Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species Author Yang, Qin Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China & The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China Author Tang, Jie Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China Author Zhou, Guo Y. Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China & The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China zgyingqq@163.com text MycoKeys 2021 2021-10-18 84 15 33 http://dx.doi.org/10.3897/mycokeys.84.71701 journal article http://dx.doi.org/10.3897/mycokeys.84.71701 1314-4049-84-15 D929207D8DC95D33AF0CBBBAE2BE14C1 Diaporthe sojae Lehman, Ann. Mo. bot. Gdn 10: 128 (1923) Figure 5 Description. Sexual morph: perithecia on pine needles in culture, black, globose, 250-500 μm in diam., densely clustered in groups, deeply immersed with elongated, tapering perithecial necks protruding through substrata, 525-800 μm . Asci unitunicate, 8-spored, sessile, elongate to clavate, (35-)37-42(-44.5) x (8-)10-11.5 μm (n = 30). Ascospores hyaline, two-celled, often 4-guttulate, with larger guttules at centre and smaller one at ends, elongated to elliptical, slightly or not constricted at septum, (9-) 9.5-11.5 x 2.7-4 μm (n = 30). Asexual morph not observed. Figure 5. Diaporthe sojae (HNZZ022) A Culture on PNA B ascomata C-E asci and ascospores. Scale bars: 500 μm ( B ); 10 μm ( C-E ). Culture characters. Culture incubated on PNA at 25 °C, originally white, fluffy aerial mycelium, reverse yellowish pigmentation developing in centre, later becoming dark brown, with yellowish-cream drops exuding from the perithecia after 15 days. Specimens examined. China . Hunan Province : Zhuzhou City , on leaves of Camellia oleifera , 27°2'41"N , 113°19'17"E , 14 Aug. 2020 , Q. Yang (USUFT 022; living cultures: HNZZ022, HNZZ008 and HNZZ010) . Notes. Diaporthe sojae was first reported on pods and stems of soybean, and subsequently reported on a wide range of hosts (Dissanayake et al. 2015; Udayanga et al. 2015 ; Guo et al. 2020 ). It was also reported on some fruit trees in China, such as Vitis spp. (Dissanayake et al. 2015) and Citrus spp. ( Huang et al. 2015 ). In the present, three isolates (HNZZ008, HNZZ010 and HNZZ022) are closely related to D. sojae in the combined phylogenetic tree (Fig. 1 ). The differences of nucleotides in the concatenated alignment (1/460 in ITS, 3/458 in cal , 1/320 in his3 and 3/433 in tub2 ) are minor. Compared with the description of the ex-type isolate FAU635, the isolate has wider asci (10-11.5 μm vs. 7-9 μm ) ( Udayanga et al. 2015 ). We therefore identify the isolates as belonging to D. sojae .