New Neotropical species of Downeshelea Wirth and Grogan and redescription of D. multilineata (Lutz) (Diptera: Ceratopogonidae) Author Santarém, Maria Clara Alves Author Borkent, Art Author Spinelli, Gustavo Author Felippe-Bauer, Maria Luiza text Journal of Natural History 2018 J. Nat. Hist. 2018-03-07 52 9 - 10 509 540 http://dx.doi.org/10.1080/00222933.2018.1437231 journal article 10.1080/00222933.2018.1437231 1464-5262 5178489 9FC2A016-1295-4047-877E-BC9030BD2BD2 Downeshelea multilineata species group: new group Abbreviated description . Small- to medium-sized species (male wing length 0.85–1.37 mm , female wing length 0.92–1.30 mm ), brown in colouration. Male eyes narrowly separated by a distance shorter than one ommatidia or contiguous in lower area; female eyes contiguous. Male antenna pale, except base of flagellomere 1, distal portion of flagellomere 10 and flagellomeres 11–13 brown; female antenna brown, basal portion of flagellomeres 1–7 pale; flagellomeres 2–8 somewhat barrel-shaped, 9–13 cylindrical, elongate. Palpus brown, segment 3 swollen or slightly swollen on midportion, with broad, deep sensory pit. Male mandible unarmed, female mandible with 9–12 teeth. Legs brown or pale brown, hind leg darker (except in D. casimirensis ), hind tibial comb with six spines; apical spines of male hind tarsomeres 2–4: 1–1–1; male claws about 0.35–0.45 length of their respective tarsomere 5; female hind leg claw 0.89–1.53 length of tarsomere 5. Wing hyaline, with dark bristles on costa; two distinctive black spots, one on r-m, other in r 3 from apex of r 2 to M 1 ; three distinctive greyish areas: first near apex of M 1 , second sigmoid-shaped near apex of M 2 extending to wing margin in m 2 , third over CuA 2 , also in CuA 1 in some species. Male tergite 9 gradually tapering distally, distal ½ with sclerotised band laterally; gonocoxite moderately stout; gonostylus nearly straight, proximal ½ slightly pilose; parameres fused on basal portion, each with trilobed basal arm, heavily sclerotised; stem stout, straight or sinuous; distal portion abruptly bent ventrally, directed anteromesally, tapering to pointed tip, overlapped in some species; aedeagus subtriangular to rectangular, posterior portion with mesal excavation. Female with spermathecae variable in size, with short sclerotised ducts, third rudimentary spermathecae generally present. Key to males of the Downeshelea multilineata species group 1. Legs brown or pale brown, without bands (although some with varying pigmentation); wing with three distinctive greyish areas (in addition to the two dark spots on r-m and in r 3 from the apex of r 2 to M 1 ) ( Figure 1a ); parameres distal portion abruptly bent ventrally, directed anteromesally, tapering to pointed tip, 0.50–0.75 of total paramere length .................................................. multilineata species group.................................................. 2 – Legs various; wing with variable distinctive greyish areas; parameres distal portion not as above ........................................................................................ other Downeshelea species 2. Legs brown, hind femur brown; medium- to large-sized species; midtarsomere 1 with seven to eight ventral spines. Parameres with a short posteromedian projection on the medial fused portion................................................................................................................................ 3 – Legs brown or pale brown, hind femur darker subapically; small- to medium-sized species; midtarsomere 1 with two to six ventral spines. Parameres without a posteromedian projection on the medial fused portion....................................................................... 4 3. Tergite 9 with short, broad apicolateral process; paramere stem sinuous on midportion, expanded subapically forming a broad lobe laterally directed; distal portion 0.55– 0.84 of total paramere length ( Figure 8i ); aedeagus without elliptical sclerotised anterior areas, distal portion terminating in sclerotised non-serrate process ( Figure 8h ) ..................................................................................................................................... D. eclectica sp. nov. – Tergite 9 with long, slender apicolateral process; paramere stem sinuous, proximal 2/3 directed posterolaterally, distal 1/3 slender directed posteromesally; distal portion 0.50 of total paramere length ( Figure 9i ); aedeagus with two elliptical sclerotised anterior areas; distal portion terminating in sclerotised serrate processes ( Figure 9h ).................... ...................................................................................................................................... D. moravia sp. nov. 4. CuA 1 pale; paramere distal portion short, not reaching the medial fused portion, 0.53– 0.62 of total paramere length ............................................................................................................ 5 – CuA 1 greyish; paramere distal portion elongate, reaching the medial fused portion, 0.62–0.80 of total paramere length .................................................................................................. 7 5. Legs pale brown, foretibia pale or darker apically; halter knob pale; aedeagus subtriangular, with or without sclerotised anteromesal areas, not reaching midlength when present; basal arch extending to 0.22–0.56 of total length....................................... 6 – Legs brown ( Figure 7d ); halter knob brown; aedeagus rectangular, with two large elliptical sclerotised anteromesal areas reaching midlength; basal arch extending to 0.17 of total length ( Figure 9f )................................................................ D. marambaia sp. nov. 6. Hind tibia pale ( Figure 2d ); gonostylus 0.81 length of gonocoxite ( Figure 8c ); parameres stem straight, gradually swollen to apex; distal portion deeply curved ( Figure 8e ); aedeagus with pair of submedian sclerotised stripes and oval areas, basal arch extending to 0.56 of total length ( Figure 8d ).................................................... D. casimirensis sp. nov. – Hind tibia darker apically and on proximal third ( Figure 5c ); gonostylus 0.58–0.64 length of gonocoxite ( Figure 9a ); parameres stem basolaterally expanded, more slender and convergent distally, distal portion nearly straight ( Figure 9b ); aedeagus without sclerotised stripes and oval areas, basal arch extending to 0.22–0.28 of total length ( Figure 9a ) .................................................................................................... D. jarina sp. nov. 7. Sternite 9 with large prominent convex median lobe ( Figure 8a ); paramere stem straight, distal portion slightly curved ( Figure 8b ); aedeagus subtriangular, with two elliptical sclerotised anterior areas ( Figure 8a ) .............................................................. D. multilineata (Lutz) – Sternite 9 with moderately convex median lobe; paramere stem and distal portion various; aedeagus rectangular or subrectangular, without elliptical sclerotised anterior areas ............................................................................................................................................................... 8 8. Halter knob pale; midtibia paler basally ( Figure 3c ); paramere stem broad, sinuous, expanded apicolaterally, distal portion gradually curved ( Figure 8g ); aedeagus subrectangular, basal arch extending to 0.22–0.26 of total length ( Figure 8f ).......................... ............................................................................................................................. D. costaricensis sp. nov. – Halter knob brown; midtibia brown ( Figure 6c ); paramere stem nearly straight basally, expanded distally in inner portion, distal portion anteromesally directed abruptly curved to tip ( Figure 9d ); aedeagus rectangular, basal arch extending to 0.34–0.42 of total length ( Figure 9c )......................................... ......................................... D. litorale sp. nov. Key to females of the Downeshelea multilineata species group Female members of the multilineata group can only be recognised as such if associated with males of the group. The females of D. moravia , D. marambaia and D. casimirensis are unknown. 1. Legs pale brown; hind tibia darker apically and on proximal third..... ..... D. jarina sp. nov. – Legs brown; hind tibia dark brown.................................................................................................. 2 2. Halter pale; mandible with 10 teeth......................... ......................... D. costaricensis sp. nov. Halter brown; mandible with 11–12 teeth.................................................................................... 3 3.Midtibia paler basally ............................................ ............................................ D. multilineata Lutz – Midtibia brown ........................................................................................................................................... 4 4.Hind femur brown; midtarsomere 1 with six to nine ventral spines .................................. ..................................................................................................................................... D. eclectica sp. nov. – Hind femur darker subapically; midtarsomere 1 with four to five ventral spines............. ......................................................................................................................................... D. litorale sp. nov.