A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles ninazitaniae Arias-Penna, sp. nov. Figs 175 , 176 Female. Body length 3.78 mm , antenna length 4.55 mm , fore wing length 3.33 mm . Type material. Holotype : COSTA RICA • 1♀ ; 00-SRNP-23990, DHJPAR0013364; Area de Conservacion Guanacaste , Guanacaste , Sector Santa Rosa, Bosque Humedo; dry forest; Malaise; 290 m ; 10.85145 , -85.60801 ; 07.ii.2000 ; DH Janzen & W Hallwachs leg.; ( CNC ). Paratypes . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 00-SRNP-23972, DHJPAR0013603; same data as for holotype except: 17.i.2000 ; ( CNC ). • 1 (0 ♀ , 0 ♂ ) ( 1♀ , 0 ♂ ); 00-SRNP-23975, DHJPAR0013363; same data as for holotype; ( CNC ) . Other material. Malaise-trapped material. COSTA RICA : Area de Conservacion Guanacaste , Guanacaste , Sector El Hacha , Sendero Bejuquilla : • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 99-SRNP-19251, DHJPAR0013638; dry-rain intergrade forest; Malaise ; 280 m ; 11.03004 , -85.52699 ; 05.vii.1999 ; DH Janzen & W Hallwachs leg. Area de Conservacion Guanacaste , Alajuela , Sector San Cristobal , Rio Blanco Abajo : • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 08-SRNP-3559, DHJPAR0027140; rain forest; Malaise ; 500 m ; 10.90037 , -85.37254 ; 23.iv.2008 ; DH Janzen & W Hallwachs leg. Diagnosis. Fore wing with vein 2 cu-a present as spectral vein, sometimes difficult to see, vein 1 cu-a straight, r vein slightly curved or curved, outer side of junction of r and 2RS veins not forming a stub ( Figs 175K , 176H ), dorsal groove on axillary trough of scutellum with parallel carinae ( Figs 175F , 176C ), propodeum with a median longitudinal dent ( Figs 175F , 176C ), mesoscutum proximally distinctly punctate, distally with a polished area ( Figs 175E , 176B ), temple punctate-lacunose, petiole virtually parallel-sided over most of length, but narrowing over distal 1/3, finely sculptured only laterally ( Figs 175G, H , 176D, E ), dorsal outer depression on hind coxa present ( Fig. 175A, J ), inner margin of eyes diverging slightly at antennal sockets ( Fig. 175B ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Figs 175G, H , 176D, E ). Figure 175. Glyptapanteles ninazitaniae sp. nov. female 00-SRNP-23990 DHJPAR0013364, 99-SRNP-18929 DHJPAR0012623 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T1-3, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view K, L Wings K Fore L Hind. Figure 176. Glyptapanteles ninazitaniae sp. nov. male 99-SRNP-19251 DHJPAR0013638, 08-SRNP-2976 DHJPAR0026557 A Habitus B Mesonotum, dorsal view C Scutellum, metanotum, propodeum, dorsal view D T1-3, dorsal view E, G Metasoma E Dorsal view G Lateral view F Mesosoma, lateral view H, I Wings H Fore I Hind. Coloration ( Fig. 175 A-L ). General body coloration satin black except scape and pedicel yellow-brown with lateral brown band; last four-five distal antennal flagellomeres completely yellow, remaining flagellomeres dark brown on both sides; labrum, mandible, glossa, and tegulae yellow-brown; both ends of propleuron, some small areas of metasternum, a small ventral band of mesopleuron, epicnemial ridge, distal corners of mesoscutum, lateral ends of metapleuron, and distally lunules yellow-brown/reddish; maxillary and labial palps ivory/pale yellow. Eyes and ocelli silver. Fore and middle legs dark yellow, except coxae and trochanter ivory/pale yellow, and claws brown; hind legs pale yellow except coxae proximally with an elongate black spot, femora distally with a tiny brown dot, tibiae and tarsomeres dark yellow. Petiole on T1 dark brown, contours darkened and sublateral areas ivory/pale yellow; T2 with median area black although medially reddish/brown, wide adjacent area yellow-brown, and lateral ends ivory/pale yellow with some tints yellow-brown; T3 yellow with two small brown spots medially; T4 and beyond completely brown; distally each tergum with a narrow ivory/pale yellow transparent band. In lateral view, T1-2 completely ivory/pale yellow; T3 proximal half ivory/pale yellow, distal half with some yellow-brown tints; T4 and beyond completely brown. S1-3 completely yellow; S4 yellow, but medially brown; penultimate sternum and hypopygium brown. Description. Head ( Fig. 175 A-D ). Head rhomboid with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.33:0.10, 0.32:0.10, 0.32:0.10), distal antennal flagellomere longer than penultimate (0.25:0.11, 0.19:0.11), antenna longer than body (4.55, 3.78); antennal scrobes-frons shallow. Face flat or nearly so, punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.12). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma ( Fig. 175A, E, F, I ). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct proximally with polished area distally, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS , scutellar punctation distinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS mostly overlapping the MPM ; ATS demilune with quite a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation with a smooth and shiny sloped transverse strip. Metanotum with BM wider than PFM (clearly differentiated); MPM semicircular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM ; PFM thick, smooth and with a distal flat flange; ATM proximally with a groove with some sculpturing and distally with rugae. Propodeum with a median longitudinal dent, but no trace of median longitudinal carina, proximal half curved with rather coarse sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with a mix of rugae and fine punctation, dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove deep with transverse lineate sculpture; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout. Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.41, 0.15), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Wings ( Fig. 175K, L ). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; subbasal cell with a small smooth area; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a straight, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma ( Fig. 175A, G, H, J ). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.45, maximum width 0.23, minimum width 0.12) and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.20, length T2 0.20), edges of median area polished and lateral grooves deep, median area broader than long (length 0.20, maximum width 0.24, minimum width 0.10); T2 with scattered pubescence only distally. T3 longer than T2 (0.28, 0.20) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons . Unknown. Comments. Specimen with just one fore and one hind leg although the tarsomeres are missing. Male ( Fig. 176 A-I ). The coloration of antennal flagellomeres is evenly throughout; the entire propleuron is reddish/yellow-brown with some areas darker than others; the mesopleuron with a more distinctive ventral reddish/yellow-brown band; the gena, the clypeus, the mesosternum, the ATS , the dorsal ATS groove and the lunules with reddish/yellow-brown tints; the median area on T2 is yellow-brown with contours darkened and the T3 is completely yellow. Etymology. Nina Michelle Zitani has worked in the systematics and biology of Meteorus ( Braconidae : Meteorinae ). She works at the University of Western Ontario , Canada . Distribution. The adult parasitoids were collected in Costa Rica , ACG, Sector El Hacha (Sendero Bejuquilla), Sector San Cristobal ( Rio Blanco Abajo) and Sector Santa Rosa (Bosque Humedo), during July 1999 , January-February 2000, and April 2008 at 280 m , 290 m , and 500 m in dry, dry-rain intergrade, and rain forests. Biology. Unknown. Host. Unknown.