Molecular phylogenetic analysis reveals two new species of Discosia from Italy Author Li, Wen-Jing Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road No 132, Panlong District, Kunming, Yunnan Province, 650201, PR China & Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Liu, Jian-Kui Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Bhat, D. Jayarama Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & Formerly, Department of Botany, Goa University, Goa 403206, India Author Camporesi, Erio A. M. B. Gruppo Micologico Forlivese “ Antonio Cicognani ”, Via Roma 18, Forlì, Italy Author Dai, Dong-Qing Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand & Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road No 132, Panlong District, Kunming, Yunnan Province, 650201, PR China Author Xu, Jian-Chu Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road No 132, Panlong District, Kunming, Yunnan Province, 650201, PR China Author Hyde, Kevin D. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road No 132, Panlong District, Kunming, Yunnan Province, 650201, PR China & Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand & Botany and Microbiology Department, College of Science, King Saud University, Riyadh, KSA 11442, Saudi Arabia Author Chomnunti, Putarak Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand text Phytotaxa 2015 2015-03-19 203 1 37 46 http://dx.doi.org/10.11646/phytotaxa.203.1.3 journal article 10.11646/phytotaxa.203.1.3 1179-3163 13640001 Discosia italica W.J. Li, J.K Liu & K.D. Hyde sp. nov. FIGURE 2 . Index Fungorum number: IF550758 Faces of Fungi number: FoF00330 Etymology : Named after the country from where the fungus was collected, Italy . Holotype : MFLU 14–0298 FIGURE 2. Discosia italica (MFLU 14–0298, holotype). A. Specimen. B, C. Black conidiomata on host surface. D. Vertical section of conidioma. E. Section of peridium. F–I. Conidiogenous cells and developing conidia. J–N. Conidia. Scale bars: B = 500 μm. C = 200 μm. D = 100 μm. E, F = 5 μm. G = 10 μm. H–N = 5 μm. Saprobic on dead leaves of Fagus sylvatica . Sexual state: Unknown. Asexual state : coelomycetous. Conidiomata 100–250 ( x = 180) μm diam., 20–45 ( x = 30) μm high, conspicuous, pycnidial, stromatic, amphigenous, scattered or aggregated and confluent, flattened or concave at the centre with a convex margin and a relatively thin stromatic base, rounded, black, glabrous, epidermal, unilocular or multilocular, with locules separated by cells of textura porrecta , ostiolate. Peridium 6–17 μm wide, composed of 3–4-layers, with outer 1–2-layers brown and inner 1–2-layers hyaline, composed of thin-walled cells of textura angularis . Ostiole circular, papillate. Conidiophores absent. Conidiogenous cells 2–5 × 1–3 ( x = 3.5 × 2) μm, holoblastic to phialidic, ampulliform, integrated, hyaline, smooth-walled. Conidia 10–19 × 1.5–4 μm ( x = 15 × 2.5, n = 50), cylindrical to allantoid, initially hyaline, becoming pigmented to pale brown at maturity, smooth-walled, guttulate, 3-euseptate, slightly constricted at septa, thin-walled; with basal cell obconic, slightly truncate at the base and appendaged; 2 median cells sucylindrical, with second cell from the base 4–7 μm ( x = 5.5 μm) long and third cell 2.5–4 μm ( x = 3 μm) long; apical cell subconical with a obtuse apex; apical and basal cells each with a single, simple, unbranched, filamentous appendage at the ends, apical appendage 4–10 μm ( x = 8 μm) and basal appendage 6–9 μm ( x = 8 μm). Material examined: — ITALY , Forlì-Cesena [FC], Campigna, Santa Sofia, on dead leaves of Fagus sylvatica L . ( Fagaceae ), 1 September 2012 , Erio Camporesi, IT-712 ( MFLU 14–0298 holotype ). ibid . ( KUN ! HKAS 83869, isotype ) Notes: —The fresh collections ( MFLU 14–0298) could not be cultured. Therefore, the fruiting bodies were directly used for sequencing and three sequences of D. italica were obtained and marked as D. italica A , D. italica B , D. italica C ( Figure 1 ). Similarly, two sequences of D. fagi were made from another collection ( MFLU 14–0299), viz. D. fagi A and D. fagi B .