Reclassification of Cybistrinae Sharp, 1880 in the Neotropical Region (Coleoptera, Adephaga, Dytiscidae), with description of new taxa Author Miller, Kelly B. https://orcid.org/0009-0004-1093-4066 Department of Biology and Museum of Southwestern Biology, University of New Mexico, Albuquerque, NM 87131 - 0001, USA kbmiller@unm.edu Author Michat, Mariano C. https://orcid.org/0000-0002-1962-7976 Instituto de Biodiversidad y Biologia Experimental y Aplicada, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina Author Ferreira Jr, Nelson https://orcid.org/0000-0002-5932-7695 Laboratorio de Entomologia, Departamento de Zoologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasi text ZooKeys 2024 2024-01-08 1188 125 168 http://dx.doi.org/10.3897/zookeys.1188.110081 journal article http://dx.doi.org/10.3897/zookeys.1188.110081 1313-2970-1188-125 997ADB92AFA7497982A2B81C00EF3AEA FD00287A8052513C815877D5359D01A1 Cybister (Neocybister) puncticollis ( Brulle , 1837) Figs 10 , 17 , 18 , 33-35 , 52 , 59 Dyticus puncticollis Brulle , 1837: 46. Cybister puncticollis : Aube 1838: 52. Trogus puncticollis : Gemminger and Harold 1868: 460. Megadytes puncticollis : Sharp 1882 : 709; van den Branden 1885: 29; Regimbart 1889: 267; 1904: 225; Chatanay 1910: 434; Bruch 1915: 479; Zimmermann 1919: 235; 1920: 256; Wilke 1921: 24; Bruch 1927: 543; Blackwelder 1944 : 80; Brinck 1945 : 8; Mouchamps 1957 : 283; Tremouilles and Bachmann 1980 : 105. Cybister (Meganectes) kemneri Brinck, 1945: 18; Tremouilles and Bachmann 1980 : 105. Cybister (Meganectes) puncticollis : Tremouilles and Bachmann 1980 : 105. Cybister (Neocybister) puncticollis : Miller et al. 2007 : 54; Nilsson and Hajek 2023 : 84. Type locality. Cybister puncticollis : Bolivia, San Miguel. Cybister kemneri : Brazil, La Plata, Amazonas, Rio Autaz. Type specimen. Cybister puncticollis , in MNHN (not examined). Cybister kemneri , in MZLU (not examined). Diagnosis. This species is larger (Fig. 10 ) than the other Neotropical species in the genus, C. festae (Fig. 9 ) and the male genitalia are distinctly different (Figs 33-35 ). See above under that species for details about this and additional diagnostic differences between these two species of Cybister . Description. Measurements . TL = 26.6-27.6 mm, GW = 15.0-15.9 mm, PW = 10.8-11.6 mm, HW = 6.5-6.8 mm, EW = 3.7-3.8 mm, TL/GW = 1.7-1.8, HW/EW = 1.7-1.8, WC/WV = 2.9-3.2. Body shape oval, widest slightly posterad of middle; lateral margins broadly curved, continuously curved between pronotum and elytron. Depressed and somewhat flattened in lateral aspect. Coloration . Head dark green, clypeus and labrum pale yellow. Pronotum dark green, with broad lateral yellow marginal band (Fig. 10 ), medial margin of band distinctly delimited, band separated from posterolateral margin by narrow green strip (Fig. 10 ). Elytron dark green with broad lateral yellow marginal band, medial margin of band distinctly delimited, lateral margin of band slightly remote from lateral elytral margin for medial portion of length, extending laterally to margin of elytron anteriorly and posteriorly, apex of yellow band diffusing into coloration of elytron, less distinct (Fig. 10 ). Ventral surfaces mostly black except antennae and palpi orange, pro- and mesothoracic legs mostly orange, tarsi dark orange, metathoracic legs testaceous, anterodorsal surface of tibia orange, propleuron and elytral epipleuron pale orange to testaceous, and with small orange maculae laterally on abdominal ventrites IV-VI. Sculpture and structure . Head broad, anteriorly produced, eyes prominent; dorsal surface evenly covered with exceptionally fine micropunctures but smooth and shiny. Pronotum with lateral margins evenly and shallowly curved; surface of pronotum similar to surface of head in micropunctation; pronotum dorsally evenly curved. Elytron with margins very broadly curved, more strongly curved posteriorly; surface similar to surface of head in micropunctation and surface appearance. Prosternal process anteriorly distinctly emarginate medially, remaining surface nearly flat throughout and moderately broad, apex broadly elongate and sharply pointed. Metaventral wing narrow (WC/WV = 2.9-3.2); surface smooth, with extremely fine microsculpture, shiny. Lateral portion of metacoxa large, broad, surface smooth and shiny, with extremely fine microsculpture; metacoxal lines short and curved, extending anteriorly less than half distance across metacoxa. Abdominal ventrites smooth, relatively unsculptured. Male genitalia . Male median lobe in lateral aspect moderately slender throughout, evenly curved, apically abruptly expanded with a narrowly rounded apex, ventral sclerite slender and evenly curved basally, apically straight and apically pointed (Fig. 33 ); in dorsal aspect median lobe moderately broad basally, lateral margins broadly sinuate, apex broadly expanded, with apex broadly sub-truncate, medially broadly pointed (Fig. 34 ). Lateral lobe moderately broad throughout length, evenly curved, apex narrowly rounded, with long series of setae along dorsal margin (Fig. 35 ). Female genitalia . Vagina extremely elongate, slender; with enlarged area at base of common oviduct and spermatheca, with enlarged lateral sacs on this enlarged area; spermatheca extremely slender and elongate (Fig. 59 ); gonocoxae together slender and apically pointed (Fig. 59 ); gonocoxosternite broad, anterolateral lobe broad, medial margin distinctly, narrowly emarginate, basomedially with field of setae (Fig. 59 ). Sexual dimorphism . Males have a broad protarsal palette with ventral adhesive setae with apical structures in the form of elongate flattened spatulate surfaces; males also have mesotarsomeres with posteroventral fields of setae which are absent in females. Females lack natatory setae along the ventral margins of the metatarsomeres, but these are present in males. Males have a single metatarsal claw (the anterior claw, Fig. 17 ), whereas females have a second, posterior claw that is ~ 1/2 the length of the anterior, is abruptly curved apically and sharply pointed (Fig. 18 ). Variation . Specimens vary somewhat in size and coloration, with some specimens more greenish and others darker, less greenish. Distribution. This species is known from Argentina, Bolivia, Brazil, French Guyana, and Peru. There is also a previously unpublished record from Paraguay. Material examined. Specimens were examined from Argentina , Bolivia , Peru , and Paraguay .