Morphological and molecular identification for two new species of woodinhabiting macrofungi (Basidiomycota) from Yunnan-Guizhou Plateau, China Author Yang, Yang 0000-0001-8054-948X College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, P. R. China & fungiyoung @ 163. com; https: // orcid. org / 0000 - 0001 - 8054 - 948 X Author Li, Rong 0000-0003-1524-8247 Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, P. R. China & pearscus 0728 @ 163. com; https: // orcid. org / 0000 - 0003 - 1524 - 8247 Author Liu, Chaomao 0000-0003-2673-8890 College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, P. R. China & lcm 1987 swkx @ 163. com; https: // orcid. org / 0000 - 0003 - 2673 - 8890 Author Zhao, Changlin 0000-0002-8668-1075 College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, P. R. China & Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, P. R. China & fungichanglinz @ 163. com; https: // orcid. org / 0000 - 0002 - 8668 - 1075 text Phytotaxa 2023 2023-03-30 591 1 1 18 http://dx.doi.org/10.1094/PDIS-04-22-0755-PDN journal article 10.11646/phytotaxa.591.1.1 1179-3163 7784136 Coniophora yunnanensis Y. Yang & C.L. Zhao , sp. nov. Figs. 1 , 2 , 4 , 5 MycoBank no.: MB 847019 Holotype :— CHINA . Yunnan Province , Pu’er , Jingdong County , Wuliangshan National Nature Reserve , E 100°31′10′′ , N 24°25′07′′ , elev. 1500 m , on the dead bamboo, 2 October 2017 , CLZhao 3594 ( SWFC !), GenBank No. (ITS OP901837; nLSU OP904196). FIGURE 1. Maximum Parsimony strict consensus tree illustrating the phylogeny of two new species and related species inAgaricomycetidae based on ITS+nLSU sequences. Branches are labeled with maximum likelihood bootstrap values equal to or above 70%, parsimony bootstrap values equal to or above 50% and Bayesian posterior probabilities equal to or above 0.95. The new species are in bold. FIGURE 2. Maximum Parsimony strict consensus tree illustrating the phylogeny of the new species of Coniophora based on ITS sequences. Branches are labeled with maximum likelihood bootstrap values equal to or above 70%, parsimony bootstrap values equal to or above 50% and Bayesian posterior probabilities equal to or above 0.95. FIGURE 3. Maximum Parsimony strict consensus tree illustrating the phylogeny of the new species of Resupinatus based on ITS sequences. Branches are labeled with maximum likelihood bootstrap values equal to or above 70%, parsimony bootstrap values equal to or above 50% and Bayesian posterior probabilities equal to or above 0.95. Etymology: — yunnanensis (Lat.) : refers to the locality ( Yunnan Province ) of the type specimen. Basidiomata : —Annual, resupinate to effused, separable, soft ceraceous, without odor or taste when fresh, becoming hard corky upon drying, up to 10 cm long, 4 cm wide, 0.5–1 mm thick; hymenial surface odontoid, 100–500 µm long, olivaceous buff (4C4) when fresh, olivaceous buff (4C4) to greyish brown (5/6E4) drying; sterile margin indistinct and olivaceous buff (4C4). Hyphal structure : — Hyphal system monomitic; generative hyphae with simple septa, colorless, thin-walled, rarely branched, interwoven, 2.5–6 μm in diam; IKI–, CB–, tissues unchanged in KOH. Hymenium : — Cystidia and cystidioles absent; basidia cylindrical, constricted, 40–90 × 5–7 µm, with 4-sterigmata and a basal simple septum, basidioles dominant, in shape similar to basidia, but slightly smaller. Basidiospores : — Ovoid to broadly ellipsoid, smooth, thick-walled, pale yellowish, with oil drops, IKI–, CB–, (8–)8.5–12.5 × (5.5–)6.5–8.5 µm, L = 10.7 µm, W = 7.27 µm, Q = 1.46–1.53 (n = 60/2), Qm = 1.47. Additional specimen examined : — CHINA . Yunnan Province , Pu’er , Jingdong County , Wuliangshan National Nature Reserve , E 100°31′10′′ , N 24°25′07′′ , elev. 1500 m , on the dead bamboo, 2 October 2017 , CLZhao 3517 ( SWFC !), GenBank No. (ITS OP901836; nLSU OP904195) . Notes: Morphologically, Coniophora arida (Fr.) P. Karst. (1868: 370) , C. hanoiensis Pat. (1907: 76) , C. olivacea (Fr.) P. Karst. (1879: 162) , and C. puteana (Schumach.) P. Karst. (1803: 397) are similar to C. yunnanensis by having ovoid to ellipsoid basidiospores. However, C. arida differs from C. yunnanensis by its wider basidia (40–70 × 7–10 µm), and presence of the hyphal strands ( Bernicchia & Gorjón 2010 ); C. hanoiensis is separated from C. yunnanensis by its white to yellowish-brown hymenial surface and dimitic hyphal system ( Ginns 1982 ); C. olivacea is distinguished from C. yunnanensis by smooth hymenial surface, presence of the thick-walled septate cystidia and dextrinoid basidiospores ( Bernicchia & Gorjón 2010 ); C. puteana is separated from C. yunnanensis by having the tabular, yellowish-brown, smooth basidiomata and brownish basidiospores ( Bernicchia & Gorjón 2010 ). Coniophora yunnanensis resembles C. arachnoidea , C. fusispora (Cooke & Ellis) Cooke (1889: 650) and C. ladoi Tellería (1991: 236) in having a odontoid hymenial surface. However, Coniophora arachnoidea is different from C. yunnanensis by brownish hymenophore and smaller basidiospores (6–8 × 4–5 µm) ( Blanco et al. 2009 ); C. fusispora can be delimited from C. yunnanensis by its orange-yellow to brown hymenophore and longer basidiospores (14–20 × 5–8 µm) ( Bernicchia & Gorjón 2010 ); C. ladoi is distinguished from C. yunnanensis by small basidiospores (5.5–7.5 × 3.5–4.5 µm) ( Bernicchia & Gorjón 2010 ).