A new species of the genus Empitrombium Southcott, 1994 (Acari: Microtrombidiidae) from Turkey Author Sevsay, Sevgi Author Karakurt, İbrahim text Zootaxa 2013 3709 3 255 266 journal article 10.11646/zootaxa.3709.3.4 d65b8fed-cbbf-40de-ad9c-2a8e788fd50f 1175-5326 217073 CEB8533C-E3F5-45E3-A119-D87C2A9BD39F Empitrombium makolae sp. nov. Diagnosis. Adult. Body length 1300–1900, width 850–1400. Distal ctenidium of palp tibia made of 5–6 spinisetae (plus paradont), proximal ctenidium composed of 8–12 spinisetae, radula consists of 6–13 spine-like setae; lateral face of palp tibia with one long and strong basidont; crista metopica with rounded sensillary area, anterior and posterior processes; long accessory posterior process present; double eyes almost sessile, anterior lenses distinctly larger than posterior ones; dorsal opisthosomal setae ( pDS ) uniform (15–20) with wide, cylindrical or slightly fusiform stem, three times longer than wide, all covered with very delicate setules; all setae based on distinct papillae with regular rim; legs without lamellar processes; tarsus I elongate, at least 1.5 as long as wide. Deutonymph. Generally smaller (body length 950–1080 width 590–650). Medial face of palp tibia much simplified, with only distal ctenidium made of 4–5 spinisetae (plus paradont) and radula with 2–5 spine-like setae; lateral face of palp tibia with one basidont; dorsal opisthosomal setae similar to those in adults. Larva. Gnathosoma with horseshoe-like sclerite bearing large denticled membranes outside. Palp femur and genu bearing one spine-like seta each and palp tibia with three nude setae. Setae bs in shape of stout calyx, distally seven finger-like extensions. Surface of scutum bears three pairs of nonsensillary setae and one pair of sensillary setae. Smooth up to AM bases and whole remaining part striate and punctate. ƒCx formula: BB-B-B. Tarsal claws formula: 2-2-3. Ta III terminated with two falciform claws and a slender claw-like empodium. Lophotrix and scopa absent on tarsus III. Descriptions. Holotype . Female Standard measurements in Table 1 . Body is red colour in life. Idiosoma broadened at shoulders, narrowing posteriad ( Fig. 1 ). Body length 1805, width 1285. TABLE 1. Morphometric data for postlarval forms of Empitrombium makolae sp. nov .

Character	Holotype	Female (n=4) min – max	Male (n=8) min – max	Deutonymph (n=5) min – max
LB	1805	1315–2100	1300–1880	1007–1300
WB LB/WB Ch BS (L) Ch BS (W)	1285 1,4 223 95	934–1350 1.4–1.5 177–222 73–90.	850–1195 1.5 115–183 70–87	665–920 1.4–1.6 112–145 50–58
Ch Cl PaTr (L)	73 117	58.2–76 95–124	40–62 59–67	36–56 32–42
PaTr (W) PaFe (L) PaFe (W)	95 244 179	91–97 170–253 165–189	61–66 102–178 111–127	48–59 100–118 81–103

. .....continued on the next page . .....continued on the next page Gnathosoma. Palps strong ( Fig. 2 ). Medial face of palp tibia with two, often overlapping ctenidia and radula. Distal ctenidium composed of stronger setae than proximal one. Distal ctenidium made of 5–6 (plus paradont) spinisetae situated behind paradont; proximal ctenidium composed of 8–12 uniform spinisetae; radula consist of 13-13 long and very strong spine-like setae ( Fig. 3 ). Lateral face of palp tibia ( Fig. 4 ) with one long (60–75) and strong basidont placed at base of palp tarsus and with one long, smooth, whip-like setae at base of odontus. Palp tarsus characteristic, long and cylindrical with numerous feather-like setae and 2–3 solenidia in distal part. TABLE 1. (Continued)

Character	Holotype	Female (n=4) min – max	Male (n=8) min – max	Deutonymph (n=5) min – max
PaGe (L)	131	118–140	71–105	46–53
PaGe (W) PaTi (L)	109 141	81–109 114–148	69–76 81–103	47–69 47–67
PaTi (W) Odo (L) (Lf/Rt) Par (L) (Lf/Rt) diCt(n) (Lf/Rt)	63,2 73/75 32/36 4+par/5+par	46–65 65/85 24/26 4–5+par/4–5+par	32–50.2 53–69/69–73 18–29/27–33 4–5+par/3–5+par	22–41 44–49/46–52 17–39/23–41 4+par/4–5+par
prCt(n) (Lf/Rt) Bas (n)(Lf/Rt)	12/7 1/1	8–12/7–10 1/1	4–6/5–6 1/1	1/1
Bas (Lf/Rt) (pr ⊣ di) Rad (n)(Lf/Rt)	55 13/13	57/60 13–16/12–13	41–54 6–9/7–12	35–350 3–5/2–3
PaTaSol(n) PaTa (L) PaTa (W)	3–4 103 31	2–4 92–112 23–35	2–3 65–93 21–30	2–3 32–42 11–13
mdS [S] mdS [P] pdS [S]	13–18 2.5–3.5 15–20	13–16 2.2–3.6 15–20	10–18 2.2–3.4 12–17	22–25 2.5–4 13–20
pdS [P] vS [S] vS [P]	2.3–3.4 23–35 2.2–3.7	2.2–4.1 20–46 2.2–3.8	2.4–3.6 20–40 2.1–4.1	3–5.4.9 25–46 2.5–3.2
CML CMW ASB	367 32 43	295–405 25–39.4 29–48	280–334 16–29 35–54	193–246 14–23 30–32
PSB AM (n) AM (L)	20 32 50–85	17–23 19–35 40–83	18–22 22–28 45–58	19–21 9–15 38–48
RCM SAL SAW	227 62 67	176–218 57–68 49–66	121–159 52–66 48–64	99–130 28–57 43–52
SB SE pPr	46 151 60	24–50 100–165 55–90	22–36 95–145 85–105	24–25 70–130 48–60
acpPr OL OCM	33 64 118	24–42 58–65 92.5–142	20–23 49–64 79–113	8–14 41–68 77–117
aO pO O-O	21 18,9 264	20.8–24.2 18.2–18.9 201–266	16–27 11–21 123–251	15–19 9–18 147–249
OaD OSD GOp	138 86.2 198	132–155 83–89.2 195–199	94–117 63–95 165–185	57–91 65–67 83–107

TABLE 1. (Continued)

Character	Holotype	Female (n=4) min – max	Male (n=8) min – max	Deutonymph (n=5) min – max
gs [S]	35	32–35	18–31	15–26
pgs [S] An (L)	28 92	28–42 73–89	19–34 110–140	24–41 40–51
An La Cx_I Tr_I Bf_I	15 219 140 254	13–16 188–224 90–146 141–246	17–26 135–167 83–104 120–152	21–24 95–109 55–78 57–81
Tf_I Ge_I	178 202	135–201 164–211	102–143 98–176	60–89 80–102
Ti_I Ta_I (L) Ta _I (W) Ta _I (L/W)	205 283 187 1,5	170–187 237–296 155–190 1.5	115–172 198–239 120–143 1.6	90–117 138–170 96–112 1.4–1.5
Leg I Cx_II	1668 205	1125–1511 186–223	851–1153 140–188	575–746 100–110
Tr_II Bf_II Tf_II Ge_II	109 123 107 127	90–128 70–131 91–113 109–139	68–79 70–91 70–74 80–95	42–57 49–55 45–56 52–63
Ti_II Ta_II	154 225	120–179 164–285	98–114 141–189	62–71 91–126
Leg II Cx_III Tr_III Bf_III	1050 225 160 110	830–1198 154–241 148–192 97–123	667–830 120–149 78–86 80–90	441–538 83–102 41–56 32–52
Tf_III Ge_III	140 159	93–166 109–181	62–71 72–97	39–51 48–62
Ti_III Ta_III Leg III Cx_IV	234 281 1309 226	112–229 170–291 883–1423 232–261	92–107 125–143 629–743 165–177	60–82 84–116 387–521 102–117
Tr_IV Bf_IV	105 121	102–147 118–148	88–93 96–106	49–72 53–66
Tf_IV Ge_IV Ti_IV Ta_IV	110 127 128 219	106–136 113–163 124–197 212–232	100–144 112–155 136–149 150–198	62–67 77–88 89–95 98–122
Leg IV IP	1036 5063	1007–1284 3845–5416	847–1022 2994–3748	530–627 1933–2432

FIGURES 1 – 8. Empitrombium makolae sp. nov. , female (holotype). 1. general view, in transparency, setae omitted. 2. Palp, medial aspect, setae omitted. 3. Palp tibia and tarsus, medial aspect. 4. Palp tibia and tarsus, lateral aspect. 5. Crista metopica region. 6. Dorsal opisthosomal setae (pDS). 7. Genital opening. 8. Genu, tibia and tarsus I, setae omitted. FIGURES 9 – 15. Empitrombium makolae sp. nov. , male and deutonymph. 9. Male, crista metopica region. (10–15) Deutonymph. 10. Crista metopica region. 11. Palp tibia and tarsus, medial aspect. 12. Palp tibia and tarsus, lateral aspect. 13. Dorsal opisthosomal setae. 14. Genital opening. 15 . Leg I, omitted setae. Idiosoma. Aspidosoma triangular in outline. Anterior part of crista metopica relatively broad, almost not fused with weakly sclerotized vertex which bears several long, setulose, nonsensillary setae ( AM ) ( Fig. 5 ); rounded sensillary area bears two short, smooth sensillary setae; posterior process relatively short and indistinct; accessory posterior process present and clearly prolonged. Double and sessile eyes, located at half length of anterior part of crista metopica; anterior lenses distinctly larger than posterior lenses. Dorsal opisthosomal setae ( Fig. 6 ) uniform (15–20), more than three times longer than wide, with broad, cylindrical stem, all covered with very delicate long setules; mid-dorsal seta of shorter than post-dorsal ones (pDS); all setae based on with regular rim. Ventral seta slightly longer (18–29). External genitalia composed of a pair of epivalves and centrovalves covered densely with setae. Epivalves shorter than centrovalves ( Fig. 7 ) with three pairs of genital acetabula. Legs. Legs without lamellar processes, shorter than idiosoma. Tarsus I elongate (210–280 long, 130–190 wide), tibia I shorter than tarsus I ( Fig. 8 ). Male: Generally similar to female; body shorter than female, body length 1300–1600, width 850–1100. Posterior part of crista metopica longer than female and wide vertex strongly sclerotized ( Fig. 9 ). Male anus (110– 120) much bigger than in female (85–95). Deutonymph: Standard measurements in Table 1 . Similar to adult but smaller. Anterior part of crista metopica not fused with vertex which bears a few long, setulose, nonsensillary setae ( AM ) ( Fig. 10 ). Palps similar to those in adult but smaller and not as robust as in adult. Medial face of palp tibia ( Fig. 11 ) much simplified, with only one ctenidium composed of 4–5 spinisetae (excluding paradont) and radula with 2–5 spine-like setae. Lateral face of palp tibia ( Fig. 12 ) with one basidont. Palp tarsus with numerous feather-like setae. Dorsal opisthosomal setae similar to those in adults but shorter (13–20) ( Fig. 13 ). Two pairs of genital acetabula ( Fig. 14 ). Tarsus I of same proportions as in adults; tarsus I 138–170 long, 96–112 wide ( Fig. 15 ). Larvae: Standard measurements in Table 2 . All larvae reared from eggs deposited by females in the laboratory. Colour in life red. TABLE 2. Morphometric data on larvae of E. makolae sp. nov. and E. pesici . E. makolae E. pesici (n=9) ......continued on the next page Gnathosoma ( Fig. 16 ). Movable gnathosoma with anterior ring-like sclerite (stephanostome) bearing about 20–25 teeth. Setae bs in shape of stout calyx, distally seven finger-like extensions. Cheliceral blade short and slightly curved. Lateral of ring sclerite with one pair of adoral setae. Palpal femur and genu with one small spinelike seta each. Tibia with three nude setae: one long, one very short, one spine-like. Palpal tibial claw bifid. Palpal tarsus with one solenidion, one eupathidium, two very long and three very short nude setae. f Pp formula:0-N-N- NNN2-NNNNNωζ

Character	Holotype	N	Min.–max.	Min.–max.
L	336	10	323–387	240–272
W	182	10	185–230	124–158
L/W	1.84	10	1.46–1.89	1.67–2.52
AA	40	10	37–44	37–50
AW	83	10	84–90	49–81
PW	84	10	86–100	72–84
SB	54	10	53–59	46–52
ASB	99.2	10	85–102	84–94
PSB	29	10	26–32	26–35
AP	37	10	33–38	32–40
AM	43	8	43–54	37–50
AL	55	10	52–59.3	52–62
PL	52	10	51–58	50–62
S	65	3	62–69	64–67
MA	57	10	48–61	50–59
HS	45	10	43–62	43–52
LSS	95	10	94–113	60–91
DS MİN.	52	10	44–58	37–70

TABLE 2. (Continued)
E. makolae	E. pesici (n=9)
Character Holotype	N	Min.–max.	Min.–max.
DS MAX. 60	10	65–76	61–80
or 9	10	8–13	10–15
bs 10	10	9–11	8–12
1a 57	7	52–71	53–62
1b 46	8	48–57	42–58
2b 48	8	46–57	51–62
3a 25	8	20–29	18–23
3b 56	8	48–59	55–59
h1 40	10	43–52	–
h2 38	10	36–53	–
CX_I 63	10	61–79	62–72
TR_I 34	10	29–48	35–50
FE_I 48	10	34–55	50–56
GE_I 29	10	24–28	30–35
Tİ_I 44	10	41–46	42–52
TA_I 104	10	94–104	90–99
LEG I 322	10	302–334	325–348
CX_II 64	10	60–80	57–64
TR_II 35	10	32–38	32–42
FE_II 46	10	40–53	50–54
GE_II 24	9	21–27	25–27
Tİ_II 43	9	41–46	42–52
TA_II 94	9	82–95	79–84
LEG II 306	9	281–328	279–307
CX_III 67	10	61–68	59–64
TR_III 35	10	32–42	40–47
FE_III 55	10	48–59	52–60
GE_III 27	10	23–30	25–32
Tİ_III 49	10	40–52	48–54
TA_III 95	10	89–96	80–89
LEG III 328	10	307–334	313–335
IP 956	9	903–979	937–990

Idiosoma dorsum ( Fig. 17 ). Scutum pentagonal; lateral borders slightly concave near S bases, slightly convex at posterior border and bent anterolaterally (stolascutum). Smooth up to AM bases and whole remaining part striate and punctate. The surface of scutum bears three pairs nonsensillary setae and one pair sensillary setae. AM seta smooth, AL and PL setae similar to each other and barbed. S seta filiform and with two barbs at middle. Two pairs of eyes that include double lens, situated at level between S and PL setae; surface of eye plates, between eye lenses, striate. Anterior lens larger than posterior one. Scutellum triangular and slightly convex at mid-anterior border, with striations and punctations similar to that on scutum, bears one pair of barbed c1 setae. All dorsal setae situated on plates or platelets (the largest c2 and d1 plates) barbed and arranged in five rows. f D formula: 6-6-6-6-4 ( c1-c3 , d1- 3 , e1-3 , f1-3 , h1-2 ). Idiosoma ventrum ( Fig. 18 ). Claparéde’s organs laterally between coxae I and coxae II. ƒCx formula: BB-B-B. Coxa I with barbed 1a and 1b setae with setules. Coxa II and coxa III respectively with seta 2b and seta 3b with setules. One pair of nude intercoxal setae 3a placed in between coxal plates III. Posteriorly following four barbed setae situated on plates anterior and lateral to anal opening. ƒV formula: 2u-2. Ventral setae slightly thinner than dorsal setae. Anal opening without sclerite. Legs. ( Figs. 19–24 )). Legs segmentation formula: 6-6-6. Legs setal formula: [I] Tr (1B) – Fe (5B, 1N) – Ge (4B, 2σ, 1κ) – Ti (6B, 2φ, 1κ) – Ta (17B, 2ζ, 1ω, 1ε); [II] Tr (1B) – Fe (4B, 1N) – Ge (2B, 1σ) – Ti (5B, 2φ) – Ta (13 B, 1ω, 1ε,1 ζ); [III] Tr (1B) – Fe (3B, 1N) – Ge (2B, 1σ) – Ti (5B) – Ta (12B). Tarsus I and tarsus II terminated with two falciform claws; Ta III terminated two falciform claws and a slender claw-like empodium. FIGURES 16 – 18. Empitrombium makolae sp. nov . larvae. 16 . Gnathosoma. 17 . İdiosoma (dorsum). 18. İdiosoma (ventrum). FIGURES 19 – 24. Empitrombium makolae sp. nov. larvae. 19. Leg I (trochanter-genu). 20. Leg I (tibia and tarsus). 21. Leg II (trochanter-genu). 22. Leg II (tibia and tarsus). 23. Leg III (trochanter-genu). 24. Leg III (tibia and tarsus). Etymology. This species is named in honor of Prof. Dr. Joanna Mąkol (Department of Invertebrate Systematics and Ecology, Wroclaw University, Poland ) for her great and valuable contributions to Acarology. Type material. Holotype female collected in Köse mountain, Gümüşhane Province, Turkey (40°14'921 N, 38°39'846 E, 1700 m a.s.l., 0 9.05.2012, leg. H.H. Özbek). Paratypes : same data as for holotype . Larvae obtained from holotype females by experimental rearing. The type material and other specimens were deposited in Acarology Laboratory of Erzincan University, Erzincan, Turkey . Distribution. Turkey . Biology. Oviposition took place under laboratory conditions, at the beginning of May, nine days after collecting the females in the field. The eggs were laid in clutches. The prelarvae appeared 12 days after oviposition, and the larvae after the next 18 days. Altogether, two females oviposited and 10– 16 larvae were obtained from each. The total time between oviposition and emergence of larvae was 22 days.