Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding Author Kirichenko, Natalia Author Triberti, Paolo Author Akulov, Evgeniy Author Ponomarenko, Margarita Author Gorokhova, Svetlana Author Sheiko, Viktor Author Ohshima, Issei Author Lopez-Vaamonde, Carlos text Zootaxa 2019 2019-08-07 4652 1 1 55 journal article 26071 10.11646/zootaxa.4652.1.1 6a9d1e6c-413c-4d86-a0ed-25794f202b06 1175-5326 3363475 6A7D6858-A43D-4FD5-8B76-FE3C1EB8DAB3 Phyllonorycter sp. 5 ( cf. dakekanbae Kumata ) Material examined. Russia : SO , Sakhalin Island , Yuzhno-Sakhalinsk , Gagarin Park , 46.96N , 142.75E , 69 m alt., Betula platyphylla , 11.VII.2017 , 1 larva, NK621 , MK 403716 , deposited in INRA . Leaf mine. The mine is similar to one of Ph. ulmifoliella on Betula platyphylla ( Fig. 12B ). Pupation in the mine. Trophic specialization. Monophagous on Betula platyphylla (Betulaceae) . Distribution. Russia : RFE—SO. Remarks. BIN of unknown species—BOLD: ADM2521. In East Asia , four species of Phyllonorycter develop on Betula : Ph. cavella (Zeller) , Ph. dakekanbae (Kumata) , Ph. ulmifoliella (Hübner) , and Ph. ermani (Kumata) ( De Prins & De Prins 2018 ) . The first three species are known to colonize Betula platyphylla , the latter species develops only on B. ermanii . The nearest neighbor to Phyllonorycter sp. 5 is Ph. ulmifoliella with a genetic distance 3.0% ( Table 2 ). The other two Betula -feeding species, Ph. cavella and Ph. ermani , are genetically more divergent (>11%) comparing to Phyllonorycter sp. 5 ( Table 2 ). No DNA barcode sequences of Ph. dakekanbae exist in genetic databases. Keeping in mind that it feeds on the same host plant as Phyllonorycter sp. 5, the latter could be Ph. dakekanbae .