Calls of five species of the Scinax ruber (Anura: Hylidae) clade from Brazil with comments on their taxonomy Author Magrini, Leandro Author De, Sergio P. Author Béda, Arlindo F. Author Giaretta, Ariovaldo A. text Zootaxa 2011 3066 37 51 journal article 46132 10.5281/zenodo.203149 a180146b-8bb5-4cc7-9304-842eeb857bd1 1175-5326 203149 Scinax hayii In Itapeva (MG), Scinax hayii ( 35.9–36.8 mm SVL; two males) were recorded while calling at the border of an artificial permanent pond (ca. 22º45’S , 46º12’W , 950 m asl).The advertisement call (fig. 5B; Table 1) of S . hayii from this locality consists of a single note, with 13–17 mostly fused pulses per call. The calls had a mean duration of 240±22 ms, with a mean call rate of 39.5 calls/min. The frequency spectrum ranged between 0.49–6.09 kHz. The calls showed a complex spectral structure with intra- and inter-individual variation; substantial energy was present around two bands, between 1.03–2.13 kHz, the low-frequency band (LFB) and 2.41–3.49 kHz, the high-frequency band (HFB). In most analyzed calls the LFB presented substantially more energy than the HFB (fig. 4C). In just two calls of one male both frequency bands presented almost the same relative amplitude (fig. 4D). The first pulse was generally low in intensity with calls presenting amplitude and frequency ascendant modulation from the first to the 3rd–4th pulse. In Atibaia (SP), one male of Scinax hayii was recorded at PFI (habitat and syntopic species as described for S . crospedospilus ). The calls of this male (Table 1) was similar to that from Itapeva. Calls consisted of a single note, with 13–15 mostly fused pulses per call. The calls had a mean duration of 206±11 ms, with a lesser call rate of 12.0 calls/min. The frequencies ranged between 0.48–6.39 kHz, with the most energy around two bands, 1.17–1.92 kHz (LFB) and 2.23–3.30 kHz (HFB). In all of the analyzed calls in this locality the LFB showed substantially more energy than the HFB. The first pulse and modulation characteristics as described to population from Itapeva.