A new species of predaceous midge in the genus Stilobezzia Kieffer from Mexico (Diptera: Ceratopogonidae) Author Huerta, Heron Laboratorio de Entomología, Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Francisco de P. Miranda No. 177, Unidad Lomas de Plateros, D. T. Álvaro Obregón, 01480, Ciudad de México. México. Author Grogan Jr, William L. 0000-0002-6475-8692 Florida State Collection of Arthropods, Florida Department of Agriculture and Consumer Services Gainesville, Florida 32614 - 7100 U. S. A. William. Grogan @ fdacs. gov; https: // orcid. org / 0000 - 0002 - 6475 - 8692 rogan@fdacs.gov text Zootaxa 2021 2021-01-14 4908 2 297 300 journal article 8832 10.11646/zootaxa.4908.2.11 bbd8e99f-37ba-4407-8166-79551b3e91b7 1175-5326 4438223 Stilobezzia (Stilobezzia) tobiasi Huerta & Grogan n. sp. ( Figs. 1–16 ) Stilobezzia (Stilobezzia) femoralis Lane & Forattini, 1956: 212 . Huerta, 2002: 57 (male, female diagnosis; in key; figs.; Mexico record); Borkent & Spinelli, 2007: 87 (in Neotropical catalog; Mexico ( Yucatan ), Panama ); Huerta & Grogan, 2017: 407 (in key to Stilobezzia of Mexico ). Diagnosis. The only Neotropical species of Stilobezzia ( S. ) with the following combination of characters. Males with dark brown thorax; fore coxa pale brown, mid and hind coxae dark brown; fore femur and tibia yellowish; mid femur dark brown on proximal 2/3, distal 1/3 yellowish, mid tibia yellowish with a narrow dark brown basal band; hind femur dark brown with yellow subapical band, hind tibia yellowish with narrow dark brown apical band; and gonocoxite with a pair of mesally directed finger-like lobes. Females with similar coloration of males; claws slightly shorter than their respective tarsomeres 5, mid claws longer than fore claws, fore claws longer than hind claws; and one ovoid spermatheca with short neck. Male . Head ( Fig. 1 ) pale yellow. Eyes narrowly separated, without interommatidial spicules. Antennal scape, pedicel ( Fig. 2 ) yellowish; flagellomeres 1–10 brown, 11–13 pale brown; flagellomeres separated, 11–13 elongated; plume dense, dark brown; antennal ratio 1.06–1.08 (1.07; n=2). Mouthparts short, light brown. Palpus pale brown; segment 3 cylindrical, with capitate sensilla on subapical mesal surface; segment 4 short; segment 5 darker, slightly shorter than segment 3; palpal ratio 4.0–4.8 (4.5; n=3). Clypeus rhomboid with 3 pairs of long, thin submarginal setae. Thorax ( Fig. 6 ). Dark brown; scutellum glossy with 2 marginal, 2 central long setae. Paratergite without seta, with lateral suture; anepisternum well developed, not bilobed posteriorly; pleura dark brown, without setae. Legs ( Fig. 6 ). Fore coxa pale brown, mid and hind coxae dark brown; fore femur, tibia yellowish; mid femur dark brown on basal two thirds (ca 0.72 of total length), extreme narrow base and apical 1/3 yellowish, tibia with a narrow faint pale brown band, remainder yellowish; hind femur dark brown, with very narrow basal and subapical yellowish band, apex dark brown, tibia yellowish with dark brown apical band; tarsi of all legs pale yellow; tarsomeres 1–2 of fore, mid legs with one row, 1–2 of hind leg with two rows of ventral palisade setae; claws small, equal-sized; hind tibial comb with 8 spines; prothoracic TR 2.09–2.45 (2.32, n=3); mesothoracic TR 2.61–3.09 (2.84, n=3); metathoracic TR 1.91–2.27 (2.06, n=3). Wing ( Fig. 11 ) membrane hyaline, with fine microtrichia; anterior veins pale brown; 1st radial cell very small, 2nd radial cell 4.2X longer than 1st; M forking at level of distal ½ of 2 nd radial cell; cubital fork at level of base of 2 nd radial cell; wing length 1.20–1.32 (1.26, n=4) mm, width 0.36–0.38 (0.37; n= 4) mm; costal ratio 0.67–0.68 (0.68; n=4). Halter dark brown. Abdomen ( Fig. 13 ). Tergites dark brown; tergite 1 posterior margin with narrow sclerotized distal band; sternites pale brown. Genitalia ( Figs. 14‒16 ). Tergite 9 longer than broad, anterior, posterior margins straight (very short, with medial cleft in 1 paratype ); sternite 9 3.5X broader than long, posterior margin slightly curved, membrane spiculate; sternite 10 spiculate, cerci short, setose. Gonocoxite stout, twice as long as broad, with a pair of finger-like lobes mesally directed. Gonostylus 0.66 length of gonocoxite, curved, base stout, with slender sharply pointed tip. Parameres large, heavily sclerotized; basal apodeme stout, laterally curved, sub-basally joined by a narrow, sclerotized band; main stems straight, becoming more widely separated posteriorly, apices tapered, bent 90º laterally, tips sharply pointed. Aedeagus composed of a pair of long, straight, heavily sclerotized bars that are connected to the base of the gonocoxite, but, they are more anteriorly situated than the parameres. Female . Similar to male with the following notable sexual differences. Antennal flagellomeres dark brown ( Fig. 3 ), except basal 2/3 of 1–8 pale yellow; antennal ratio 1.1 (n=1). Palpus ( Fig. 4 ) dark brown; palpal ratio 3.0 (n=2). Mandible ( Fig. 5 ) with 6 large, coarse medial teeth. Thorax ( Fig. 7 ). Scutellum with 6 large setae. Legs similar to male; mid tibia yellowish with narrow dark brown basal band and very narrow dark brown apical band; hind tibial comb with 7 spines; prothoracic TR 2.4 (n=2); mesothoracic TR 2.7 (n=2); metathoracic TR 2.1 (n=2); tarsomeres 5 of all legs ( Figs. 8–10 ) with a pair of sub-basal, blunt spines; claws slightly shorter than their respective tarsomeres 5, with smaller inner claw; mid claws longer than fore claws, fore claws longer than hind claws. Wing ( Fig. 12 ) membrane light brown, without brown spot; vein M 1+2 4.7X the length of r-m crossvein; 2 nd radial cell 4.5X longer than 1 st ; wing length 1.30–1.31 (1.30, n=2) mm, width 0.48–0.50 (0.49, n=2) mm; CR= 0.71 (n=2). Abdomen. Tergites shining black; tergite 1 with anterior margin not sclerotized, V-shaped, white; sternites shining dark brown. Sternite 8 not in position to describe. One ovoid spermatheca, 0.09 by 0.05 mm , with short neck. Type material. HOLOTYPE Male. MEXICO : Yucatan , Reserva Natural de Ria Lagartos : Km 10 carretera Ria Lagartos-Las Coloradas , al este de la estación Ria Lagartos , vegetacion Peten “Tucha”. GPS: [ 21° 35´46´´ N ; 88° 08´47´´W ], 8–Jul–1996 , Malaise trap , Cols. Paz-Rodriguez , R . & Huerta, H. , on slide. Colección de Artrópodos con Impor- tancia Médica ( CAIM ), Mexico City . ALLOTYPE Female : same data as holotype except, 15-Oct-1996 , on pin. PARATYPES : same data as holotype except 30-Nov-1995 , light trap , on pin, 1 female ; Tizimin , entrada a Zacbo , 4.2 km al sur de la estacion “El Cuyo”, GPS: [ 21° 28´58´´ N ; 87° 42´2´´W ], selva baja, light trap , 1-Apr-1997 , Cols. Ibáñez-Bernal, S , Manrique-Saide, P. , 1 male ; Estación Ria Lagartos, entronque sobre las carreteras Las Coloradas-San Felipe, GPS: [ 21° 34´17´´ N ; 88° 09´38´´W ], light trap , 31-Aug-1995 , Col. Huerta, H , 1 female . Reserva Natural de Ria Celestún : Rancho Loma Bonita, Km 15 carretera Celestún-Kinchil, sobre terraceria, vegetacion Peten, GPS: [ 20° 50´56´´ N , 90° 15´40´´ W ], 5-Sep-1995 , CDC trap , Cols. Navarro-Correa, C , Manrique-Saide, P. , Paz-Rodriguez, R ., 1 male ; Ecoparaiso, Km 12 carretera Celestun-Sisal, 12 km al noroeste de Celestun , duna costera, GPS: [ 20° 58´38´´ N , 90° 21´18´´ W ], Malaise trap , 17-Jul-1996 , Cols. Navarro-Correa, C. , Paz-Rodriguez, R ., 1 male ; Dumac, 1 km al este de Celestun , carretera Celestun-Kinchil, mangle, GPS: [ 20° 51´30´´ N , 90° 23´0´´ W ], 3-Jul-1997 , light trap , Cols. Manrique-Saide, P. , Paz-Rodriguez , R ., Huerta, H. 1 male . Morelos : Puente de Ixtla , San Jose Vista Hermosa , sobre la calle Josefa Ortiz de Dominguez , sitio “La Cañada”, 4-Aug to 3-Sep 2013 , Malaise trap , Col. Sergio Hernandez-Tobias , 1 male . Discussion. This new Mexican species is distinguished from other Neotropical Stilobezzia ( Stilobezzia ) by its mid femur that is dark brown on basal 2/3 and the apical 1/3 is yellowish, mid tibia yellowish with narrow dark brown basal band and very narrow dark brown apical band; hind femur dark brown with yellow subapical band and dark brown apex; and male gonocoxite with a pair of finger-like lobes mesally directed. In the similar Nearctic and Neotropical Stilobezzia ( S. ) antennalis (Coquillett) , their mid and hind femora are also black, but their fore femur and all tibiae are yellow; the male parameres are sinuate and their apices are curved inwardly, the gonocoxite with a large irregular basal lobe, the aedeagus is much shorter and broader basally; and, females have two spermathecae, but, the much smaller 2 nd spermatheca is round. In males S. (S.) femoralis Lane & Forattini (1956) , the mid femur is only brown on the basal 2/3, but the distal 1/3 is yellow; the gonocoxite is slender with only a small short, inner triangular lobe on the distal 2/3; the gonostylus is much shorter; and the wing is much longer (length 2.0 mm); however, females are unknown. In S . ( S ). wygodzinskyi Lane (1947) , the proximal 2/3 of the mid tibia and femora are dark brown, but, their distal 1/3 are yellow, however, males are unknown ( Lane & Forattini 1961 ). In S. ( S ). chaconi Macfie (1938) , the mid tibia and mid and hind femora are entirely dark brown; the gonocoxite is slender with a small short, inner triangular lobe on its distal 1/3 and it also has a sharp seta-like medial lobe. Etymology. This species is named after Sergio Hernández-Tobias for his collaboration during the collection of the specimens from Morelos and for sharing his experience with the identification of parasites of birds transmitted by species of Culicoides .