Shewanella marisflavi sp. nov. and Shewanella aquimarina sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea Author Jung-Hoon Yoon Author Soo-Hwan Yeo Author In-Gi Kim Author Tae-Kwang Oh text International Journal of Systematic and Evolutionary Microbiology 2004 54 2347 2352 journal article 10.1099/ijs.0.63198-0 Description of Shewanella marisflavi sp. nov. Shewanella marisflavi (ma.ris.fla 9vi. L. gen. neut. n. maris of the sea; L. masc. adj. flavus yellow; N.L. gen. n. marisflavi of the Yellow Sea). Cells are straight rods, 0·8–1·1×2·0–3·5 µm on MA. Nonspore-forming. Motile by means of a single polar flagellum. Colonies are smooth, glistening, circular, flat to slightly raised, light brown in colour and 2·0–4·0 mm in diameter after 3 days incubation at 30 °C on MA. Growth occurs at 4 and 42 °C, but not above 43 °C. Growth is observed at pH 5·0, but not at pH 4·5. Optimal growth occurs in the presence of 2–3 % NaCl. No growth occurs in the presence of more than 9 % NaCl. Growth occurs under anaerobic conditions on MA. Casein, tyrosine and Tween 80 are hydrolysed. Aesculin, hypoxanthine, urea, xanthine and xylan (birch wool) are not hydrolysed. When assayed with the API ZYM system, alkaline phosphatase, esterase ( C 4), esterase lipase ( C 8), α-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N -acetyl-βglucosaminidase are present and leucine arylamidase is weakly present, but lipase ( C 14), valine arylamidase, cystine arylamidase, trypsin, α-galactosidase, β-galactosidase, βglucuronidase, α-glucosidase, β-glucosidase, α-mannosidase and α-fucosidase are absent. Acid is produced from D-cellobiose, D-glucose, maltose and D-ribose. Acid is not produced from L-arabinose, D-fructose, D-galactose, lactose, D-mannose, D-melezitose, melibiose, D-raffinose, L-rhamnose, stachyose, sucrose, D-trehalose, D-xylose, adonitol, D-mannitol, myo -inositol or D-sorbitol. Both menaquinones and ubiquinones are present; the predominant menaquinone is MK-7 and the predominant ubiquinones are Q-7 and Q-8. The major fatty acid is iso-C15: 0. The DNA G+C content is 51 mol% (determined by HPLC ). Other phenotypic characteristics are given in Table 1 . The type strain ( SW-117 T = KCCM 41822 T = JCM 12192 T ) was isolated from sea water of the Yellow Sea in Korea .