Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding Author Kirichenko, Natalia Author Triberti, Paolo Author Akulov, Evgeniy Author Ponomarenko, Margarita Author Gorokhova, Svetlana Author Sheiko, Viktor Author Ohshima, Issei Author Lopez-Vaamonde, Carlos text Zootaxa 2019 2019-08-07 4652 1 1 55 journal article 26071 10.11646/zootaxa.4652.1.1 6a9d1e6c-413c-4d86-a0ed-25794f202b06 1175-5326 3363475 6A7D6858-A43D-4FD5-8B76-FE3C1EB8DAB3 Phyllonorycter sp. 3 ( cf. sorbicola Kumata ) Material examined. Russia : AO, Skovorodino , nearby the train station, 53.98N , 123.93E , 431 m alt., Prunus padus , 26. VI .2016, 1 larva, NK566, MK 403696 , deposited in INRA . Leaf mine. The mine is a white elongated contracted blotch, with up to five folds on epidermis-covering mine, along the main vein. Pupation in the mine. Trophic specialization. Monophagous on Prunus padus (Rosaceae) . Distribution. Russia : RFE—AO . Remarks. BIN of unknown species—BOLD: ACY3732. In East Asia , only three Phyllonorycter species are known to develop on Prunus : Ph. cerasicolella (Herrich-Schäffer) , Ph. ringoniella (Matsumura) and Ph. sorbicola (Kumata) ( De Prins & De Prins 2018 ) . Additionally, Phyllonorycter laurocerasi Kuznetzov also develops on Prunus , but is known only from Georgia ( Kuznetzov 1979b ) and from the West Caucasian region of Russia ( Baryshnikova 2008 ). Our DNA barcoded specimen of Phyllonorycter does not match any of these three species in BOLD . No DNA barcode of Ph. laurocerasi exists in BOLD . The nearest neighbor is Ph. sorbicola , with minimal genetic distance of 2% but with different BIN ( Table 2 ). The other two species are distant from Ph. ringoniella (7.9%) and Ph. cerasicolella (10.2%) ( Table 2 ). Phyllonorycter sp. 3 represents a novel BIN in BOLD closely related to Ph. sorbicola .