A new species of Zoraptera, Zorotypus komatsui sp. nov. from Cameroon and a redescription of Zorotypus vinsoni Paulian, 1951 (Polyneoptera, Zoraptera) Author Matsumura, Yoko https://orcid.org/0000-0002-3438-2161 Systematic Entomology, Department of Ecology and Systematics, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan yoko.matumura.hamupeni@gmail.com Author Maruyama, Munetoshi https://orcid.org/0000-0003-4531-1008 The Kyushu University Museum, Hakozaki 6 - 10 - 1, Fukuoka, 812 - 8581, Japan Author Ntonifor, Nelson N. Department of Agronomic and Applied Molecular Sciences, Faculty of Agriculture and Veterinary Medicine, University of Buea, P. O. Box 63 Buea, Cameroon Author Beutel, Rolf G. https://orcid.org/0000-0002-0433-7626 Entomology Group, Institut fuer Zoologie und Evolutionsforschung, Friedrich-Schiller-Universitaet Jena, Jena, Germany text ZooKeys 2023 2023-09-01 1178 39 59 http://dx.doi.org/10.3897/zookeys.1178.108276 journal article http://dx.doi.org/10.3897/zookeys.1178.108276 1313-2970-1178-39 2F94A6DC2CBC4B76BBD178FFB8909235 7893A52D5D7959C9A1916016B97CB36F Zorotypus komatsui Matsumura, Maruyama, Ntonifor & Beutel sp. nov. Figs 1 , 2 , 3 , 4 Zorotypus sp. 1 cameroon YK2: Matsumura et al. 2020 : 352. Type material. Holotype . Cameroon: apterous male, South-west region, Nyasoso, Mt Kupe, 4°50'12.5"N , 9°41'21.7"E , 16.v.2015, coll. Takashi Komatsu (depository number/ SEHU48817-48818, parts of antennae mounted on another glass slide). The male was found under a rock which is unusual for Zoraptera (see Remarks for detail). Diagnosis. Males can be easily distinguished from those of other zorapteran species from Africa by the prominently developed projections on Tx and Txi and two pairs of sensilla basiconica on Tx. The following features should be added to the diagnosis: (1) eye spots absent in apterous males; (2) antennomere ii shorter than antennomere iii; (3) posterior metafemural surface covered with seven stout spiniform bristles, two of which are distantly located on middle region and longer, and the rest arranged evenly on the proximal portion; and (4) metatibia with three stout spiniform bristles, two of them inserted apically. Description. Apterous male. Measurements . N = 1. Total body length: 2.42 mm, head width 0.50 mm, head length 0.47 mm, antennal length 1.26 mm, pronotal length 0.38 mm, metafemural length 0.68 mm, metatibial length 0.79 mm, abdominal maximum width 0.49 mm, length of cerci 0.11 mm. Color . Coloration light brown except for membranous regions and less pigmented cerci, antennae, and legs (Fig. 1 ). The holotype was processed with Proteinase K (Qiagen, Tokyo, Japan) and the body consequently less pigmented than in a natural condition. Figure 1. Digitalmicroscopic images of the holotype of Zorotypus komatsui sp. nov. (ventral view) A habitus B head and prothorax C hindleg and abdomen. Head (Figs 1 , 2A ). Head subtriangular, without black eye spots (Figs 1 , 2A ); ocelli absent; cephalic chaetotaxy as in Fig. 2A ; relatively long sinuate setae densely arranged on vertex, referred to as fontanelle (e.g., Delamare-Deboutteville 1951 ; van Ryn-Tournel 1971 ) (Fig. 2A ). Antennae 9-segmented; antennomere i slightly curved outward, longer than wide; antennomere ii short, about 1/3 as long as antennomere i, longer than antennomere iii (Fig. 1B ); antennomeres iv-ix cylindrical and longer than wide, about 2.2 times as long as antennomere ii. Labial palps three-segmented. Figure 2. Adult Zorotypus komatsui sp. nov. male A head, dorsal view B thorax, dorsal view C abdomen, dorsal (left) and ventral (right) views. Abbreviations: ce; cercus, cx; coxa, sb; sensilla basiconica. Thorax (Figs 1 , 2B ). Pronotum trapezoid. Mesonotum trapezoid, as long as pronotum. Metanotum trapezoid, distinctly wider than long, shorter than mesonotum. Thoracic setation as in Fig. 2B . Legs covered with short and moderately long setae; tarsi 2-segmented and covered with moderately long setae, with small unguitractor plate apically and pair of claws; posterior and ventral profemoral surfaces with moderately long setae; protibia with moderately long setae, and bristles arranged as comb on distal two-thirds along ventral surface; anterior and ventral mesofemural surfaces with moderately long setae; mesotibia with vestiture of moderately long setae and two apical spurs; metafemur broader than profemur, wider proximally than distally; ventral metafemural surface covered with moderately long setae; posterior surfaces with seven stout spiniform bristles, two on middle region, rest evenly distributed distally and longer than central ones (Fig. 1C ); metatibia with moderately long setae and three stout spiniform bristles, two of them inserted apically (Fig. 1C ). Abdomen (Figs 1 , 2C , 3 , 4 ). Chaetotaxy of abdominal tergites and sternites as in Fig. 2C . Abdominal tergum 1 (Ti) with single transverse row of short setae; Tii-vi with two transverse rows of short setae and additional setae of moderate length; Tvii with three transverse rows of moderately long setae; posterior two-thirds of Tviii covered with moderately long setae and several long setae along posterior edge; Tix trapezoidal, posteriorly narrow, with row of long setae along posterior edge; Tx with two pairs of sensilla basiconica (Fig. 2C ), medially heavily sclerotized and continuing as bifurcated projection (Fig. 3 ); Txi partially sclerotized but partially semimembranous, with sclerotized median projection originating from left side (Fig. 3D-E '). Projections on Tx and Txi very large, superficially resembling asymmetric male genitalia; projection on Tx bifurcated, fork-like; projection on Txi triangular, with horn on dorsal side (Fig. 3E asterisk). Cerci unsegmented, conical with numerous short setae and several long and fine setae. Si scarcely sclerotized; Sii laterally weakly sclerotized; Siii with transverse row of short setae along posterior margin; Siv with two transverse rows of short setae and additionally setae of moderate length; Sv-vii with three transverse rows of short setae and setae of moderate length; Sviii wider than long, with moderately long setae evenly distributed except for anterior and middle regions; posterior margin bifurcated, tips bent dorsad (Fig. 3C, C '). Genitalia with six inconspicuous sclerites (Fig. 4 , the largest sclerite with microstructures [Fig. 4 , black arrowhead] and pointed protuberance [Fig. 4 , white arrowheads]), one globular less sclerotized structure (Fig. 4 , white arrows) and two membranous projections (Fig. 4 , asterisks); highly reduced, almost vestigial (Fig. 4 ). Figure 3. Apical region of male abdomen of Zorotypus komatsui sp. nov. A-E micrographs A'-E ' corresponding drawings A-A ' dorsal view B-B ' ventral view C-C ' lateral view D-D ' caudal view E-E ' caudodorsal view. Abbreviations: an; anus, ce; cercus, ge; genitalia, sb; sensilla basiconica. Figure 4. Male genitalia of Zorotypus komatsui sp. nov. with six inconspicuous sclerites, one globular less sclerotized structure (arrows) and two membranous projections (asterisks) A-D light micrographs E CLSM images (white arrowhead denotes a projection, and the black one denotes microstructures) F schema showing positions of six sclerites A, D ventral view B, C, E lateral view D lateral view, the sclerites were traced on E . Distribution. Cameroon, South-west region, Nyasoso, Mt Kupe. Remarks. The habitat of the individual we obtained was unusual for a zorapteran species. The male was found under a rock ca 30 cm long and half embedded in soil. Furthermore, the rock was located in an open relatively dry area. The collector of the specimen T. Komatsu and one of the authors (MM) tried intensively to find zorapterans in rain forests nearby, but no additional individual was found.