Two species of hackled-orb web spider genus Hyptiotes (Araneae, Uloboridae) from Guangxi, China Author Huang, Zongguang 0000-0003-1579-052X College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China & zghuang @ hunnu. edu. cn; https: // orcid. org / 0000 - 0003 - 1579 - 052 X zghuang@hunnu.edu.cn Author Yin, Haiqiang 0000-0003-0276-2407 College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China & yinhaiqiang @ hunnu. edu. cn; https: // orcid. org / 0000 - 0003 - 0276 - 2407 yinhaiqiang@hunnu.edu.cn Author Cai, Qu 0000-0001-8955-0057 College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China & cqrz @ live. cn; https: // orcid. org / 0000 - 0001 - 8955 - 0057 cqrz@live.cn Author Xu, Xiang 0000-0001-9485-5373 College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China & xux @ hunnu. edu. cn; https: // orcid. org / 0000 - 0001 - 9485 - 5373 xux@hunnu.edu.cn text Zootaxa 2023 2023-06-28 5311 1 65 84 http://dx.doi.org/10.11646/zootaxa.5311.1.3 journal article 10.11646/zootaxa.5311.1.3 1175-5326 8090355 92F1A6DD-E8D3-4F18-A78E-3D1C3F0BFDB5 Hyptiotes nonggang new species Figures 2–9 Type material: Holotype ( HNU540 ): ♁ CHINA , Guangxi Autonomous Region , Chongzuo City , Longzhou County , Nonggang National Reserve , Xiangshui Protection Station , 22°26.13'N , 107°1.43'E , 262m, 1 XI, 2017, Ailan He , Keke Liu , Qu Cai , Jihe Liu , Jinxin Liu and Zongguang Huang leg. Paratypes: 1♀ ( HNU541 ), the same collecting information as holotype; 3♁ 3♀ ( HNU542–547 ) Nonggang National Reserve , Nonggang Protection Station , 22°27.84'N , 106°55.95'E , 228m, 26 X, 2017, collected by the same people as above . Etymology. The species epithet is derived from the type locality, Nonggang National Reserve, Guangxi , China . Diagnosis. The male of the new species resembles that of Hyptiotes dentatus Wunderlich 2008 in having two tegular apophyses, and a very long embolus circling around the bulb for more than one circle but can be distinguished from each other by different positions of origin of embolus and different shapes of tegular apophyses 2 (TE2) and terminal apophyses (TA): embolus approximately originated from the seven o’clock and so can be seen in the prolateral view of palpal organ in the new species ( Figs 4A , 5A ), while embolus approximately originated at the nine o’clock (the right palp described in Wunderlich, 2008 ) and can be seen in the prolateral view of palpal organ in H. dentatus ( Wunderlich 2008 : fig. 9); TE2 extended flat, extending transversely in the new species ( Figs 4C , 5C ), while TE2 slender, extending longitudinally in H. dentatus ( Wunderlich 2008 : figs 8, 9); TA highly sclerotized, like an macrosetae in the new species ( Figs 4C , 5C ), while TA wide and flat, membranous and translucent in H. dentatus ( Wunderlich 2008 : figs 8, 9). The male of the new species also resembles that of H . paradoxus but can be distinguished easily from each other by median apophysis (MA) and TE2: MA with a wide apical part in new species ( Figs 4C , 5C ), while with a narrow apical part in H. paradoxus ( Figs 12C , 13C ). TE2 with only one end in new species ( Figs 4C , 5C ), while with two ends in H. paradoxus ( Figs 12C , 13C ). The female of the new species resembles that of Hyptiotes gerhardti Wiehle 1929 in having similar receptacula (R), and long and twisted copulatory ducts (CD), but can be distinguished from each other by the number of loops of accessory glands (AG) and the developed degrees of median ridge (MR), AG twisted to form at least 7 regular loops around receptaculum in the new species ( Figs 8B , 9B ), while AG twisted, but not to form regular loops in H . gerhardti ( Wunderlich 2017 : fig. 14). MR weak in the new species ( Fig. 8C ), while MR developed in H . gerhardti ( Wunderlich 2017 : figs 13, 14). The obvious difference between the females of new species and H . paradoxus is that MR of the new species is inconspicuous ( Fig. 8C ), while that of the latter is distinct ( Fig. 16C ). FIGURE 2. Hyptiotes nonggang n. sp. , holotype, male, habitus. A, dorsal view; B, ventral view; C, lateral view. The red arrow pointing to the longitudinal strip on the clypeus strip on the clypeus. Scale bars: A–C, 0.5 mm. FIGURE 3. Hyptiotes nonggang n. sp. , holotype, male. A, clypeus, frontal view; B, cribellum and spinnerets, ventral view; C, femur III, retrolateral view; D, metatarsus and tarsus IV, retrolateral view. The red arrow pointing to the longitudinal strip on the clypeus. Scale bars: A, 0.2 mm; B–D, 0.1 mm. Description. Male . Total length 2.83, carapace 1.08 long, 1.13 wide, abdomen 2.58 long, 2.04 wide. Carapace deep brown, nearly triangular, narrow anteriorly, and sparsely covered with plumose setae. Clypeus slightly light in color, with two brown longitudinal strips in front of ALE (The position pointed by the red arrows in Figs 2A , 3A ). Eye area strongly elevated. Fovea wide and deep. Eight eyes are present, but ALE degenerated, only dark red marks remained. Posterior border of MOA about six times of the anterior border in width. Eye diameters: AME 0.06, PME 0.12, PLE 0.13, AME-AME 0.02, AME-PME 0.22, PME-PME 0.56, PME-PLE 0.31, PLE-PLE 0.91 ( Figs 2A, C ; 3A ). Endite almost as long as wide. Labium triangular. Sternum yellow, shield-shaped, with a posterior hump ( Fig. 2B ). Legs brown dorsally and yellowish ventrally, bearing many strong bristles ( Fig. 2A, B ). Femur III with a row of trichobothria dorsally and metatarsus IV with calamistrum dorsally ( Fig. 3C, D ). Leg formula 1423. Leg measurements: I 3.49 (1.18, 0.36, 0.81, 0.84, 0.3); II 2.44 (0.87, 0.31, 0.32, 0.67, 0.27); III 2.06 (0.67, 0.21, 0.31, 0.53, 0.34); IV 2.70 (0.83, 0.37, 0.55, 0.67, 0.28). Abdomen oval, light yellow, covered with short setae and plumose setae, and dorsally with irregular dark spots and two rows of white dots in the middle line; ventrally, anterior spinnerets much larger than others. Cribellum wide, close to spinnerets. ( Figs 2B, C ; 3B ). Palp ( Figs 4 , 5 ). Terminal apophysis sclerotized and bending towards prolateral side. Median apophysis strong, highly sclerotized, with the apical part rolled towards both base and venter. Tegular apophysis 1 membranous, transversely extending and slightly twisting. Tegular apophysis 2 strong sclerotized, transversely extending, with a slender apical end bending towards base. Conductor consisting of two parts: one part with a strong sclerotized apical part; the other part membranous, gradually becoming narrow. Embolus extremely long, with the original point be seen in the prolateral side. Cymbium long and narrow, with numerous of long setae. Patella and tibia with a strong bristle each and tibia also with a tuft of neat and long setae. FIGURE 4. Hyptiotes nonggang n. sp. , holotype, palp of male. A, prolateral view; B, retrolateral view; C, ventral view. Abbreviations: C, stronger sclerotized part of the conductor; E, embolus; MA, median apophysis; O, origin of the embolus; TC, translucent and wide part of the conductor; TA, terminal apophysis; TE1, tegular apophysis 1; TE2, tegular apophysis 2. Scale bars: A–C, 0.5 mm. FIGURE 5. Hyptiotes nonggang n. sp. , holotype, palp of male. A, prolateral view; B, retrolateral view; C, ventral view. Abbreviations: C, stronger sclerotized part of the conductor; E, embolus; MA, median apophysis; O, origin of the embolus; TC, translucent and wide part of the conductor; TA, terminal apophysis; TE1, tegular apophysis 1; TE2, tegular apophysis 2. Scale bars: A–C, 0.5 mm. FIGURE 6. Hyptiotes nonggang n. sp. , paratype, female, habitus. A, dorsal view; B, ventral view; C, lateral view. Scale bars: A–C, 0.5 mm. FIGURE 7. Hyptiotes nonggang n. sp. , holotype, female. A, clypeus, frontal view; B, cribellum and spinnerets, ventral view. Scale bars: A, B, 0.2 mm. Female : Total length 3.25, carapace 1.14 long, 1.17 wide, abdomen 2.58 long, 2.04 wide. Carapace light brown. Eye diameters: AME 0.08, PME 0.13, PLE 0.2, AME-AME 0.05, AME-PME 0.21, PME-PME 0.76, PME-PLE 0.13, PLE-PLE 1.2 ( Figs 1A, C ; 2A ). Leg measurements: I 3.49 (1.18, 0.36, 0.81, 0.84, 0.3); II 2.44 (0.87, 0.31, 0.32, 0.67, 0.27); III 2.06 (0.67, 0.21, 0.31, 0.53, 0.34); IV 2.70 (0.83, 0.37, 0.55, 0.67, 0.28). The other features of cephalothorax and legs resemble those of the male ( Figs 6 , 7A ). Abdomen oval, with middle area projecting upwards ( Fig. 6C ). White patches covering throughout abdomen ( Fig. 6 ). Anterior spinnerets much larger than others. Cribellum close to spinnerets and relatively larger than that of male ( Figs 6B , 7B ). Epigynum ( Figs 8 , 9 ): Epigynum posterior face plate lightly sclerotized with an inconspicuous median ridge (MR). Copulatory opening long and narrow. Copulatory duct sclerotized and twisted. Accessory glands twisting around the receptaculum to form seven loops. Receptaculum spherical. Distribution. China ( Guangxi ) ( Fig. 18 )