287 lines
19 KiB
XML
287 lines
19 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.95.87121" ID-Pensoft-Pub="1314-4049-95-163" ID-Pensoft-UUID="9D55E0913AFE50E38B5E9A119F266467" ModsDocID="1314-4049-95-163" checkinTime="1677176310751" checkinUser="pensoft" docAuthor="Liu, Jia-Wei, Manawasinghe, Ishara S., Liao, Xuan-Ni, Mao, Jin, Dong, Zhang-Yong, Jayawardena, Ruvishika S., Wanasinghe, Dhanushka N., Shu, Yong-Xin & Luo, Mei" docDate="2023" docId="57D630D6F8DD54708F0F08F809AB3BCE" docLanguage="en" docName="MycoKeys 95: 163-188" docOrigin="MycoKeys 95" docPubDate="2023-02-23" docSource="http://dx.doi.org/10.3897/mycokeys.95.87121" docTitle="Colletotrichum tomentosae J. W. Liu, Manawas. & M. Luo 2023, sp. nov." docType="treatment" docVersion="1" id="9D55E0913AFE50E38B5E9A119F266467" lastPageNumber="163" masterDocId="9D55E0913AFE50E38B5E9A119F266467" masterDocTitle="Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrus grandis cv. " Tomentosa " in China" masterLastPageNumber="188" masterPageNumber="163" pageNumber="163" updateTime="1677176310751" updateUser="pensoft">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrus grandis cv. " Tomentosa " in China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Liu, Jia-Wei</mods:namePart>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Manawasinghe, Ishara S.</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-5730-3596</mods:nameIdentifier>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China</mods:affiliation>
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<mods:nameIdentifier type="email">ishara9017@gmail.com</mods:nameIdentifier>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Liao, Xuan-Ni</mods:namePart>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Mao, Jin</mods:namePart>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Dong, Zhang-Yong</mods:namePart>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-7702-4885</mods:nameIdentifier>
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<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wanasinghe, Dhanushka N.</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0003-1759-3933</mods:nameIdentifier>
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<mods:affiliation>School of Science, Mae Fah Luang University, Chiang Rai, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Shu, Yong-Xin</mods:namePart>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Luo, Mei</mods:namePart>
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<mods:affiliation>Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China</mods:affiliation>
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<mods:nameIdentifier type="email">08luomei@163.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2023</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2023-02-23</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>95</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>163</mods:start>
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<mods:end>188</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.95.87121</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.95.87121</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-95-163</mods:identifier>
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<mods:identifier type="Pensoft-UUID">9D55E0913AFE50E38B5E9A119F266467</mods:identifier>
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</mods:mods>
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<treatment LSID="urn:lsid:plazi:treatment:57D630D6F8DD54708F0F08F809AB3BCE" httpUri="http://treatment.plazi.org/id/57D630D6F8DD54708F0F08F809AB3BCE" lastPageNumber="163" pageId="0" pageNumber="163">
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||
<subSubSection pageId="0" pageNumber="163" type="nomenclature">
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||
<paragraph pageId="0" pageNumber="163">
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<taxonomicName LSID="57D630D6-F8DD-5470-8F0F-08F809AB3BCE" authority="J. W. Liu, Manawas. & M. Luo" authorityName="J. W. Liu, Manawas. & M. Luo" authorityYear="2023" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum tomentosae" order="Glomerellales" pageId="0" pageNumber="163" phylum="Ascomycota" rank="species" species="tomentosae" status="sp. nov.">Colletotrichum tomentosae J.W. Liu, Manawas. & M. Luo</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="163">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="description">
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<paragraph pageId="0" pageNumber="163">
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Colletotrichum tomentosae (ZHKUCC 21 - 0103, holotype) A, B upper and reverse side of cultures on PDA seven days after inoculation C, D conidiophores with developing conidia E, F conidia G, H appressoria. Scale bars: 10 μm (C-H)." figureDoi="10.3897/mycokeys.95.87121.figure2" httpUri="https://binary.pensoft.net/fig/814953" pageId="0" pageNumber="163">Fig. 2</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="etymology">
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<paragraph pageId="0" pageNumber="163">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="163">
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The epithet refers to the cultivar of the host plant -
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<taxonomicName class="Magnoliopsida" family="Rutaceae" genus="Citrus" kingdom="Plantae" lsidName="Citrus grandis" order="Sapindales" pageId="0" pageNumber="163" phylum="Tracheophyta" rank="species" species="grandis">
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<emphasis italics="true" pageId="0" pageNumber="163">Citrus grandis</emphasis>
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</taxonomicName>
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cv.
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<normalizedToken originalValue="“Tomentosa”">"Tomentosa"</normalizedToken>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="holotype">
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<paragraph pageId="0" pageNumber="163">Holotype.</paragraph>
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<paragraph pageId="0" pageNumber="163">ZHKUCC 21-0103.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="description">
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<paragraph pageId="0" pageNumber="163">Description.</paragraph>
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<paragraph pageId="0" pageNumber="163">
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Endophytic in
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<taxonomicName lsidName="C. grandis" pageId="0" pageNumber="163" rank="species" species="grandis">
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<emphasis italics="true" pageId="0" pageNumber="163">C. grandis</emphasis>
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</taxonomicName>
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cv.
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<normalizedToken originalValue="“Tomentosa”">"Tomentosa"</normalizedToken>
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leaf.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="163">Sexual morph</emphasis>
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: not observed.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="163">Asexual morph</emphasis>
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: Conidiophores 20-40
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<normalizedToken originalValue="×">x</normalizedToken>
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3-5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(x- = 29.8
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<normalizedToken originalValue="±">+/-</normalizedToken>
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5.5
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<normalizedToken originalValue="×">x</normalizedToken>
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3.7
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<normalizedToken originalValue="±">+/-</normalizedToken>
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0.6
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 30), hyaline, cylindrical, 1-3-celled, unbranched or branched at the base. Conidia 10-20
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<normalizedToken originalValue="×">x</normalizedToken>
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3-6
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(x- = 12.5
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<normalizedToken originalValue="±">+/-</normalizedToken>
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1.6
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<normalizedToken originalValue="×">x</normalizedToken>
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4.4
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<normalizedToken originalValue="±">+/-</normalizedToken>
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0.6
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 50), 1-2-guttulate, aseptate, straight, hyaline, smooth-walled, middle part cylindrical both ends obtuse, middle part occasionally shrinkage or bulging. Appressoria 5-15
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<normalizedToken originalValue="×">x</normalizedToken>
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5-10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(x- = 10
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<normalizedToken originalValue="±">+/-</normalizedToken>
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1.8
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<normalizedToken originalValue="×">x</normalizedToken>
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7
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<normalizedToken originalValue="±">+/-</normalizedToken>
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1.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 50) solitary or in loose groups, light brown to medium brown, Ellipsoidal to subcircular or irregular-shaped.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="description">
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<paragraph pageId="0" pageNumber="163">Cultural characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="163">Colonies on PDA reach 70 mm diam. in seven days, with 10-11 mm/day (x- = 10 mm, n = 6) growth rate. Colonies flat with entire margin, floccose cottony, surface grey in the centre with glaucous margin. Reverse buff in the centre with off-white margin.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="materials_examined">
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<paragraph pageId="0" pageNumber="163">Material examined.</paragraph>
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<paragraph pageId="0" pageNumber="163">
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<materialsCitation collectingDate="2019-05" collectorName="Y. X. Shu" country="China" location="Huazhou" specimenCount="1" stateProvince="Guangdong Province" typeStatus="holotype">
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<collectingCountry name="China">China</collectingCountry>
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,
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<collectingRegion country="China" name="Guangdong">Guangdong Province</collectingRegion>
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,
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<location LSID="urn:lsid:plazi:treatment:57D630D6F8DD54708F0F08F809AB3BCE:D944F10D3C08D02500E3E4800C7F4A9C" country="China" name="Huazhou" stateProvince="Guangdong Province">Huazhou</location>
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, isolated from a healthy leaf of
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<taxonomicName class="Magnoliopsida" family="Rutaceae" genus="Citrus" kingdom="Plantae" lsidName="Citrus grandis" order="Sapindales" pageId="0" pageNumber="163" phylum="Tracheophyta" rank="species" species="grandis">
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<emphasis italics="true" pageId="0" pageNumber="163">Citrus grandis</emphasis>
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</taxonomicName>
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cv.
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<normalizedToken originalValue="“Tomentosa”">"Tomentosa"</normalizedToken>
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,
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<collectingDate value="2019-05">May 2019</collectingDate>
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,
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<collectorName>Y.X. Shu</collectorName>
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, (dried cultures ZHKU 21-0088
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<emphasis bold="true" italics="true" pageId="0" pageNumber="163">
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<typeStatus>holotype</typeStatus>
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</emphasis>
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); ex-type culture ZHKUCC 21-0103 (= CGMCC 3.24128), ex-isotype ZHKUCC 21-0104, ZHKUCC 22-0041
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</materialsCitation>
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).
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="163" type="notes">
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<paragraph pageId="0" pageNumber="163">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="163">
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In the phylogenetic analysis of combined six genes,
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<taxonomicName authorityName="J. W. Liu, Manawas. & M. Luo" authorityYear="2023" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum tomentosae" order="Glomerellales" pageId="0" pageNumber="163" phylum="Ascomycota" rank="species" species="tomentosae">
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<emphasis italics="true" pageId="0" pageNumber="163">Colletotrichum tomentosae</emphasis>
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</taxonomicName>
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formed an independent clade (Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. The most parsimonious tree of the Colletotrichum gloeosporioides complex developed using combined act, chs- 1, gapdh, ITS, β- tubulin and gs sequences. Colletotrichum boninense and C. hippeastri were used as outgroup taxa. Bootstrap values equal to or greater than 60 % in MP and ML and BP equal to or greater than 0.95 are shown as MP / ML / BP above the respective node. The isolates belonging to the current study are given in blue for known species and new species are shown in red. Ex-type strains are noted with T." figureDoi="10.3897/mycokeys.95.87121.figure1" httpUri="https://binary.pensoft.net/fig/814952" pageId="0" pageNumber="163">1</figureCitation>
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). This species is phylogenetically distinct from
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<taxonomicName lsidName="C. syzygicola" pageId="0" pageNumber="163" rank="species" species="syzygicola">
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<emphasis italics="true" pageId="0" pageNumber="163">C. syzygicola</emphasis>
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</taxonomicName>
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. Morphologically, appressoria developed by
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<taxonomicName lsidName="C. syzygicola" pageId="0" pageNumber="163" rank="species" species="syzygicola">
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<emphasis italics="true" pageId="0" pageNumber="163">C. syzygicola</emphasis>
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</taxonomicName>
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(DNCL021;
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<bibRefCitation DOI="https://doi.org/10.1007/s13225-013-0257-2" author="Udayanga, D" journalOrPublisher="The New Phytologist" pageId="0" pageNumber="163" refId="B46" refString="Udayanga, D, Manamgoda, DS, Liu, X, Chukeatirote, E, Hyde, KD, 2013. What are the common anthracnose pathogens of tropical fruits? Fungal Diversity 61(1): 165-179. https://doi.org/10.1007/s13225-013-0257-2" title="What are the common anthracnose pathogens of tropical fruits? Fungal Diversity 61 (1): 165 - 179." url="https://doi.org/10.1007/s13225-013-0257-2" year="2013">Udayanga et al. (2013)</bibRefCitation>
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) are longer than
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<taxonomicName lsidName="C. tomentosae" pageId="0" pageNumber="163" rank="species" species="tomentosae">
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<emphasis italics="true" pageId="0" pageNumber="163">C. tomentosae</emphasis>
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</taxonomicName>
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(5-15
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<normalizedToken originalValue="×">x</normalizedToken>
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18-24
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<normalizedToken originalValue="μm">μm</normalizedToken>
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vs. 18-24
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<normalizedToken originalValue="μm">μm</normalizedToken>
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).
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<taxonomicName authorityName="J. W. Liu, Manawas. & M. Luo" authorityYear="2023" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum tomentosae" order="Glomerellales" pageId="0" pageNumber="163" phylum="Ascomycota" rank="species" species="tomentosae">
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<emphasis italics="true" pageId="0" pageNumber="163">Colletotrichum tomentosae</emphasis>
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</taxonomicName>
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has longer conidiophores (20-40
|
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<normalizedToken originalValue="×">x</normalizedToken>
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3-5 vs. 12-16
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<normalizedToken originalValue="×">x</normalizedToken>
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4-5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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). This species can be distinguished from
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<taxonomicName lsidName="C. syzygicola" pageId="0" pageNumber="163" rank="species" species="syzygicola">
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<emphasis italics="true" pageId="0" pageNumber="163">C. syzygicola</emphasis>
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</taxonomicName>
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by 32 nucleotide differences (1/511 in the ITS region, 2/229 in the
|
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<emphasis italics="true" pageId="0" pageNumber="163">gapdh</emphasis>
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region, 7/242 in the
|
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<emphasis italics="true" pageId="0" pageNumber="163">act</emphasis>
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region and 22/906 in the
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<emphasis italics="true" pageId="0" pageNumber="163">gs</emphasis>
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region). The PHI test revealed no significant evidence for a recombination (p = 1.0) event amongst
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<taxonomicName lsidName="C. syzygicola" pageId="0" pageNumber="163" rank="species" species="syzygicola">
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<emphasis italics="true" pageId="0" pageNumber="163">C. syzygicola</emphasis>
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</taxonomicName>
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and its closely-related taxa (Fig.
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. PHI analysis of combined ITS, gapdh, chs- 1, act and β- tubulin sequence data. PHI test result (Φw) <0.05 indicates significant recombination within the dataset." figureDoi="10.3897/mycokeys.95.87121.figure3" httpUri="https://binary.pensoft.net/fig/814954" pageId="0" pageNumber="163">3</figureCitation>
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). Therefore, we have described this fungus as a novel species.
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</paragraph>
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<caption doi="10.3897/mycokeys.95.87121.figure3" httpUri="https://binary.pensoft.net/fig/814954" pageId="0" pageNumber="163" start="Figure 3" startId="F3">
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<paragraph pageId="0" pageNumber="163">
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<emphasis bold="true" pageId="0" pageNumber="163">Figure 3.</emphasis>
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PHI analysis of combined ITS,
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<emphasis italics="true" pageId="0" pageNumber="163">gapdh</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="163">chs-1</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="163">act</emphasis>
|
||
and
|
||
<normalizedToken originalValue="β-">β-</normalizedToken>
|
||
<emphasis italics="true" pageId="0" pageNumber="163">tubulin</emphasis>
|
||
sequence data. PHI test result (Φw) <0.05 indicates significant recombination within the dataset.
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |