Two new Oriental species of Paramanota Tuomikoski (Diptera: Mycetophilidae), with DNA sequence data Author Hippa, Heikki Author Kaspřák, David Author Kahar, Siti Rafhiah Haji Abd Author Ševčík, Jan text Raffles Bulletin of Zoology 2016 2016-12-07 64 360 367 journal article 10.5281/zenodo.5355716 2345-7600 5355716 20D2CA85-5EC3-4145-8599-9AEEA565F07A Paramanota trilobata , new species ( Figs. 2A–D ) Type material. Holotype . Male ( T 4371 in QSBG ). THAILAND , Petchaburi , Kaeng Krachan NP , Panernthung [km 27 water pump], 12°49.151’N 99°22.483’E , 970 m , Malaise trap , coll. Sirichai & Chusak , 12–19 September 2008 . Female. Unknown. Etymology. The name is Latin, trilobata , three-lobed, referring to the three-lobed gonostylus. Distribution. Thailand . Description. Male. Colour . Head yellowish brown, vertex and antennal flagellum darker brown; setae and other vestiture dark. Thorax yellowish, scutum, scutellum and the medial part of mediotergite brown, anterior part of anepisternum infuscated; thoracic setae mostly worn out, the few existing ones dark, the short setae anteriorly on scutum seem pale. Legs yellowish, hind femur with an infuscated subapical patch ventrally, the setae and other vestiture dark which makes the apical part of tibiae and all of tarsi seem dark under low magnification. Wing yellowish brownish; haltere yellowish with dark brown knob. Abdomen brown, tergites and tergites concolorous, setae dark. Head. Similar to fig 7a in Hippa et al. (2005) but the number of facial setae higher. Antennal flagellomere 4, Fig. 2D . Maxillary palp similar to fig 7a in Hippa et al. (2005) , ultimate palpomere 1.9 times longer than the penultimate one. The strong postocular setae are not countable in the specimen. Thorax similar to Fig. 8a in Hippa et al. (2005) . Legs . Front tibial organ not well visible in the specimen, only one seta observable. Wing similar to fig. 1D in Hippa (2010). Wing length 2.6 (3.0) mm. Hypopygium , Figs 2A–C : Gonocoxae ventrally separated by a membranous area, each side with a very large lobe which is posteriorly extending as far as the gonostylus, with the posterior part simple, not divided into sub-lobes; the ventral surface of the lobe as well as the other ventral parts of gonocoxa evenly covered with strong setae, the dorsal surface of the lobe with a postero-mesial area of numerous megasetae enlarging in size towards the base, a few rather unmodified setae flanking on the lateral side the area with megaseta. Gonocoxa dorsally simple with the setae similar to those of the ventral side. Tergite 9 simple, with strong setae. Tergite 10 fused with tergite 9 and appearing as a small setose lobe at the postero-lateral corner of the latter. Cercus simple. The hypoproct with one seta. Gonostylus with a dorsal lobe, a ventral lobe and a median lobe, each with difficultly observable largely membranous sub-lobes; the dorsal lobe in dorsal view elongated, nearly parallel sided, with membranous apical part showing a striated or lamellar structure; the ventral lobe narrow, oblique, at anterior margin with a long comb-like row of narrow lamellae, the median lobe narrow, oblique, without comb-like structures. Aedeagus elongate, with a broader basal part and narrower almost parallel sided apical part, the details not discernible in the mount. Fig. 2. Paramanota trilobata , new species (holotype). A, Hypopygium, dorsal view; B, Hypopygium, ventral view; C, Outlines of aedeagus and associated structures, dorsal view; D, Antennal flagellomere 4, lateral view. Scale bar = 0.1 mm. cr = cercus, gs d = dorsal lobe of gonostylus, gs m = median lobe of gonostylus, gs v = ventral lobe of gonostylus, gx = gonocoxa, gx l = ventral gonocoxal lobe, hp = hypoproct, tg 9 = tergite 9, tg 10 = tergite 10. Fig. 3. Paramanota bifalx Hippa, 2010 (Thailand) . A, Right half of hypopygium, dorsal view; B, Median lobes and the ventral lobe of gonostylus, dorsal view. Scale bar = 0.1 mm. cm = comb-like row of pale lamellae, cr = cercus, gs d = dorsal lobe of gonostylus, f l = curved finger-like lobe, gs v = ventral lobe of gonostylus, gx = gonocoxa, gx l = ventral gonocoxal lobe, h l = hand-like lobe, hp = hypoproct, tg 9 = tergite 9, tg 10 = tergite 10. Fig. 4. Molecular affiliation of Paramanota rodzayi based on Maximum likelihood analysis of the combined dataset (12S, 28S, and COI). The numbers below the branches indicate bootstrap support (BS) values above 75. Discussion. In the key to the species of Paramanota (Hippa, 2010) , P. trilobata fits couplet 1 including only P. orientalis because the ventral gonocoxal lobe is posteriorly simple, not divided into a more lateral and a more mesial sub-lobes. In this respect P. trilobata is similar to P. rodzayi , the other new species described in the present paper. Both these species are distinguished from P. orientalis by having all the megasetae on the dorsal side of the ventral gonocoxal lobe short, the longest ones at most one fourth the width of the lobe while in P. orientalis they are double of that length. Paramanota trilobata is distinguished from P. rodzayi by having the antennal flagellomeres longer, the flagellomere 4 being as long as broad instead of only half of that length, by having the dorsal aspect of the dorsal gonostylar lobe elongate subquadrangular, not subtriangular, and by lacking a transverse comb-like row of lamellae apically on the median lobe of the gonostylus. Also many other details in the hypopygium distinguish the two species ( Figs. 1 , 2 ).