Two new Hypomyces associated with boletoid fungi in China Author Gu, Xin 0000-0002-0846-1069 School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, P. R. China & guxin 929 @ 163. com; https: // orcid. org / 0000 - 0002 - 0846 - 1069 Author Fu, Rong 0000-0002-6475-355X State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing 100101, P. R. China & 2505617830 @ qq. com; https: // orcid. org / 0000 - 0002 - 6475 - 355 X 2505617830@qq.com Author Wang, Rui 0000-0003-4745-3181 School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, P. R. China & amwangrui @ 126. com; https: // orcid. org / 0000 - 0003 - 4745 - 3181 Author Sun, Jing-Zu 0000-0003-1893-1869 State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing 100101, P. R. China & sunjz @ im. ac. cn; https: // orcid. org / 0000 - 0003 - 1893 - 1869 sunjz@im.ac.cn text Phytotaxa 2021 2021-08-20 516 1 28 42 http://dx.doi.org/10.11646/phytotaxa.516.1.2 journal article 10.11646/phytotaxa.516.1.2 1179-3163 5316520 Hypomyces ampullaris Jing Z. Sun , sp. nov. FIGURE 2 Index Fungorum number: IF558005 Etymology : — The specific epithet refers to its ampullar conidia. Type :— CHINA , Sichuan Province , Yancheng city ( 27°39′31″N , 101°14′16″E ), from a fruiting body of Boletus sp. 8 August 2018 ; Fu Rong & Liu Xing-Zhong ( HMAS 291301 , holotype ), ex - type living culture CGMCC 3.20157 . Fungicolous , growing on a fruiting body of Boletaceae sp. Asexual morph : Hyphomycetous. Mycelium forming a loosely attached layer on the fruiting body of the host ( FIGURE 2a ). On PDA medium, colony forming a thick mat, white at the beginning, then becoming yellow ( FIGURE 2b, c ). Mycelium superficial, copious, developing as floccose masses of septate, branched, warted and hyaline hyphae bearing robust conidiophores with white spore masses ( FIGURE 2b ). Conidiophores hyaline, erect, flexuous, branched, septate, warted. With two types of conidiogenous cells phialidic and aleuriogenic ( FIGURE 2d– f, i ). Type A: Phialides in 2–3 verticels, hyaline, warted, 16–40 × 4–5.5 μm (x̅ = 25 × 5 μm, n = 30), attenuating to 1.5–3.0 μm at their apices ( FIGURE 2d, g, h ). Phialoconidia acrogenous, hyaline, ampulliform, aseptate, 12–18 × 5.5–7.5 μm (x̅ = 14.5 × 6.5 μm, n = 50) ( FIGURE 2k–m ). Type B: Aleuriogenic discrete, on short side branches, hyaline, warted ( FIGURE 2e, f, i ). Aleurioconidia hyaline at the beginning, becoming golden yellow when mature, subglobose to globose, thick - walled, aseptate, with angular tubercles on surface, 20–27 × 19–26 μm (x̅ = 23 × 22.5 μm, n = 50) ( FIGURE 2e, f, i, j ). Tubercles , cylindrical, 1–1.5 μm, often with apical depression, densely arranged ( FIGURE 2j ). Sexual morph: Undetermined . Other material examined :— CHINA , Sichuan Province , Yancheng city ( 27°39′31″N , 101°14′38″E ), from a fruiting body of Boletales sp. 8 Aug 2018 ; Fu Rong ( YY28-1 ), living culture CGMCC 3.20158 . FIGURE 2. Hypomyces ampullaris (CGMCC 3.20157). a Yellow colony on a fruiting body of Boletus sp. b, c Forward and reverse of a colony on PDA. d–g Conidiophores. h Phialides. i Aleurioconidia on short side branches. j Aleurioconidia. k–m Phialoconidia. Scale bars: d–g = 50 μm, h–i = 25 μm, j–m = 10 μm. Notes :—The new species has grown on fruiting bodies of Boletaceae sp. containing only the asexual state. Among the known Boleticolous species of Hypomyces , H . ampullaris is morphologically similar to Sepedonium ampullosporum Damon in forming candelabrum like conidiophores and producing ampulliform conidia. But S . ampullosporum differs in having longer phialides (30–80 × 3.5 μm) and larger ampulloconidia (14–20 × 6–8 μm), and pigment discoloration of medium ( Damon 1952 , Sahr et al. 1999 , TABLE 2 ).