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<document ID-CLB-Dataset="24772" ID-DOI="http://dx.doi.org/10.3897/mycokeys.60.39069" ID-GBIF-Dataset="ea7ac48b-6202-401d-86ea-19581c1264c9" ID-PMC="PMC6898192" ID-Pensoft-Pub="1314-4049-60-93" ID-Pensoft-UUID="7E7DFCA0FC455155AE9E1A3250242507" ID-PubMed="31824211" ModsDocID="1314-4049-60-93" checkinTime="1575106143164" checkinUser="pensoft" docAuthor="Yin, Mingliang, Wingfield, Michael J., Zhou, Xudong, Linnakoski, Riikka &amp; Beer, Z. Wilhelm de" docDate="2019" docId="0D08C846A29F5447AFD67AA0D062CE28" docLanguage="en" docName="MycoKeys 60: 93-123" docOrigin="MycoKeys 60" docSource="http://dx.doi.org/10.3897/mycokeys.60.39069" docTitle="Leptographium conplurium M. L. Yin, Z. W. de Beer &amp; M. J. Wingf., sp. nov." docType="treatment" docVersion="6" id="7E7DFCA0FC455155AE9E1A3250242507" lastPageNumber="93" masterDocId="7E7DFCA0FC455155AE9E1A3250242507" masterDocTitle="Taxonomy and phylogeny of the Leptographium olivaceum complex (Ophiostomatales, Ascomycota), including descriptions of six new species from China and Europe" masterLastPageNumber="123" masterPageNumber="93" pageNumber="93" updateTime="1732937562057" updateUser="ExternalLinkService">
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<mods:title id="204CBE71B05F6D602433EE4E777E664E">Taxonomy and phylogeny of the Leptographium olivaceum complex (Ophiostomatales, Ascomycota), including descriptions of six new species from China and Europe</mods:title>
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<mods:namePart id="226089F06E75EA29794185E94E85FDCB">Yin, Mingliang</mods:namePart>
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<mods:namePart id="3A6592E8EB3C51DA6271C10940212005">Wingfield, Michael J.</mods:namePart>
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<mods:namePart id="0FD91D9991C76E64FC6C04A959148441">Zhou, Xudong</mods:namePart>
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<mods:namePart id="BC2AA9CB53504DCB3236A67744C11AA7">Linnakoski, Riikka</mods:namePart>
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<mods:namePart id="3CBE7D11D1478B2E05D39936BEE19473">Beer, Z. Wilhelm de</mods:namePart>
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<treatment id="0D08C846A29F5447AFD67AA0D062CE28" ID-GBIF-Taxon="160607160" LSID="urn:lsid:plazi:treatment:0D08C846A29F5447AFD67AA0D062CE28" httpUri="http://treatment.plazi.org/id/0D08C846A29F5447AFD67AA0D062CE28" lastPageNumber="93" pageId="0" pageNumber="93" scope_order="Ophiostomatales" scope_phylum="Ascomycota">
<subSubSection id="1AF80A014EF80585C2D937C3845BC0B4" pageId="0" pageNumber="93" type="nomenclature">
<paragraph id="D54BD75AF4EC1F0AFE0F58FEF016EE22" pageId="0" pageNumber="93">
<taxonomicName id="6A7EB68CB9ED70DB4EF2206805C08DBC" ID-CoL="3TQBN" LSID="0D08C846-A29F-5447-AFD6-7AA0D062CE28" authority="M. L. Yin, Z. W. de Beer &amp; M. J. Wingf." class="Sordariomycetes" family="Ophiostomataceae" genus="Leptographium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Leptographium conplurium" order="Ophiostomatales" pageId="0" pageNumber="93" phylum="Ascomycota" rank="species" species="conplurium">Leptographium conplurium M.L. Yin, Z.W. de Beer &amp; M.J. Wingf.</taxonomicName>
<taxonomicNameLabel id="F91DA7FEF3F4BADA4A0A74CF653C4611" pageId="0" pageNumber="93">sp. nov.</taxonomicNameLabel>
<figureCitation id="2D4F0D8DF3809ABFA522FE968A3121B9" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Leptographium conplurium sp. nov. (CMW 23315). a fourteen-days old culture on OA with black background; b. synnematous asexual state on wood tissue on WA c conidiophore d conidiogenous apparatus e conidiogenous cells f conidia. Scale bars: 200 μm (b), 50 μm (c), 20 μm (d), 10 μm (e), 5 μm (f)." figureDoi="10.3897/mycokeys.60.39069.figure4" httpUri="https://binary.pensoft.net/fig/360783" pageId="0" pageNumber="93" tableDoi="10.3897/mycokeys.60.39069.figure4">Fig. 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="A2FF677820E4E2E4392F73F42FA4A684" pageId="0" pageNumber="93" type="etymology">
<paragraph id="CF9C2CB8559197D972D6CBC774435126" pageId="0" pageNumber="93">Etymology.</paragraph>
<paragraph id="EA3958C70F152E4398854555750A030B" pageId="0" pageNumber="93">The epithet refers to synnemata produced abundantly in culture.</paragraph>
</subSubSection>
<subSubSection id="725789B9D0B7B0B894A51A6C53E74748" pageId="0" pageNumber="93" type="materials_examined">
<paragraph id="546FB7CD2E8A3FA86CED17032A5427EC" pageId="0" pageNumber="93">Type.</paragraph>
<paragraph id="3C6A3D7AA0B8B5A29EACEC7307216243" pageId="0" pageNumber="93">
FINLAND, Ilomantsi, from
<taxonomicName id="96ED1B6566B0133D52E1E6DD970327A0" class="Pinopsida" family="Pinaceae" genus="Picea" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Picea abies" order="Pinales" pageId="0" pageNumber="93" phylum="Tracheophyta" rank="species" species="abies">
<emphasis id="CF55F757A8DE64A214F5EAC7F61356C1" italics="true" pageId="0" pageNumber="93">Picea abies</emphasis>
</taxonomicName>
infested with
<taxonomicName id="23C197BC86081546E543CAAA38A3548A" class="Insecta" family="Curculionidae" genus="Dryocoetes" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Dryocoetes autographus" order="Coleoptera" pageId="0" pageNumber="93" phylum="Arthropoda" rank="species" species="autographus">
<emphasis id="15D0827752D97A8841990CD559E7608F" italics="true" pageId="0" pageNumber="93">Dryocoetes autographus</emphasis>
</taxonomicName>
, Aug. 2005,
<emphasis id="D22BCA1D29F4A9C24BA62A43312AB228" italics="true" pageId="0" pageNumber="93">Z.W. de Beer</emphasis>
, (PREM 60918-
<emphasis id="B7D3C2E6F5D55CEB9FC2E1BD36B7B58D" bold="true" pageId="0" pageNumber="93">holotype</emphasis>
, ex-holotype cultures: CBS 128923 = CMW 23315); Ilomantsi, from
<taxonomicName id="B11757FC3650188633BA5CB8CC84F07A" lsidName="P. abies" pageId="0" pageNumber="93" rank="species" species="abies">
<emphasis id="30526E58AFD74D8EDF4D751BB3239E36" italics="true" pageId="0" pageNumber="93">P. abies</emphasis>
</taxonomicName>
infested with
<taxonomicName id="48EF65C3C2DA36CF2527DAF11BAB7A85" lsidName="D. autographus" pageId="0" pageNumber="93" rank="species" species="autographus">
<emphasis id="A73D18607EC34A6708B3814A1C951C2B" italics="true" pageId="0" pageNumber="93">D. autographus</emphasis>
</taxonomicName>
, Aug. 2005,
<emphasis id="5637452838292C0D5573B7F58AACE97D" italics="true" pageId="0" pageNumber="93">Z.W. de Bee</emphasis>
r, (PREM 60919-
<emphasis id="8254E1D0E197E59CEBF5C6AB8A1BEAFE" bold="true" pageId="0" pageNumber="93">paratype</emphasis>
, ex-paratype cultures: CBS 128834 = CMW 23289).
</paragraph>
</subSubSection>
<subSubSection id="927B418DE7E07D81AA3CCE10E6CDFE3C" pageId="0" pageNumber="93" type="description">
<paragraph id="E676A0CBB99E4DCB5FABC1D8BE07026D" pageId="0" pageNumber="93">Description.</paragraph>
<paragraph id="3A558435511AA4269CAE5D2CBBFCB61E" pageId="0" pageNumber="93">
<emphasis id="86D49020ED15442254ED69DC23D8BBD1" italics="true" pageId="0" pageNumber="93">Sexual state</emphasis>
not observed.
<emphasis id="C07D7288F89BB4AC19D7635F9D84FF68" italics="true" pageId="0" pageNumber="93">Conidiophores</emphasis>
macronematous, synnematous, 300-700
<normalizedToken id="7054E7F9C2DA5592DA9B4FE5CA131336" originalValue="μm">μm</normalizedToken>
including conidiogenous apparatus, synnemata occasionally swollen at the base, frequently swollen at the stipe, brown to black, expanding branches at the apex, (25
<normalizedToken id="2D656AE39B264692B98BF3C8AE1E7BB4" originalValue=")4050(">-)40-50(-</normalizedToken>
80)
<normalizedToken id="62D8B206A954F7A4249E2F8CC1D24E61" originalValue="μm">μm</normalizedToken>
in width, abundantly produced in culture.
<emphasis id="BE08251AED099598E5B84242DCE26579" italics="true" pageId="0" pageNumber="93">Conidiogenous cells</emphasis>
discrete, terminal, hyaline, cylindrical, (8
<normalizedToken id="70DC4EE7EC07E8CC49B6A8597C4E2307" originalValue=")1217(">-)12-17(-</normalizedToken>
20)
<normalizedToken id="43E7F0BDA447B14065A3ACA1A3599D74" originalValue="×">x</normalizedToken>
1.5-2.3
<normalizedToken id="B67EA558CD04C6DBED6C7E1C629E936D" originalValue="μm">μm</normalizedToken>
.
<emphasis id="47E62D2F74A934697CDB610F259F0BC1" italics="true" pageId="0" pageNumber="93">Conidia</emphasis>
hyaline, one-celled, ellipsoidal to cylindrical, (3.9
<normalizedToken id="82388B01304B931BD7C8978BC75969FE" originalValue=")4.34.9(">-)4.3-4.9(-</normalizedToken>
6.3)
<normalizedToken id="E9CDBF4A2F9B968338F8C537C604601C" originalValue="×">x</normalizedToken>
1.9-2.5
<normalizedToken id="7C103D724DE41A6F38150712CAF3D647" originalValue="μm">μm</normalizedToken>
.
<emphasis id="F408A7BAB79A99ADDF69D7BF45C64834" italics="true" pageId="0" pageNumber="93">Culture characteristics</emphasis>
: colonies on OA, hyaline at first, later becoming light yellowish in the center, concentric rings present, hyphae hyaline, appressed and immersed. Optimal growth temperature is 25 °C with radial growth rate 2.5 (
<normalizedToken id="FAADEEFAD78AD9717097B9EE7CCCA60C" originalValue="±">+/-</normalizedToken>
0.5) mm/d, growth reduced at 10 °C and 30 °C, no growth at 35 °C.
</paragraph>
<caption id="9EA8E46B14E94CC05878CAE3CB6CF9C2" doi="10.3897/mycokeys.60.39069.figure4" httpUri="https://binary.pensoft.net/fig/360783" pageId="0" pageNumber="93" start="Figure 4" startId="F4">
<paragraph id="FA37DF8B87E4529A240A5F48E0BF0277" pageId="0" pageNumber="93">
<emphasis id="299119EEE9973407B2856907C97A0761" bold="true" pageId="0" pageNumber="93">Figure 4.</emphasis>
<taxonomicName id="BAB26F9C007FB20C4B7D4099EA370769" class="Sordariomycetes" family="Ophiostomataceae" genus="Leptographium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Leptographium conplurium" order="Ophiostomatales" pageId="0" pageNumber="93" phylum="Ascomycota" rank="species" species="conplurium">
<emphasis id="A3ECFD033C09E541714B2A4C332F746D" italics="true" pageId="0" pageNumber="93">Leptographium conplurium</emphasis>
</taxonomicName>
sp. nov. (CMW 23315).
<emphasis id="AA3ABD100BD3E6398FF760A5384E5E91" bold="true" pageId="0" pageNumber="93">a</emphasis>
fourteen-days old culture on OA with black background; b. synnematous asexual state on wood tissue on WA
<emphasis id="11555EF2BFC25A2C88646310063E94A1" bold="true" pageId="0" pageNumber="93">c</emphasis>
conidiophore
<emphasis id="7ADB15D76CD5A95C6EBCF443D52469A9" bold="true" pageId="0" pageNumber="93">d</emphasis>
conidiogenous apparatus
<emphasis id="B10AA47F99F318B2722FE35B88277CE1" bold="true" pageId="0" pageNumber="93">e</emphasis>
conidiogenous cells
<emphasis id="06C439FAF9D81DEF9E4F5E8BD9D79847" bold="true" pageId="0" pageNumber="93">f</emphasis>
conidia. Scale bars: 200
<normalizedToken id="053D3C8FC085646A640B23E593999503" originalValue="μm">μm</normalizedToken>
(
<emphasis id="8579837D73B5D2728BB3BB0E6A8CA6EF" bold="true" pageId="0" pageNumber="93">b</emphasis>
), 50
<normalizedToken id="EA6ECB13679B4AD17D61B6C954332C2E" originalValue="μm">μm</normalizedToken>
(
<emphasis id="75E9D17592A06E2A6EC3B2A23EE0D746" bold="true" pageId="0" pageNumber="93">c</emphasis>
), 20
<normalizedToken id="E680CBF95AE61329145DB4AD92E96521" originalValue="μm">μm</normalizedToken>
(
<emphasis id="19B5399B963DE5E59E298BA4E55B54A1" bold="true" pageId="0" pageNumber="93">d</emphasis>
), 10
<normalizedToken id="71CF63A5B1BEF6536D1449F9D002C759" originalValue="μm">μm</normalizedToken>
(
<emphasis id="8CCD482B82594A589FAB8CD19187698A" bold="true" pageId="0" pageNumber="93">e</emphasis>
), 5
<normalizedToken id="3B94A19C7717027FBE967864EBC51BCB" originalValue="μm">μm</normalizedToken>
(
<emphasis id="CF0B4283D6430F81275F52473A08CA90" bold="true" pageId="0" pageNumber="93">f</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="E7AB35C00830F6493A7315592E7B3F9F" pageId="0" pageNumber="93" type="host">
<paragraph id="ED690C357BF7EC81D407BD41A921E021" pageId="0" pageNumber="93">Host tree.</paragraph>
<paragraph id="7F63ACB66FDDD9A9FFD49AC997A69C6A" pageId="0" pageNumber="93">
<taxonomicName id="9549C9CB9F1A143C555BACD71A299809" class="Pinopsida" family="Pinaceae" genus="Picea" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Picea abies" order="Pinales" pageId="0" pageNumber="93" phylum="Tracheophyta" rank="species" species="abies">
<emphasis id="E420B7284560F90E5B9105773594DD2E" italics="true" pageId="0" pageNumber="93">Picea abies</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection id="DB938A06B83288C188E5031599D4F8E5" pageId="0" pageNumber="93" type="insect vectors">
<paragraph id="299AC2AAD8F6831838D2A819955EE271" pageId="0" pageNumber="93">Insect vectors.</paragraph>
<paragraph id="EF2908F4D6C161C3A81030B1C4CD777D" pageId="0" pageNumber="93">
<taxonomicName id="249AF9BF9142021547A28AFFA9F90840" class="Insecta" family="Curculionidae" genus="Dryocoetes" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Dryocoetes autographus" order="Coleoptera" pageId="0" pageNumber="93" phylum="Arthropoda" rank="species" species="autographus">
<emphasis id="82894D46B3EECFE768C4E3593D615F3F" italics="true" pageId="0" pageNumber="93">Dryocoetes autographus</emphasis>
</taxonomicName>
,
<taxonomicName id="9366948CCD9EC33B63F56F32678E8343" class="Insecta" family="Curculionidae" genus="Hylastes" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Hylastes brunneus" order="Coleoptera" pageId="0" pageNumber="93" phylum="Arthropoda" rank="species" species="brunneus">
<emphasis id="AD599590492B332FA11A7825D235B595" italics="true" pageId="0" pageNumber="93">Hylastes brunneus</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection id="A4EDA05CB59EA85B4A540AF18DFD50CE" pageId="0" pageNumber="93" type="distribution">
<paragraph id="C2B822D111062EFC7F2AD579F73DD565" pageId="0" pageNumber="93">Distribution.</paragraph>
<paragraph id="4598FF54DD543054BBB64A7A5B0F7C41" pageId="0" pageNumber="93">Finland.</paragraph>
</subSubSection>
<subSubSection id="7E7E77DF3A89C9798EFDFB42AD134CD4" pageId="0" pageNumber="93" type="notes">
<paragraph id="C393AFC94BECE05C4112844BF7E330C6" pageId="0" pageNumber="93">Notes.</paragraph>
<paragraph id="4A7F42AF0F182F9BD5E5BA20329CEC96" pageId="0" pageNumber="93">
All isolates of this species were initially recognized as a cryptic species closely related to
<taxonomicName id="7B4F9C991544DB0908747DDA69BC4D9E" lsidName="L. cucullatum" pageId="0" pageNumber="93" rank="species" species="cucullatum">
<emphasis id="B9F35018EA0104BF381E4CCDC00E59DE" italics="true" pageId="0" pageNumber="93">L. cucullatum</emphasis>
</taxonomicName>
and
<taxonomicName id="BF4CAB42673A283602DE8AD0DDD87B3D" lsidName="L. olivaceapini" pageId="0" pageNumber="93" rank="species" species="olivaceapini">
<emphasis id="ED82C8DBBE8437B98E2D753122D0AA17" italics="true" pageId="0" pageNumber="93">L. olivaceapini</emphasis>
</taxonomicName>
by
<bibRefCitation id="8E303EF1CD52BF9086EB7FCEB00FF4D8" DOI="https://doi.org/10.1007/s10482-012-9747-6" author="Linnakoski, R" journalOrPublisher="Antonie van Leeuwenhoek" pageId="0" pageNumber="93" pagination="375 - 399" refId="B26" refString="Linnakoski, R, De Beer, ZW, Duong, TA, Niemelae, P, Pappinen, A, et, al., 2012. Grosmannia and Leptographium spp. associated with conifer-infesting bark beetles in Finland and Russia, including Leptographiumtaigense sp. nov. Antonie van Leeuwenhoek 102: 375 - 399" title="Grosmannia and Leptographium spp. associated with conifer-infesting bark beetles in Finland and Russia, including Leptographiumtaigense sp. nov." url="https://doi.org/10.1007/s10482-012-9747-6" volume="102" year="2012">Linnakoski et al. (2012)</bibRefCitation>
. Our results confirmed that they represent an undescribed taxon.
</paragraph>
</subSubSection>
<subSubSection id="170175817918B9A22C4D000E6B6E2B86" pageId="0" pageNumber="93" type="materials_examined">
<paragraph id="DDB4CD79004EE94FAF2E3F917E1609CF" pageId="0" pageNumber="93">Additional material examined.</paragraph>
<paragraph id="8012DE2227C23E0A06B09F72E616F824" pageId="0" pageNumber="93">
FINLAND, Ilomantsi, from
<taxonomicName id="B0A4ED19A7D756611F57C34B22128630" class="Pinopsida" family="Pinaceae" genus="Picea" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Picea abies" order="Pinales" pageId="0" pageNumber="93" phylum="Tracheophyta" rank="species" species="abies">
<emphasis id="FE90586DB7CB2714E97D97F579068B75" italics="true" pageId="0" pageNumber="93">Picea abies</emphasis>
</taxonomicName>
infested with
<taxonomicName id="812C8BF8E06CE757BFD159430B337C51" class="Insecta" family="Curculionidae" genus="Dryocoetes" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Dryocoetes autographus" order="Coleoptera" pageId="0" pageNumber="93" phylum="Arthropoda" rank="species" species="autographus">
<emphasis id="D9B68BF5E6A7AEB722A34314E88159EB" italics="true" pageId="0" pageNumber="93">Dryocoetes autographus</emphasis>
</taxonomicName>
, Aug. 2005,
<emphasis id="6C16718E9F5DA6B0312BACAB84E28120" italics="true" pageId="0" pageNumber="93">Z.W. de Beer</emphasis>
, (culture: CMW 23295); Ilomantsi, from
<taxonomicName id="F170483FB60440479818EFC6E0E211A2" lsidName="P. abies" pageId="0" pageNumber="93" rank="species" species="abies">
<emphasis id="3E692D4D386C33A17C88FDE36360A8E3" italics="true" pageId="0" pageNumber="93">P. abies</emphasis>
</taxonomicName>
infested with
<taxonomicName id="5A773B94869DC020599DABB81AC66186" class="Insecta" family="Curculionidae" genus="Hylastes" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Hylastes brunneus" order="Coleoptera" pageId="0" pageNumber="93" phylum="Arthropoda" rank="species" species="brunneus">
<emphasis id="6E21D79A23BD75F0F5FCBD7E40969A46" italics="true" pageId="0" pageNumber="93">Hylastes brunneus</emphasis>
</taxonomicName>
, Aug. 2005,
<emphasis id="0A2AF7C2C7394AEF8A190F01258704C2" italics="true" pageId="0" pageNumber="93">Z.W. de Beer</emphasis>
, (culture: CMW 23316).
</paragraph>
</subSubSection>
</treatment>
</document>