Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding Author Kirichenko, Natalia Author Triberti, Paolo Author Akulov, Evgeniy Author Ponomarenko, Margarita Author Gorokhova, Svetlana Author Sheiko, Viktor Author Ohshima, Issei Author Lopez-Vaamonde, Carlos text Zootaxa 2019 2019-08-07 4652 1 1 55 journal article 26071 10.11646/zootaxa.4652.1.1 6a9d1e6c-413c-4d86-a0ed-25794f202b06 1175-5326 3363475 6A7D6858-A43D-4FD5-8B76-FE3C1EB8DAB3 Phyllonorycter orientalis ( Kumata, 1963 ) ( Fig. 12G ) Material examined. Russia : PK , Gornotaezhnoe , forest around MTS, 43.68N , 132.15E , 224 m alt., Acer pictum , 22.VII.2016 , 1 male (reared from leaf mine), NK536 , MK 403726 ; same location, A. pictum , 23.VII.2016 , 1 larva, NK535 , MK 403729 ; same location, A. saccharum , 26.VII.2016 , 1 larva, NK538 , MK 403707 , all deposited in INRA. Leaf mine. The mine is an elongated contracted whitish blotch, without preceding epidermal tunnel, with one pronounced fold on the epidermis; situated on the lower side of the leaf nearby the leaf margin ( Fig. 12G ). Loose grains of frass are accumulated in the central part of the mine. From the upper side, the mine is white ( Fig. 12G ). Pupation in the mine. FIGURE 6. Male genitalia of Caloptilia and Phyllonorycter spp. from the Russian Far East. (A) Caloptilia gloriosa , host plant Acer pseudosieboldianum , genitalia slide [13-male]; (B) Phyllonorycter cavella , Betula dahurica , [11-2016-male]; (C) Ph. japonica , Corylus mandshurica , [4-2016-male]; (D) Ph. jozanae , Crataegus sp. [14Pj-2016-male]; (E) Ph. issikii , T. mandshurica , [NK596-male]. Sampling locations: PK, Gornotaezhnoe, (A–D) 22–25.VII.2016, (E) 21.VIII.2015. Scale bars: (A) 350, (B–E) 200 µm. FIGURE 7. Male genitalia of Phyllonorycter spp. from the Russian Far East. (A) Phyllonorycter junoniella , genitalia slide [21-male]; (B) Ph. pastorella , host plant Salix sp., [12Pp-2016-male]; (C) Ph. populifoliella , Populus balsamifera , [NK-561- 1-male]; (D) Ph. pseudojezoniella , Acer saccharum , [20Pps-2016-male]; (E) Ph. kisoensis , Alnus hirsuta , [5Pk-2016-male]. Sampling location: (A) SO, Sakhalin Isl., 14.VII.2017; (B, D) PK, Gornotaezhnoe, MTS, 22–26.VII.2016; (C) AO, Blagoveshchensk, 27.VI.2016. Scale bars 200µm. FIGURE 8. Male genitalia of Phyllonorycter and Cameraria spp. from the Russian Far East. (A) Ph. reduncata , host plant Lonicera maackii , [23Pr-2016-male]; (B) Ph. ringoniella , Malus mandshurica , [10Prin-2016-male]; (C) Ph. similis , Quercus dentata , [8Psim-2016-male]; (D) Ph. ulmifoliella [15Pu-2016-male]; (E) Cameraria niphonica , Acer pseudosieboldianum , [1Cn-2016-male]. Sampling location: Gornotaezhnoe, Primorskii Krai, 22–26.VII.2016. Scale bars: (A–D) 200, (E) 250 µm. Trophic specialization. Monophagous on Acer : A. carpinifolium , A. palmatum , A. pictum , A. pseudosieboldianum (Sapindaceae) ( De Prins & De Prins 2018 ), A. saccharum ( new record ). Distribution. Russia : RFE—PK, SO (Kuril Islands) ( Ermolaev 1977 ; Baryshnikova 2016), European part ( Baryshnikova 2008 ); Japan , Taiwan ( Kumata 1963 ), Korea ( Park & Han 1986 ).