Tylopilus pallidocervicolor, a new species from Baiyun Mountain, Guangzhou, China Author Lin, Zhi-Jun 0000-0002-3604-828X State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China & linzhj 36 @ mail 2. sysu. edu. cn; https: // orcid. org / 0000 - 0002 - 3604 - 828 X linzhj36@mail2.sysu.edu.cn Author Li, Hui-Bin 0009-0007-9415-8456 State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China & lihb 37 @ mail 2. sysu. edu. cn; https: // orcid. org / 0009 - 0007 - 9415 - 8456 lihb37@mail2.sysu.edu.cn Author Liu, Xiao-Chun 0009-0000-3449-6268 State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China & liuxch 67 @ mail 2. sysu. edu. cn; https: // orcid. org / 0009 - 0000 - 3449 - 6268 liuxch67@mail2.sysu.edu.cn Author Liang, Ying-Shan 0000-0001-6001-4752 State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China & liangysh 37 @ mail 2. sysu. edu. cn; https: // orcid. org / 0000 - 0001 - 6001 - 4752 liangysh37@mail2.sysu.edu.cn Author Huang, Xiao-Xia 0000-0002-4898-1018 State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China & huangxx 83 @ mail 2. sysu. edu. cn; https: // orcid. org / 0000 - 0002 - 4898 - 1018 huangxx83@mail2.sysu.edu.cn Author Qiu, Li-Hong 0000-0001-9930-292X State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China qiulh@mail.sysu.edu.cn text Phytotaxa 2023 2023-10-06 619 2 137 151 http://dx.doi.org/10.11646/phytotaxa.619.2.2 journal article 10.11646/phytotaxa.619.2.2 1179-3163 8425871 Tylopilus pallidocervicolor Z.J. Lin & L.H. Qiu , sp. nov. ( Figs. 3 , 4 ) MycoBank: MB 848015 Etymology:—“ pallido ” (Lat.)—pale; “ cervicolor ” (Lat.)—deer color, referring to the color of the basidiomata. Holotype :— CHINA , GUANGDONG PROVINCE : Guangzhou City , Baiyun Mountain , 23°10′10″ N 113°17′31″ E , elev. 120 m , 20 August 2022 , L.H. Qiu , GDGM91094 (GenBank acc. no.: OQ617298 for nrITS, OQ617304 for nrLSU, OQ676968 for rpb1 , OQ676973 for rpb2 , OQ676977 for tef1-α ). FIGURE 1. A phylogram of Tylopilus pallidocervicolor inferred from a multilocus (nrLSU, rpb1 , rpb2 and tef1-α ) dataset using maximum likelihood and Bayesian inference. BS ≥ 70% and PP ≥ 0.95 are indicated above or below the branches as BS/PP. The new taxon is shown in bold. Scale bar: 0.01 substitutions. FIGURE 2. A phylogram of Tylopilus pallidocervicolor based on nrLSU dataset using maximum likelihood and Bayesian inference. BS ≥ 70% and PP ≥ 0.95 are indicated above or below the branches as BS/PP. The new taxon is shown in bold. Scale bar: 0.01 substitutions. Diagnosis:— Tylopilus pallidocervicolor is characterized by tannish brown to light brown basidiomata, stipe with distinct reticulum on upper portion, and a palisadoderm pileipellis. Description:— Basidiomata medium to large-sized. Pileus (3.5) 5–11 cm in diam., hemispherical to subhemispherical when young, applanate to plano-depressed at maturity; surface dry, finely tomentose, reddish brown (#8C3613) to dark brown (#704214) when young, tannish brown (#D2B48C) to light brown (#AC8260) at maturity, paler towards margin with age; context solid when young, spongy with age, white, without discoloration when bruised. Hymenophore poroid, adnate when young, slightly depressed around the apex of stipe with age; surface yellowish brown (#AD8F77) to pinkish brown (#E3B7AC), staining nude (#BF7258) when bruised; pores angular, 0.6–1.1 mm wide; tubes 6–14 mm long, concolorous with hymenophoral surface, slowly staining deep brown (#8E5627) when bruised or aging. Stipe 4–6.8 × 2.1–2.3 cm , clavate to subcylindrical, moderately enlarged downwards when young, attenuate at middle at maturity; surface predominantly concolorous with pileus, distinctly reticulate over upper portion, slowly staining brownish red when touched; context solid, pallid, slowly staining brown (#8C794E, #5A3E29) when bruised; basal mycelium pallid to yellowish. Spore print not obtained. Taste not obtained. Odor mild. FIGURE 3. Tylopilus pallidocervicolor : a–f. Basidiomata (a–c: GDGM91091; d–g: GDGM91094, holotype); h. Scanning electron micrograph of basidiospores (GDGM91094, holotype). Photos: a–b, d–g: L.H. Qiu; c: X.X. Huang; h: Z.J. Lin. Scale bars: b–c, e–g = 1 cm; h = 1 μm. FIGURE 4. Microscopic features of Tylopilus pallidocervicolor (GDGM91094, holotype): a. Basidiospores; b. Cheilocystidia; c. Pleurocystidia; d. Basidia; e. Terminal cells of pileipellis. Scale bars: a–e = 10 μm. Drawings by Z.J. Lin. Basidiospores [200/3/3] (8.8) 10.1–12.0 (15.0) × (3.1–)3.5–4.1 (4.8) μm, Q = (2.39–)2.66–3.23 (–3.51), Q m = 2.94 ± 0.22, ellipsoid to cylindrical, smooth under light microscope and SEM, hyaline to yellowish brown in KOH, yellowish brown in Melzer’s reagent. Basidia 22–29 × 7–10 μm, clavate, 4-sterigmata, hyaline in KOH. Hymenophoral trama boletoid, composed of 8–11 μm wide cylindrical hyphae, thin-walled, hyaline in KOH. Cheilocystidia moderate, scattered, 43–56 × 8–13 μm, ventricose to ventricose-mucronate, thin-walled, yellowish brown in KOH. Pleurocystidia rare, scattered, 64–78 × 7–14 μm, fusiform to subfusiform, thin-walled, yellowish brown in KOH. Pileipellis a palisadoderm, composed of 6–10 wide μm hyphae, thin-walled, hyaline or yellowish in KOH, yellowish brown in Melzer’s reagent; terminal cells 24–54 (64) × 9.0–16.1 (20) μm, fusiform to subfusiform or ventricose, thin-walled, hyaline or yellowish to brownish in KOH. Pileal trama composed of 5–12 μm wide interwoven cylindrical hyphae, thin-walled, hyaline in KOH. Stipitipellis a cutis about 236 μm thick, composed of 3–7 μm wide cylindrical hyphae, thin walled, yellowish to brownish in KOH. Stipe trama composed of cylindrical hyphae 5–8 μm wide, thin-walled, hyaline in KOH. Clamp connections absent in all tissues. Habitat:—Scattered to solitary on soil in subtropical forests dominated by Fabaceae and Fagaceae . Distribution:—Currently known from southern China . Additional specimens examined:— CHINA , GUANGDONG PROVINCE : Guangzhou City , Baiyun Mountain : 23°10′7″ N 113°17′31″ E , elev. 90 m , 5 June 2021 , GDGM91091 , L.H. Qiu ; 23°10′7″ N 113°17′31″ E , elev. 119 m , 12 June 2021 , B21061201 , X.X. Huang ; 23°10′3″ N 113°17′32″ E , elev. 88 m , 16 August 2021 , GDGM91092 , L.H. Qiu ; 23°10′3″ N 113°17′29″ E , elev. 88 m , 4 September 2021 , GDGM91093 , Z.J. Lin & Y.S. Liang .