First blind daddy long-legs spiders from Australia and Reunion (Araneae, Pholcidae) Author Huber, Bernhard A. https://orcid.org/0000-0002-7566-5424 Zoological Research Museum Alexander Koenig, LIB, Bonn, Germany b.huber@leibniz-zfmk.de Author Meng, Guanliang Zoological Research Museum Alexander Koenig, LIB, Bonn, Germany Author Clark, Huon L. Bennelongia Environmental Consultants, 5 Bishop Street, Jolimont WA 6014, Australia & Collections and Research, Western Australian Museum, 49 Kew Street, Welshpool, Western Australia 6106, Australia Author Cazanove, Gregory Museum d'Histoire Naturelle, Saint Denis, Reunion text Subterranean Biology 2023 2023-07-24 46 1 19 http://dx.doi.org/10.3897/subtbiol.46.105798 journal article http://dx.doi.org/10.3897/subtbiol.46.105798 1314-2615-46-1 1A679D4370004DD1BA0D69DA2057401E 73A944AECDDB5130BA73E57C7CB6D83F Belisana coblynau Huber & Clark sp. nov. Figs 1 , 2 Type material. Australia - Western Australia • ♂ holotype; ~85 km ESE Pannawonica; 21.8836°S , 117.1211°E ; 590 m a.s.l.; 25 Jun. 2019; M.D. Scanlon and H.L. Clark leg.; WAM T158896. Type locality. Australia, Western Australia, ~85 km ESE Pannawonica, 21.8836°S , 117.1211°E , 590 m a.s.l. Type specimen. Holotype male, in ethanol. Original labels: "WA: JSE, ca 87 km ESE Pannawonica, 21°53'43.450"S , 117°07'48.63"E (GDA 94) 25. Jun. 2019, Scanlon MD, Clark HL (KRC 0364), Trog scrape, 35 m", "KRC0364" , " WAM T 158896", "100% EtOH". Other material examined. Australia - Western Australia • 1 ♀ , assigned tentatively, see Remark below; same locality as holotype but 3.7 km NW; 21.8583°S , 117.0972°E ; 540 m a.s.l. ; 25 Apr. 2019 ; M.D. Scanlon and H.L. Clark leg.; "KRC0226" , WAM T158897 • 1 juv. ; same locality as holotype but 1.6 km SE; 21.8954°S , 117.1302°E ; 570 m a.s.l. ; 26 Apr. 2019 ; M.D. Scanlon and H.L. Clark leg.; "KRC0254" , WAM T158895 . Remark. The genetic distance (Table 1 ) between the male holotype and the juvenile specimen was 0.5%, strongly suggesting that the two are conspecific. The distances between these two specimens and the female was much higher, at 8.5 and 8.8%, respectively. This is within a range that in Pholcidae is always problematic due to the overlap of intra- and interspecific genetic (CO1) variation between approximately 8 and 11% ( Astrin et al. 2006 ; Huber et al. in press ). In the case of the female, we are thus unsure about its taxonomic status. Table 1. GenBank accession numbers and genetic distances. CO1 accession numbers of the three Belisana coblynau specimens and genetic distances (K2P) among them and other Belisana species taken from Eberle et al. (2018) .

Taxon	OQ525972	OQ525971	OQ525973
OQ525972, Belisana coblynau male	-	-	-
OQ525971, Belisana coblynau female	0.088	-	-
OQ525973, Belisana coblynau juvenile	0.005	0.085	-
S127_Belisana_Bor152_Bor166	0.277	0.261	0.277
S249_Belisana_Mal91_Mal290	0.285	0.283	0.287
S242_Belisana_leuser_Mal304	0.233	0.241	0.233
S247_Belisana_Mal76_Mal278	0.274	0.299	0.276
S498_Belisana_minneriya_SL123	0.238	0.245	0.236
S248_Belisana_Mal77_Mal279	0.277	0.274	0.275
S103_Belisana_Tai4_Tai67	0.233	0.264	0.231
S230_Belisana_australis_Phi280	0.244	0.264	0.244
S125_Belisana_Bor121_Bor198	0.227	0.241	0.225
S244_Belisana_Mal30_Mal241	0.255	0.269	0.259
S333_Belisana_bohorok_Ind127	0.250	0.250	0.248
S336_Belisana_nahtanoj_Ind181	0.209	0.212	0.208
S338_Belisana_tambligan_Ind213	0.237	0.234	0.234
S339_Buitinga_buhoma_Uga124	0.254	0.269	0.251
S340_Buitinga_ruhiza_Uga122	0.252	0.262	0.247
S341_Buitinga_ruwenzori_Uga156	0.255	0.269	0.249
S334_Belisana_Ind15_Ind140	0.274	0.256	0.274
S386_Belisana_sabah_Bor214	0.235	0.250	0.233
S417_Belisana_ranong_Mal361	0.210	0.215	0.210

Diagnosis. Distinguished from known congeners (and other Pholcinae ) by details of procursus (Fig. 2D, E ; straight, with simple retrolateral process, bifid prolateral process, and widened membranous tip), genital bulb (Fig. 2A, B ; bulbal apophysis short and hooked, embolus straight and pointed at tip), male chelicerae (Fig. 2C ; pair of light proximal processes; pair of simple distal frontal apophyses without modified hairs), epigynum (Fig. 1B ; protruding, with pair of pockets close together), and female internal genitalia (Figs 1C, D , 2F ; pore plates wide apart; distinctive pair of additional internal lateral pockets). Figure 1. Belisana coblynau Huber & Clark, sp. nov., male holotype and female, WAM T158896-7 A male habitus, lateral view B female abdomen, ventral view C, D cleared female genitalia, ventral and dorsal views. Abbreviations: ep, epigynal pockets; lp, lateral pocket; pp, pore plate; sp, spinnerets. Scale bars: 0.5 mm ( A ); 0.1 mm ( B-D ). Figure 2. Belisana coblynau Huber & Clark, sp. nov., male holotype and female, WAM T158896-7 A, B left male palp, prolateral and retrolateral views C male chelicerae, frontal view D, E left procursus, prolateral and retrolateral views F cleared female genitalia, dorsal view. Abbreviations: b, genital bulb; ba, bulbal apophysis; c, coxa; e, embolus; ep, epigynal pocket; f, femur; lp, lateral pocket; pp, pore plate; pr, procursus; ta, tarsus; ti, tibia. Scale bars: 0.2 mm ( A, B, F ); 0.1 mm ( C-E ). Description. Male (holotype). Measurements . Total body length ~1.5-1.6 (abdomen detached), carapace width 0.60. Leg 1: 11.5 (2.9 + 0.2 + 3.0 + 4.4 + 1.0); legs 2 and 3 missing; tibia 4: 2.3; tibia 1 L/d: 50. Color (in ethanol). Entire specimen pale ochre to whitish (Fig. 1A ). Body . Habitus as in Fig. 1A . Ocular area not raised, without any remnants of eye pigment and lenses. Without thoracic groove. Clypeus unmodified. Sternum wider than long (0.40/0.30), unmodified. Abdomen globular, conical at spinnerets. Chelicerae . As in Fig. 2C , with pair of light proximal processes, pair of simple distal frontal apophyses without modified hairs (distance between tips: 50 µm ), without stridulatory files. Palps . As in Fig. 2A, B ; coxa unmodified, trochanter with low rounded retrolateral ventral protrusion, femur cylindrical, without process; tibia with only one (retrolateral distal) trichobothrium; procursus (Fig. 2D, E ) straight, with simple retrolateral process, bifid prolateral process, and widened membranous tip; genital bulb with short hooked bulbal apophysis and straight embolus pointed at tip. Legs . Apparently without spines and curved hairs (most hairs on available legs missing); leg trichobothria not seen; tarsus 1 with ~10 pseudosegments, barely visible in dissecting microscope. Female. In general similar to male, but larger (in particular the abdomen); total body length 1.8; carapace width 0.62; most legs detached (tibia 4: 2.6). Epigynum (Fig. 1B ) main anterior plate semicircular, with distinct pair of pockets (distance between pockets: 30 µm ), in lateral view protruding. Internal genitalia (Figs 1C, D , 2F ) with pair of pore plates wide apart, distinctive pair of additional internal lateral pockets. Each ALS with 3-4 small cylindrical spigots in addition to large widened spigot and long pointed spigot (checked in cleared abdomen). Etymology. The species epithet refers to the mythical gnome-like creatures that are said to haunt mines and quarries (of Wales and beyond); noun in apposition. Distribution. Known from three neighboring (within 5 km) localities in Western Australia. Natural history. The spiders were extracted from mining bores in the Hamersley Range using scrape sampling (Fig. 5A-C ). This collecting method precludes gaining detailed natural history data. The specimens were collected between ground level and approximately 35 m below ground level. The temperature in these holes at 15 m below ground level was constant at 32-33 °C. For a general characterization of the Pilbara troglofauna, see Discussion.