A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles bobhanneri Arias-Penna, sp. nov. Figs 31 , 32 Female. Body length 2.53 mm , antenna length 2.98 mm , fore wing length 2.78 mm . Type material. Holotype : COSTA RICA • 1♀ ; 04-SRNP-33819, DHJPAR0001511]; Area de Conservacion Guanacaste , Guanacaste , Sector Pitilla , Sendero Mismo ; rain forest; 680 m ; 10.98758 , -85.41967 ; 01.vii.2004 ; Manuel Rios leg.; caterpillar collected in fourth instar; bud-like cocoons, scattered loose, adhered to the leaf substrate and formed on 11.vii.2004 ; adult parasitoids emerged on 17.vii.2004 ; ( CNC ) . Paratypes . • 3 ( 1♀ , 1♂ ) ( 1♀ , 0 ♂ ); 04-SRNP-33819, DHJPAR0001511; same data as for holotype; ( CNC ) . Other material. Reared material. COSTA RICA : Area de Conservacion Guanacaste , Guanacaste , Sector Pitilla , Sendero Mismo : • 3 ( 2♀ , 0 ♂ ) ( 1♀ , 0 ♂ ); 04-SRNP-33811, DHJPAR0001473; rain forest; 680 m ; 10.98758 , -85.41967 ; 01.vii.2004 ; Petrona Rios leg. ; caterpillar collected in fourth instar; white ridged bud-like cocoons adhered to the leaf substrate and formed on 09.vii.2004 ; adult parasitoids emerged on 17.vii.2004 . • 5 ( 2♀ , 0 ♂ ) ( 3♀ , 0 ♂ ); 04-SRNP-33810, DHJPAR0001502; same data as for preceding except: scattered white bud-like cocoons adhered to the leaf substrate and formed on 06.vii.2004 ; adult parasitoids emerged on 14.vii.2004 . • 3 ( 1♀ , 1♂ ) ( 1♀ , 0 ♂ ); 04-SRNP-33813, DHJPAR0001515; same data as for preceding except: caterpillar collected in third instar; white bud-like cocoons formed on 06.vii.2004 ; adult parasitoids emerged on 14.vii.2004 . • 2 (0 ♀ , 1♂ ) (0 ♀ , 1♂ ); 04-SRNP-33808, DHJPAR0001518; same data as for preceding except: white bud-like cocoons formed on 06.vii.2004 ; adult parasitoids emerged on 14.vii.2004 . • 2 ( 1♀ , 0 ♂ ) ( 1♀ , 0 ♂ ); 04-SRNP-33648, DHJPAR0001525; same data as for preceding except: 23.vi.2004 ; Manuel Rios leg. ; caterpillar collected in third instar; white bud-like cocoons formed on 22.vii.2004 and adhered to the leaf substrate; adult parasitoids emerged on 28.vii.2004 . Area de Conservacion Guanacaste, Guanacaste , Sector Pitilla , Sendero Laguna ; • 3 ( 2♀ , 0 ♂ ) ( 1♀ , 0 ♂ ); 04-SRNP-33351, DHJPAR0001531; rain forest; 680 m ; 10.9888 , -85.42336 ; 15.vi.2004 ; Calixto Moraga leg. ; caterpillar collected in third instar; white bud-like cocoons adhered to the leaf substrate and formed on 27.vi.2004 ; adult parasitoids emerged on 09.vii.2004 . Area de Conservacion Guanacaste, Guanacaste , Sector Pitilla , Estacion Quica : • 2 ( 1♀ , 0 ♂ ) ( 1♀ , 0 ♂ ); 08-SRNP-71182, DHJPAR0031102; rain forest; 470 m ; 10.99697 , -85.39666 ; 07.vii.2008 ; Oscar Siezar leg. ; caterpillar collected in third instar; white bud-like cocoons adhered to the leaf substrate and formed on 29.vii.2008 ; adult parasitoids emerged on 06.viii.2008 . Diagnosis. Precoxal groove smooth and shiny ( Figs 31A, G, H , 32A ), medioanterior pit of metanotum circular without median longitudinal carina ( Figs 31G , 32G ), inner margin of eyes straight throughout, scutellar punctation indistinct throughout ( Figs 31G , 32G ), fore wing with 1 cu-a vein complete, touching the edge of 1-1A vein, outer side of junction of r and 2RS veins not forming a stub ( Figs 31L , 32L ), propodeum with a median longitudinal carina ( Figs 31G , 32G ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Figs 31H , 32K ). Figure 31. Glyptapanteles bobhanneri sp. nov. female 04-SRNP-33819 DHJPAR0001511 A Habitus B, C Head B Laterofrontal view C Dorsal view D Flagellomeres E Hind coxa, lateral view F Genitalia: hypopygium, ovipositor sheaths, lateral view G, H, J Mesosoma G Dorsolateral view H Ventrolateral view J Lateral view I T1-2, dorsolateral view K Metasoma, lateral view L, M Wings L Fore M Hind. Figure 32. Glyptapanteles bobhanneri sp. nov. male 04-SRNP-33819 DHJPAR0001511 A Habitus B, C Head B Laterofrontal view C Dorsal view D Flagellomeres E Hind coxa, lateral view F Genitalia: parameres, lateral view G, H, J Mesosoma G Dorsolateral view H Ventrolateral view J Lateral view I T1-2, dorsal view K Metasoma, lateral view L, M Wings L Fore M Hind. Coloration ( Fig. 31A ). General body coloration dark brown black except labrum, mandibles, glossa, maxillary and labial palps, and tegulae yellow-brown. Eyes silver and ocelli silver/yellowish. Fore and middle legs yellow-brown, fore claws brown; hind legs yellow-brown except coxae brown-black with apex yellow-brown, apex of femora, both ends of tibiae brown and tarsomeres brown although tarsomeres 1-3 with a proximal yellow-brown ring. Petiole on T1 black-brown and sublateral areas yellow-brown; T2 with median area brown with contours darkened, adjacent area narrow and brown, and lateral ends yellow-brown; T3 and beyond yellow-brown/brown; distally each tergum with a narrow whitish band. In lateral view, T1-2 completely yellow; T3 and beyond yellow, but dorsally brown, extent of brown area remains constant in each tergum. All sterna yellow, but hypopygium medially brown and some lateral parts too; ovipositor sheaths brown. Description. Head ( Fig. 31 A-D ). Head triangular with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.25:0.08, 0.25:0.08, 0.24:0.08), distal antennal flagellomere longer than penultimate (0.12:0.06, 0.10:0.05), antenna longer than body (2.98, 2.53); antennal scrobes-frons shallow. Face convex with scattered finely punctate, interspaces smooth, and longitudinal median carina present. Frons smooth. Temple wide, punctate, and interspaces clearly smooth. Inner margin of eyes straight throughout; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.12). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally rounded and dorsally wide. Mesosoma ( Fig. 31A, G, H, J ). Mesosoma dorsoventrally convex. Mesoscutum convex, punctation distinct throughout, and interspaces smooth. Scutellum long and slender, apex sloped and fused with BS , scutellar punctation indistinct throughout, scutellum in profile flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM ; ATS demilune with a little and complete parallel carinae; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae; area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM upward; MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM ; PFM thick and smooth; ATM proximally with little and incomplete parallel carinae. Propodeum with a clearly visible median longitudinal carina, proximal half straight or nearly so, with fine sculpture and distal half rugose with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and short stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron finely sculptured only ventrally and dorsally without a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge convex, teardrop-shaped. Legs ( Fig. 31A, E ). Ventral margin of fore telotarsus slightly excavated and with a tiny curved seta, fore telotarsus proximally narrow and distally wide, and longer than fourth tarsomere (0.12, 0.06). Hind coxa finely punctate throughout, and dorsal outer depression absent. Inner spur of hind tibia longer than outer spur (0.24, 0.15); entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal as fourth tarsomere (0.11, 0.10). Wings ( Fig. 31L, M ). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, complete, and touching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally evenly convex, and setae evenly scattered in the margin. Metasoma ( Fig. 31A, F, I, K ). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, evenly narrowing distally and apex truncate (length 0.39, maximum with 0.22, minimum width 0.08) with pubescence on distal half. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.22, length T2 0.22), edges of median area polished and lateral grooves deep, median area longer than broad (length 0.22 mm , maximum width 0.18, minimum width 0.08); T2 scarce pubescence throughout. T3 longer than T2 (0.25, 0.22) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. White bud-like cocoon with body ridge-shaped and silk fibers evenly smooth. Cocoons scattered loose and adhered to the leaf substrate. Comments. The shape of pronotum is characteristic, the distal half is convex instead of concave. The propodeum with an incomplete median longitudinal carina. Male ( Fig. 32 A-M ). Similar in coloration to females. Males tend to be slenderer than females. Etymology. Robert (Bob) Hanner is working as Associate Director at Canadian Barcode of Life Network at the Biodiversity Institute of Ontario ( BIO ), University of Guelph , Ontario , Canada . Distribution. The parasitized caterpillars were collected in Costa Rica , ACG, Sector Pitilla ( Estacion Quica, Sendero Laguna, and Sendero Mismo), during June-July 2004 and July 2008 at 680 m in rain forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Scotura leucophleps Warren ( Notodontidae : Dioptinae ) feeding on Rinorea deflexiflora and R. sylvatica ( Violaceae ). Caterpillars were collected in third and fourth instar.