810 lines
87 KiB
XML
810 lines
87 KiB
XML
<document id="B6F50D15E033C196986E36F11973845D" ID-DOI="10.1016/j.ijppaw.2024.100963" ID-ISSN="2213-2244" ID-Zenodo-Dep="13289449" IM.bibliography_approvedBy="guilherme" IM.illustrations_approvedBy="guilherme" IM.materialsCitations_approvedBy="guilherme" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="guilherme" checkinTime="1723245211737" checkinUser="felipe" docAuthor="Shock, Barbara C., Jones, Håkon H., Garrett, Kayla B., Hernandez, Sonia M., Burchfield, Holly J., Haman, Katie, Schwantje, Helen, Telford, Sam R., Cunningham, Mark W. & Yabsley, Michael J." docDate="2024" docId="03CD87F4FB3CAA67FFA9FADEAAF1624D" docLanguage="en" docName="IntJParasitolParasitesWildlife.24.100963.pdf" docOrigin="International Journal for Parasitology: Parasites and Wildlife 24 (4)" docSource="http://dx.doi.org/10.1016/j.ijppaw.2024.100963" docStyle="DocumentStyle:77CC2CF2FBA7BCD129DD16D0D4F96931.2:IntJParasitolParasitesWildlife.2012-.journal_article" docStyleId="77CC2CF2FBA7BCD129DD16D0D4F96931" docStyleName="IntJParasitolParasitesWildlife.2012-.journal_article" docStyleVersion="2" docTitle="Babesia coryicola Shock, Jones, Garrett, Hernandez, Burchfield, Haman, Schwantje, Telford, Cunningham & Yabsley, 2024, sp. nov." docType="treatment" docVersion="1" masterDocId="FFF4FF8CFB3EAA62FFCDFFD3AB30641C" masterDocTitle="Description of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) from southern Florida, USA" masterLastPageNumber="123153" masterPageNumber="123153" pageNumber="123153" updateTime="1727959619804" updateUser="guilherme" zenodo-license-document="CC-BY-NC-ND-4.0">
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<mods:titleInfo id="F35E66DD054A5BC4E20B697D80A0EC7E">
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<mods:title id="C6FE1AEA0095D13E102064A49EE12D91">Description of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) from southern Florida, USA</mods:title>
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<mods:namePart id="48FF7950F9F1FB05E36F5AADD840E4A2">Shock, Barbara C.</mods:namePart>
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<mods:affiliation id="4CD87391B80AAFE58DD3CEA731D67110">Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA & Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA</mods:affiliation>
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<mods:namePart id="6BB9DC08537B75F27A19A82B75AC2352">Jones, Håkon H.</mods:namePart>
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<mods:affiliation id="126278F5FD4B4BFC3586CAA62282AFBE">Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA</mods:affiliation>
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<mods:roleTerm id="3FF4BBBA1E4A40BA58175A9E3EDFB64D">Author</mods:roleTerm>
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<mods:namePart id="1231EB8088FB4B96EC3C4E088454B817">Garrett, Kayla B.</mods:namePart>
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<mods:affiliation id="5952E04FCB6A684FDB6DAF1AE8801E06">Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA & Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA</mods:affiliation>
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<mods:namePart id="4BC934EAEC7D04E7D5B973A347665A12">Hernandez, Sonia M.</mods:namePart>
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<mods:affiliation id="3A83D2E55E35175CDC99513807367633">Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA & Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA</mods:affiliation>
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</mods:role>
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<mods:namePart id="FF694F62B06C40701321794D60926059">Burchfield, Holly J.</mods:namePart>
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<mods:affiliation id="7F06C92185E3B9D49B11FE93C572E1DB">Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA</mods:affiliation>
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<mods:namePart id="6F5CA4C262C5A25B7E4E55481DFC452C">Haman, Katie</mods:namePart>
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<mods:affiliation id="AF62339E9202F1D09DD5FF1063EEEA28">Wildlife Program, Washington Department of Fish and Wildlife, 1111 Washington Street SE, Olympia, WA, 98504, USA</mods:affiliation>
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</mods:name>
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<mods:namePart id="92A98A12240B770F9AB26D462C18E470">Schwantje, Helen</mods:namePart>
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<mods:affiliation id="FED49E5510F41F3C5C259C765A8C899B">British Columbia Ministry of Forests, Lands and Natural Resource Operations, Nanaimo, British Columbia, Canada</mods:affiliation>
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</mods:name>
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<mods:namePart id="4EB254CB445805FFEDB5FAB170528910">Telford, Sam R.</mods:namePart>
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<mods:affiliation id="FA77AF684D7DD144E1B3763A548F8532">Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, USA</mods:affiliation>
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</mods:name>
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<mods:name id="A86ECEC012C1B1345DF8C0027F866D27" type="personal">
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</mods:role>
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<mods:namePart id="FE016F4BBD02094A05BE714821B18829">Cunningham, Mark W.</mods:namePart>
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<mods:affiliation id="4E9A202C883AB7B847843949BC200611">Florida Fish and Wildlife Conservation Commission, Gainesville, FL, 32601, USA</mods:affiliation>
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</mods:name>
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</mods:role>
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<mods:namePart id="3B96CC4D31B67E5420B850F36CD19C1C">Yabsley, Michael J.</mods:namePart>
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<mods:affiliation id="86BC947666FC06B473B1ACF0A5E5080F">Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA & Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA & Center for Ecology of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA</mods:affiliation>
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<mods:typeOfResource id="973278D6305EE49E742D1AB8E84E516E">text</mods:typeOfResource>
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<mods:title id="9C0E7E31BFF80A75F94144D010F66CF1">International Journal for Parasitology: Parasites and Wildlife</mods:title>
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</mods:titleInfo>
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<mods:part id="66E50154F858EE08B04F6728D57694C7">
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<mods:date id="23CF766509BD857455E3FB834DB1FA0C">2024</mods:date>
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<mods:number id="18BCC713EE68DFE8C485FBF47B25D39B">2024-08-31</mods:number>
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<mods:number id="5F50299EF5FCBD22702363E563F9EE16">24</mods:number>
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<mods:number id="69C98C0935E47D5F31B9BE30A3C05F53">4</mods:number>
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<mods:url id="E0C0DFBD54DE8689624CF5CFA0E87B58">http://dx.doi.org/10.1016/j.ijppaw.2024.100963</mods:url>
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<mods:identifier id="F6B7B08AB63E111B1AC62302324FB217" type="DOI">10.1016/j.ijppaw.2024.100963</mods:identifier>
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<mods:identifier id="B18B4447CBDEC4D729BF3303AFCA1E31" type="ISSN">2213-2244</mods:identifier>
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<mods:identifier id="9CA2656ACD424F82A3FC7692B0D753CF" type="Zenodo-Dep">13289449</mods:identifier>
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</mods:mods>
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<treatment id="03CD87F4FB3CAA67FFA9FADEAAF1624D" LSID="urn:lsid:plazi:treatment:03CD87F4FB3CAA67FFA9FADEAAF1624D" httpUri="http://treatment.plazi.org/id/03CD87F4FB3CAA67FFA9FADEAAF1624D" lastPageId="5" pageId="2">
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<subSubSection id="C37E6569FB3CAA60FFA9FADEAB956120" pageId="2" type="nomenclature">
|
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<paragraph id="8BDB36E2FB3CAA60FFA9FADEAB956120" blockId="2.[100,740,1293,1340]" pageId="2">
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<emphasis id="B910EAF0FB3CAA60FFA9FADEAB956120" bold="true" italics="true" pageId="2">
|
||
3.1. Description of
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||
<taxonomicName id="4C644D61FB3CAA60FED5FADEAA84613C" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[280,436,1293,1312]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">Babesia coryicola</taxonomicName>
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||
<taxonomicNameLabel id="A223578BFB3CAA60FE76FADEA930613C" box="[443,512,1293,1312]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
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||
Shock, cunningham, and yabsley
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</emphasis>
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||
</paragraph>
|
||
</subSubSection>
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||
<subSubSection id="C37E6569FB3CAA60FF49FAB2AA12618C" pageId="2" type="biology_ecology">
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||
<paragraph id="8BDB36E2FB3CAA60FF49FAB2AA12618C" blockId="2.[100,771,1376,1982]" pageId="2">
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||
<typeStatus id="54DF8840FB3CAA60FF49FAB2AB826168" box="[132,178,1377,1396]" pageId="2">Type</typeStatus>
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||
host:
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||
<location id="8EBB6039FB3CAA60FF31FAB2AA706168" LSID="urn:lsid:plazi:treatment:03CD87F4FB3CAA67FFA9FADEAAF1624D:8EBB6039FB3CAA60FF31FAB2AA706168" box="[252,320,1377,1396]" name="Female" pageId="2" stateProvince="Florida">Female</location>
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||
<collectingRegion id="49A0F800FB3CAA60FE83FAB2AAA16168" box="[334,401,1377,1396]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
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||
puma (
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||
<emphasis id="B910EAF0FB3CAA60FE24FAB2A9806168" bold="true" box="[489,688,1376,1396]" italics="true" pageId="2">
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||
Puma
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||
<taxonomicName id="4C644D61FB3CAA60FDE7FAB3A946616F" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[554,630,1376,1395]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="2" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
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coryi
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</emphasis>
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) FP222 (
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<taxonomicName id="4C644D61FB3CAA60FFA7FAAEABE2618C" authorityName="Linnaeus" authorityYear="1758" box="[106,210,1405,1424]" class="Mammalia" kingdom="Animalia" pageId="2" phylum="Chordata" rank="class">Mammalia</taxonomicName>
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||
:
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<taxonomicName id="4C644D61FB3CAA60FF18FAAEAA2C618C" authorityName="Fischer de Waldheim" authorityYear="1817" box="[213,284,1405,1424]" class="Mammalia" family="Felidae" kingdom="Animalia" order="Carnivora" pageId="2" phylum="Chordata" rank="family">Felidae</taxonomicName>
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)
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C37E6569FB3CAA60FF49FA4AA94A61E3" pageId="2" type="materials_examined">
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||
<paragraph id="8BDB36E2FB3CAA60FF49FA4AAADA61B0" blockId="2.[100,771,1376,1982]" box="[132,490,1433,1452]" pageId="2">
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||
<typeStatus id="54DF8840FB3CAA60FF49FA4AAB8261B0" box="[132,178,1433,1452]" pageId="2">Type</typeStatus>
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locality:
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<collectingCounty id="62BA4E6EFB3CAA60FEC6FA4AAAA661B0" box="[267,406,1433,1452]" pageId="2">Collier County</collectingCounty>
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||
,
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<collectingRegion id="49A0F800FB3CAA60FE6DFA4AAAD661B0" box="[416,486,1433,1452]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
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||
.
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</paragraph>
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<paragraph id="8BDB36E2FB3CAA60FF49FA66A94A61E3" blockId="2.[100,771,1376,1982]" pageId="2">
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||
<materialsCitation id="3B0C3CBFFB3CAA60FF49FA66A94661E3" collectionCode="USNPC" collectorName="Hapantotype. A" country="United States of America" county="Collier County" location="Collier County" pageId="2" specimenCount="1" stateProvince="Florida" typeStatus="hapantotype">
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||
Type material:
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||
<typeStatus id="54DF8840FB3CAA60FED8FA66AAA161D4" box="[277,401,1461,1480]" pageId="2">Hapantotype</typeStatus>
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||
. A thin-stained blood smear from
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||
<collectingRegion id="49A0F800FB3CAA60FD18FA67ABB161FF" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
puma FP222 from
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||
<collectingCounty id="62BA4E6EFB3CAA60FEFAFA03AAF261FF" box="[311,450,1488,1507]" pageId="2">Collier County</collectingCounty>
|
||
, Florida was deposited in the U.S. National Parasite Collection (
|
||
<collectionCode id="ED75AE27FB3CAA60FEB5FA3FAA8E61E3" box="[376,446,1516,1535]" collectionName="USNPC" pageId="2">USNPC</collectionCode>
|
||
accession number)
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||
</materialsCitation>
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||
.
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||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C37E6569FB3CAA67FF49F9DBAAF1624D" lastPageId="5" pageId="2" type="description">
|
||
<paragraph id="8BDB36E2FB3CAA60FF49F9DBAA67624F" blockId="2.[100,771,1376,1982]" pageId="2">
|
||
Parahapantotype. A thin-stained blood smear from
|
||
<collectingRegion id="49A0F800FB3CAA60FD4DF9DBA9F36207" box="[640,707,1544,1563]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
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||
puma FP93 was deposited in the
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<collectingCountry id="F3737672FB3CAA60FEBCF9F7AAA8622B" box="[369,408,1572,1591]" name="United States of America" pageId="2">U.S.</collectingCountry>
|
||
National Parasite Collection (USNPC accession number xxxxx
|
||
<emphasis id="B910EAF0FB3CAA60FE85F993AA67624F" bold="true" box="[328,343,1600,1619]" italics="true" pageId="2">).</emphasis>
|
||
</paragraph>
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||
<paragraph id="8BDB36E2FB3CAA60FF49F98FAABD6273" blockId="2.[100,771,1376,1982]" box="[132,397,1628,1647]" pageId="2">Vector: Currently unknown.</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FF49F9ABAA6962C3" blockId="2.[100,771,1376,1982]" pageId="2">Representative sequences: Genbank DQ329139 and PP852299 (18S rRNA gene); KJ592628 (ITS1 region); KJ592629 (ITS2 region); DQ329139 (β- tubulin gene); PP855655 (COI gene); PP855654 (cytb gene); PP855655 (COX3).</paragraph>
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||
<paragraph id="8BDB36E2FB3CAA60FF49F934A87B667D" blockId="2.[100,771,1376,1982]" lastBlockId="2.[818,1487,590,944]" pageId="2">
|
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Etymology:
|
||
<taxonomicName id="4C644D61FB3CAA60FF3BF934AAA362E6" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[246,403,1767,1786]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3CAA60FF3BF934AAA362E6" bold="true" box="[246,403,1767,1786]" italics="true" pageId="2">Babesia coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3CAA60FE57F93BAAD562E7" box="[410,485,1768,1787]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
|
||
is named after the subspecies name of the only known host, the endangered
|
||
<collectingRegion id="49A0F800FB3CAA60FDE0F8D0A940630A" box="[557,624,1795,1814]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
puma
|
||
<emphasis id="B910EAF0FB3CAA60FD7BF8D0ABA0632E" bold="true" italics="true" pageId="2">
|
||
<taxonomicName id="4C644D61FB3CAA60FD7BF8D0A832630A" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[694,770,1795,1814]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="2" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
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coryi
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||
</emphasis>
|
||
. When the
|
||
<collectingRegion id="49A0F800FB3CAA60FF37F8CCAA0D632E" box="[250,317,1823,1842]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
puma was initially described in 1896 by Charles B. Cory it was named
|
||
<emphasis id="B910EAF0FB3CAA60FEF6F8E8AA8B6352" bold="true" box="[315,443,1851,1870]" italics="true" pageId="2">
|
||
<taxonomicName id="4C644D61FB3CAA60FEF6F8E8AA526352" box="[315,354,1851,1870]" family="Theileriidae" genus="Cytauxzoon" higherTaxonomySource="CoL" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="felis">Felis</taxonomicName>
|
||
<taxonomicName id="4C644D61FB3CAA60FEA7F8E8AA8B6352" box="[362,443,1851,1870]" pageId="2" rank="species" species="floridana">floridana</taxonomicName>
|
||
</emphasis>
|
||
and was later renamed
|
||
<emphasis id="B910EAF0FB3CAA60FD6BF8E8A8326352" bold="true" box="[678,770,1851,1870]" italics="true" pageId="2">
|
||
<taxonomicName id="4C644D61FB3CAA60FD6BF8E8A9FD6352" box="[678,717,1851,1870]" family="Theileriidae" genus="Cytauxzoon" higherTaxonomySource="CoL" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="felis">Felis</taxonomicName>
|
||
coryi
|
||
</emphasis>
|
||
because
|
||
<taxonomicName id="4C644D61FB3CAA60FF75F884AA136375" box="[184,291,1878,1898]" pageId="2" rank="species" species="floridana">
|
||
<emphasis id="B910EAF0FB3CAA60FF75F884AA136375" bold="true" box="[184,291,1878,1898]" italics="true" pageId="2">F. floridana</emphasis>
|
||
</taxonomicName>
|
||
was previously used for the bobcat (
|
||
<emphasis id="B910EAF0FB3CAA60FD58F885A8336376" bold="true" box="[661,771,1878,1898]" italics="true" pageId="2">
|
||
<taxonomicName id="4C644D61FB3CAA60FD58F885A98C6375" box="[661,700,1878,1897]" family="Theileriidae" genus="Cytauxzoon" higherTaxonomySource="CoL" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="felis">Felis</taxonomicName>
|
||
<taxonomicName id="4C644D61FB3CAA60FD0BF884A9C86376" baseAuthorityName="Schreber" baseAuthorityYear="1777" box="[710,760,1879,1898]" class="Mammalia" family="Felidae" genus="Lynx" kingdom="Animalia" order="Carnivora" pageId="2" phylum="Chordata" rank="species" species="rufus">rufus</taxonomicName>
|
||
).
|
||
</emphasis>
|
||
Following the taxonomic revision of pumas by
|
||
<bibRefCitation id="EFF54B13FB3CAA60FDFBF8A0AB9463BE" author="Nelson, E. W. & Goldman, E. A." pageId="2" pagination="345 - 350" refId="ref14553" refString="Nelson, E. W., Goldman, E. A., 1929. List of the pumas with three described as new. J. Mammal. 10, 345 - 350." type="journal article" year="1929">
|
||
<collectingRegion id="49A0F800FB3CAA60FDFBF8A0A946639A" box="[566,630,1907,1926]" country="New Zealand" name="Nelson" pageId="2">Nelson</collectingRegion>
|
||
and Goldman (1929)
|
||
</bibRefCitation>
|
||
, the
|
||
<collectingRegion id="49A0F800FB3CAA60FF2CF85CAA1463BE" box="[225,292,1935,1954]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
puma was reclassified as a subspecies. Name: coryi-refers to the subspecies of the
|
||
<collectingRegion id="49A0F800FB3CAA60FE7CF878AAC463A2" box="[433,500,1963,1982]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
puma and -cola is inhabitant of.
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FC9CFDB9AF766685" blockId="2.[818,1487,590,944]" pageId="2">
|
||
ZooBank reference:
|
||
<uri id="FFF53AE0FB3CAA60FBF4FDB9AF726685" pageId="2">
|
||
urn:lsid:zoobank.org:pub:
|
||
<uuid id="FFC20C37FB3CAA60FAEAFDB9AF726685" pageId="2">F7CD2D01-3B51- 4AAC-A30B-6D2240A07C17</uuid>
|
||
</uri>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FC9CFD71A84D67AC" blockId="2.[818,1487,590,944]" pageId="2">
|
||
Description: All parasites observed (n = 18) were ring stage trophozoites which were morphologically similar to small
|
||
<taxonomicName id="4C644D61FB3CAA60FA98FD6DAEFC66CD" box="[1365,1484,702,721]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3CAA60FA98FD6DAEA966CD" bold="true" box="[1365,1433,702,721]" italics="true" pageId="2">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
, including felid-associated species such as
|
||
<taxonomicName id="4C644D61FB3CAA60FB79FD09AFD666F0" baseAuthorityName="Linnaeus" baseAuthorityYear="1758" box="[1204,1254,729,749]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="2" phylum="Chordata" rank="species" species="leo">
|
||
<emphasis id="B910EAF0FB3CAA60FB79FD09AFD666F0" bold="true" box="[1204,1254,729,749]" italics="true" pageId="2">B. leo</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C644D61FB3CAA60FADFFD09AE7D66F0" box="[1298,1357,729,749]" family="Theileriidae" genus="Cytauxzoon" higherTaxonomySource="CoL" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="felis">
|
||
<emphasis id="B910EAF0FB3CAA60FADFFD09AE7D66F0" bold="true" box="[1298,1357,729,749]" italics="true" pageId="2">B. felis</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="135F2A67FB3CAA60FA97FD09AEBE66F1" box="[1370,1422,730,749]" captionStart="Fig" captionStartId="3.[100,130,746,763]" captionTargetBox="[107,1484,149,718]" captionTargetId="figure-13@3.[106,1485,148,719]" captionTargetPageId="3" captionText="Fig. 1. Photomicrographs of Babesia coryicola sp. nov., type-material in blood smears from FP222 Florida puma (Puma concolor coryi) (A–C) showing ring and amoeboid trophozoites and FP93 (D) showing a compact ring form." figureDoi="http://doi.org/10.5281/zenodo.13289451" httpUri="https://zenodo.org/record/13289451/files/figure.png" pageId="2">Fig. 1</figureCitation>
|
||
). Most trophozoites were compact ring forms with small amounts of cytoplasm and a single nucleus, but rare trophozoites with 2–4 nuclei were observed. The overall shape of most trophozoites were round, but rare oval or slightly amoeboid forms were observed. Trophozoites measured 0.85–1.3 μm (0.991 μm average) in length and 0.75–1.2 μm (0.941 μm average) in width with no tetrad (Maltese cross) shapes observed (
|
||
<figureCitation id="135F2A67FB3CAA60FCF7FC4EA85F67AC" box="[826,879,925,944]" captionStart="Fig" captionStartId="3.[100,130,746,763]" captionTargetBox="[107,1484,149,718]" captionTargetId="figure-13@3.[106,1485,148,719]" captionTargetPageId="3" captionText="Fig. 1. Photomicrographs of Babesia coryicola sp. nov., type-material in blood smears from FP222 Florida puma (Puma concolor coryi) (A–C) showing ring and amoeboid trophozoites and FP93 (D) showing a compact ring form." figureDoi="http://doi.org/10.5281/zenodo.13289451" httpUri="https://zenodo.org/record/13289451/files/figure.png" pageId="2">Fig. 1</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FCFFFC04AE3D67F5" blockId="2.[818,1293,982,1002]" box="[818,1293,982,1002]" pageId="2">
|
||
<heading id="D093818EFB3CAA60FCFFFC04AE3D67F5" box="[818,1293,982,1002]" fontSize="36" level="2" pageId="2" reason="3">
|
||
<emphasis id="B910EAF0FB3CAA60FCFFFC04AE3D67F5" bold="true" box="[818,1293,982,1002]" italics="true" pageId="2">3.2. Natural history studies on host(s) and locations</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FC9CFBDDAE4E61E0" blockId="2.[818,1488,1038,1532]" pageId="2">
|
||
The parasite has been detected in blood samples collected from Florida pumas sampled from 1989 to 2013. A previous survey of Florida pumas reported a prevalence of 95% (37 of 39 pumas) (
|
||
<bibRefCitation id="EFF54B13FB3CAA60FA87FB95A8526069" author="Yabsley, M. J. & Murphy, S. M. & Cunningham, Andm W." pageId="2" pagination="366 - 374" refId="ref16705" refString="Yabsley, M. J., Murphy, S. M., Cunningham, Andm W., 2006. Molecular detection and characterization of Cytauxzoon felis and a Babesia species in cougars from Florida. J. Wildl. Dis. 42, 366 - 374." type="journal article" year="2006">Yabsley et al., 2006</bibRefCitation>
|
||
). In the current study, 72% of 100 Florida pumas were PCR positive for
|
||
<taxonomicName id="4C644D61FB3CAA60FC4AFBADA8C6608D" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[903,1014,1150,1169]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3CAA60FC4AFBADA8C6608D" bold="true" box="[903,1014,1150,1169]" italics="true" pageId="2">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3CAA60FBCCFBADAF61608D" box="[1025,1105,1150,1169]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
|
||
To date, only Florida pumas in their southern Florida (Collier, Hendry, Broward, Monroe, and Dale Counties) range have been infected. Five cougars from Texas that were translocated to Florida were negative for
|
||
<taxonomicName id="4C644D61FB3CAA60FB5BFB01AE3360F8" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1174,1283,1233,1253]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3CAA60FB5BFB01AE3360F8" bold="true" box="[1174,1283,1233,1253]" italics="true" pageId="2">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3CAA60FAC6FB01AE6760F9" box="[1291,1367,1234,1253]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
|
||
before their release and two of these animals resampled after release remained negative (
|
||
<bibRefCitation id="EFF54B13FB3CAA60FC5CFAD9AF646101" author="Rotstein, D. S. & Taylor, S. K. & Harvey, J. W. & Bean, J." box="[913,1108,1290,1309]" pageId="2" pagination="613 - 617" refId="ref15245" refString="Rotstein, D. S., Taylor, S. K., Harvey, J. W., Bean, J., 1999. Hematologic effects of cytauxzoonosis in Florida panthers and Texas cougars in Florida. J. Wildl. Dis. 35, 613 - 617." type="journal article" year="1999">Rotstein et al., 1999</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFF54B13FB3CAA60FBACFAD9AE2F6101" author="Yabsley, M. J. & Murphy, S. M. & Cunningham, Andm W." box="[1121,1311,1290,1309]" pageId="2" pagination="366 - 374" refId="ref16705" refString="Yabsley, M. J., Murphy, S. M., Cunningham, Andm W., 2006. Molecular detection and characterization of Cytauxzoon felis and a Babesia species in cougars from Florida. J. Wildl. Dis. 42, 366 - 374." type="journal article" year="2006">Yabsley et al., 2006</bibRefCitation>
|
||
). Additionally, 19 pumas from Texas, introduced into northern Florida in 1993 and removed in 1995 for a reintroduction feasibility study, were also blood-smear negative for piroplasms prior to their introduction to Florida (Belden and McCown, 1996;
|
||
<bibRefCitation id="EFF54B13FB3CAA60FB5CFAAAAE626190" author="Rotstein, D. S. & Taylor, S. K. & Harvey, J. W. & Bean, J." box="[1169,1362,1401,1421]" pageId="2" pagination="613 - 617" refId="ref15245" refString="Rotstein, D. S., Taylor, S. K., Harvey, J. W., Bean, J., 1999. Hematologic effects of cytauxzoonosis in Florida panthers and Texas cougars in Florida. J. Wildl. Dis. 35, 613 - 617." type="journal article" year="1999">Rotstein et al., 1999</bibRefCitation>
|
||
). Our results confirm that these pumas were negative for piroplasms; by PCR however, some of the pumas did acquire
|
||
<taxonomicName id="4C644D61FB3CAA60FB5FFA62AFFF61D8" box="[1170,1231,1457,1476]" family="Theileriidae" genus="Cytauxzoon" higherTaxonomySource="CoL" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="felis">
|
||
<emphasis id="B910EAF0FB3CAA60FB5FFA62AFFF61D8" bold="true" box="[1170,1231,1457,1476]" italics="true" pageId="2">C. felis</emphasis>
|
||
</taxonomicName>
|
||
infections while in Florida (
|
||
<bibRefCitation id="EFF54B13FB3CAA60FCF7FA1EA8C761FC" author="Rotstein, D. S. & Taylor, S. K. & Harvey, J. W. & Bean, J." box="[826,1015,1485,1504]" pageId="2" pagination="613 - 617" refId="ref15245" refString="Rotstein, D. S., Taylor, S. K., Harvey, J. W., Bean, J., 1999. Hematologic effects of cytauxzoonosis in Florida panthers and Texas cougars in Florida. J. Wildl. Dis. 35, 613 - 617." type="journal article" year="1999">Rotstein et al., 1999</bibRefCitation>
|
||
). All pumas tested from Georgia, Louisiana, North Dakota,
|
||
<collectingCountry id="F3737672FB3CAA60FC4CFA3AA8F961E0" box="[897,969,1513,1532]" name="Canada" pageId="2">Canada</collectingCountry>
|
||
, and
|
||
<collectingCountry id="F3737672FB3CAA60FC31FA3AAF6F61E0" box="[1020,1119,1513,1532]" name="Costa Rica" pageId="2">Costa Rica</collectingCountry>
|
||
were negative for piroplasms.
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FCFFF9F1AF076229" blockId="2.[818,1079,1570,1589]" box="[818,1079,1570,1589]" pageId="2">
|
||
<heading id="D093818EFB3CAA60FCFFF9F1AF076229" box="[818,1079,1570,1589]" fontSize="36" level="2" pageId="2" reason="3">
|
||
<emphasis id="B910EAF0FB3CAA60FCFFF9F1AF076229" bold="true" box="[818,1079,1570,1589]" italics="true" pageId="2">3.3. Serologic characteristics</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FC9CF989AFF862C1" blockId="2.[818,1487,1626,1757]" pageId="2">
|
||
Samples from two
|
||
<taxonomicName id="4C644D61FB3CAA60FC31F989AF546271" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1020,1124,1626,1645]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3CAA60FC31F989AF546271" bold="true" box="[1020,1124,1626,1645]" italics="true" pageId="2">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3CAA60FBA5F989AF816271" box="[1128,1201,1626,1645]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
|
||
<emphasis id="B910EAF0FB3CAA60FB62F989AF866271" bold="true" box="[1199,1206,1626,1645]" italics="true" pageId="2">-</emphasis>
|
||
positive
|
||
<collectingRegion id="49A0F800FB3CAA60FAC9F989AE776271" box="[1284,1351,1626,1645]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
puma samples displayed fluorescence at a 1:128 dilution for
|
||
<taxonomicName id="4C644D61FB3CAA60FB26F9A5AE026295" box="[1259,1330,1654,1673]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="bovis">
|
||
<emphasis id="B910EAF0FB3CAA60FB26F9A5AE026295" bold="true" box="[1259,1330,1654,1673]" italics="true" pageId="2">B. bovis</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C644D61FB3CAA60FAAEF9A5AE9A6295" baseAuthorityName="Piana & Galli-Valerio" baseAuthorityYear="1895" box="[1379,1450,1654,1673]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="canis">
|
||
<emphasis id="B910EAF0FB3CAA60FAAEF9A5AE9A6295" bold="true" box="[1379,1450,1654,1673]" italics="true" pageId="2">B. canis</emphasis>
|
||
</taxonomicName>
|
||
antigens. Samples from four
|
||
<taxonomicName id="4C644D61FB3CAA60FBE2F941AFAB62B8" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1071,1179,1681,1701]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3CAA60FBE2F941AFAB62B8" bold="true" box="[1071,1179,1681,1701]" italics="true" pageId="2">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3CAA60FB6EF941AFDF62B9" box="[1187,1263,1682,1701]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
|
||
positive
|
||
<collectingRegion id="49A0F800FB3CAA60FA8AF941AEBA62B9" box="[1351,1418,1682,1701]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
pumas displayed fluorescence at 1:256 dilution for
|
||
<taxonomicName id="4C644D61FB3CAA60FB2EF97DAE7E62DC" box="[1251,1358,1709,1729]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="odocoilei">
|
||
<emphasis id="B910EAF0FB3CAA60FB2EF97DAE7E62DC" bold="true" box="[1251,1358,1709,1729]" italics="true" pageId="2">B. odocoilei</emphasis>
|
||
</taxonomicName>
|
||
antigens. No sample displayed cross-reaction for
|
||
<taxonomicName id="4C644D61FB3CAA60FB49F919AFF162C1" box="[1156,1217,1738,1757]" family="Theileriidae" genus="Theileria" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="equi">
|
||
<emphasis id="B910EAF0FB3CAA60FB49F919AFF162C1" bold="true" box="[1156,1217,1738,1757]" italics="true" pageId="2">T. equi</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FCFFF8D0AF3A630A" blockId="2.[818,1034,1795,1814]" box="[818,1034,1795,1814]" pageId="2">
|
||
<heading id="D093818EFB3CAA60FCFFF8D0AF3A630A" box="[818,1034,1795,1814]" fontSize="36" level="2" pageId="2" reason="3">
|
||
<emphasis id="B910EAF0FB3CAA60FCFFF8D0AF3A630A" bold="true" box="[818,1034,1795,1814]" italics="true" pageId="2">3.4. Molecular analysis</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA60FCFFF8E8AFA26352" blockId="2.[818,1488,1851,1982]" box="[818,1170,1851,1870]" pageId="2">
|
||
<heading id="D093818EFB3CAA60FCFFF8E8AFA26352" box="[818,1170,1851,1870]" fontSize="36" level="2" pageId="2" reason="8">
|
||
<emphasis id="B910EAF0FB3CAA60FCFFF8E8AFA26352" bold="true" box="[818,1170,1851,1870]" italics="true" pageId="2">3.4.1. Near full-length 18S rRNA gene</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3CAA66FC9CF884A9C86552" blockId="2.[818,1488,1851,1982]" lastBlockId="4.[100,771,148,334]" lastPageId="4" pageId="2">
|
||
The near full-length 18S rRNA gene sequences of
|
||
<taxonomicName id="4C644D61FB3CAA60FAD7F884AEB26375" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1306,1410,1878,1898]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3CAA60FAD7F884AEB26375" bold="true" box="[1306,1410,1878,1898]" italics="true" pageId="2">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3CAA60FA4AF884AEE06376" box="[1415,1488,1879,1898]" pageId="2" rank="species">sp. nov.</taxonomicNameLabel>
|
||
were obtained from three
|
||
<collectingRegion id="49A0F800FB3CAA60FBF0F8A0AFB0639A" box="[1085,1152,1907,1926]" country="United States of America" name="Florida" pageId="2">Florida</collectingRegion>
|
||
pumas (FP40, FP44, FP222). Sequences from FP40 and FP44 were identical and were 99.9% similar to FL222 (1608/1,610bp). These sequences were most similar to a
|
||
<taxonomicName id="4C644D61FB3CAA60FA46F879AEFF63A1" box="[1419,1487,1962,1981]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="2" phylum="Miozoa" rank="genus">
|
||
<emphasis id="B910EAF0FB3CAA60FA46F879AEFF63A1" bold="true" box="[1419,1487,1962,1981]" italics="true" pageId="2">Babesia</emphasis>
|
||
</taxonomicName>
|
||
sp. reported from a tick removed from a dog in
|
||
<collectingCountry id="F3737672FB3AAA66FDDAFF47A97F64BB" box="[535,591,148,167]" name="Japan" pageId="4">Japan</collectingCountry>
|
||
(
|
||
<collectingRegion id="49A0F800FB3AAA66FD94FF47A9BF64BB" box="[601,655,148,167]" country="Japan" name="Akita" pageId="4">Akita</collectingRegion>
|
||
610) (98.3% (1651/1679), AY191024) and a
|
||
<taxonomicName id="4C644D61FB3AAA66FE5DFF7CAAC464DF" box="[400,500,175,195]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FE5DFF7CAAE464DE" bold="true" box="[400,468,175,194]" italics="true" pageId="4">Babesia</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
from a red panda from
|
||
<collectingCountry id="F3737672FB3AAA66FD06FF63A83264DF" box="[715,770,176,195]" name="China" pageId="4">China</collectingCountry>
|
||
(98.1% (1691/1724), OK524313) (Supplementary File 1 shows distance matrix with other
|
||
<taxonomicName id="4C644D61FB3AAA66FED9FF34AABA64E7" box="[276,394,231,251]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FED9FF34AA6864E6" bold="true" box="[276,344,231,250]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
). Phylogenetically,
|
||
<taxonomicName id="4C644D61FB3AAA66FD88FF3BA98064E6" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[581,688,231,251]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FD88FF3BA98064E6" bold="true" box="[581,688,231,251]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FD7AFF3BA83364E7" box="[695,771,232,251]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
grouped with other
|
||
<taxonomicName id="4C644D61FB3AAA66FEE9FED0AA58650A" box="[292,360,259,278]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="genus" sensu="stricto">
|
||
<emphasis id="B910EAF0FB3AAA66FEE9FED0AA58650A" bold="true" box="[292,360,259,278]" italics="true" pageId="4">Babesia</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FEBDFED7AAD5650A" box="[368,485,259,279]" pageId="4" sensu="stricto">sensu stricto</taxonomicNameLabel>
|
||
species as an ancestral group within a large group of
|
||
<taxonomicName id="4C644D61FB3AAA66FE9CFECCAAF5652F" box="[337,453,287,307]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FE9CFECCAAA5652E" bold="true" box="[337,405,287,306]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
of ungulates (
|
||
<figureCitation id="135F2A67FB3AAA66FD9EFECCA9BB652E" box="[595,651,287,306]" captionStart="Fig" captionStartId="3.[100,130,1862,1879]" captionTargetBox="[340,1247,928,1832]" captionTargetId="figure-54@3.[338,1248,926,1834]" captionTargetPageId="3" captionText="Fig. 2. Genetic relationships of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) compared with other Babesia spp. based on near full length 18S rRNA gene sequences. The text in bold blue in the figure represents specimens analyzed in this study. Sequences in light blue are species that have been primarily associated with felid hosts. Green lineages are predominately associated with canid hosts but have been reported in felids. Several sequences derived from domestic cats (i.e., MW578972, PP151898, and PP151899) and wild felids (i.e., HQ187782 and HQ187782) were not included in the analysis because the sequences were short." figureDoi="http://doi.org/10.5281/zenodo.13289453" httpUri="https://zenodo.org/record/13289453/files/figure.png" pageId="4">Fig. 2</figureCitation>
|
||
). This large group was a sister group to a clade of carnivore-infecting
|
||
<taxonomicName id="4C644D61FB3AAA66FD4BFEE8A9C86552" box="[646,760,315,334]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FD4BFEE8A9FA6552" bold="true" box="[646,714,315,334]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
</paragraph>
|
||
<caption id="DF1B666AFB3DAA61FFA9FD39A9996709" ID-DOI="http://doi.org/10.5281/zenodo.13289451" ID-Zenodo-Dep="13289451" httpUri="https://zenodo.org/record/13289451/files/figure.png" pageId="3" startId="3.[100,130,746,763]" targetBox="[107,1484,149,718]" targetPageId="3" targetType="figure">
|
||
<paragraph id="8BDB36E2FB3DAA61FFA9FD39A9996709" blockId="3.[100,1487,746,789]" pageId="3">
|
||
<emphasis id="B910EAF0FB3DAA61FFA9FD39ABAF66E7" bold="true" box="[100,159,746,763]" pageId="3">Fig. 1.</emphasis>
|
||
Photomicrographs of
|
||
<taxonomicName id="4C644D61FB3DAA61FEA9FD39AAC266E7" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[356,498,746,763]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="3" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3DAA61FEA9FD39AAC266E7" bold="true" box="[356,498,746,763]" italics="true" pageId="3">Babesia coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3DAA61FE37FD38A97166E7" box="[506,577,747,763]" pageId="3" rank="species">sp. nov.</taxonomicNameLabel>
|
||
, type-material in blood smears from FP222 Florida puma (
|
||
<emphasis id="B910EAF0FB3DAA61FB8BFD38AFC066E7" bold="true" box="[1094,1264,746,763]" italics="true" pageId="3">
|
||
Puma
|
||
<taxonomicName id="4C644D61FB3DAA61FBB1FD39AFF066E7" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[1148,1216,746,763]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="3" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
|
||
coryi
|
||
</emphasis>
|
||
) (A–C) showing ring and amoeboid trophozoites and FP93 (D) showing a compact ring form.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF1B666AFB3DAA61FFA9F895ABF663A1" ID-DOI="http://doi.org/10.5281/zenodo.13289453" ID-Zenodo-Dep="13289453" httpUri="https://zenodo.org/record/13289453/files/figure.png" pageId="3" startId="3.[100,130,1862,1879]" targetBox="[340,1247,928,1832]" targetPageId="3" targetType="figure">
|
||
<paragraph id="8BDB36E2FB3DAA61FFA9F895ABF663A1" blockId="3.[100,1487,1862,1981]" pageId="3">
|
||
<emphasis id="B910EAF0FB3DAA61FFA9F895ABAD634B" bold="true" box="[100,157,1862,1879]" pageId="3">Fig. 2.</emphasis>
|
||
Genetic relationships of
|
||
<taxonomicName id="4C644D61FB3DAA61FEB8F895A931634B" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[373,513,1862,1879]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="3" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3DAA61FEB8F895A931634B" bold="true" box="[373,513,1862,1879]" italics="true" pageId="3">Babesia coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3DAA61FDCBF894A97A634B" box="[518,586,1863,1879]" pageId="3" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from Florida pumas (
|
||
<emphasis id="B910EAF0FB3DAA61FCCFF894A897634B" bold="true" box="[770,935,1862,1879]" italics="true" pageId="3">
|
||
Puma
|
||
<taxonomicName id="4C644D61FB3DAA61FCFBF895A84A634B" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[822,890,1862,1879]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="3" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
|
||
coryi
|
||
</emphasis>
|
||
) compared with other
|
||
<taxonomicName id="4C644D61FB3DAA61FBA6F895AFE2634B" box="[1131,1234,1862,1879]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="3" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3DAA61FBA6F895AF98634B" bold="true" box="[1131,1192,1862,1879]" italics="true" pageId="3">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
based on near full length 18S rRNA gene sequences. The text in bold blue in the figure represents specimens analyzed in this study. Sequences in light blue are species that have been primarily associated with felid hosts. Green lineages are predominately associated with canid hosts but have been reported in felids. Several sequences derived from domestic cats (i.e., MW578972, PP151898, and PP151899) and wild felids (i.e., HQ187782 and HQ187782) were not included in the analysis because the sequences were short.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BDB36E2FB3AAA66FFA9FEA0AA57659A" blockId="4.[100,771,370,669]" box="[100,359,370,390]" pageId="4">
|
||
<heading id="D093818EFB3AAA66FFA9FEA0AA57659A" box="[100,359,370,390]" fontSize="36" level="2" pageId="4" reason="8">
|
||
<emphasis id="B910EAF0FB3AAA66FFA9FEA0AA57659A" bold="true" box="[100,359,370,390]" italics="true" pageId="4">3.4.2. Partial β- tubulin gene</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FF49FE5CA97D6681" blockId="4.[100,771,370,669]" pageId="4">
|
||
Partial β- tubulin gene sequences (1,101bp) of
|
||
<taxonomicName id="4C644D61FB3AAA66FD8EFE5CA99E65BD" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[579,686,398,418]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FD8EFE5CA99E65BD" bold="true" box="[579,686,398,418]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FD7AFE5CA83365BE" box="[695,771,399,418]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
were obtained from three
|
||
<collectingRegion id="49A0F800FB3AAA66FE97FE78AAAD65A2" box="[346,413,427,446]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas (FP44, FP93, and FP222). The β- tubulin gene sequences from
|
||
<taxonomicName id="4C644D61FB3AAA66FE5BFE14A93365C5" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[406,515,454,474]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FE5BFE14A93365C5" bold="true" box="[406,515,454,474]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FDC1FE14A96965C6" box="[524,601,455,474]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from the
|
||
<collectingRegion id="49A0F800FB3AAA66FD72FE14A83265C6" box="[703,770,455,474]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas were 99.5–99.7% similar to each other. These sequences were 87.3% similar to
|
||
<taxonomicName id="4C644D61FB3AAA66FEE7FE2CAAAE660D" box="[298,414,510,530]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="odocoilei">
|
||
<emphasis id="B910EAF0FB3AAA66FEE7FE2CAAAE660D" bold="true" box="[298,414,510,530]" italics="true" pageId="4">B. odocoilei</emphasis>
|
||
</taxonomicName>
|
||
(of 938-954bp overlapping with AY144706) and 87.2% similar to a
|
||
<taxonomicName id="4C644D61FB3AAA66FE7FFDC9A9286632" box="[434,536,538,558]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FE7FFDC9AAC66631" bold="true" box="[434,502,538,557]" italics="true" pageId="4">Babesia</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
from a Sika deer (
|
||
<taxonomicName id="4C644D61FB3AAA66FD0BFDC9AB906655" authorityName="Temminck" authorityYear="1838" class="Mammalia" family="Cervidae" genus="Cervus" kingdom="Animalia" order="Artiodactyla" pageId="4" phylum="Chordata" rank="species" species="nippon">
|
||
<emphasis id="B910EAF0FB3AAA66FD0BFDC9AB906655" bold="true" italics="true" pageId="4">Cervus nippon</emphasis>
|
||
</taxonomicName>
|
||
) (of 940-956bp overlapping with KC465971) (Supplementary File 2 shows distance matrix with other
|
||
<taxonomicName id="4C644D61FB3AAA66FE11FD81A9606679" box="[476,592,594,613]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FE11FD81A9106679" bold="true" box="[476,544,594,613]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
). Phylogenetically, the three
|
||
<taxonomicName id="4C644D61FB3AAA66FF09FDBDAA00669D" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[196,304,622,641]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola">
|
||
<emphasis id="B910EAF0FB3AAA66FF09FDBDAA00669D" bold="true" box="[196,304,622,641]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
sequences grouped together in a sister clade to
|
||
<taxonomicName id="4C644D61FB3AAA66FFA9FD5AABE66681" box="[100,214,649,669]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FFA9FD5AAB986680" bold="true" box="[100,168,649,668]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
that primarily infect ungulates (
|
||
<figureCitation id="135F2A67FB3AAA66FDC7FD59A90F6681" box="[522,575,650,669]" captionStart="Fig" captionStartId="4.[100,130,1888,1905]" captionTargetBox="[112,758,1215,1855]" captionTargetId="figure-719@4.[106,766,1200,1860]" captionTargetPageId="4" captionText="Fig. 3. Genetic relationships of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) compared with other Babesia spp. based on partial β-tubulin gene sequences. The text in bold in the figure represents specimens analyzed in this study." figureDoi="http://doi.org/10.5281/zenodo.13289457" httpUri="https://zenodo.org/record/13289457/files/figure.png" pageId="4">Fig. 3</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FFA9FD12AA0C66C9" blockId="4.[100,771,705,948]" box="[100,316,705,725]" pageId="4">
|
||
<heading id="D093818EFB3AAA66FFA9FD12AA0C66C9" box="[100,316,705,725]" fontSize="36" level="2" pageId="4" reason="8">
|
||
<emphasis id="B910EAF0FB3AAA66FFA9FD12AA0C66C9" bold="true" box="[100,316,705,725]" italics="true" pageId="4">3.4.3. Partial cytb gene</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FF49FD0EA9DF67A8" blockId="4.[100,771,705,948]" pageId="4">
|
||
Partial cytb gene sequences (1,213bp) of
|
||
<taxonomicName id="4C644D61FB3AAA66FDDDFD0DA94B66EC" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[528,635,733,753]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FDDDFD0DA94B66EC" bold="true" box="[528,635,733,753]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FD4FFD0DA9FE66ED" box="[642,718,734,753]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
were obtained from two
|
||
<collectingRegion id="49A0F800FB3AAA66FEE8FD2AAA586710" box="[293,360,761,780]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas (FP93 and FP222) and they were 99.8% similar to each other. The cytb gene sequences from
|
||
<taxonomicName id="4C644D61FB3AAA66FD55FCC5A8326734" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[664,770,789,809]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FD55FCC5A8326734" bold="true" box="[664,770,789,809]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FFA9FCE1AB816759" box="[100,177,818,837]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from the
|
||
<collectingRegion id="49A0F800FB3AAA66FED9FCE2AA676758" box="[276,343,817,836]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas were 84.6–84.8% similar to
|
||
<taxonomicName id="4C644D61FB3AAA66FD74FCE2A8326758" baseAuthorityName="Piana & Galli-Valerio" baseAuthorityYear="1895" box="[697,770,817,836]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="canis">
|
||
<emphasis id="B910EAF0FB3AAA66FD74FCE2A8326758" bold="true" box="[697,770,817,836]" italics="true" pageId="4">B. canis</emphasis>
|
||
</taxonomicName>
|
||
(KC207822) and 84.4% similar to a
|
||
<taxonomicName id="4C644D61FB3AAA66FDD1FC9EA948677C" box="[540,632,845,864]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="vogeli">
|
||
<emphasis id="B910EAF0FB3AAA66FDD1FC9EA948677C" bold="true" box="[540,632,845,864]" italics="true" pageId="4">B. vogeli</emphasis>
|
||
</taxonomicName>
|
||
(KC207825) (Supplementary File 3 shows distance matrix with other
|
||
<taxonomicName id="4C644D61FB3AAA66FD4FFCBAA9C86760" box="[642,760,873,892]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FD4FFCBAA9F66760" bold="true" box="[642,710,873,892]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
). Phylogenetically, the two
|
||
<taxonomicName id="4C644D61FB3AAA66FEABFC56AAE4678B" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[358,468,900,920]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola">
|
||
<emphasis id="B910EAF0FB3AAA66FEABFC56AAE4678B" bold="true" box="[358,468,900,920]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
sequences grouped as a sister group to a group of
|
||
<taxonomicName id="4C644D61FB3AAA66FEEFFC73AAA467A8" box="[290,404,928,948]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FEEFFC73AA5667AF" bold="true" box="[290,358,928,947]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
of ungulates and carnivores (
|
||
<figureCitation id="135F2A67FB3AAA66FD61FC72A9D267A8" box="[684,738,929,948]" captionStart="Fig" captionStartId="4.[818,848,824,841]" captionTargetBox="[824,1481,148,797]" captionTargetId="figure-765@4.[823,1483,148,797]" captionTargetPageId="4" captionText="Fig. 4. Genetic relationships of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) compared with other Babesia spp. based on partial cytb gene sequences. The text in bold in the figure represents specimens analyzed in this study." figureDoi="http://doi.org/10.5281/zenodo.13289455" httpUri="https://zenodo.org/record/13289455/files/figure.png" pageId="4">Fig. 4</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF1B666AFB3AAA66FCFFFCEBA8BC678A" ID-DOI="http://doi.org/10.5281/zenodo.13289455" ID-Zenodo-Dep="13289455" httpUri="https://zenodo.org/record/13289455/files/figure.png" pageId="4" startId="4.[818,848,824,841]" targetBox="[824,1481,148,797]" targetPageId="4" targetType="figure">
|
||
<paragraph id="8BDB36E2FB3AAA66FCFFFCEBA8BC678A" blockId="4.[818,1487,824,918]" pageId="4">
|
||
<emphasis id="B910EAF0FB3AAA66FCFFFCEBA85C6755" bold="true" box="[818,876,824,841]" pageId="4">Fig. 4.</emphasis>
|
||
Genetic relationships of
|
||
<taxonomicName id="4C644D61FB3AAA66FB8AFCEBAFE46755" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1095,1236,824,841]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FB8AFCEBAFE46755" bold="true" box="[1095,1236,824,841]" italics="true" pageId="4">Babesia coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FB16FCEBAE106754" box="[1243,1312,824,840]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from Florida pumas (
|
||
<emphasis id="B910EAF0FB3AAA66FCF4FC81A8D1677F" bold="true" box="[825,993,849,867]" italics="true" pageId="4">
|
||
Puma
|
||
<taxonomicName id="4C644D61FB3AAA66FCA3FC82A882677E" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[878,946,849,866]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="4" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
|
||
coryi
|
||
</emphasis>
|
||
) compared with other
|
||
<taxonomicName id="4C644D61FB3AAA66FB66FC82AE23677E" box="[1195,1299,849,866]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FB66FC82AFD8677E" bold="true" box="[1195,1256,849,866]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
based on partial cytb gene sequences. The text in bold in the figure represents specimens analyzed in this study.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BDB36E2FB3AAA66FFA9FC0AAA0B67F0" blockId="4.[100,770,984,1143]" box="[100,315,984,1004]" pageId="4">
|
||
<heading id="D093818EFB3AAA66FFA9FC0AAA0B67F0" box="[100,315,984,1004]" fontSize="36" level="2" pageId="4" reason="8">
|
||
<emphasis id="B910EAF0FB3AAA66FFA9FC0AAA0B67F0" bold="true" box="[100,315,984,1004]" italics="true" pageId="4">3.4.4. Partial COI gene</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FF49FC27AF44605D" blockId="4.[100,770,984,1143]" lastBlockId="4.[818,1488,958,1090]" pageId="4">
|
||
Partial COI gene sequences (1,035bp) of
|
||
<taxonomicName id="4C644D61FB3AAA66FDC3FC26A94A601B" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[526,634,1012,1032]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FDC3FC26A94A601B" bold="true" box="[526,634,1012,1032]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FD4CFC26A9FD6014" box="[641,717,1013,1032]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
were obtained from two Florida pumas (FP93 and FP222) and they were 99.7% similar to each other. The COI gene sequences from
|
||
<taxonomicName id="4C644D61FB3AAA66FD5AFBFFA8326023" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[663,770,1068,1087]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FD5AFBFFA8326023" bold="true" box="[663,770,1068,1087]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FFA9FB9AAB806040" box="[100,176,1097,1116]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from the Florida pumas were 89.2% similar to a
|
||
<emphasis id="B910EAF0FB3AAA66FD45FB9BA9AB6047" bold="true" box="[648,667,1096,1115]" italics="true" pageId="4">B.</emphasis>
|
||
sp. from a raccoon
|
||
<emphasis id="B910EAF0FB3AAA66FF7BFBB7AA72606B" bold="true" box="[182,322,1124,1143]" italics="true" pageId="4">
|
||
(
|
||
<taxonomicName id="4C644D61FB3AAA66FF70FBB7AA0B606B" baseAuthorityName="Linnaeus" baseAuthorityYear="1758" box="[189,315,1124,1143]" class="Mammalia" family="Procyonidae" genus="Procyon" kingdom="Animalia" order="Carnivora" pageId="4" phylum="Chordata" rank="species" species="lotor">Procyon lotor</taxonomicName>
|
||
)
|
||
</emphasis>
|
||
(KR017882), 88.8% similar to a
|
||
<emphasis id="B910EAF0FB3AAA66FD48FBB7A9A9606B" bold="true" box="[645,665,1124,1143]" italics="true" pageId="4">B.</emphasis>
|
||
sp. from a captive maned wolf (
|
||
<taxonomicName id="4C644D61FB3AAA66FBCCFC6DAFE567CD" baseAuthorityName="Illiger" baseAuthorityYear="1815" box="[1025,1237,958,977]" class="Mammalia" family="Canidae" genus="Chrysocyon" kingdom="Animalia" order="Carnivora" pageId="4" phylum="Chordata" rank="species" species="brachyurus">
|
||
<emphasis id="B910EAF0FB3AAA66FBCCFC6DAFE567CD" bold="true" box="[1025,1237,958,977]" italics="true" pageId="4">Chrysocyon brachyurus</emphasis>
|
||
</taxonomicName>
|
||
) (KR017881), and 86.2% similar to
|
||
<taxonomicName id="4C644D61FB3AAA66FC6FFC08AF3067F1" box="[930,1024,986,1006]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="gibsoni">
|
||
<emphasis id="B910EAF0FB3AAA66FC6FFC08AF3067F1" bold="true" box="[930,1024,986,1006]" italics="true" pageId="4">B. gibsoni</emphasis>
|
||
</taxonomicName>
|
||
from a domestic dog in
|
||
<collectingCountry id="F3737672FB3AAA66FADBFC08AE7E67F2" box="[1302,1358,987,1006]" name="Japan" pageId="4">Japan</collectingCountry>
|
||
(AB685183) (Supplementary File 4 shows distance matrix with other
|
||
<taxonomicName id="4C644D61FB3AAA66FA9DFC25AEF56016" box="[1360,1477,1014,1034]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FA9DFC25AEA46015" bold="true" box="[1360,1428,1014,1033]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
). Phylogenetically, the two
|
||
<taxonomicName id="4C644D61FB3AAA66FBE9FBC1AFBD6039" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1060,1165,1042,1061]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola">
|
||
<emphasis id="B910EAF0FB3AAA66FBE9FBC1AFBD6039" bold="true" box="[1060,1165,1042,1061]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
sequences grouped with a clade of
|
||
<taxonomicName id="4C644D61FB3AAA66FCFFFBFDA8A7605E" box="[818,919,1070,1090]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FCFFFBFDA846605D" bold="true" box="[818,886,1070,1089]" italics="true" pageId="4">Babesia</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
from raccoons (
|
||
<figureCitation id="135F2A67FB3AAA66FBFCFBFDAF56605D" box="[1073,1126,1070,1089]" captionStart="Fig" captionStartId="5.[100,130,1082,1099]" captionTargetBox="[340,1246,149,1053]" captionTargetId="figure-569@5.[338,1248,148,1055]" captionTargetPageId="5" captionText="Fig. 5. Genetic relationships of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) compared with other Babesia spp. based on partial COI gene sequences. The text in bold in the figure represents specimens analyzed in this study." figureDoi="http://doi.org/10.5281/zenodo.13289459" httpUri="https://zenodo.org/record/13289459/files/figure.png" pageId="4">Fig. 5</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FCFFFBB5AF2B6065" blockId="4.[818,1488,1126,1396]" box="[818,1051,1126,1145]" pageId="4">
|
||
<emphasis id="B910EAF0FB3AAA66FCFFFBB5AF2B6065" bold="true" box="[818,1051,1126,1145]" italics="true" pageId="4">3.4.5. Partial COX3 gene</emphasis>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FC9CFB51AEA86168" blockId="4.[818,1488,1126,1396]" pageId="4">
|
||
Partial COX3 gene sequences (717bp) of
|
||
<taxonomicName id="4C644D61FB3AAA66FB11FB51AE776088" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1244,1351,1153,1173]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FB11FB51AE776088" bold="true" box="[1244,1351,1153,1173]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FA82FB51AEAB6089" box="[1359,1435,1154,1173]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
were obtained from four
|
||
<collectingRegion id="49A0F800FB3AAA66FC3DFB4DAF0360AD" box="[1008,1075,1182,1201]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas (FP93, FP222, FP233, and FP235). Each sequence was unique and were 99.4–99.9% similar to each other. The COX3 gene sequences from
|
||
<taxonomicName id="4C644D61FB3AAA66FBA7FB05AFE660F4" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1130,1238,1237,1257]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FBA7FB05AFE660F4" bold="true" box="[1130,1238,1237,1257]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FB13FB05AE1A60F5" box="[1246,1322,1238,1257]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from the
|
||
<collectingRegion id="49A0F800FB3AAA66FA41FB06AEFF60F4" box="[1420,1487,1237,1256]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas were most similar to
|
||
<taxonomicName id="4C644D61FB3AAA66FB8CFB21AFB86118" baseAuthorityName="Piana & Galli-Valerio" baseAuthorityYear="1895" box="[1089,1160,1265,1285]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="canis">
|
||
<emphasis id="B910EAF0FB3AAA66FB8CFB21AFB86118" bold="true" box="[1089,1160,1265,1285]" italics="true" pageId="4">B. canis</emphasis>
|
||
</taxonomicName>
|
||
(83.9–84.4% similar) followed by several strains of
|
||
<taxonomicName id="4C644D61FB3AAA66FC1BFADDAF1C613C" box="[982,1068,1293,1313]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="gibsoni">
|
||
<emphasis id="B910EAF0FB3AAA66FC1BFADDAF1C613C" bold="true" box="[982,1068,1293,1313]" italics="true" pageId="4">B. gibsoni</emphasis>
|
||
</taxonomicName>
|
||
(~81–82.8% similar) (Supplementary File 5 shows distance matrix with other
|
||
<taxonomicName id="4C644D61FB3AAA66FBB0FAFAAFC46120" box="[1149,1268,1321,1340]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FBB0FAFAAFF16120" bold="true" box="[1149,1217,1321,1340]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
). Phylogenetically, the two
|
||
<taxonomicName id="4C644D61FB3AAA66FC96FA96A8F56144" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[859,965,1349,1368]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola">
|
||
<emphasis id="B910EAF0FB3AAA66FC96FA96A8F56144" bold="true" box="[859,965,1349,1368]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
sequences grouped with a clade of
|
||
<taxonomicName id="4C644D61FB3AAA66FAD8FA96AE5B6144" box="[1301,1387,1349,1368]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="gibsoni">
|
||
<emphasis id="B910EAF0FB3AAA66FAD8FA96AE5B6144" bold="true" box="[1301,1387,1349,1368]" italics="true" pageId="4">B. gibsoni</emphasis>
|
||
</taxonomicName>
|
||
sequences and this group was a sister clade to
|
||
<taxonomicName id="4C644D61FB3AAA66FB48FAB2AFFB6168" baseAuthorityName="Piana & Galli-Valerio" baseAuthorityYear="1895" box="[1157,1227,1377,1396]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="canis">
|
||
<emphasis id="B910EAF0FB3AAA66FB48FAB2AFFB6168" bold="true" box="[1157,1227,1377,1396]" italics="true" pageId="4">B. canis</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C644D61FB3AAA66FB36FAB2AE766168" box="[1275,1350,1377,1396]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="vogeli">
|
||
<emphasis id="B910EAF0FB3AAA66FB36FAB2AE766168" bold="true" box="[1275,1350,1377,1396]" italics="true" pageId="4">B. vogeli</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="135F2A67FB3AAA66FA98FAB2AEBA6168" box="[1365,1418,1377,1396]" captionStart="Fig" captionStartId="6.[100,130,862,879]" captionTargetBox="[110,767,148,834]" captionTargetId="figure-1202@6.[106,767,148,834]" captionTargetPageId="6" captionText="Fig. 6. Genetic relationships of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) compared with other Babesia spp. based on partial COX3 gene sequences. The text in bold in the figure represents specimens analyzed in this study." figureDoi="http://doi.org/10.5281/zenodo.13289461" httpUri="https://zenodo.org/record/13289461/files/figure.png" pageId="4">Fig. 6</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FCFFFA4AAF7261B0" blockId="4.[818,1488,1432,1564]" box="[818,1090,1432,1452]" pageId="4">
|
||
<emphasis id="B910EAF0FB3AAA66FCFFFA4AAF7261B0" bold="true" box="[818,1090,1432,1452]" italics="true" pageId="4">3.4.6. Partial 28S rRNA gene</emphasis>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FC9CFA66AEE06207" blockId="4.[818,1488,1432,1564]" pageId="4">
|
||
A short region of the 28S rRNA gene (245bp) was obtained for
|
||
<taxonomicName id="4C644D61FB3AAA66FCFFFA02A8AC61FF" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[818,924,1488,1508]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FCFFFA02A8AC61FF" bold="true" box="[818,924,1488,1508]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FC6FFA02A8DC61F8" box="[930,1004,1489,1508]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from one
|
||
<collectingRegion id="49A0F800FB3AAA66FB80FA02AFA061F8" box="[1101,1168,1489,1508]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas (FP93). The sequence was most similar (95.5%) to a
|
||
<taxonomicName id="4C644D61FB3AAA66FBFBFA3FAFAE621C" box="[1078,1182,1516,1536]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FBFBFA3FAF4A61E3" bold="true" box="[1078,1146,1516,1535]" italics="true" pageId="4">Babesia</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
from a red panda (OK524314) followed by
|
||
<taxonomicName id="4C644D61FB3AAA66FC6BF9DAAF3E6207" baseAuthorityName="Smith & Kilborne" baseAuthorityYear="1893" box="[934,1038,1544,1564]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="bigemina">
|
||
<emphasis id="B910EAF0FB3AAA66FC6BF9DAAF3E6207" bold="true" box="[934,1038,1544,1564]" italics="true" pageId="4">B. bigemina</emphasis>
|
||
</taxonomicName>
|
||
(90.3%, LK391709) and
|
||
<taxonomicName id="4C644D61FB3AAA66FB31F9DAAE626207" box="[1276,1362,1544,1564]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="gibsoni">
|
||
<emphasis id="B910EAF0FB3AAA66FB31F9DAAE626207" bold="true" box="[1276,1362,1544,1564]" italics="true" pageId="4">B. gibsoni</emphasis>
|
||
</taxonomicName>
|
||
(CP141527).
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FCFFF993AF7A624F" blockId="4.[818,1488,1600,1954]" box="[818,1098,1600,1619]" pageId="4">
|
||
<emphasis id="B910EAF0FB3AAA66FCFFF993AF7A624F" bold="true" box="[818,1098,1600,1619]" italics="true" pageId="4">3.4.7. ITS-1 and ITS-2 regions</emphasis>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3AAA66FC9CF98FAF0F6352" blockId="4.[818,1488,1600,1954]" pageId="4">
|
||
The ITS-1 rRNA sequences of
|
||
<taxonomicName id="4C644D61FB3AAA66FBBFF98FAFED6273" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1138,1245,1628,1647]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FBBFF98FAFED6273" bold="true" box="[1138,1245,1628,1647]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FB28F98FAE016273" box="[1253,1329,1628,1647]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from 23
|
||
<collectingRegion id="49A0F800FB3AAA66FA41F98FAEFF6273" box="[1420,1487,1628,1647]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
pumas were all unique and percent identity ranged from 85.7 to 99.5%. The sequences varied in size (641-657bp) due to numerous 1-15bp insertions/deletions. No apparent association was noted between ITS-1 sequences and county of origin or year of sampling (range from 1992 to 2005). The
|
||
<taxonomicName id="4C644D61FB3AAA66FC7EF93BAF2C62E6" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[947,1052,1767,1787]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FC7EF93BAF2C62E6" bold="true" box="[947,1052,1767,1787]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FBEDF93BAF5962E7" box="[1056,1129,1768,1787]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
ITS-1 sequences were 87.2–89.4% and 86.8–87.9% identical to
|
||
<taxonomicName id="4C644D61FB3AAA66FBE2F8D7AFAE630A" box="[1071,1182,1795,1815]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="odocoilei">
|
||
<emphasis id="B910EAF0FB3AAA66FBE2F8D7AFAE630A" bold="true" box="[1071,1182,1795,1815]" italics="true" pageId="4">B. odocoilei</emphasis>
|
||
</taxonomicName>
|
||
(AY339751) and
|
||
<taxonomicName id="4C644D61FB3AAA66FA90F8D7AEE0630A" authorityName="M'Fadyean. & Stockman" authorityYear="1911" box="[1373,1488,1795,1815]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="divergens">
|
||
<emphasis id="B910EAF0FB3AAA66FA90F8D7AEE0630A" bold="true" box="[1373,1488,1795,1815]" italics="true" pageId="4">B. divergens</emphasis>
|
||
</taxonomicName>
|
||
(EF458168), respectively. A representative sequence was submitted to GenBank (KJ592628, FP40).
|
||
</paragraph>
|
||
<caption id="DF1B666AFB3AAA66FFA9F8B3AA1763A1" ID-DOI="http://doi.org/10.5281/zenodo.13289457" ID-Zenodo-Dep="13289457" httpUri="https://zenodo.org/record/13289457/files/figure.png" pageId="4" startId="4.[100,130,1888,1905]" targetBox="[112,758,1215,1855]" targetPageId="4" targetType="figure">
|
||
<paragraph id="8BDB36E2FB3AAA66FFA9F8B3AA1763A1" blockId="4.[100,770,1887,1981]" pageId="4">
|
||
<emphasis id="B910EAF0FB3AAA66FFA9F8B3ABAF636D" bold="true" box="[100,159,1888,1905]" pageId="4">Fig. 3.</emphasis>
|
||
Genetic relationships of
|
||
<taxonomicName id="4C644D61FB3AAA66FEB7F88CA937636C" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[378,519,1887,1904]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FEB7F88CA937636C" bold="true" box="[378,519,1887,1904]" italics="true" pageId="4">Babesia coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FDC3F8B3A963636C" box="[526,595,1888,1904]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from Florida pumas (
|
||
<emphasis id="B910EAF0FB3AAA66FFA6F8A9AA2C6396" bold="true" box="[107,284,1913,1930]" italics="true" pageId="4">
|
||
Puma
|
||
<taxonomicName id="4C644D61FB3AAA66FF68F8AAABD96396" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[165,233,1913,1930]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="4" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
|
||
coryi
|
||
</emphasis>
|
||
) compared with other
|
||
<taxonomicName id="4C644D61FB3AAA66FE3AF8AAA9546396" box="[503,612,1913,1930]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3AAA66FE3AF8AAA9046396" bold="true" box="[503,564,1913,1930]" italics="true" pageId="4">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
based on partial β- tubulin gene sequences. The text in bold in the figure represents specimens analyzed in this study.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BDB36E2FB3AAA67FC9CF884A9A060E9" blockId="4.[818,1488,1600,1954]" lastBlockId="5.[100,770,1166,1269]" lastPageId="5" pageId="4">
|
||
The ITS-2 rRNA sequence of
|
||
<taxonomicName id="4C644D61FB3AAA66FBACF884AFFA6376" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[1121,1226,1879,1898]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="4" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3AAA66FBACF884AFFA6376" bold="true" box="[1121,1226,1879,1898]" italics="true" pageId="4">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3AAA66FB1CF88BAE2B6377" box="[1233,1307,1880,1899]" pageId="4" rank="species">sp. nov.</taxonomicNameLabel>
|
||
was obtained from one
|
||
<collectingRegion id="49A0F800FB3AAA66FC95F8A0A8AB639A" box="[856,923,1907,1926]" country="United States of America" name="Florida" pageId="4">Florida</collectingRegion>
|
||
puma (KJ592629; FP40). It was 244 bp long and contained a single polymorphic base. The ITS-2 sequence was 96% similar to
|
||
<taxonomicName id="4C644D61FB3BAA67FFA9FB5DABFC60BD" baseAuthorityName="Smith & Kilborne" baseAuthorityYear="1893" box="[100,204,1166,1185]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="species" species="bigemina">
|
||
<emphasis id="B910EAF0FB3BAA67FFA9FB5DABFC60BD" bold="true" box="[100,204,1166,1185]" italics="true" pageId="5">B. bigemina</emphasis>
|
||
</taxonomicName>
|
||
(e.g., MH050926), 92% similar to a
|
||
<emphasis id="B910EAF0FB3BAA67FDEAFB5DA90A60BD" bold="true" box="[551,570,1166,1185]" italics="true" pageId="5">B.</emphasis>
|
||
sp. from a red panda (OK524314) and
|
||
<taxonomicName id="4C644D61FB3BAA67FEC2FB7AAA4860A1" box="[271,376,1193,1213]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3BAA67FEC2FB7AAA6360A0" bold="true" box="[271,339,1193,1212]" italics="true" pageId="5">Babesia</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
RD61 from a captive reindeer (
|
||
<taxonomicName id="4C644D61FB3BAA67FD7AFB79AB8260C4" baseAuthorityName="Linnaeus" baseAuthorityYear="1758" class="Mammalia" family="Cervidae" genus="Rangifer" kingdom="Animalia" order="Artiodactyla" pageId="5" phylum="Chordata" rank="species" species="tarandus">
|
||
<emphasis id="B910EAF0FB3BAA67FD7AFB79AB8260C4" bold="true" italics="true" pageId="5">Rangifer tarandus</emphasis>
|
||
</taxonomicName>
|
||
) from
|
||
<collectingRegion id="49A0F800FB3BAA67FF3BFB15AA6360C5" box="[246,339,1222,1241]" country="United States of America" name="California" pageId="5">California</collectingRegion>
|
||
(AY339744), and 91% similar to
|
||
<taxonomicName id="4C644D61FB3BAA67FD54FB15A83260C4" box="[665,770,1221,1241]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="species" species="odocoilei">
|
||
<emphasis id="B910EAF0FB3BAA67FD54FB15A83260C4" bold="true" box="[665,770,1221,1241]" italics="true" pageId="5">B. odocoilei</emphasis>
|
||
</taxonomicName>
|
||
from a
|
||
<collectingRegion id="49A0F800FB3BAA67FF64FB31AA3B60E9" box="[169,267,1250,1269]" country="United States of America" name="Minnesota" pageId="5">Minnesota</collectingRegion>
|
||
caribou
|
||
<emphasis id="B910EAF0FB3BAA67FE93FB32A93F60E8" bold="true" box="[350,527,1249,1268]" italics="true" pageId="5">
|
||
(
|
||
<taxonomicName id="4C644D61FB3BAA67FEA9FB32A93860E8" baseAuthorityName="Linnaeus" baseAuthorityYear="1758" box="[356,520,1249,1268]" class="Mammalia" family="Cervidae" genus="Rangifer" kingdom="Animalia" order="Artiodactyla" pageId="5" phylum="Chordata" rank="species" species="tarandus">Rangifer tarandus</taxonomicName>
|
||
)
|
||
</emphasis>
|
||
(AY339758).
|
||
</paragraph>
|
||
<caption id="DF1B666AFB3BAA67FFA9FBE9A8716079" ID-DOI="http://doi.org/10.5281/zenodo.13289459" ID-Zenodo-Dep="13289459" httpUri="https://zenodo.org/record/13289459/files/figure.png" pageId="5" startId="5.[100,130,1082,1099]" targetBox="[340,1246,149,1053]" targetPageId="5" targetType="figure">
|
||
<paragraph id="8BDB36E2FB3BAA67FFA9FBE9A8716079" blockId="5.[100,1487,1082,1125]" pageId="5">
|
||
<emphasis id="B910EAF0FB3BAA67FFA9FBE9ABAF6057" bold="true" box="[100,159,1082,1099]" pageId="5">Fig. 5.</emphasis>
|
||
Genetic relationships of
|
||
<taxonomicName id="4C644D61FB3BAA67FEB7FBE9A9376057" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[378,519,1082,1099]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3BAA67FEB7FBE9A9376057" bold="true" box="[378,519,1082,1099]" italics="true" pageId="5">Babesia coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3BAA67FDC3FBE8A9636057" box="[526,595,1083,1099]" pageId="5" rank="species">sp. nov.</taxonomicNameLabel>
|
||
from Florida pumas (
|
||
<emphasis id="B910EAF0FB3BAA67FCDDFBE8A8876050" bold="true" box="[784,951,1082,1100]" italics="true" pageId="5">
|
||
Puma
|
||
<taxonomicName id="4C644D61FB3BAA67FC88FBE9A8B96057" baseAuthorityName="Keyserling" baseAuthorityYear="1891" box="[837,905,1082,1099]" class="Mammalia" family="Felidae" genus="Panthera" kingdom="Animalia" order="Carnivora" pageId="5" phylum="Chordata" rank="species" species="concolor">concolor</taxonomicName>
|
||
coryi
|
||
</emphasis>
|
||
) compared with other
|
||
<taxonomicName id="4C644D61FB3BAA67FB4CFBE9AFD96057" box="[1153,1257,1082,1099]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="species" species="undetermined">
|
||
<emphasis id="B910EAF0FB3BAA67FB4CFBE9AF8E6057" bold="true" box="[1153,1214,1082,1099]" italics="true" pageId="5">Babesia</emphasis>
|
||
spp.
|
||
</taxonomicName>
|
||
based on partial COI gene sequences. The text in bold in the figure represents specimens analyzed in this study.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BDB36E2FB3BAA67FFA9FAF3AAFD612F" blockId="5.[100,461,1312,1331]" box="[100,461,1312,1331]" pageId="5">
|
||
<heading id="D093818EFB3BAA67FFA9FAF3AAFD612F" box="[100,461,1312,1331]" fontSize="36" level="2" pageId="5" reason="3">
|
||
<emphasis id="B910EAF0FB3BAA67FFA9FAF3AAFD612F" bold="true" box="[100,461,1312,1331]" italics="true" pageId="5">3.5. Assessment of vertical transmission</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3BAA67FF49FA8BABA361BF" blockId="5.[100,770,1368,1443]" pageId="5">
|
||
All 24 kittens were PCR negative for piroplasms. During the year of sampling, all mothers were confirmed positive for
|
||
<taxonomicName id="4C644D61FB3BAA67FDFEFAA7A9AB619B" authorityName="Shock & Jones & Garrett & Hernandez & Burchfield & Haman & Schwantje & Telford & Cunningham & Yabsley" authorityYear="2024" box="[563,667,1396,1415]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="species" species="coryicola" status="sp. nov.">
|
||
<emphasis id="B910EAF0FB3BAA67FDFEFAA7A9AB619B" bold="true" box="[563,667,1396,1415]" italics="true" pageId="5">B. coryicola</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A223578BFB3BAA67FD52FAA6A9D76194" box="[671,743,1397,1416]" pageId="5" rank="species">sp. nov.</taxonomicNameLabel>
|
||
by PCR.
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3BAA67FFA9FA1CAA4761FD" blockId="5.[100,375,1486,1506]" box="[100,375,1486,1506]" pageId="5">
|
||
<heading id="D093818EFB3BAA67FFA9FA1CAA4761FD" box="[100,375,1486,1506]" fontSize="36" level="2" pageId="5" reason="3">
|
||
<emphasis id="B910EAF0FB3BAA67FFA9FA1CAA4761FD" bold="true" box="[100,375,1486,1506]" italics="true" pageId="5">3.6. Hematological evaluation</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BDB36E2FB3BAA67FF49F9D4AAF1624D" blockId="5.[100,770,1542,1617]" pageId="5">
|
||
No hematological differences were detected between
|
||
<taxonomicName id="4C644D61FB3BAA67FD4FF9D5A9F66205" box="[642,710,1542,1561]" family="Babesiidae" genus="Babesia" kingdom="Chromista" order="Piroplasmida" pageId="5" phylum="Miozoa" rank="genus">
|
||
<emphasis id="B910EAF0FB3BAA67FD4FF9D5A9F66205" bold="true" box="[642,710,1542,1561]" italics="true" pageId="5">Babesia</emphasis>
|
||
</taxonomicName>
|
||
-infected
|
||
<collectingRegion id="49A0F800FB3BAA67FF4AF9F1ABFA6229" box="[135,202,1570,1589]" country="United States of America" name="Florida" pageId="5">Florida</collectingRegion>
|
||
pumas included in this study and the seven groups of pumas from the five other studies (
|
||
<tableCitation id="C6E60359FB3BAA67FEA0F9EDAA83624D" box="[365,435,1598,1617]" captionStart="Table 2" captionStartId="7.[100,150,150,166]" captionTargetBox="[116,1471,238,1338]" captionText="Table 2 Hematological values for 25 Babesia coryicola sp. nov.-infected Florida pumas (Puma concolor coryi) compared to hematological values for Puma concolor from previous studies." pageId="5">Table 2</tableCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |