Two new phyllospheric species of Colacogloea (Colacogloeaceae, Pucciniomycotina) identified in China Author Lu, Yun-Feng https://orcid.org/0000-0003-0284-5560 School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Chai, Chun-Yue https://orcid.org/0000-0001-7753-6223 School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Hui, Feng-Li https://orcid.org/0000-0001-7928-3055 School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China fenglihui@yeah.net text MycoKeys 2024 2024-01-12 101 81 94 http://dx.doi.org/10.3897/mycokeys.101.114872 journal article http://dx.doi.org/10.3897/mycokeys.101.114872 1314-4049-101-81 13B98E3CA70C5AC6B46338C0D7F14E83 Colacogloea celtidis C.Y. Chai & F.L. Hui sp. nov. Fig. 2A Etymology. The specific epithet " celtidis " refers to Celtis , the plant genus, from which the type strain was isolated. Typus. China, Henan Province, Neixiang County, Baotianman Nature Reserve, Getiaopa ( 33°29′07″N , 111°52′51″E ), in phylloplane from leaf of Celtis bungeana , October 2022, J.Z. Li, NYUN 2210184 (holotype GDMCC 2.332T preserved as a metabolically inactive state, culture ex-type KCTC 37265 and CICC 33577). Description. On YM agar, after two weeks at 20 °C, the streak culture is cream, butyrous, and smooth. The margin is entire. In YM broth, after 7 d at 20 °C, cells are long cylindrical, 2.3-3.0 x 7.0-10.2 μm and single, budding is polar. After 1 mo at 20 °C, a ring and sediment are present. In Dalmau plate culture on corn meal agar, hyphae and pseudohyphae are not formed. Sexual structures are not observed for individual strains and strain pairs on PDA, CM agar and YCBS agar for two months. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, salicin, D-xylose (weak), D-arabinose, 5-keto-D-gluconate, ethanol (weak), glycerol, ribitol, D-mannitol, D-glucitol, succinate, D-gluconate, D-glucosamine (weak), 2-keto-D-gluconate, D-glucuronate, and glucono-1,5-lactone are assimilated as sole carbon sources. Inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside , cellobiose, L-sorbose, L-rhamnose, L-arabinose, D-ribose, methanol, erythritol, galactitol, myo-inositol, DL-lactate, and citrate are not assimilated. Nitrate, nitrite (delayed and weak), ethylamine (delayed and weak), and L-lysine (delayed) are assimilated as sole nitrogen sources. Cadaverine is not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive. Figure 2. Vegetative cells of Colacogloea celtidis sp. nov. NYUN 2210184T ( A ) and Colacogloea pararetinophila sp. nov. NYNU 2110393T ( B ) following growth in YM broth for 7 days at 20 °C. Scale bars: 10 μm . Additional strain examined. China, Henan Province, Neixiang County, Baotianman Nature Reserve, Mayigou ( 33°30′44″N , 111°55′47″E ) in phylloplane from undetermined leaf, October 2022, J.Z. Li, NYNU 221136. GenBank accession numbers. Holotype NYUN 2210184T (ITS: OP954665, D1/D2: OP954664); additional strain NYUN 221136 (ITS: OR727350, D1/D2: OR727349). Note. Phylogenetic analyses revealed that C. celtidis sp. nov. formed a single clade with high support (100 MLBP/1 BPP; Fig. 1 ). C. eltise sp. nov. can be physiologically differentiated from its closest known species C. armeniacae ( Wang et al. 2021 ) by its ability to grow in D-xylose and D-arabinose and inability to grow in trehalose and cellobiose (Table 3 ). Table 3. Physiological and biochemical characteristics that differ between the new species and closely related species.
Characteristics C. celtidis C. armeniacae * C. pararetinophila C. retinophila *
Carbon assimilation -
Trehalose - + w +
Cellobiose - + - -
Salicin + -/d/w w -
D-Xylose w - + s
D-Arabinose + - + s
D-Ribose - -/d/w + -
D-Glucuronate + + - +
Nitrogen assimilation
Nitrate + + + -
Nitrite d/w + + -
L-Lysine d - + n
Growth tests - - - -
0.1% Cycloheximide - n + -
+, positive reaction; -, negative reaction; d, delayed positive; s, slowly positive; w, weakly positive; n, data not available. All data from this study, except* which were obtained from the original description ( Sampaio 2011 ; Wang et al. 2021 ).