Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding) Author KNEBELSBERGER, THOMAS Author MILLER, MICHAEL A. text Zootaxa 2007 2007-07-09 1522 1 1 68 http://dx.doi.org/10.11646/zootaxa.1522.1.1 journal article 10.11646/zootaxa.1522.1.1 1175­5334 5087760 71CB20C3-57F1-46E0-AA10-58B9E64A774D Phyllodromica iberica morph #3 ( Figs 9 A–I , 10 A–H , 11 A–L , 17 ) Phyllodromica subaptera morph #3. —Knebelsberger & Bohn, 2003. Material . Numerous specimens from the following localities: Spain . Sp 148b, 186, a, b, 270, a, b, c, 271, 335, 360, a, 361, a, 363, 367, 372, 375, 380, a, 381a, 386, a, 387, a, 388, a, 389, a, 395, 446, a, 448, 449a, 451, 459, a, b, 460, 507a, 512. Description. Size. Length of pronotum: ♂ 1.44–1.64 (mean 1.56) mm; ♀ 1.78–2.05 (mean 1.91) mm. Legs. Distal end of mid tibia with 5 spines. Male Tergites. —Tergite 6 . Emargination of the posterior border similar as in morph #1 ( Figs 9 C , 11 B, D, E, F, I ). Behind the transversal ridge sometimes with structures similar to morph #2: A variably enhanced density of bristles in a line ( Fig. 11 D ) was found at the following localities: Sp 363 (in 1of 1 specimen ), Sp 375 (in 1 of 2 specimens ), Sp 395 (in 2 of 2 specimens ), Sp 449b (in 1 of 1 specimen ), Sp 451 (in 1 of 1 specimen ) and Sp 460 (in 1 of 2 specimens ). A line of bristles on a kind of torus ( Fig. 11 B, C ) was found at the localities Sp 148b (in 1of 1 specimen ), Sp 270c (in 5 of 6 specimens ), Sp 449a (in 3 of 3 specimens ), Sp 459a (in 1 of 1 specimen ) and Sp 512 (in 2 of 7 specimens ). The structures were never as strongly developed as in morph #2. Tergite 7 . Median lobe longer than in the other morphs but shorter than distance between bristle fields and the posterior border of the tergite, almost without glandular pores. Trough, mound and bristle fields similar as in morph #2: anterior wall of trough scarcely hollowed out anteriorly, medially relatively steep ( Fig. 9 D ). Mound lower and smaller than in morph #2. Bristle fields small and longitudinally oval, mostly somewhat smaller and with fewer bristles than in morph #2. Anteriorly, bristle fields immediately adjacent, rarely separated by a very narrow bristleless ridge ( Fig. 10 A ). Posteriorly, bristle fields separated by a bristleless ridge appearing as a small noselike structure as in morph #1 but considerably smaller. Bristle fields laterally limited by a bulge-like cuticular structure (white arrow heads in Fig. 10 A ). Tergite 8 . Surface scarcely elevated, with only a slight slope towards the anterior border; cone forming a variously sized membraneous bubble, sometimes relatively large ( Fig. 10 B ) but in most cases rather small and often partly invaginated (due to preparation for scanning electrone microscopy?) ( Fig.10 F, C, D ). Distance between anterior processes small ( Figs 9 E , 11 A, H, L ). Without a median gap in the distribution of the bristles in the mound region ( Fig. 10 B, F ). Tergite 10 . Supraanal plate triangular, similarly rounded as in morph #2 ( Fig. 9 G ). Sternites. —Paraproct. Medio-anterior process of right paraproct without bulge ( Fig. 9 G ). Colouration. —Tergites 2–5 . Posterior extensions of dark area weakly developed or missing ( Fig. 9 B ). Tergite 6 . Dark area variously extended, almostly reaching to the posterior border of the tergite, but always leaving at least a narrow light margin ( Fig. 9 C ), without or with lightenings especially along the longitudinal midline ( Fig. 11 D ) and in a transversal line behind the ridge ( Fig. 11 E, F, I ); in some cases dark area appearing as two transversely oval clusters of dark spots just behind the transversal ridge ( Fig. 11 E ), similar as in some specimens of morph #1. Tergite 7 . Similar to morph #2 ( Fig. 11 K ), in some cases lateral parts of the dark area less dark ( Fig. 9 D ). Tergite 8 . Dark area with light anterior projections which are much closer together than in morph #1 and enclosing a dark band ( Figs 9 E , 11 L ) or a completely isolated dark patch; sometimes the median dark marking is missing completely and the light anterior projections combine to a broad tongue ( Fig. 11 A ). Female ( Fig. 10 G, H ) For description of legs, genitalia and colouration see description of P. subaptera . Genetic Data. 1597 basepairs of the mitochondrial genome including the complete cytochrome c oxidase subunit I (COI) gene were analysed in two specimens (Sp 380a/M5, Sp 380a/M6). DNA and associated parts of body are stored in the DNATAX collection of ZSM under storage numbers DNATAX02865 (Sp 380a/M5) and DNATAX02866 (Sp 380a/M6). The sequences were submitted to GenBank under the accession numbers: AM600689 (DNATAX02865) and AM600690 (DNATAX02866). The sequences of P. iberica morph #3_Sp 380a/M5_1597bp and P. iberica morph #3_ Paratype Sp 380a/M6_1597bp differ in 6 positions (Appendix 5). The complete sequences are shown in Appendix 4. Geographical distribution ( Fig. 17 ). Morph #3 is found in two distinct areas, one covering nearly the whole north eastern part of Spain , the other covering only a very small area in the south in the western SerranÌa de Ronda. The absence of this morph in areas between is certainly not a sampling artefact since there are numerous localities in-between where other morphs had been found ( Figs 16 and 17 ). Other species of subaptera -group found . P. subaptera : Sp 148, 186, 381, 395. Other morphs of P.iberica found. Morph #1: Sp 270, 335, 367, 375, 380, 386, 388, 389, 446, 448, 449, 507, 512; morph #2: Sp 512. Remarks. At several localities remarkable variations in tergites 7 and 8 of the males have been found: At the localities Sp 186b, 270c, 459b and 512 ( Fig. 17 , morph #3 symbols labeled with an arrow) the bulge-like cuticule limitation of the bristle field on tergite 7 of some of the males appears more extended laterally (white arrows in Fig. 11 G ). In these cases the colouration of the tergite also differs: the lateral parts of the darkly coloured area appear scarcely lighter than the mound region ( Fig. 11 G ). These structures and the colouration of tergite 7 were always combined with a mostly darkly coloured tergite 8 (except for a light posterior margin) ( Fig. 11 H ). At locality 335 ( Fig. 17 , morph #3 symbol labeled with an “+”) all the males have been found with bristle fields which appear slightly larger than in the remaining specimens of morph #3 and of morph #2. The trough and the mound are well expressed. The posterior wall of the bristle fields appears more strongly hollowed out posteriorly (appearing as a crescent-shaped black shadow posteriorly of each of the two bristle fields, white arrows in Fig. 11 K ), and are more pronounced than in the remaining specimens of morph #3 and of morph #2. This formation of the structures on tergite 7 was always combined with a relatively large cone on T8 ( Fig. 11 L ). Inspite of the remarkable morphological intraspecific variability morph #3 can always be clearly distinguished from morph #2.