A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene urbica Schubart, 1945 Figs 18A , 140– 142 , 164O , 166P , 173E , 189 ; Supp. file 4: Fig. 223B Pseudonannolene urbica Schubart, 1945a: 313 , figs 13–14. Pseudonannolene urbica – Jeekel 2004: 92 . — Gallo & Bichuette 2020: 36 . Diagnosis Males of P. urbica differ from all congeners by having the telopodite and internal branch short (less than ⅓ of gonocoxa in length) ( Fig. 141D–F ); solenomere short and trianguliform; apicomesal process located medially ( Figs 141D , 223B ). Etymology Although unspecified, the name is evidently related to the Latin adjective ‘ urbicus ’ (feminine ‘ urbica ’) = ‘related to city’, referring to the occurrence of the species in urban areas. Material examined Holotype BRAZIL • ♂ [gonopods, gnathochilarium, first and second leg-pair on microscope slides]; São Paulo , São Paulo , Jardim do Museu de Zoologia [MZSP], Ipiranga ; [ -23.585105 , -46.600998 ]; 790 m a.s.l. ; 3 Mar. 1943 ; F. Lane leg.; MZSP . Paratypes (total: 1 ♂, 1 immature) BRAZIL • 1 ♂, 1 ♂ immature; same collection data as for holotype; MZSP . Other material (total: 9 ♂♂ , 4 ♀♀ , 1 immature ) BRAZIL – São Paulo • 1 ♀ ; São Paulo , Parque dos Príncipes ; [ -23.572951 , -46.772286 ]; 794 m a.s.l. ; 14 Mar. 2003 ; IBSP 7887 • 1 ♂ ; São Paulo ; [ -23.550439 , -46.633317 ]; 769 m a.s.l. ; IBSP 2007 • 1 ♂ , 1 ♀ ; same collection data as for preceding; IBSP 2008 • 1 ♂ ; campus USP , Mata do Cuaso ; [ -23.561075 , -46.724394 ]; 730 m a.s.l. ; 12–19 Dec. 1999 ; D.F. Candiani leg.; IBSP 1249 • 1 ♂ ; Mata da Previdência ; [ -23.571120 , -46.709728 ]; 732 m a.s.l. ; 22 Feb. 2001 ; F.S. Cunha leg.; IBSP 716 • 1 ♂ ; Bairro Ipiranga ; [ -23.530503 , -46.666090 ]; 744 m a.s.l. ; 31 Jan. 1944 ; O. Schubart leg.; MZSP • 1 ♀ ; Bairro Morumbi ; [ -23.598485 , -46.720072 ]; 746 m a.s.l. ; 6 Dec. 1948 ; E. Marcus leg.; MZSP • 3 ♂♂ , 1 ♀ ; Eldorado ; [ -23.709807 , -46.627225 ]; 785 m a.s.l. ; 1 Nov. 1947 ; E. Marcus leg.; MZSP • 1 ♂ immature; Mogi das Cruzes , Jundiapeba , Parque São Martinho ; [ -23.614280 , -46.236089 ]; 814 m a.s.l. ; 6–7 Jan. 2017 ; R.S. Bouzan leg.; IBSP 7886 • 1 ♂ ; Amparo , Fazenda São Bento ; [ -22.708011 , -46.772597 ]; 687 m a.s.l. ; 8 Mar. 1943 ; F. Lane leg.; MZSP . Descriptive notes MEASUREMENTS . 50–53 body rings (1–2 apodous + telson). Males: body length 47.6–50.2 mm ; maximum midbody diameter 2.5–2.7 mm . Females: body length 52–53 mm ; maximum midbody diameter 2.8–3 mm . COLOR . Body color brownish grey; head, collum, antennae darker; prozonites anteriorly greyish; metazonites with a posterior band lighter; legs brownish. HEAD . Antennae short ( Fig. 164O ), just reaching back to end of ring 5 when extended dorsally; relative antennomere lengths 1<2<3>4>5≈6>7. Mandibular cardo with ventral margin narrow. Ommatidial cluster well-developed, elliptical; ca 41 ommatidia in 6 rows. BODY RINGS . Collum with lateral lobes rounded, with ca 9 striae, slightly curved ectad ( Fig. 140A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae below ozopore. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 173E ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) short (less than half of remaining podomere lengths), subtriangular, with the base arched, densely setose ( Fig. 141A ); prefemoral process ( prf ) about as wide as half of prefemur, subcylindrical, curved ectad, densely setose up to its median region ( Fig. 141B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) rounded; penis ( pn ) located at proximal region, rounded, not extended basally ( Fig. 141C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) rounded, with the base arched; antero-posteriorly flattened ( Fig. 141D– F ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder ( sh ) rounded. Telopodite ( tp ) shorter than ⅓ of gcx ( Fig. 141D ); solenomere ( sl ) with short squamous region; apicomesal process ( amp ) subtriangular, located medially, with sa also at medial portion and visible apically; ectal process absent. Internal branch ( ib ) short, subtriangular, surrounding basally tp as a shield; ib with setae along its entire margin, but only with the apical setae exceeding seminal region of sl ( Figs 141D–F , 223B ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous; internal valve subtriangular; operculum large, curved ectad; external valve wide, subtriangular. Distribution The species has been recorded only in the metropolitan region of the Brazilian city of São Paulo and surrounding area ( Fig. 189 ).