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<document id="147A503DCA38CA84F33C871F196F8C89" ID-DOI="10.11646/phytotaxa.233.1.2" ID-ISSN="1179-3163" ID-Zenodo-Dep="13631906" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1725314418116" checkinUser="felipe" docAuthor="Nguanhom, Jeerapa, Cheewangkoon, Ratchadawan, Groenewald, Johannes Z., Braun, Uwe, To-Anun, Chaiwat &amp; Crous, Pedro W." docDate="2015" docId="03B087884E13F16694B8CDB2E8A23F24" docLanguage="en" docName="phytotaxa.233.1.2.pdf" docOrigin="Phytotaxa 233 (1)" docSource="http://dx.doi.org/10.11646/phytotaxa.233.1.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Cercospora glycinicola Cheew., Crous &amp; U. Braun 2015, sp. nov." docType="treatment" docVersion="2" lastPageNumber="39" masterDocId="FF89FFF04E1FF16B9430C906EA223E25" masterDocTitle="Taxonomy and phylogeny of Cercospora spp. from Northern Thailand" masterLastPageNumber="48" masterPageNumber="27" pageNumber="39" updateTime="1725314850666" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="CD331141CFC85928D7A8AF03496AEC87">Taxonomy and phylogeny of Cercospora spp. from Northern Thailand</mods:title>
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<mods:namePart id="1446DAC704DB544D0F6A3FBD5F60E909">Nguanhom, Jeerapa</mods:namePart>
<mods:affiliation id="ECD8F71356AC4B0E5E1EFDA90666F33F">Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, 50200, Thailand</mods:affiliation>
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<mods:namePart id="3908071F0FF459C51E83EFF9134C5856">Cheewangkoon, Ratchadawan</mods:namePart>
<mods:affiliation id="DF92F478D8ED15D3AB9598FC20CF057D">Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, 50200, Thailand</mods:affiliation>
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<mods:namePart id="D642498026860E2E0F0686880439DFFC">Groenewald, Johannes Z.</mods:namePart>
<mods:affiliation id="00DB8D11D5A4785BC62560792DE518E5">CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands</mods:affiliation>
</mods:name>
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<mods:namePart id="82B392743B9E287BEBA1D5E40217BCE9">Braun, Uwe</mods:namePart>
<mods:affiliation id="57AA491AA843557B8017DB329E5F9397">Martin-Luther-Universität, Institut für Biologie, Bereich Geobotanik und Botanischer Garten, Herbarium, Neuwerk 21, 06099 Halle (Saale), Germany</mods:affiliation>
</mods:name>
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<mods:namePart id="E755D3FFB63CBDC94623508A8F386272">To-Anun, Chaiwat</mods:namePart>
<mods:affiliation id="BA67A6611D7874C1AD9DFB7F02A35CB0">Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, 50200, Thailand</mods:affiliation>
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<mods:namePart id="F68E2AE6E6B25268B85F0FD780273A9C">Crous, Pedro W.</mods:namePart>
<mods:affiliation id="01F654768D6174EF771BD3C568436EAF">CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands &amp; Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa</mods:affiliation>
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<mods:title id="3C342D63D46D26F45FDE20BCB0413120">Phytotaxa</mods:title>
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<mods:date id="A4CF9F2D27FBB713465B1EF0DE04C5D2">2015</mods:date>
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<mods:identifier id="B0BE0A08E06C32221785069F320ED2FF" type="Zenodo-Dep">13631906</mods:identifier>
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<treatment id="03B087884E13F16694B8CDB2E8A23F24" ID-DOI="http://doi.org/10.5281/zenodo.13631910" ID-Zenodo-Dep="13631910" LSID="urn:lsid:plazi:treatment:03B087884E13F16694B8CDB2E8A23F24" httpUri="http://treatment.plazi.org/id/03B087884E13F16694B8CDB2E8A23F24" lastPageId="13" lastPageNumber="39" pageId="12" pageNumber="39">
<subSubSection id="C30365154E13F16794B8CDB2EEB73AEA" box="[136,1173,1203,1231]" pageId="12" pageNumber="39" type="nomenclature">
<paragraph id="8BA6369E4E13F16794B8CDB2EEB73AEA" blockId="12.[136,1173,1203,1231]" box="[136,1173,1203,1231]" pageId="12" pageNumber="39">
<heading id="D0EE81F24E13F16794B8CDB2EEB73AEA" box="[136,1173,1203,1231]" fontSize="11" level="2" pageId="12" pageNumber="39" reason="3">
<taxonomicName id="4C194D1D4E13F16794B8CDB2E8EB3AEA" authority="Cheew., Crous &amp; U. Braun" authorityName="Cheew., Crous &amp; U. Braun" authorityYear="2015" box="[136,713,1203,1231]" class="Leotiomycetes" family="Dermateaceae" genus="Cercospora" kingdom="Fungi" order="Helotiales" pageId="12" pageNumber="39" phylum="Ascomycota" rank="species" species="glycinicola" status="sp. nov.">
<emphasis id="B96DEA8C4E13F16794B8CDB2EBB33AEB" bold="true" box="[136,401,1203,1230]" italics="true" pageId="12" pageNumber="39">Cercospora glycinicola</emphasis>
Cheew., Crous &amp; U. Braun
</taxonomicName>
,
<emphasis id="B96DEA8C4E13F16796E5CDB0E90B3AEA" box="[725,809,1206,1231]" italics="true" pageId="12" pageNumber="39">
<taxonomicNameLabel id="A25E57F74E13F16796E5CDB0E90B3AEA" box="[725,809,1206,1231]" pageId="12" pageNumber="39" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(
<figureCitation id="13222A1B4E13F1679708CDB0E95F3AEA" box="[824,893,1206,1231]" captionStart="FIGURE 2" captionStartId="13.[136,229,1231,1252]" captionTargetBox="[151,1436,281,1206]" captionTargetId="figure-62@13.[151,1436,281,1206]" captionTargetPageId="13" captionText="FIGURE 2. Cercospora glycinicola (CBS H-22289).A. Leaf spot; B. Close-up of leaf spot; C, D. Conidiophores and conidiogenous cells; EI. Conidia; J. Colony on MEA.—Scale bars: CD = 40 μm; EI = 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13631914" httpUri="https://zenodo.org/record/13631914/files/figure.png" pageId="12" pageNumber="39">Fig. 2</figureCitation>
). MycoBank MB812918
</heading>
</paragraph>
</subSubSection>
<subSubSection id="C30365154E13F16794B8CC06EB1C3916" pageId="12" pageNumber="39" type="materials_examined">
<paragraph id="8BA6369E4E13F16794B8CC06E83A3B1C" blockId="12.[136,1451,1280,1337]" pageId="12" pageNumber="39">
<emphasis id="B96DEA8C4E13F16794B8CC06EAE03B30" bold="true" box="[136,194,1280,1301]" pageId="12" pageNumber="39">Type:</emphasis>
<collectingCountry id="F30E760E4E13F16794EBCC06EB7B3B30" box="[219,345,1280,1301]" name="Thailand" pageId="12" pageNumber="39">THAILAND</collectingCountry>
.
<collectingRegion id="49DDF87C4E13F1679552CC06EBF53B30" box="[354,471,1280,1301]" country="Thailand" name="Chiang Mai" pageId="12" pageNumber="39">Chiang Mai</collectingRegion>
: on
<taxonomicName id="4C194D1D4E13F16795CDCC06E8573B30" box="[509,629,1280,1301]" class="Magnoliopsida" family="Fabaceae" genus="Glycine" kingdom="Plantae" order="Fabales" pageId="12" pageNumber="39" phylum="Tracheophyta" rank="species" species="max">
<emphasis id="B96DEA8C4E13F16795CDCC06E8573B30" box="[509,629,1280,1301]" italics="true" pageId="12" pageNumber="39">Glycine max</emphasis>
</taxonomicName>
(
<taxonomicName id="4C194D1D4E13F16796B1CC06E8C23B30" box="[641,736,1280,1301]" class="Magnoliopsida" family="Fabaceae" kingdom="Plantae" order="Fabales" pageId="12" pageNumber="39" phylum="Tracheophyta" rank="family">Fabaceae</taxonomicName>
),
<date id="FFA7105E4E13F16796DFCC06E9533B30" box="[751,881,1280,1301]" pageId="12" pageNumber="39" value="2013-03-29">29 Mar. 2013</date>
,
<emphasis id="B96DEA8C4E13F167974ACC06E9CC3B30" box="[890,1006,1280,1301]" italics="true" pageId="12" pageNumber="39">
<collectionCode id="ED08AE5B4E13F167974ACC06E9AB3B30" box="[890,905,1280,1301]" country="Sweden" lsid="urn:lsid:biocol.org:col:15668" name="Department of Botany, Swedish Museum of Natural History" pageId="12" pageNumber="39" type="Herbarium">S</collectionCode>
. Seekanha
</emphasis>
(
<typeStatus id="54A2883C4E13F16797CACC06EE723B30" box="[1018,1104,1280,1301]" pageId="12" pageNumber="39" type="holotype">holotype</typeStatus>
<collectionCode id="ED08AE5B4E13F1679065CC06EEA03B30" box="[1109,1154,1280,1301]" country="Netherlands" httpUri="http://grbio.org/cool/bxq2-d2kh" name="Centraalbureau voor Schimmelcultures, Fungal and Yeast Collection" pageId="12" pageNumber="39">CBS</collectionCode>
H-22289, culture ex-type
<collectionCode id="ED08AE5B4E13F167914ECC06EF893B30" box="[1406,1451,1280,1301]" country="Netherlands" httpUri="http://grbio.org/cool/3snx-0r7t" name="Culture collection of Pedro Crous" pageId="12" pageNumber="39">CPC</collectionCode>
23911 =
<collectionCode id="ED08AE5B4E13F1679522CC22EB1D3B1C" box="[274,319,1316,1337]" country="Netherlands" httpUri="http://grbio.org/cool/bxq2-d2kh" name="Centraalbureau voor Schimmelcultures, Fungal and Yeast Collection" pageId="12" pageNumber="39">CBS</collectionCode>
140164,
<collectionCode id="ED08AE5B4E13F16795A9CC22EBE43B1C" box="[409,454,1316,1337]" country="Netherlands" httpUri="http://grbio.org/cool/3snx-0r7t" name="Culture collection of Pedro Crous" pageId="12" pageNumber="39">CPC</collectionCode>
23912).
</paragraph>
<paragraph id="8BA6369E4E13F16794B8CC6CEAD73886" blockId="12.[136,1452,1386,2059]" pageId="12" pageNumber="39">
<emphasis id="B96DEA8C4E13F16794B8CC6CEADE3BA6" box="[136,252,1386,1411]" italics="true" pageId="12" pageNumber="39">Leaf spots</emphasis>
amphigenous, subcircular to irregular, pale brown, surrounded by a darker brown margin,
<quantity id="4CE19B7B4E13F1679135CC6CEF463BA6" box="[1285,1380,1386,1411]" metricMagnitude="-3" metricUnit="m" metricValue="2.0" metricValueMax="3.0" metricValueMin="1.0" pageId="12" pageNumber="39" unit="mm" value="2.0" valueMax="3.0" valueMin="1.0">13 mm</quantity>
diam. Mycelium internal.
<emphasis id="B96DEA8C4E13F167955FCC88EBC03B82" box="[367,482,1422,1447]" italics="true" pageId="12" pageNumber="39">Caespituli</emphasis>
amphigenous, punctiform, brown.
<emphasis id="B96DEA8C4E13F16797B0CC88E9C43B82" box="[896,998,1422,1447]" italics="true" pageId="12" pageNumber="39">Stromata</emphasis>
brown, intraepidermal or substomatal, 32.557.5 μm diam.
<emphasis id="B96DEA8C4E13F167954DCCB4E8003BEE" box="[381,546,1458,1483]" italics="true" pageId="12" pageNumber="39">Conidiophores</emphasis>
in moderately dense fascicles (425), straight or sinuous to geniculate due to sympodial proliferation, unbranched, brown, paler toward the apex, 30113 × 46 μm, 14-septate.
<emphasis id="B96DEA8C4E13F1679132CCD0EA993836" italics="true" pageId="12" pageNumber="39">Conidiogenous cells</emphasis>
proliferating sympodially, integrated, terminal or conidiophores reduced to conidiogenous cells, 2860 μm long; conidiogenous loci conspicuous, apical and formed on shoulders caused by geniculation, lateral, multi-local, loci distinctly thickened, darkened, 34 μm diam.
<emphasis id="B96DEA8C4E13F1679692CF44E8DE387E" box="[674,764,1602,1627]" italics="true" pageId="12" pageNumber="39">Conidia</emphasis>
solitary, obclavate-cylindrical, hyaline, obtuse at the apex, truncate to slightly obconically truncate at the base, 13-septate, 2383 × 35 μm; hila thickened and darkened, 2.53 μm diam.
</paragraph>
<paragraph id="8BA6369E4E13F167948DCFA8EB1C3916" blockId="12.[136,1452,1386,2059]" pageId="12" pageNumber="39">
<emphasis id="B96DEA8C4E13F167948DCFA8EBFB38E2" bold="true" box="[189,473,1710,1735]" pageId="12" pageNumber="39">Culture characteristics:</emphasis>
—Colonies spreading, with moderate to dense, felty aerial mycelium, entire to undulate margin, reaching
<quantity id="4CE19B7B4E13F167957FCFD4EB8238CE" box="[335,416,1746,1771]" metricMagnitude="-2" metricUnit="m" metricValue="4.2" pageId="12" pageNumber="39" unit="mm" value="42.0">42 mm</quantity>
diam after 2 wk. On MEA white, with somewhat greyish pink exudates. On
<collectionCode id="ED08AE5B4E13F1679120CFD4EF6438CE" box="[1296,1350,1746,1771]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="12" pageNumber="39" type="Herbarium">PDA</collectionCode>
white to slightly grey, with diffuse red pigment in agar surrounding colony. On OA white to slightly grey, with diffuse red pigment in agar.
</paragraph>
</subSubSection>
<subSubSection id="C30365154E13F166948DCE38E8A23F24" lastPageId="13" lastPageNumber="40" pageId="12" pageNumber="39" type="etymology">
<paragraph id="8BA6369E4E13F167948DCE38E9123972" blockId="12.[136,1452,1386,2059]" box="[189,816,1854,1879]" pageId="12" pageNumber="39">
<emphasis id="B96DEA8C4E13F167948DCE38EB6A3972" bold="true" box="[189,328,1854,1879]" pageId="12" pageNumber="39">Etymology:</emphasis>
—Named after its
<taxonomicName id="4C194D1D4E13F1679627CE38E84C3972" box="[535,622,1854,1879]" class="Magnoliopsida" family="Fabaceae" genus="Glycine" kingdom="Plantae" order="Fabales" pageId="12" pageNumber="39" phylum="Tracheophyta" rank="genus">
<emphasis id="B96DEA8C4E13F1679627CE38E84C3972" box="[535,622,1854,1879]" italics="true" pageId="12" pageNumber="39">Glycine</emphasis>
</taxonomicName>
-inhabiting habit.
</paragraph>
<paragraph id="8BA6369E4E13F166948DCE64E8A23F24" blockId="12.[136,1452,1386,2059]" lastBlockId="13.[136,1452,160,257]" lastPageId="13" lastPageNumber="40" pageId="12" pageNumber="39">
<emphasis id="B96DEA8C4E13F167948DCE64EB2B395E" bold="true" box="[189,265,1890,1915]" pageId="12" pageNumber="39">Notes:</emphasis>
<taxonomicName id="4C194D1D4E13F1679516CE64E80E395E" authorityName="Cheew., Crous &amp; U. Braun" authorityYear="2015" box="[294,556,1890,1915]" class="Leotiomycetes" family="Dermateaceae" genus="Cercospora" kingdom="Fungi" order="Helotiales" pageId="12" pageNumber="39" phylum="Ascomycota" rank="species" species="glycinicola">
<emphasis id="B96DEA8C4E13F1679516CE64E80E395E" box="[294,556,1890,1915]" italics="true" pageId="12" pageNumber="39">Cercospora glycinicola</emphasis>
</taxonomicName>
is morphologically close to
<taxonomicName id="4C194D1D4E13F167975DCE64EE94395E" authority="(Shin &amp; Kim 2001)" baseAuthorityName="Shin &amp; Kim" baseAuthorityYear="2001" box="[877,1206,1890,1915]" class="Leotiomycetes" family="Dermateaceae" genus="Cercospora" kingdom="Fungi" order="Helotiales" pageId="12" pageNumber="39" phylum="Ascomycota" rank="species" species="sojina">
<emphasis id="B96DEA8C4E13F167975DCE64E9F0395E" box="[877,978,1890,1915]" italics="true" pageId="12" pageNumber="39">C. sojina</emphasis>
(
<bibRefCitation id="EF884B6F4E13F16797D1CE64EE8F395E" author="Shin, H. D. &amp; Kim, J. D." box="[993,1197,1890,1915]" pageId="12" pageNumber="39" refId="ref11926" refString="Shin, H. D. &amp; Kim, J. D. (2001) Cercospora and allied genera from Korea. Plant Pathology of Korea 7. National Institute of Agricultural Science and Technology, Suwon." type="journal volume" year="2001">Shin &amp; Kim 2001</bibRefCitation>
)
</taxonomicName>
but distinct in having shorter conidiophores (30113 μm,
<emphasis id="B96DEA8C4E13F1679629CE80E84339BA" box="[537,609,1926,1951]" italics="true" pageId="12" pageNumber="39">versus</emphasis>
40200 μm) and above all narrower conidia with few septa (35 μm wide, 13-septate,
<emphasis id="B96DEA8C4E13F1679527CEACEB7D39E6" box="[279,351,1962,1987]" italics="true" pageId="12" pageNumber="39">versus</emphasis>
48 μm wide, 37-septate). Phylogenetically it is also distinct from species presently known from DNA sequence data, including
<taxonomicName id="4C194D1D4E13F16795DBCEC8E87339C2" baseAuthorityName="Shin &amp; Kim" baseAuthorityYear="2001" box="[491,593,1998,2023]" class="Leotiomycetes" family="Dermateaceae" genus="Cercospora" kingdom="Fungi" order="Helotiales" pageId="12" pageNumber="39" phylum="Ascomycota" rank="species" species="sojina">
<emphasis id="B96DEA8C4E13F16795DBCEC8E87339C2" box="[491,593,1998,2023]" italics="true" pageId="12" pageNumber="39">C. sojina</emphasis>
</taxonomicName>
. However, although these two isolates originate from the same lesion, they were morphologically different. Isolate CPC 23911 had more geniculate conidiophores, shorter conidiogenous cells (2838 μm) and slightly shorter conidia (2368 μm). In contrast, isolate CPC 23912 had straight conidiophores, longer conidiogenous cells (4560 μm) and somewhat acicular conidia. However, phylogenetically the two isolates only differed via one nucleotide position in
<emphasis id="B96DEA8C4E12F1669609C9EEE85B3F24" box="[569,633,232,257]" italics="true" pageId="13" pageNumber="40">cmdA</emphasis>
.
</paragraph>
</subSubSection>
</treatment>
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