236 lines
16 KiB
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236 lines
16 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.59852" ID-GBIF-Dataset="78bab002-8c3e-4548-8377-cb082fa11d13" ID-PMC="PMC7819953" ID-Pensoft-Pub="1314-4049-77-65" ID-Pensoft-UUID="5D8D5A6329A4584ABC11C425E4B0E47A" ID-PubMed="33519269" ModsDocID="1314-4049-77-65" checkinTime="1610662561303" checkinUser="pensoft" docAuthor="Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang" docDate="2021" docId="1A2EBCE23B375E62868651F5666ADB69" docLanguage="en" docName="MycoKeys 77: 65-95" docOrigin="MycoKeys 77" docPubDate="2021-01-14" docSource="http://dx.doi.org/10.3897/mycokeys.77.59852" docTitle="Diaporthe grandiflori S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li 2021, sp. nov." docType="treatment" docVersion="4" id="5D8D5A6329A4584ABC11C425E4B0E47A" lastPageNumber="65" masterDocId="5D8D5A6329A4584ABC11C425E4B0E47A" masterDocTitle="Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species" masterLastPageNumber="95" masterPageNumber="65" pageNumber="65" updateTime="1668136608950" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Li, Zhuang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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<mods:nameIdentifier type="email">junwuxue@126.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-01-14</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>77</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>65</mods:start>
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<mods:end>95</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.77.59852</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.59852</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-77-65</mods:identifier>
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<mods:identifier type="Pensoft-UUID">5D8D5A6329A4584ABC11C425E4B0E47A</mods:identifier>
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<treatment ID-GBIF-Taxon="176584082" LSID="urn:lsid:plazi:treatment:1A2EBCE23B375E62868651F5666ADB69" httpUri="http://treatment.plazi.org/id/1A2EBCE23B375E62868651F5666ADB69" lastPageNumber="65" pageId="0" pageNumber="65">
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<subSubSection pageId="0" pageNumber="65" type="nomenclature">
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName LSID="1A2EBCE2-3B37-5E62-8686-51F5666ADB69" authority="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe grandiflori" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="grandiflori" status="sp. nov.">Diaporthe grandiflori S.T. Huang, J.W. Xia, X.G. Zhang & Z. Li</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="65">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 3" captionStartId="F4" captionText="Figure 3. Diaporthe grandiflori (SAUCC 194.84) a leaf of Heterostemma grandiflorum b, c surface (b) and reverse (c) sides of colony after incubation for 15 days on PDA d conidiomata e conidiophores and conidiogenous cells f alpha conidia g, i alpha conidia and beta conidia h beta conidia. Scale bars: 10 μm (e-i)." figureDoi="10.3897/mycokeys.77.59852.figure3" httpUri="https://binary.pensoft.net/fig/497506" pageId="0" pageNumber="65">Figure 3</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="etymology">
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<paragraph pageId="0" pageNumber="65">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Named after the host
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<taxonomicName authorityName="Costantin" authorityYear="1912" class="Dicotyledoneae" family="Asclepiadaceae" genus="Heterostemma" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" lsidName="Heterostemma grandiflorum" order="Gentianales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="grandiflorum">
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<emphasis italics="true" pageId="0" pageNumber="65">Heterostemma grandiflorum</emphasis>
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</taxonomicName>
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on which it was collected.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="diagnosis">
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<paragraph pageId="0" pageNumber="65">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe grandiflori" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="grandiflori">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe grandiflori</emphasis>
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</taxonomicName>
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can be distinguished from the phylogenetically closely related species
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<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="65" rank="species" species="penetriteum">
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<emphasis italics="true" pageId="0" pageNumber="65">D. penetriteum</emphasis>
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</taxonomicName>
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Y.H. Gao & L. Cai in larger
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<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
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and
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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.
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</paragraph>
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<caption doi="10.3897/mycokeys.77.59852.figure3" httpUri="https://binary.pensoft.net/fig/497506" pageId="0" pageNumber="65" start="Figure 3" startId="F4">
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<paragraph pageId="0" pageNumber="65">
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<emphasis bold="true" pageId="0" pageNumber="65">Figure 3.</emphasis>
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe grandiflori" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="grandiflori">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe grandiflori</emphasis>
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</taxonomicName>
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(SAUCC194.84)
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<emphasis bold="true" pageId="0" pageNumber="65">a</emphasis>
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leaf of
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<taxonomicName authorityName="Costantin" authorityYear="1912" class="Dicotyledoneae" family="Asclepiadaceae" genus="Heterostemma" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" lsidName="Heterostemma grandiflorum" order="Gentianales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="grandiflorum">
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<emphasis italics="true" pageId="0" pageNumber="65">Heterostemma grandiflorum</emphasis>
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</taxonomicName>
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<emphasis bold="true" pageId="0" pageNumber="65">b, c</emphasis>
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surface (
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<emphasis bold="true" pageId="0" pageNumber="65">b</emphasis>
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) and reverse (
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<emphasis bold="true" pageId="0" pageNumber="65">c</emphasis>
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) sides of colony after incubation for 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">d</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="65">e</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="65">f</emphasis>
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alpha conidia
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<emphasis bold="true" pageId="0" pageNumber="65">g, i</emphasis>
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alpha conidia and beta conidia
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<emphasis bold="true" pageId="0" pageNumber="65">h</emphasis>
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beta conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="65">e-i</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="materials_examined">
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<paragraph pageId="0" pageNumber="65">Type.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on infected leaves of
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<taxonomicName authorityName="Costantin" authorityYear="1912" class="Dicotyledoneae" family="Asclepiadaceae" genus="Heterostemma" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" lsidName="Heterostemma grandiflorum" order="Gentianales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="grandiflorum">
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<emphasis italics="true" pageId="0" pageNumber="65">Heterostemma grandiflorum</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.84, holotype, ex-holotype living culture SAUCC194.84.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Description.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Asexual morph: Conidiomata pycnidial, subglobose to globose, solitary or aggregated in groups, black, erumpent, coated with white hyphae, thick-walled, exuding golden yellow spiral conidial cirrus from ostiole. Conidiophores hyaline, smooth, septate, branched, densely aggregated, cylindrical, straight to slightly sinuous, 9.5-16.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.9-2.8
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<normalizedToken originalValue="μm">μm</normalizedToken>
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. Conidiogenous cells 19.0-22.8
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4-2.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, cylindrical, multi-guttulate, terminal, tapering towards the apex. Alpha conidia abundant in culture, biguttulate, hyaline, smooth, aseptate, ellipsoidal, apex subobtuse, base subtruncate, 6.3-8.3
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<normalizedToken originalValue="×">x</normalizedToken>
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2.8-3.3
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 7.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.9
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Beta conidia, not numerous, hyaline, aseptate, filiform, slightly curved, tapering towards the apex, 21.5-30.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.1
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 24.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.7
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Gamma conidia not observed. Sexual morph not observed.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="65">Pure culture was isolated by subbing hyphal tips growing from surface sterilized plant material. Colonies on PDA cover the Petri dish after 15 days kept in dark conditions at 25 °C, cottony with abundant aerial mycelium, white on surface side, white to grayish on reverse.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="notes">
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<paragraph pageId="0" pageNumber="65">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Phylogenetic analysis of a combined five loci showed that
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<taxonomicName lsidName="D. grandiflori" pageId="0" pageNumber="65" rank="species" species="grandiflori">
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<emphasis italics="true" pageId="0" pageNumber="65">D. grandiflori</emphasis>
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</taxonomicName>
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(strain SAUCC194.84) formed an independent clade (Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Diaporthe based on combined ITS, TUB, TEF, CAL and HIS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Three branches were shortened to fit the page size - these are indicated by symbol (//) with indication number showing how many times they are shortened." figureDoi="10.3897/mycokeys.77.59852.figure1" httpUri="https://binary.pensoft.net/fig/497579" pageId="0" pageNumber="65">1</figureCitation>
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) and is phylogenetically distinct from
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<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="65" rank="species" species="penetriteum">
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<emphasis italics="true" pageId="0" pageNumber="65">D. penetriteum</emphasis>
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</taxonomicName>
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. This species can be easily distinguished from
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<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="65" rank="species" species="penetriteum">
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<emphasis italics="true" pageId="0" pageNumber="65">D. penetriteum</emphasis>
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</taxonomicName>
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by 87 nucleotides difference concatenated alignment (24 in the ITS region, 1
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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, 41
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and 21
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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). Morphologically,
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<taxonomicName lsidName="D. grandiflori" pageId="0" pageNumber="65" rank="species" species="grandiflori">
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<emphasis italics="true" pageId="0" pageNumber="65">D. grandiflori</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="65" rank="species" species="penetriteum">
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<emphasis italics="true" pageId="0" pageNumber="65">D. penetriteum</emphasis>
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</taxonomicName>
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in larger
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<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
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(6.3-8.3
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<normalizedToken originalValue="×">x</normalizedToken>
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2.8-3.3 vs. 4.5-5.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) and longer
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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(21.5-30.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.1 vs. 16.5-27.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-2.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) (
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<bibRefCitation DOI="https://doi.org/10.1080/14772000.2015.1101027" author="Gao, YH" journalOrPublisher="Systematics and Biodiversity" pageId="0" pageNumber="65" pagination="102 - 117" refId="B19" refString="Gao, YH, Liu, F, Cai, L, 2016. Unravelling Diaporthe species associated with Camellia. Systematics and Biodiversity 14 (1): 102 - 117, DOI: https://doi.org/10.1080/14772000.2015.1101027" title="Unravelling Diaporthe species associated with Camellia." url="https://doi.org/10.1080/14772000.2015.1101027" volume="14" year="2016">Gao et al. 2016</bibRefCitation>
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).
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |