A new striking dendrobatid frog (Dendrobatidae: Aromobatinae, Aromobates) from the Venezuelan Andes Author Barrio-Amorós, César L. Author Rivero, Ramón Author Santos, Juan C. text Zootaxa 2011 3063 39 52 journal article 46151 10.5281/zenodo.201877 0feb05fc-8711-4ec7-a1fe-2e5f9d80357d 1175-5326 201877 Aromobates ornatissimus sp. nov English name: Ornate cloud frog Spanish name: Sapito de niebla adornado Holotype . Adult male, EBRG 5292, from Las Palmas, Municipio Carache, Estado Trujillo, Venezuela , 09º41’47’’N , 70º08’24’’W ( 9.6964 N – 70.1400 W ); elevation 2350 m , collected by Ramón Rivero the 4th of April, 2005. Paratopotypes. Six adult males ( EBRG 5285, 5287, 5290-94) and five adult females ( EBRG 5282, 5288-89, 5298-99), all with the same data as the holotype . EBRG 6146-47, and CVULA 8351, collected by Ramón Rivero and César L. Barrio-Amorós, the 18th March 2011 . Referred specimens. Four juveniles ( EBRG 5284, 5286, 5295, 5297), all with same data as the holotype . Tadpoles. Unknown. Diagnosis. (1) Skin on dorsum smooth (with notable tubercles posteriorly in life). (2) Paired dorsal scutes present on digits. (3) Distal tubercle on finger IV present. (4) Finger IV length reaches distal half of subarticular tubercle of finger III. (5) Finger I longer than II. (6) Digital discs present. (7) Finger discs barely expanded. (8) Finger fringes present, but weak on FII and III. (9) Metacarpal ridge absent. (10) Finger III not swollen in adult males. (11) Carpal pad absent. (12) Male excrescences on thumb absent. (13) Thenar tubercle present, small. (14) Black arm gland in adult males absent. (15) Tarsal keel thick, long, to mid-tarsus, weakly curved. (16) Toe discs weakly expanded. (17) Toe webbing vestigial between TII and TIII and TIII and TIV. (18) Metatarsal fold absent. (19) External coloration with dark paracloacal marks, thighs with 4–6 transverse narrow bars, usually very conspicuous, sometimes less notorious; with dark brown (not pale) dorsolateral stripes; ventrolateral stripe absent; oblique lateral stripe absent; green chromatophores usually present. (20) Gular-chest markings absent. (21) Dermal collar absent. (22) Male throat coloration immaculate white to white with dark brown spotting; female throat coloration immaculate white to white with few irregular dark brown spots. (23) Male abdomen color white almost immaculate to spotted or reticulated to dark with white spots. (24) Female abdomen color pattern white with few to many small irregular dark brown spotting or reticulum. (25) Iris coloration black with metallic intrusions, gold-colored pupil ring. (26) Large intestine mainly unpigmented. (27) Enlarged white testes. (28) Median lingual process absent. (29) Tympanum inconspicuous, tympanic annulus absent. (30) Vocal sac distinct. (31) Teeth present on the maxillary arch. (32) Size small, males ( n =8) 19.8–24.6 mm , mean=22.0±2.1; females ( n =4) 24.2–25.5 mm , mean=24.8±0.5. Comparisons. Aromobates ornatissimus (characters in parenthesis) differs by its smaller size from larger species of the genus, such as A. alboguttatus (Boulenger, 1903) , A. leopardalis ( Rivero, 1978 ) , A. meridensis (Dole & Durant, 1973) , A. nocturnus ( Myers, Paolillo & Daly, 1991 ) , and A. capurinensis ( Péfaur, 1993 ) , all with maximum SVL> 31.0 mm (up to 25.5 mm ). Aromobates meridensis also lacks dorsolateral stripes and males have a strong reticulation on the belly, but males have preaxial swelling on third finger (absent), and females lack spotting or reticulation on the belly (present). The call is also very different, being a fluttery trill in A. meridensis -Dole & Durant 1973; Barrio-Amorós et al. 2010a- (single notes). Aromobates nocturnus is A. ornatissimus largest congener, and we compare both as they might be sympatric and syntopic; its SVL reaches 62 mm ( 25.5 mm ), it has fully webbed toes (basally webbed), belly coloration uniformly grey to mottled with grayish white in life (dark brown with small to large white spots or white with dark spots or reticulum), and distintive mercaptanlike odor (no apparent odor). Information about myological and osteological characteristics of A. ornatissimus are unknow. The seven other species of Aromobates ( A. duranti ( Péfaur, 1985 ) , A. haydeeae ( Rivero, 1978 ) , A. mayorgai (Rivero, 1980) , A. molinarii ( La Marca, 1985 ) , A. orostoma ( Rivero, 1978 ) , A. saltuensis (Rivero, 1980) , and A. serranus (Péfaur, 1985)) , are similar in size to Aromobates ornatissimus . Aromobates duranti is a slightly larger frog, up to 30.2 mm (up to 25.5 mm ), and has also a dark venter with white spots, but it has clear pale dorsolateral stripes (absent), lateral oblique stripe present but indistinct (absent), skin smooth without tubercles (with tubercles posteriorly), and inhabits a different region of the Cordillera de Merida, separated by 140 km . Aromobates haydeeae has no fringes on fingers (fringes on FII and FIII), tarsal fold ill-defined (well defined), pale dorsolateral stripe present (absent), tympanum distinct inferiorly (inconspicuous), ventral parts orange (dark brown with white spots). Aromobates mayorgai has ventral parts yellow (dark brown with white spots), pale dorsolateral stripe present (absent), and occurs 165 km apart, in a different portion of the Cordillera de Merida, southwestern Sierra de la Culata. Aromobates molinarii has FII and II equal in length (FI longer than FII), fringes absent on fingers (fringes on FII and FIII), tarsal keel ill defined (weakly curved and well defined), toes moderately webbed (basally webbed), pale dorsolateral stripe present (absent), and toe webbing moderate (basal). Aromobates orostoma has no fringes on fingers (fringes on FII and FIII), tympanum distinct inferiorly (inconspicuous), ventral coloration immaculate (dark brown with white spots), pale dorsolateral stripe present (absent), and crossbars on hind limbs are not well defined (welldefined). Aromobates saltuensis has pale dorsolateral stripe present (absent), supratympanic bulge absent (illdefined but present). Aromobates serranus has a distinct tympanum (indistinct), dorsum brown with blotches or reticulum (usually pale brown with dark spots or lines, but not reticulated), pale dorsolateral stripe present (absent), oblique lateral stripe present (absent). Aromobates tokuko from Sierra de Perijá—a separated Andean branch—has pale dorsolateral stripes present (absent), oblique lateral stripe present (absent), and ventral pattern absent (present). We also compare Aromobates ornatissimus with the two only Andean Allobates . Allobates algorei and A. humilis have no webbing on toes (vestigial between TII-III-IV-V), the ventrolateral stripe is present (absent), the oblique lateral stripe is present, diffuse (absent), and are ventrally patternless (striking pattern). Phylogenetic relationships. Aromobates ornatissimus is well nested within Aromobatinae and has a high support as Aromobates ( Fig. 1 ). This species is one of the closest living taxa to the crown of Aromobates and most likely resembles the oldest lineages of Aromobatinae and Dendrobatidae . Therefore, some of the unique and assumed basal characteristics associated to the putative oldest dendrobatid (i.e. Aromobates nocturnus ), such as nocturnality, aquatic lifestyle, and defensive mercaptanlike odor might be derived rather than basal among dendrobatids (as Myers et al. , 1991 ). Individuals of Aromobates ornatissimus were previously characterized using molecular markers at least two times ( Fig. 1 ). Grant et al. (2006) provided nuclear and mitochondrial sequences from an individual collected by Walter Schargel (WES 626). The reported collection locality of this individual is a road from Humocaro Bajo to Agua de Obispo (approximate latitude 9.6945 N and longitude 70.0790 W ) at 2400 m .a.s.l. (Trujillo State, Venezuela ). Vences et al. (2003) provided a short 16S sequence from an individual identified as Nephelobates sp. ULABG 4445. The reported locality of collection is Agua de Obispo (latitude 9.70389 N , longitude 70.1072 W ) at ~ 2200 m .a.s.l. (Trujillo State, Venezuela ). Both localities (i.e., Grant’s and Vences’) and those reported here are within a 10 km radius and, therefore, likely represent a single population of Aromobates ornatissimus . Description of the holotype . The holotype is an adult male of 24.4 mm (SVL): body slender and elongate, rounded in cross-section; dorsal skin, including dorsal surfaces of hind limbs, smooth in preservative ( Fig. 2 A); ventral skin smooth ( Fig. 2 B); vocal sac extended; head is longer than wide, HeL = 34.8% of SVL; HW = 30.3% SVL ( Table 1 ); snout is truncate in profile ( Fig. 3 A), rounded in dorsal and ventral view ( Fig. 3 B); nares are situated laterally to the tip of snout; narial openings are barely visible when viewing the head from the front, barely visible when viewing dorsally; and not seen when viewing from a ventral aspect; canthus rostralis is rounded, the loreal region is little concave; interorbital region is little wider than the upper eyelid; snout longer than ED; tympanum is inconspicuous, about 2/3 of the tympanum is concealed posterodorsally by a low supratympanic bulge formed by the superficial slip of m. depressor mandibulae; tympanum is positioned closely behind eye and lower, close to the angle of jaws; teeth present on maxillary arch; vocal slits large and long, from mid-level of tongue to the angles of jaws; tongue rounded, half free posteriorly. FIGURE 1. Maximum likelihood tree of Aromobates ornatissimus sp. nov. , other dendrobatids, and closely related hyloids. The phylogeny was inferred using a 12S-16S rRNA segment. Support values are based on 200 non-parametric bootstrap replicates. Museum catalog numbers and GenBank accession numbers are provided. TABLE 1. Measurements (in mm) of adult males and females of Aromobates ornatissimus sp. nov. Abbreviations are defined in the materials and methods section. Values are means ± standard deviations; maximum and minimum values are in parentheses. Character Males (n= 8) Females (n= 4) SVL 22.0±2.1 (19.0–24.6) 24.8±0.5 (24.2–25.5) SL 10.2±0.8 (8.8–11.3) 11.4±0.7 (10.5–11.1) FL 10.4±1.0 (8.6–11.8) 11.4±0.5 (11.2–12.1) HeL 8.2±0.5 (7.6–9.0) 8.8±0.2 (8.7–9.0) HW 7.2±0.6 (6.4–8.0) 7.6±0.4 (7.2–8.1) ED 2.7±0.2 (2.3–2.9) n =3; 3.0±0.1 (2.9–3.1) TD n =3; 1.0±0.3 (0.7–1.2) – F3D 0.7±0.1 (0.6–0.8) n =3; 0.9±0.1 (0.8–0.9) T4D 0.8±0.1 (0.7–0.9) n =3; 1.0±0.2 (0.8–1.1) 1FiL 4.0±0.4 (3.1–4.7) n =3; 4.3±0.1 (4.3–4.4) 2FiL 3.6±0.4 (2.8–4.1) n =3; 3.8±0.3 (3.5–4.0) FIGURE 2. Dorsal (A) and ventral (B) views of the holotype (EBRG 5292) of Aromobates ornatissimus sp. nov. Hand ( Fig. 3 C) of moderate size (26.1% SVL); relative lengths of adpressed fingers are III> I> II> IV; discs of all fingers are slightly expanded, horizontally oval; FIII is barely wider than distal end of adjacent phalanx; the base of palm has a large, nearly triangular palmar tubercle; and on base of FI there is a smaller (approximately 1/2 of the palmar tubercle), elliptical thenar tubercle; one or two subarticular tubercles on fingers (one each on FI and FII, two each on FIII and FIV, the distal one of FIV inconspicuous); and all tubercles are flat and round; without supernumerary tubercles. Fringes on fingers are low and indistinct on FII and FIII of right hand (well preserved), and very notable on all fingers of left hand (somewhat dehydrated). FIII is basally swollen on left hand, but not on right hand, possibly also due to a preservation artifact (see Variation). Hind limbs are of moderate length, SL = 43.8% of SVL; relative lengths of adpressed toes are IV> III> V> II> I; TI is moderately long, the tip reaching the mid-subarticular tubercle of TII; toes are slightly expanded, TIV about 1.4 times wider than distal end of adjacent phalanx; feet ( Fig. 3 D) basally webbed; formula only applicable for TII – IV: II 2 – 3 III 3 2/3 – 4 IV; fringes on toes absent; one to three non-protuberant, small subarticular tubercles are present (one on TI and TII, two on TIII and TV, three on TIV, proximal one almost indistinct); two metatarsal tubercles present, including a small round outer, and a similar in size oval inner tarsal tubercle; well defined tarsal keel, thick, weakly curved, transverse across tarsus, from proximal edge of inner metatarsal tubercle to midtarsus; cloacal opening at upper level of thighs, with short tube flap or anal sheath. FIGURE 3. Lateral view of the head (A), dorsal view of the head (B), palmar view of the right hand (C) and plantar view of the right foot (D) of the holotype (EBRG 5292) of Aromobates ornatissimus sp. nov. Scale equals 2 mm. Measurements of holotype (in mm) SVL: 24.4; SL: 10.7; FL: 10.7; HeL: 8.5; HW: 7.4; ETS: 3.9; EN: 1.5; ED: 2.7; TD: 1.2; F3D: 0.7; T4D: 0.8; 1FiL: 4.0; 2FiL: 3.6. Color: In preservative dorsum pale brown with a clear pattern of dark brown marking as follows: interorbital irregular band, one round spot behind the head; two scapular “comma” like symmetrical spots; one longitudinal elongate mark; and two symmetrical longitudinally oval sacral spots. There are two symmetrical dark brown dorsolateral stripes (note that the dorsolateral stripes lacking are the pale ones), from the tip of snout through the canthal ridge, supratympanic area to the groin. Oblique lateral and ventrolateral stripes are absent. The flanks are pale brown below the dorsolateral stripes, with irregular dark brown marks that look similar to a dark oblique stripe, but that are part of an irregular reticulation of the flanks. Below this reticulation, the inferior part of the flanks becomes white. Arms are pale brown dorsally, with one irregular spot (oval on the left forearm, elongate on the right one), with dark brown stripes longitudinal anterior and posteriorly along the arm. Hind limbs pale brown, thighs crossed by many dark brown stripes (six on the left leg, five on the right), shanks crossed by four dark bands on each, and tarsi also crossed by many vertical bands. Ventrally the chin, throat, chest and belly are white with irregular dark brown spotting. Thighs white anteriorly, spotted posteriorly. Palms of the hands and soles of the feet are dark brown. Coloration in life. Description is based on two males, CVULA 8351, EBRG 6146 and one female EBRG 6147 ( Fig. 4 A, C, E). Background dorsal coloration pale to dark brown, with sometimes contrasting dorsal pattern (CVULA 8351, EBRG 6147), or not (EBRG 6146). The pattern, consisting in small spots or lines, is always dark brown. EBRG 6146 has an important portion of the back and flanks covered by dark green chromatophores ( Fig. 4 C). Green chromatophores are also appreciable in EBRG 6147 ( Fig. 4 E), but in less proportion; they are emerald green in EBRG 6147. CVULA 8351 lacks any green chromatophores ( Fig. 4 A). This species seems the only Aromobates to present some amount of green in the body. EBRG 6146 also shows some pale blue chromatophores on the flanks and along the extremities, but less dense than the green chromatophores ( Fig. 4 C). EBRG 6147 has a few pale blue chromatophores on the flanks and shanks ( Fig. 4 E). The dark dorsolateral stripes are present in all three specimens, ill-defined in CVULA 8351, little contrasting in EBRG 6146, or very contrasting in EBRG 6147. A dark brown canthal and supratympanic stripes are present and evident in EBRG 6147 ( Fig. 4 E). All types of pattern on dorsal surfaces (back marks and limbs crossbars) are always dark brown. The three specimens (CVULA 8351 and EBRG 6146-7) have white spots, large or small and irregular on the upper and lower lips. These specimens show axillary and groin marks; on CVULA 8351 and EBRG 6147 are yellow, while on EBRG 6146 are orange. Axillary and groin coloration extends to the ventral surfaces of arms and hind limbs, where it becomes paler. Ventrally, the background color is dark brown on the belly with contrasting white spots, varying from small on CVULA 8351 ( Fig. 4 B), to medium sized in EBRG 6146 ( Fig. 4 D), to large in EBRG 6147 ( Fig. 4 F). The throat color is sexually dimorphic, as the males have a dark brown vocal sac concealed behind the same pattern as on the belly. In the female, throat pattern is contrasting against the belly coloration. Iris coloration is pale to dark copper. Variation. The dorsal pattern (http://images.morphbank.net/?id=705231&imgType=jpeg&sessionId=26djv87rehd8djt 7t 8qbkrgj52) observed in the holotype is similar in 6 specimens ( holotype , EBRG 5282, 5288-91), while others are more spotted (EBRG 5287, 5293), less spotted or almost plain (EBRG 5285, 5294, 5299). Some specimens also have the cross barred limbs, but they are less evident. Ventrally, the pattern is more obvious on five males of the series: the most contrasting individual is EBRG 5291, while the less patterned is EBRG 5294. This last specimen and EBRG 5287 are subadult males and they are almost white without magnification (http://images.morphbank.net/?id=705231&imgType=jpeg&sessionId=26djv87rehd8djt 7t 8qbkrgj52). Under a microscope, both have the throat and chest with melanophores, but the belly is white in EBRG 5294 while it has some reticulation in EBRG 5287. One female (EBRG 5288) is completely white except for a few irregular spots on the belly, and a few melanophores seen with microscope, scattered across the ventral parts. Two females (EBRG 5289 and 5299) have white throats and chests, but their bellies are reticulated (EBRG 5299) or spotted (EBRG 5289). The female EBRG 5298 is darker and both its throat and belly have a profusion of small and irregular spotting. The anal sheath is evident in the holotype and EBRG 5294, but it is not in the males EBRG 5282, 5291, 5293 or barely evident in EBRG 5285, 5287, and 5290. In females, the anal sheath is less evident that in males as evidenced in all specimens (EBRG 5288-89 and 5299). Our observations about the anal sheath agree with Grant et al. (2006) , who suggested that the variation in this character might be caused by preservation artifacts. The holotype has a basal swelling on the left hand FIII, but since there is no other specimen showing this character, we assume this might be an artifact. Specifically, the left hand of the holotype was a little dehydrated and thus, the swelling is due to the preservation. We could not find more sexually dimorphic characters other than the little bigger mean size of females ( 24.8 mm versus 22.0 mm of the males). However, the size range is large and some males are quite big (males up to 24.6 mm , females up to 25.5 mm ). The coloration is mostly not sexually dimorphic with the exception of actively calling males, in which the vocal sac becomes dark. Remarks. Aromobates ornatissimus stands next to the type species A. nocturnus and other two species, A. leopardalis and A. meridensis , in having no pale dorsolateral stripes; Aromobates ornatissimus is the only species with dark dorsolateral stripes (not pale as usual in other Aromobates ), and with presence of green chromatophores (though probably not in all specimens, Fig 4 A). The presence of green chromatophores is remarkable, as it is uncommon among non-aposematic dendrobatids in Venezuela . However, the presence of green chromatophores is also first reported herein for Mannophryne oblitterata and M. sp. aff trinitatis from northern lowlands of National Park Henri Pittier (F. Rojas-Runjaic, pers. com.). Aromobates ornatissimus is also the most ventrally ornamented Aromobates ; this ventral coloration is also evident in three other Venezuelan aromobatines. Two are congenerics from the same Cordillera de Merida, Aromobates duranti and A. meridensis , and on the other hand, Anomaloglossus rufulus from the Chimanta Massif in the Venezuelan Guayana (Barrio-Amorós & Santos, in press ). The aposematic status of Aromobates ornatissimus and these other three species is unknown. Aromobates ornatissimus did not have a distinctive mercaptanlike odor and more likely lack a defensive mechanism required for aposematism. FIGURE 4. Living specimens of Aromobates ornatissimus sp. nov. , showing dorsal and ventral patterns and coloration. Dorsolateral (A) and ventral (B) views of CVULA 8351. Dorsolateral (C) and ventral (D) views of EBRG 6146. Dorsolateral (E) ventral (F) views of EBRG 6147. All specimens photographed are from the type locality. FIGURE 5. Series of 5 notes in the call of Aromobates ornatissimus sp. nov. , taken at 19ºC. (A) waveform and (B) spectrogram. Note distinct harmonics. FIGURE 6. One note of the call of Aromobates ornatissimus sp. nov. taken at 19ºC. (A) waveform and (B) spectrogram. FIGURE 7. Distribution of Aromobates ornatissimus sp. nov. in the Venezuelan Andes. Natural history. Aromobates ornatissimus is active during daytime. The type locality of this species is a creek in an open area (deforested for cattle meadows). The stream also goes through dwarf cloud forest. We found specimens both in the clearings and in the forest. The males were actively calling the 18th of March 2011 , as this day was cloudy, and rain felt during the past weeks. The calling males are never exposed; they call among herbaceous junks, dense aquatic vegetation, or from holes in parts of the cascading stream. In the type locality, we did not found any specimen far from the stream. But in another locality nearby, in a primary cloud forest, the males called from along and away of small streams. We found one male calling from within an artificial abandoned stone-wall inside the cloud forest. No tadpoles were seen despite the fact that we went looking for them in nearby stream pools were the males were calling. Vocalization. Four sets of vocalizations with a total of 35 pulses were recorded at 19ºC air temperature, during the morning of the 18th March 2011 at the type locality. Three sets were from isolated uncollected males with distant chorus on the background and one set from a chorus of at least 3 males . From one the sets representing a single male, we choose five notes of a continuous vocalization lasting a total of 12.65 sec for analysis; Fig. 5 A shows waveform, Fig. 5 B shows spectrogram. We examined the spectrogram of this set and we found a total of 6 to 7 harmonics per note with little frequency modulation as indicated in Fig. 5 B. A single call consists on a single pulsed note emitted every 2.62 to 3.16 sec. The dominant frequency is at 3057-3120 Hz, ( Fig 6 A shows waveform of a single pulse; Fig. 6 B shows the spectrogram). We also measured the note and inter-note duration and from each variable the mean, SD and range in seconds are indicated as follows: note duration: 0.10±0.01 (0.08–0.11); internote duration: 2.86±0.25 (2.62–3.16). Distribution. Aromobates ornatissimus is currently only known from five localities (1) at stream about 3 km before arriving to Las Palmas from Carache, (2) the type locality at Las Palmas, (3) at two streams within a cloud forest on the road from Aguas de Obispo to Barbacoas, (4) along the road from Humocaro Bajo to Agua de Obispo, and (5) at Aguas de Obispo. All of these localities are in the northwestern slopes of Páramo Cendé, Cordillera de Merida, Estado Trujillo ( Fig 7 ). Etymology. ornatissimus (from Latin ornatus ), adjective meaning ornate, elegant or decorated, and –issimus , suffix indicating superlative, then something very ornate, in reference to the striking pattern of the species compared with the rest of congeners, which are less patterned. Conservation. Aromobates ornatissimus is abundant at the type locality and surroundings (about 10 km in circumference). We did not explore exhaustively the type locality for potential conservation threats, but our impression is that Aromobates ornatissimus is surviving and reproducing. In appropriate areas, it was easy to listen from 5 to 20 males calling every 10 m along streams. Due to its reduced and apparently fragmented distributional range, we propose Aromobates ornatissimus as vulnerable VU B2ab(i,ii). A note on Aromobates nocturnus . The general area of Aromobates ornatissimus also includes the type locality of Aromobates nocturnus , and we searched for this iconic species during the day and night in two different streams that resemble those on Figs 13 and 14 in Myers et al. (1991) without success. One of those streams was heavily polluted by cattle manure and the other had apparently suitable habitat, but devoid of any amphibian during the day or night. The current situation of Aromobates nocturnus might be extremely critical, likely extinct, despite it being at the highest category of risk (CR A2a; B2ab5) ( Stuart et al. 2008 ). No concrete plans or actions for the conservation and preservation of the only known population of A. nocturnus exist.