A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae Author Zheng, Jinshui Author Wittouck, Stijn Author Salvetti, Elisa Author Franz, Charles M. A. P. Author Harris, Hugh M. B. Author Mattarelli, Paola Author O’Toole, Paul W. Author Pot, Bruno Author Vandamme, Peter Author Walter, Jens Author Watanabe, Koichi Author Wuyts, Sander Author Felis, Giovanna E. Author Gänzle, Michael G. Author Lebeer, Sarah text International Journal of Systematic and Evolutionary Microbiology 2020 2020-04-01 70 4 2782 2858 http://dx.doi.org/10.1099/ijsem.0.004107 journal article 10.1099/ijsem.0.004107 1466-5034 DESCRIPTION OF LIGILACTOBACILLUS ARAFFINOSUS SP. NOV. Ligilactobacillus araffinosus (a.raf.fi.no’sus. Gr. pref. a not; N.L. masc. adj. raffinosus of raffinose; N.L. masc. adj. araffinosus , not fermenting raffinose). Basonym: Lactobacillus aviarius subsp. araffinosus Fujisawa et al . 1985 , 223 VP ; Fujisawa et al . 1986, (Effective publication: Fujisawa et al ., 1984, 419). Placement of L. araffinosus in a separate species is supported by ANI values and digital DNA–DNA hybridization [ 10 ]. The availability of the genome sequences of the type strains of both subspecies revealed that they are only distantly related with an ANI value of 88.98%, substantially below the 95–96 % threshold recognised for the species level. Furthermore, their core nucleotide identity (CNI) [ 10 ] is 91.1, which is below the 94 % threshold at which two strains are allocated to the same species. Finally, their in silico DDH value is 37.80, which additionally supports that these two subspecies should be recognised as different species [ 10 ]. Cells are Gram- positive, non-motile, non-spore-forming rods with rounded ends, usually occurring singly or in short chains. L. araffinosus strains ferment trehalose and cellobiose, they do not produce acid from galactose, lactose, melibiose and raffinose [ 51 ]. The genome size of the type strain is 1.48 Mbp. The mol% G+C content of DNA is 38.1. Isolated from the intestine and faeces of birds. The type strain is ML2 T = ATCC 43235 T = DSM 20653 T = CCUG 32231 T = CIP 103145 T = JCM 5667 T Genome sequence accession number: AYYZ00000000. 16S rRNA gene accession number: AB289043 .