141 lines
15 KiB
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141 lines
15 KiB
XML
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<mods:title id="E7E811B6A5184E42304B76E57C880897">Eupholides A H, abietane diterpenoids from the roots of Euphorbia fischeriana, and their bioactivities</mods:title>
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<mods:namePart id="54184BCF527C0E1DACB1B248FB94C5A0">Li, Da-Wei</mods:namePart>
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<mods:namePart id="78218D96134C1AFF1398AF0D3919423A">Deng, Xiao-Peng</mods:namePart>
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<mods:namePart id="2AC2AB0F71EC68B59E7530C35F807035">He, Xin</mods:namePart>
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<mods:namePart id="6A360F1C65E53C3FB8443F40DF697593">Feng, Lei</mods:namePart>
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<mods:title id="6AB1C57AA0273BF75D312E1423306D4F">Phytochemistry</mods:title>
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<mods:date id="F1B1CAE0CC8A1F5BCFA2679AA0C59A62">2021</mods:date>
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<treatment id="03F57F05FFEFFF920A2783C6FDFAFB6E" LSID="urn:lsid:plazi:treatment:03F57F05FFEFFF920A2783C6FDFAFB6E" httpUri="http://treatment.plazi.org/id/03F57F05FFEFFF920A2783C6FDFAFB6E" lastPageNumber="7" pageId="6" pageNumber="7">
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<subSubSection id="C3469D98FFEFFF920A2783C6FD9EFC56" box="[100,581,957,977]" pageId="6" pageNumber="7" type="nomenclature">
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<paragraph id="8BE3CE13FFEFFF920A2783C6FD9EFC56" blockId="6.[100,581,957,977]" box="[100,581,957,977]" pageId="6" pageNumber="7">
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<emphasis id="B9281201FFEFFF920A2783C6FD9EFC56" bold="true" box="[100,581,957,977]" italics="true" pageId="6" pageNumber="7">
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4.6. Inhibitory effects on
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<taxonomicName id="4C5CB590FFEFFF920B0D83C5FD9EFC56" ID-CoL="44W74" ID-ENA="1310115" authority="(Zopf, 1883) Lehmann & Neumann, 1896" authorityName="(Zopf, 1883) Lehmann & Neumann" box="[334,581,957,976]" class="Actinobacteridae" family="Mycobacteriaceae" genus="Mycobacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="tuberculosis">Mycobacterium tuberculosis</taxonomicName>
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<paragraph id="8BE3CE13FFEFFF920AC7838DFDFAFB6E" blockId="6.[100,770,1013,1256]" pageId="6" pageNumber="7">
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The inhibitory effects of isolated diterpenoids against
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<taxonomicName id="4C5CB590FFEFFF92083F838EFCDAFB8E" authority="(Zopf, 1883) Lehmann & Neumann, 1896" box="[636,769,1013,1033]" class="Actinobacteridae" family="Mycobacteriaceae" genus="Mycobacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="tuberculosis">
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<emphasis id="B9281201FFEFFF92083F838EFCDAFB8E" bold="true" box="[636,769,1013,1033]" italics="true" pageId="6" pageNumber="7">M. tuberculosis</emphasis>
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were investigated using an Alamar blue cell viability assay (Thermo Fisher Scientific Inc.) in 96-well microplates. The test materials were dissolved in DMSO at an initial concentration of 50 mM. Ethambutol was used as the positive control antimicrobial agent.
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<taxonomicName id="4C5CB590FFEFFF92086C841DFD6EFBFE" authority="(Zopf, 1883) Lehmann & Neumann, 1896" box="[559,693,1125,1144]" class="Actinobacteridae" family="Mycobacteriaceae" genus="Mycobacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="tuberculosis">
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<emphasis id="B9281201FFEFFF92086C841DFD6EFBFE" bold="true" box="[559,693,1125,1144]" italics="true" pageId="6" pageNumber="7">M. tuberculosis</emphasis>
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and the tested diterpenoids were coincubated in culture medium at 37
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<superScript id="7C29635BFFEFFF9208E88406FD69FB0D" attach="right" box="[683,690,1150,1163]" fontSize="6" pageId="6" pageNumber="7">◦</superScript>
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C. Then, the bacterial cells were determined by absorbance measurement at
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<quantity id="4CA463F6FFEFFF92089D84E5FF5EFB4A" metricMagnitude="-7" metricUnit="m" metricValue="5.95" pageId="6" pageNumber="7" unit="nm" value="595.0">595 nm</quantity>
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. The detailed procedure about
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<taxonomicName id="4C5CB590FFEFFF920BF284C1FDE3FB4D" authority="(Zopf, 1883) Lehmann & Neumann, 1896" box="[433,568,1208,1228]" class="Actinobacteridae" family="Mycobacteriaceae" genus="Mycobacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="tuberculosis">
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<emphasis id="B9281201FFEFFF920BF284C1FDE3FB4D" bold="true" box="[433,568,1208,1228]" italics="true" pageId="6" pageNumber="7">M. tuberculosis</emphasis>
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inhibitory assay was performed as previous work (
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<bibRefCitation id="EFCDB3E2FFEFFF920B3884ADFDCFFB6E" author="Han, X. Y. & Chen, C. M. & Yan, Q. L. & Jia, L. Q. & Taj, A. & Ma, Y. F." box="[379,532,1236,1256]" journalOrPublisher="Front. Microbiol." pageId="6" pageNumber="7" pagination="1799" part="10" refId="ref9426" refString="Han, X. Y., Chen, C. M., Yan, Q. L., Jia, L. Q., Taj, A., Ma, Y. F., 2019. Action of dicumarol on glucosamine- 1 - phosphate acetyltransferase of GlmU and Mycobacterium tuberculosis. Front. Microbiol. 10, 1799." title="Action of dicumarol on glucosamine- 1 - phosphate acetyltransferase of GlmU and Mycobacterium tuberculosis" type="journal article" year="2019">Han et al., 2019</bibRefCitation>
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).
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</paragraph>
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</treatment>
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