Toward a monograph of non-marine Ulvophyceae using an integrative approach (Molecular phylogeny and systematics of terrestrial Ulvophyceae II.) Author Darienko, Tatyana University of Göttingen, Experimental Phycology and Culture Collection of Algae, Nikolausberger Weg 18, D- 37073 Göttingen, Germany, and Kholodny Institute of Botany, National Academy of Science, Tereschenkivska Str. 2, Kyiv 01601, Ukraine Author Pröschold, Thomas University of Innsbruck, Research Institute for Limnology, Mondseestr. 9, A- 5310 Mondsee, Austria text Phytotaxa 2017 2017-10-06 324 1 1 41 http://dx.doi.org/10.11646/phytotaxa.324.1.1 journal article 10.11646/phytotaxa.324.1.1 1179-3163 13697485 Rhexinema edaphica Darienko, Pröschold et Lukešová sp. nov. ( Fig. 19A–G ) Diagnosis: Algae occurs as solitary cells, short 2–4-celled filaments or forms 2–4 packages surrounded by mucilage. The branching is very rudimentary and rare. Single cells are oval, and approximately 6.0–7.0 μm in diameter. Cells in packages are compressed from the sides, and are 5.0–10.0 μm long × 5.0–8.5 μm wide. Cells are uninucleate, with parietal chloroplasts, containing one good visible pyrenoid surrounded by several starch grains. Reproduction occurs by vegetative division. Zoospores are not observed. Other species of Rhexinema differ by a Chlorosarcinopsis- like morphology and by SSU-ITS sequences. Habitat: lateritic soil. FIGURE 19. Morphology of Rhexinema edaphica ULVO-10 grown on MiEB12 medium. A–E. cells packets and rudimentary branching, F,G. single cells and packets surrounded by common mucilage contrasted by indian ink. Type locality : Brazil , central part of Sao Paulo State , Sao Carlos, Itirapina and Pirissanunga County, elevation of about 800 m a.s.l., field of sugar cane, dense canopy, without any ground layer. Holotype (designated herein): The strain ULVO-10 is permanently cryopreserved in a metabolically inactive state (cryopreserved in liquid nitrogen) in the SAG , University of Göttingen , Germany . ITS-2 DNA Barcode: RHE 4 in Figs S2 .