Euglossa marianae sp. n. (Hymenoptera: Apidae): a new orchid bee from the Brazilian Atlantic Forest and the possible first documented local extinction of a forest-dependent orchid bee Author Nemésio, André text Zootaxa 2011 2892 59 68 journal article 46821 10.5281/zenodo.277663 fdf964de-f5be-42c5-9f1f-eab2ba5e6345 1175-5326 277663 Euglossa (Euglossa) marianae Nemésio , sp. n. Diagnosis. This species is clearly assigned to the subgenus Euglossa (Euglossa) Latreille, 1802 due to its small size, short extended tongue, rhomboid metatibia, small and widely separated tufts on S2. It is also easily included within Euglossa analis group due to the throughout dark blue to violet coloration, except in the last three terga, which are green or reddish depending on the species. In Euglossa marianae sp. n. the three last terga are bright green ( Figure 2 C). Nevertheless, it can be distinguished from Euglossa analis , its most closely related species, by the shape of the anterior mesotibial tuft (see Fig. 2 E–F). In Euglossa analis , the anterior tuft is divided in two lobes, the inferior one much larger than the superior lobe (see Fig. 2 F and also this character in the holotype , illustrated in Nemésio 2009 : 103). In Euglossa marianae sp. n. the two lobes are approximately the same size, the superior lobe slightly pointed ( Fig. 2 E). The other species in Euglossa analis group occurring in the Atlantic Forest domain present the last three terga reddish colored and cannot be confused with E. marianae sp. n. It can be also differentiated from the Amazonian Euglossa retroviridis Dressler, 1982a , the only other species in the group which also have the last three terga green-colored, by the shape of mesotibial tufts (see drawings in Dressler, 1982a ) and by having only two teeth in the mandible ( E. retroviridis males have three teeth). Description (Male, Fig. 2 ) Color and vestiture. Clypeus violet, rest of head dark blue to violet ( Fig. 2 B); mesosoma dark blue to violet ( Fig. 2 A); T1–T4 solid violet, T5–T7 bright green ( Fig. 2 A, C). Wings pale brown. Pubescence very sparse, predominantly fulvous hairs on metasoma and antennal sockets, black and fulvous hairs on mesosoma, black hairs especially on scutum (compared to predominantly fulvous hairs in Eg. analis ). Ivory paraocular markings well developed, not reaching malar area; anterior surface of antennal scape black. Head. Width 4.7 mm; interorbital distance at level of antennal sockets 2.7 mm; maximum interorbital distance 3.0 mm; scape 1.1 mm; eye length 2.9 mm. Body. Body length ca. 11.0 mm; anterior wing ca. 8.9 mm; tongue in repose reaching hindcoxa; scutellum 2.8 mm wide and 1.3 mm long; abdominal width 4.5 mm. Legs. Foretibia and forebasitarsus fringed with medium-sized, dense, fulvous hairs; velvet area occupying all the ventral side of mesotibia, posterior mesotibial tuft small, oblong; anterior mesotibial tuft deeply notched, with two lobes of approximately the same size (in Eg. analis the inferior lobe much larger than the superior lobe – see Fig. 2 F), upper lobe slightly pointed ( Fig. 2 E); metatibia oblong-rhomboid, inflated, post-glandular area fringed with medium-sized hairs ( Fig. 2 D). Metasoma. Punctation on discal base of T1 sparse, with large circular punctures; on distal part of T1 and T2- T4 dense, comprised of small circular punctures; on T5-T7 dense, with large circular punctures. S2 with small, widely separated tufts. Etymology. The specific epithet honors Mariana Abrahão Assunção, student of Biological Sciences at the Universidade Federal de Uberlândia, Minas Gerais. Type locality. Holotype collected at Parque Estadual do Rio Doce ( 19º43’S - 42º34’W ; 200 m a.s.l.), in the municipality of Marliéria, state of Minas Gerais, southeastern Brazil . Attractive baits. Specimens of this species have been collected at cineole and skatole baits, some with orchid pollinaria of Coryanthes attached (Dressler’s field notes). Female. Unknown. Type material: HOLOTYPE – male, with the following data: “ Euglossini do PERD , Pq. E. Rio Doce, 3858- 11096” and “ Brasil , Marliéria, MG, 04/07/1999 , A. Nemésio” and “ Euglossa (Euglossa) marianae Nemésio , sp. n. , HOLOTYPUS ” ( UFMG ). PARATYPES – 30 males , with the same label data: “ Brasil , MG, Marliéria, Pq. E. Rio Doce, Junho/1999, A. Nemésio leg .” ( UFMG – to be distributed to other institutions).