Diversity of Cladosporium (Cladosporiales, Cladosporiaceae) species in marine environments and report on five new species Author Lee, Wonjun https://orcid.org/0000-0002-7227-0777 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea Author Kim, Ji Seon https://orcid.org/0000-0003-1869-7347 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea Author Seo, Chang Wan https://orcid.org/0000-0002-5948-1836 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea Author Lee, Jun Won https://orcid.org/0000-0003-1416-3428 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea Author Kim, Sung Hyun https://orcid.org/0000-0002-5505-9451 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea Author Cho, Yoonhee https://orcid.org/0000-0002-0743-0914 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea Author Lim, Young Woon https://orcid.org/0000-0003-2864-3449 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea ywlim@snu.ac.kr text MycoKeys 2023 2023-06-02 98 87 111 http://dx.doi.org/10.3897/mycokeys.98.101918 journal article http://dx.doi.org/10.3897/mycokeys.98.101918 1314-4049-98-87 A4D48209585156F58E1CEB8CBA872D78 Cladosporium marinum Wonjun Lee & Y.W. Lim sp. nov. Figs 2D , 3D Typification. Republic of Korea. Jeju-do, Chuja-myeon, 33°23′53″N , 126°14′24″E , seaweed, 31 Aug 2021, M.S. Park & Y.W. Lim (holotype SFC20230103-M33, stored in a metabolically inactive state). Etymology. The name ' marinum ', derived from Latin, refers to the various marine substrates (seaweed, sea sand, and mudflat) from which the species was isolated. Description. Asexual morphology: Mycelium superficial and immersed, composed of septate, branched, hyaline to subhyaline, smooth to verruculose hyphae, nodulose, 2-5.7 μm wide. Conidiophores macronematous and micronematous, arising laterally or terminally from hyphae, (sometimes reduced to conidiogenous cells), septate, slightly flexuous, non-nodulose, usually unbranched or branched, up to 243 μm long, 2-4 μm wide, pale brown, verruculose to verrucose. Conidiogenous cells integrated, terminal and intercalary, cylindrical to subcylindrical, 20.5-47.7 x 2-3.6 μm , bearing up to four slightly darkened and refractive conidiogenous loci. Ramoconidia 0-1(-2)-septate, subcylindrical to cylindrical, 10.2-28.171 x 2.4-4.1 μm [av. ( +/- SD) 17.3 ( +/- 2.39) x 2.9 ( +/- 0.37)], pale brown, verruculose. Conidia forming branched chains, with up to five conidia in the terminal unbranched part, long neck area between conidia, aseptate, pale brown, smooth to verruculose, with protuberant, slightly darkened and refractive hila. Small terminal conidia aseptate, obovoidal to ellipsoidal, 2.9-5.3 x 2-3 μm [av. ( +/- SD) 3.8 ( +/- 0.53) x 2.5 ( +/- 0.25)]. Intercalary conidia aseptate, very rarely 1-septate, ellipsoidal to limoniform, obovoid, sometimes subcylindrical, 2.7-6.2 x 1.8-2.8 μm [av. ( +/- SD) 4.2 ( +/- 0.76) x 2.3 ( +/- 0.22)]. Secondary ramoconidia 0-1-septate, subcylindrical to cylindrical, slightly obclavate, 8.5-28.2 x 2.3-3.7 μm [av. ( +/- SD) 14.5 ( +/- 4.38) x 2.9 ( +/- 0.35)]. Cultural characters : Colonies on PDA 37-66 mm diam after 14 d at 25 °C, olive (3E3 to 3F4), reverse dark gray (1F1), floccose-velvety, sometimes woolly, raised, radially furrowed, wrinkled, raised; margin white, slightly hyaline edge, undulated; aerial mycelia abundantly formed, with numerous prominent exudates, sporulation profuse. Colonies on MEA 35-40 mm diam after 14 d at 25 °C, grayish white (1B1) to olive gray (3D2), reverse dark gray (1F1), velvety, raised to crateriform, radially furrowed at the margin region, wrinkled; margin white, regular; aerial mycelia abundantly formed, dense, without prominent exudates, sporulation profuse. Colonies on OA 41-47 mm diam after 14 d at 25 °C, gray (1C1 to 1E1), reverse concolorous, velvety, floccose, raised, radially furrowed; margin yellowish, regular; aerial mycelia abundantly formed, with few exudates, sporulation profuse. Colonies on SNA 37-41 mm diam after 14 d at 25 °C, olive (3D3) to olive yellow (3E8), reverse olive (3D3 to 3F3), slightly fluffy-floccose, powdery, flat; margin yellowish white (2A2), hyaline, undulate; aerial mycelia abundantly moderate, without prominent exudates, sporulation profuse. Habitat and distribution. Isolated from mudflats, sea sands, and seaweeds; Southern and Western Korean seaside in Republic of Korea. Additional cultures examined. Republic of Korea. Jeju-do, Chuja-myeon, 33°23′53″N , 126°14′24″E , seaweed, 31 Aug 2021, M.S. Park & Y.W. Lim (SFC20230103-M30, stored in a metabolically inactive state); Jeju-do, Chuja-myeon, 33°23′53″N , 126°14′24″E , seaweed, 31 Aug 2021, M.S. Park & Y.W. Lim (SFC20230103-M31, stored in a metabolically inactive state); Jeju-do, Chuja-myeon, 33°23′53″N , 126°14′24″E , seaweed, 31 Aug 2021, M.S. Park & Y.W. Lim (SFC20230103-M32, stored in a metabolically inactive state); Jeju-do, Chuja-myeon, 33°57′50″N , 126°17′38″E , seaweed, 31 Aug 2021, M.S. Park & Y.W. Lim (SFC20230103-M34, stored in a metabolically inactive state); Jeju-do, Hallim-eup, 33°23′53″N , 126°14′24″E , seaweed, 15 Aug 2021, M.S. Park & Y.W. Lim (SFC20230103-M35, stored in a metabolically inactive state); Jeju-do, Hallim-eup, 33°23′53″N , 126°14′24″E , seaweed, 15 Aug 2021, M.S. Park & Y.W. Lim (SFC20230103-M36, stored in a metabolically inactive state); Jeollanam-do, Muan-gun, 35°01′40″N , 126°25′16″E , mudflat, Apr 2017, M.S. Park & Y.W. Lim (SFC20230103-M37, stored in a metabolically inactive state); Jeollanam-do, Muan-gun, 35°03′44″N , 126°20′13″E , sea sand, Oct 2016, M.S. Park & Y.W. Lim (SFC20230103-M38, stored in a metabolically inactive state); Jeollanam-do, Suncheon-si, 34°50′29″N , 127°29′09″E , sea sand, Jul 2020, M.S. Park & Y.W. Lim (SFC20230103-M39, stored in a metabolically inactive state); Jeju-do, Seogwipo-si, 33°13′58″N , 126°18′47″E , sea sand, Jan 2021, M.S. Park & Y.W. Lim (SFC20230103-M40, stored in a metabolically inactive state). Notes. Cladosporium marinum sp. nov. forms an independent clade in the phylogenetic tree of the C. cladosporioides species complex (Fig. 1 ), but it has no distinguishable characteristics compared to those of the other species. Molecular and phylogenetic approaches are imperative to identify and differentiate this species from other species.