379,398]" name="South Korea" pageId="10" pageNumber="881">Korea</collectingCountry>
,
<collectingCountry box="[271,325,379,398]" name="Japan" pageId="10" pageNumber="881">Japan</collectingCountry>
,
<collectingCountry box="[334,389,379,398]" name="China" pageId="10" pageNumber="881">China</collectingCountry>
south to northern
<collectingCountry box="[569,651,379,398]" name="Vietnam" pageId="10" pageNumber="881">Vietnam</collectingCountry>
, but has been introduced to several localities in the River Rhine (
<collectingCountry box="[582,675,407,426]" name="Germany" pageId="10" pageNumber="881">Germany</collectingCountry>
) and
<collectingRegion box="[726,784,407,426]" country="Switzerland" name="Ticino" pageId="10" pageNumber="881">Ticino</collectingRegion>
(
<collectingCountry box="[120,167,434,453]" name="Italy" pageId="10" pageNumber="881">Italy</collectingCountry>
, north of Milano) drainages, the Aral Sea basin, North America,
<collectingCountry box="[113,199,462,481]" name="Australia" pageId="10" pageNumber="881">Australia</collectingCountry>
and
<collectingRegion box="[248,318,462,481]" country="United States of America" name="Hawaii" pageId="10" pageNumber="881">Hawaii</collectingRegion>
. This species proved successful in the aquarium fish trade and at least one country reports adverse ecological impact after introduction [1,2]. The parasite fauna of weather loaches is rather depauperate and cestodes were represented only by species of the caryophyllidean genus
<taxonomicName authorityName="Kulakovskaya" authorityYear="1961" box="[341,526,573,593]" class="Cestoda" family="Caryophyllaeidae" genus="Paracaryophyllaeus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Caryophyllidea" pageId="10" pageNumber="881" phylum="Platyhelminthes" rank="genus">
<emphasis box="[341,526,573,593]" italics="true" pageId="10" pageNumber="881">Paracaryophyllaeus</emphasis>
</taxonomicName>
(see [4]). The present authors and their co-workers (
<collectionCodeEnum box="[381,397,602,621]" pageId="10" pageNumber="881">T</collectionCodeEnum>
. Shimazu in
<collectingCountry box="[516,569,602,621]" name="Japan" pageId="10" pageNumber="881">Japan</collectingCountry>
and
<collectionCodeEnum box="[616,630,602,621]" pageId="10" pageNumber="881">T</collectionCodeEnum>
. Scholz,
<collectionCodeEnum box="[709,730,602,621]" pageId="10" pageNumber="881">M</collectionCodeEnum>
. Oros and his Slovak co-workers in
<collectingCountry box="[400,458,630,649]" name="China" pageId="10" pageNumber="881">China</collectingCountry>
) examined as many as about 500 and 700 weather loaches in
<collectingCountry box="[405,459,658,677]" name="Japan" pageId="10" pageNumber="881">Japan</collectingCountry>
and
<collectingCountry box="[514,571,658,677]" name="China" pageId="10" pageNumber="881">China</collectingCountry>
, respectively, but no proteocephalidean cestodes were found. This indicates that
<taxonomicName authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[681,783,684,704]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="10" pageNumber="881" phylum="Platyhelminthes" rank="species" species="misgurni">
<emphasis box="[681,783,684,704]" italics="true" pageId="10" pageNumber="881">
<collectionCodeEnum box="[681,694,684,704]" pageId="10" pageNumber="881">P</collectionCodeEnum>
. misgurni
</emphasis>
</taxonomicName>
is also endemic to the
<collectingRegion box="[325,422,713,732]" country="Russia" name="Primorskiy" pageId="10" pageNumber="881">Primorsky</collectingRegion>
Region of
<collectingCountry box="[522,582,713,732]" name="Russia" pageId="10" pageNumber="881">Russia</collectingCountry>
as
<taxonomicName authorityName="Tomáš Scholz &amp; Alain de Chambrier &amp; Takeshi Shimazu &amp; Alexey Ermolenko &amp; Andrea Waeschenbach" authorityYear="2017" box="[613,716,712,732]" class="Cestoda" family="Proteocephalidae" genus="Proteocephalus" kingdom="Animalia" order="Proteocephalidea" pageId="10" pageNumber="881" phylum="Platyhelminthes" rank="species" species="demshini">
<emphasis box="[613,716,712,732]" italics="true" pageId="10" pageNumber="881">
<collectionCodeEnum box="[613,626,712,732]" pageId="10" pageNumber="881">P</collectionCodeEnum>
. demshini
</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</subSection>
</subSubSection>
</heading>
</treatment>
</subSection>
</document>