Infundibulicybe swatica (Basidiomycota, Agaricales), a new species from Pakistan Author Kiran, Munazza 0000-0002-3671-9039 Department of Botany, Division of Science & Technology, University of Education, Lahore 54000, Pakistan & Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan munazza.kiran@ue.edu.pk Author Amjad, Atika 0000-0002-9688-1361 Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan atikaamjad9@gmail.com Author Naseer, Arooj 0000-0002-4458-9043 Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan arooj.hons@pu.edu.pk Author Ullah, Zia 0000-0002-0806-2375 Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan genetics3333@gmail.com Author Razzaq, Annum 0000-0002-3593-5821 Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan annum.phd.botany@pu.edu.pk Author Hussain, Shah 0000-0002-5772-7206 Centre for Environmental Studies and Research, Sultan Qaboos University, P. O. Box 34, Al-Khoud 123, Sultanate of Oman & Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, PO Box 34, Al-Khoud 123, Sultanate of Oman shahpk85@gmail.com Author Khalid, Abdul Nasir 0000-0002-5635-8031 Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan drankhalid@gmail.com text Phytotaxa 2024 2024-11-19 672 1 89 98 https://doi.org/10.11646/phytotaxa.672.1.6 journal article 10.11646/phytotaxa.672.1.6 1179-3163 14521527 Infundibulicybe swatica Amjad, Kiran & Khalid , sp.nov. ( Figs. 3 & 4 ) MycoBank: —MB842483 FIGURE 3. A–D Basidiomata of Infundibulicybe swatica in the field. Scale bars: A–D =15mm. FIGURE 4. A–D Line drawings of Infundibulicybe swatica . A—basidia, B—basidiospores, C—pileipellis and D— stipitipellis. Scale bars: A=B= 5 µm, C= 20 µm, D =15 µm. Etymology: —‘ swatica’ represents the type locality from which it was collected. Diagnosis: — Infundibulicybe swatica differs macro-morphologically from its closest ally Infundibulicybe gibba by pale yellow to pale yellow brown to brown pileus with a dark brown center and bicolorous stipe: white at the apex and pale yellow brown at the base, in contrast to the pinkish tan pileus and white to off-white slightly paler stipe of I.gibba . Anatomically, both species have smooth, lacrymoid to sub-lacrymoid basidiospores but the latter species has longer size of basidiospores (≥ 7 μm). “ Type : — PAKISTAN . Khyber Pakhtunkhwa Province : Swat District, Sakhra, on soil under mixed coniferous and Quercus trees, 35º 4ʹ 38ʺ North and 72º 24ʹ 48ʺ East 1,480 m , 15 Aug 2019 , Zia Ullah, SK-11 (LAH37008!, ITS: OM203466)”. Description: — Basidiomata small to medium-sized. Pileus 2–7 cm in diameter, umbilicate when young, becoming slightly infundibuliform at maturity, deeply depressed when mature, pale yellow (10YR 8/6) to pale yellow brown (10YR 6/8) to brown (7.5YR 6/6) pileus with a dark brown center (10YR 5/8), surface dry, margins involute, rarely revolute when young, sometimes incised at maturity. Lamellae decurrent, crowded to close, thin, narrow, sometimes forked, cream colored (2.5GY 9/2). Lamellulae present in a single tier. Stipe 5–6 cm long, central or sub-eccentric, equal or sometimes with a slightly enlarged base often with white tomentum adhering to leaves or woody debris, glabrous or slightly longitudinally striated, bicolorous, white at the apex, concolorous to that of pileus at the base. Basidiospores [n/b/p] [60/6/6], (4.0) 4.5 8.5(̶ 9) × (2.0 ̶) 3.5 5.0 (̶ 5.5) µm, avl × avw = 6.5 × 4.2 µm, Q = (1.1‒) 1.2‒2.1 (‒2.8), Qe = 1.6, lacrymoid to sub-lacrymoid, smooth, hyaline, apiculate, inamyloid. Basidia 26 36.5 × 6 8.5 µm (avl × avw = 31 × 7 µm), narrowly clavate, hyaline in KOH, clamped, 2–4 spored, sterigmata 2 2.5 µm long. Hymenial cystidia absent. Pileipellis intricate trichoderm, hyphae septate, cylindrical, 7 13 µm in dia. (av = 10.5 µm). Stipitipellis intricate trichoderm, hyphae septate, cylindrical, interwoven, 3.5 9.5 µm (av = 7.5 µm) in diameter, hyaline in KOH. Clamp connections present in all tissues. Additional materials examined : PAKISTAN . Khyber Pakhtunkhwa Province : Swat District , Sakhra, 1680 m , on soil, 15 August 2019 , Zia Ullah SK 26 ( LAH37009 , ITS : OM203467 ), Shawar Valley , 2100 m , solitary on soil, 28 July 2016 , Zia Ullah, AR-09 ( LAH37129 , ITS : ON527812 , LSU : ON527816 ), AR-43 ( LAH37130 , ITS : ON527813 ), solitary on soil under Quercus incana (Bartram,W., Travels Carolina 1791) , 14 July 2015 , A . Naseer, M . Kiran & A . N . Khalid, SW-35 ( LAH35235 , ITS : ON527814 ) & SW-86 ( LAH35236 , ITS : ON527815 , LSU : ON527817 ). Punjab province : Rawalpindi District , Bhurban town ; 1828 m , on soil under Pinus ; 7 September 2020 , Annum Razzaq BR56 ( ITS : PP345592 ), GC26 ( ITS : PP345593 ), GC25 ( ITS : PP345594 ) .