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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.80.63816" ID-GBIF-Dataset="0e25cb67-367d-4bdf-a0b3-b27e66746dc6" ID-PMC="PMC8149378" ID-Pensoft-Pub="1314-4049-80-77" ID-Pensoft-UUID="32CB43C6B50A508687C832DAC17CFF21" ID-PubMed="34054325" ModsDocID="1314-4049-80-77" checkinTime="1621365476539" checkinUser="pensoft" docAuthor="Wang, Xianhong, Guo, Yashuang, Du, Yamin, Yang, Ziling, Huang, Xinzhong, Hong, Ni, Xu, Wenxing &amp; Wang, Guoping" docDate="2021" docId="0AD93BED536853758F266C5AC411C956" docLanguage="en" docName="MycoKeys 80: 77-90" docOrigin="MycoKeys 80" docPubDate="2021-05-18" docSource="http://dx.doi.org/10.3897/mycokeys.80.63816" docTitle="Diaporthe zaofenghuang X. H. Wang &amp; G. P. Wang 2021, sp. nov." docType="treatment" docVersion="4" id="32CB43C6B50A508687C832DAC17CFF21" lastPageNumber="77" masterDocId="32CB43C6B50A508687C832DAC17CFF21" masterDocTitle="Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China" masterLastPageNumber="90" masterPageNumber="77" pageNumber="77" updateTime="1668136662822" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Xianhong</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-7810-9274</mods:nameIdentifier>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Guo, Yashuang</mods:namePart>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Du, Yamin</mods:namePart>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Ziling</mods:namePart>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Huang, Xinzhong</mods:namePart>
<mods:affiliation>Research Institute of Pomology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hong, Ni</mods:namePart>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China &amp; Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, Hubei, 430070, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Xu, Wenxing</mods:namePart>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China &amp; Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, Hubei, 430070, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Guoping</mods:namePart>
<mods:affiliation>Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China &amp; Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, Hubei, 430070, China</mods:affiliation>
<mods:nameIdentifier type="email">gpwang@mail.hzau.edu.cn</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-05-18</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>80</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>77</mods:start>
<mods:end>90</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.80.63816</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.80.63816</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-80-77</mods:identifier>
<mods:identifier type="Pensoft-UUID">32CB43C6B50A508687C832DAC17CFF21</mods:identifier>
</mods:mods>
<subSection pageId="0" pageNumber="77" type="taxonomy">
<treatment ID-GBIF-Taxon="182223098" LSID="urn:lsid:plazi:treatment:0AD93BED536853758F266C5AC411C956" httpUri="http://treatment.plazi.org/id/0AD93BED536853758F266C5AC411C956" lastPageNumber="77" pageId="0" pageNumber="77">
<subSubSection pageId="0" pageNumber="77" type="nomenclature">
<paragraph pageId="0" pageNumber="77">
<taxonomicName LSID="0AD93BED-5368-5375-8F26-6C5AC411C956" authority="X. H. Wang &amp; G. P. Wang" authorityName="X. H. Wang &amp; G. P. Wang" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe zaofenghuang" order="Diaporthales" pageId="0" pageNumber="77" phylum="Ascomycota" rank="species" species="zaofenghuang" status="sp. nov.">Diaporthe zaofenghuang X.H. Wang &amp; G.P. Wang</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="77">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Diaporthe zaofenghuang. Front and back views of colonies on PDA (A, B) and OA (C, D), respectively E conidiomata on alfalfa stems F conidioma G conidiophores H alpha conidia I-J section view of conidioma. Scale bars: 2 mm (E); 200 μm (F); 20 μm (G, I); 10 μm (H, J)." figureDoi="10.3897/mycokeys.80.63816.figure4" httpUri="https://binary.pensoft.net/fig/544343" pageId="0" pageNumber="77">Fig. 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="77" type="etymology">
<paragraph pageId="0" pageNumber="77">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="77">
Named after the host species (
<taxonomicName class="Dicotyledoneae" family="Rosaceae" genus="Prunus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Prunus persica" order="Rosales" pageId="0" pageNumber="77" phylum="Angiospermae" rank="species" species="persica">
<emphasis italics="true" pageId="0" pageNumber="77">Prunus persica</emphasis>
</taxonomicName>
cv. Zaofenghuang) from which the species was isolated.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="77" type="description">
<paragraph pageId="0" pageNumber="77">Description.</paragraph>
<paragraph pageId="0" pageNumber="77">
Sexual morph not observed. Asexual morph on alfalfa stems.
<emphasis italics="true" pageId="0" pageNumber="77">Pycnidial conidiomata</emphasis>
conical, yellowish translucent conidial drops exuded from ostioles, 650-1430
<normalizedToken originalValue="μm">μm</normalizedToken>
diam.
<emphasis italics="true" pageId="0" pageNumber="77">Conidiophores</emphasis>
fasciculate, hyaline, long cylindrical, straight or slightly curved, apex pointed, 13.7-20.9
<normalizedToken originalValue="×">x</normalizedToken>
1.8-2.7
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<emphasis italics="true" pageId="0" pageNumber="77">Conidiogenous cells</emphasis>
phialidic, cylindrical.
<emphasis italics="true" pageId="0" pageNumber="77">Alpha conidia</emphasis>
hyaline, aseptate, ellipsoidal, biguttulate, rounded at one end, slightly apex at another end, 5.3-7.5
<normalizedToken originalValue="×">x</normalizedToken>
2.9-3.7
<normalizedToken originalValue="µm">µm</normalizedToken>
(mean = 6.0
<normalizedToken originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken originalValue="×">x</normalizedToken>
3.1
<normalizedToken originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 50).
<emphasis italics="true" pageId="0" pageNumber="77">Beta</emphasis>
and
<emphasis italics="true" pageId="0" pageNumber="77">gamma conidia</emphasis>
not observed.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="77" type="description">
<paragraph pageId="0" pageNumber="77">Culture characteristics.</paragraph>
<paragraph pageId="0" pageNumber="77">Cultures incubated on PDA at 25 °C in cycle of light/darkness, growth rate 8.5 mm per day. Colonies initially white on surface, producing black pigment from center of medium and expanding outwardly after 5-7 d. On PDA, edge of colony petal-like, irregular; on OA, edge relatively flat.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="77" type="materials_examined">
<paragraph pageId="0" pageNumber="77">Materials examined.</paragraph>
<paragraph pageId="0" pageNumber="77">
<materialsCitation collectingDate="2017-03-23" collectorName="Y. S. Guo" country="China" location="Zaofenghuang" municipality="Sanming City" specimenCount="1" stateProvince="Fujian Province" typeStatus="holotype">
<collectingCountry name="China">China</collectingCountry>
,
<collectingRegion country="China" name="Fujian">Fujian Province</collectingRegion>
,
<collectingMunicipality>Sanming City</collectingMunicipality>
, on buds of
<taxonomicName class="Dicotyledoneae" family="Rosaceae" genus="Prunus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Prunus persica" order="Rosales" pageId="0" pageNumber="77" phylum="Angiospermae" rank="species" species="persica">
<emphasis italics="true" pageId="0" pageNumber="77">Prunus persica</emphasis>
</taxonomicName>
cv.
<location LSID="urn:lsid:plazi:treatment:0AD93BED536853758F266C5AC411C956:EFCB16041F6583BD50E47ECD036CAF78" country="China" municipality="Sanming City" name="Zaofenghuang" stateProvince="Fujian Province">Zaofenghuang</location>
,
<collectingDate value="2017-03-23">23 March 2017</collectingDate>
,
<collectorName>Y. S. Guo</collectorName>
(
<typeStatus>holotype</typeStatus>
HMAS 249835, culture ex-holotype CGMCC3.20271 = TZFH1)
</materialsCitation>
;
<materialsCitation collectingDate="2021-01-01" collectingDateMax="2021-12-31" collectingDateMin="2021-01-01" collectorName="Y. S. Guo" country="China" location="Zaofenghuang" municipality="Sanming City" specimenCount="1" stateProvince="Fujian Province" typeStatus="holotype">ibid., culture TZFH3</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="77" type="notes">
<paragraph pageId="0" pageNumber="77">Notes.</paragraph>
<paragraph pageId="0" pageNumber="77">
Two isolates representing
<taxonomicName lsidName="D. zaofenghuang" pageId="0" pageNumber="77" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">D. zaofenghuang</emphasis>
</taxonomicName>
form a well-supported clade (1/100/100) and appear to be most closely related to
<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="77" rank="species" species="penetriteum">
<emphasis italics="true" pageId="0" pageNumber="77">D. penetriteum</emphasis>
</taxonomicName>
.
<taxonomicName authorityName="X. H. Wang &amp; G. P. Wang" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe zaofenghuang" order="Diaporthales" pageId="0" pageNumber="77" phylum="Ascomycota" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">Diaporthe zaofenghuang</emphasis>
</taxonomicName>
can be distinguished from
<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="77" rank="species" species="penetriteum">
<emphasis italics="true" pageId="0" pageNumber="77">D. penetriteum</emphasis>
</taxonomicName>
based on ITS,
<emphasis italics="true" pageId="0" pageNumber="77">HIS</emphasis>
, and
<emphasis italics="true" pageId="0" pageNumber="77">TUB</emphasis>
loci (10/578 in ITS, 45/520 in
<emphasis italics="true" pageId="0" pageNumber="77">HIS</emphasis>
, and 7/525 in
<emphasis italics="true" pageId="0" pageNumber="77">TUB</emphasis>
). Morphologically,
<taxonomicName lsidName="D. zaofenghuang" pageId="0" pageNumber="77" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">D. zaofenghuang</emphasis>
</taxonomicName>
differs from
<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="77" rank="species" species="penetriteum">
<emphasis italics="true" pageId="0" pageNumber="77">D. penetriteum</emphasis>
</taxonomicName>
in having larger conidiomata (
<taxonomicName lsidName="D. zaofenghuang" pageId="0" pageNumber="77" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">D. zaofenghuang</emphasis>
</taxonomicName>
= 653-1433
<normalizedToken originalValue="μm">μm</normalizedToken>
vs
<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="77" rank="species" species="penetriteum">
<emphasis italics="true" pageId="0" pageNumber="77">D. penetriteum</emphasis>
</taxonomicName>
= 180-490
<normalizedToken originalValue="μm">μm</normalizedToken>
) and
<emphasis italics="true" pageId="0" pageNumber="77">alpha</emphasis>
conidia (
<taxonomicName lsidName="D. zaofenghuang" pageId="0" pageNumber="77" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">D. zaofenghuang</emphasis>
</taxonomicName>
= 6.0
<normalizedToken originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken originalValue="×">x</normalizedToken>
3.1
<normalizedToken originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken originalValue="μm">μm</normalizedToken>
vs
<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="77" rank="species" species="penetriteum">
<emphasis italics="true" pageId="0" pageNumber="77">D. penetriteum</emphasis>
</taxonomicName>
= 5.0
<normalizedToken originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken originalValue="×">x</normalizedToken>
2.2
<normalizedToken originalValue="±">+/-</normalizedToken>
0.2
<normalizedToken originalValue="μm">μm</normalizedToken>
). Additionally,
<taxonomicName lsidName="D. penetriteum" pageId="0" pageNumber="77" rank="species" species="penetriteum">
<emphasis italics="true" pageId="0" pageNumber="77">D. penetriteum</emphasis>
</taxonomicName>
produces two types of conidia, but
<taxonomicName lsidName="D. zaofenghuang" pageId="0" pageNumber="77" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">D. zaofenghuang</emphasis>
</taxonomicName>
produces only
<emphasis italics="true" pageId="0" pageNumber="77">alpha</emphasis>
conidia (
<bibRefCitation DOI="https://doi.org/10.1080/14772000.2015.1101027" author="Gao, YH" journalOrPublisher="Worre-Mertens, Luxemburg" pageId="0" pageNumber="77" refId="B12" refString="Gao, YH, Fang Liu, F, Cai, L, 2016. Unravelling Diaporthe species associated with Camellia, Systematics and Biodiversity 14: 102-117. https://doi.org/10.1080/14772000.2015.1101027" title="Unravelling Diaporthe species associated with Camellia, Systematics and Biodiversity 14: 102 - 117." url="https://doi.org/10.1080/14772000.2015.1101027" year="2016">Gao et al. 2016</bibRefCitation>
).
</paragraph>
<caption doi="10.3897/mycokeys.80.63816.figure4" httpUri="https://binary.pensoft.net/fig/544343" pageId="0" pageNumber="77" start="Figure 4" startId="F4">
<paragraph pageId="0" pageNumber="77">
<emphasis bold="true" pageId="0" pageNumber="77">Figure 4.</emphasis>
<taxonomicName authorityName="X. H. Wang &amp; G. P. Wang" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe zaofenghuang" order="Diaporthales" pageId="0" pageNumber="77" phylum="Ascomycota" rank="species" species="zaofenghuang">
<emphasis italics="true" pageId="0" pageNumber="77">Diaporthe zaofenghuang</emphasis>
</taxonomicName>
. Front and back views of colonies on PDA (
<emphasis bold="true" pageId="0" pageNumber="77">A, B</emphasis>
) and OA (
<emphasis bold="true" pageId="0" pageNumber="77">C, D</emphasis>
), respectively
<emphasis bold="true" pageId="0" pageNumber="77">E</emphasis>
conidiomata on alfalfa stems
<emphasis bold="true" pageId="0" pageNumber="77">F</emphasis>
conidioma
<emphasis bold="true" pageId="0" pageNumber="77">G</emphasis>
conidiophores
<emphasis bold="true" pageId="0" pageNumber="77">H</emphasis>
<emphasis italics="true" pageId="0" pageNumber="77">alpha</emphasis>
conidia
<emphasis bold="true" pageId="0" pageNumber="77">I-J</emphasis>
section view of conidioma. Scale bars: 2 mm (
<emphasis bold="true" pageId="0" pageNumber="77">E</emphasis>
); 200
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="77">F</emphasis>
); 20
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="77">G, I</emphasis>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="77">H, J</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</subSection>
</document>