Occurrence of Anafroptilum Kluge 2012 (Ephemeroptera: Baetidae) in Oriental Region Author Kluge, Nikita J. Author Novikova, Eugenia A. text Zootaxa 2017 4282 3 453 472 journal article 32777 10.11646/zootaxa.4282.3.2 13966ea2-f556-4d72-917e-a1b7dd85d0ec 1175-5326 827888 797FDD34-9129-4EC4-8A8F-1B7DEBA77E06 Anafroptilum odontostylus sp. n. ( Figs 54–85 ) Etymology. Referring to denticle on inner side of the first segment of male imaginal gonostylus ( Fig. 79 ) Material examined. Holotype : L-S-I ♂ {specimen [XIII] (1) 2015}, THAILAND , Mae-Hong-Son Province , river Pai upstream of Pai , 9.II.2015 , coll. N. Kluge & L. Sheyko . Paratype : the same locality, 8.II.2015 , coll. N. Kluge & L. Sheyko : L-S-I ♂ . Descriptions. Larva. Cuticular coloration. Head with light brown and colorless areas. Pronotum and mesonotum with light brown and colorless areas; protoptera light brownish with colorless stripes corresponding to longitudinal veins ( Fig. 56 ). Metanotum and thoracic pleura light brown, thoracic sterna and membranes colorless. Legs nearly colorless; apex of femur, base of tibia and outer side of tarsus diffusively tinged with light brownish. Abdomen with light brown and colorless areas identical in two examined individuals ( Fig. 54 ): terga II and VII with most extensive brown coloration, terga III and V with light median area, other terga lighter; abdominal sterna at most part colorless, with anterior margins brown. Cerci at most part light brownish, colorless at base, with posterior margin of each 4th segment slightly darkened; paracercus lighter than cerci ( Fig. 54 ). Each swimming seta of cerci and paracercus brown proximally, colorless distally. Hypodermal coloration. Unknown (probably not expressed). Structure. Frons between antennae with flat elevation; frontal suture relative long and obtuse-angled (as in Fig. 37 ). Labrum of usual shape; dorsal surface with irregularly situated setae [without constant pair of submedian setae and/or pair of latero-distal setal rows – see Anafroptilum (9)] ( Figs 60 , 76 ). Both mandibles with incisor and kinetodontium deeply separated [see Anafroptilum (10)]; planes of incisor, kinetodontium and prostheca perpendicular to plane of mandible ( Figs 61–62 ); denticles of incisors, kinetodontia and prosthecae similar to that of A. orthostylus (as in Figs 38–39 ). Molar processes of left mandible as long as distal molar projection; each process terminated by short apical lamella ( Fig. 63 : al). Maxilla with 3 canines, 3 dentisetae, 4–5 setae proximad of dentisetae and 9–10 setae of inner ventral row; all canines, dentisetae and setae slender, pointed and bent at the same direction ( Fig. 77 ) [see Anafroptilum (12)]. Maxillary palp 3-segmented, all three segments of subequal length ( Fig. 77 ). Labium with glossae and paraglossae not narrowed near bases; setae of glossa not shortened; setae of dorso-median row of paraglossa located near median margin; labial palp with 3rd segment widened apically and truncate ( Fig. 78 ) [see Anafroptilum (2)]. Vestiges of hind protoptera represented by blunt projections. Legs as in A. kazlauskasi ( Kluge 2012: Figs 38–40 ): femora nearly parallel-sided, fore femur widest at middle, middle and hind femora widest at apex; apex of each femur finely serrate ( Fig. 59 ); each femur without stout setae on outer margin and apex; only inner margin with few small stout setae [see Anafroptilum (14)]; fore tibia without patella-tibial suture, middle and hind tibia with patella-tibial suture [see Anafroptilum (1)]; each tibia with short ark of fine setae at outer side near base; outer side of tibia and tarsus without stout setae, inner side of tibia and tarsus with sparse spine-like setae. Claw with two equal regular rows of small denticles, reaching less than 1/2 of claw length ( Fig. 64 ). FIGURES 54–59. Anafroptilum odontostylus sp. n. , larva. 54, eXuviae of abdominal sterna (left) and terga (right); 55, enlarged portion of cercus; 56, eXuviae of left half of pronotum and mesonotum; 57, lateral margin of IX abdominal segment; 58, abdominal tergum X; 59, apeX of femur (54–55, 57–59, holotype). FIGURES 60–64. Anafroptilum odontostylus sp. n. , larva. 60, labrum; 61–62, left and right mandibles; 63, mola of left mandible; 64, claw (60, 61, 64, holotype). Abbreviations: al, apical lamella of molar process; dmp, distal molar projection. Abdominal terga and sterna with small denticles and sparse narrow scales ( Figs 65–68 ); sterna with irregularly situated simple long setae ( Fig. 67 ). Posterior margin of abdominal tergum I without denticles; posterior margins of terga II–VIII with blunt brown denticles ( Fig. 65 ); posterior margin of tergum XI with blunt, rounded and pointed denticles, without denticles behind pair of submedian pores ( Fig. 66 ); tergum X with pointed denticles ( Fig. 58 ). Posterior margin of abdominal sterna I–IV without denticles; posterior margin of sterna V–VIII with blunt and split denticles ( Fig. 67 ); posterior margin of sternum IX of mature male larva with prominent semicircular smooth protogonostyli and dense stick-like denticles between them ( Fig. 68 ). Paraprocts with pointed denticles on apex and median side ( Fig. 81 ). Lateral denticles either absent, or present only on distal part of abdominal segment IX ( Fig. 57 ) [see Anafroptilum (3)]. Each tergalius I–VII with long costal and anal marginal ribs, without denticles, distal margin without rib; tergalius I asymmetrically widened in distal part; tergalius II asymmetrically widened in midlength; tergalii II–VI asymmetrically widened in proximal part; tergalius VII nearly symmetrical ( Figs 69–75 ). Cerci with small denticles on all segments; one simple or composed larger spine can be present on lateral side of cercus near tip; among two examined individuals, the holotype has double spine on each cercus ( Fig. 55 ); the paratype has single spine on left cercus and vestigial spine on right cercus. Outer side of cercus without additional setae. Subimago . Cuticular coloration. Head colorless. Pronotum and prosternum light brown. Mesonotum nearly colorless with brown microtrichia, certain sutures light brown ( Fig. 83 ). Thoracic pleura and sterna at most part nearly colorless with brown microtrichia, certain sclerites light brow ( Fig. 84 ). Legs nearly colorless with brown microtrichia. Abdominal terga and sterna nearly colorless with brown microtrichia; terga with very light brownish sublateral markings. Cerci very light brownish with brown spines. Texture. On all legs all tarsomeres covered by pointed microlepides [see Anafroptilum (7)]. FIGURES 65–68. Anafroptilum odontostylus sp. n. , eXuviae of larval abdomen. 65, median part of tergum III; 66, posterior part of tergum IX; 67, median part of sternum VIII; 68, posterior part of sternum IX of male with protogonostyli. Arrows show pairs of submedian pores; abbreviations: sVIII, sIX, sterna VIII and IX; tIII, tIX, terga III and IX. FIGURES 69–77. Anafroptilum odontostylus sp. n. , larvae. 69–75, tergalii I–VII; 76, labrum and part of clypeus; 77, maXilla, dorsal view (76, holotype). Abbreviations: c1, c2, c3, maXillary canines; ds1, ds2, ds3, dentisetae. FIGURE 78. Anafroptilum odontostylus sp. n. , labium, ventral view (left) and dorsal view (right). Abbreviation: mr, median row of setae of glossa. Imago, male ( Fig. 82 ). Turbinate eyes with stem light yellow basally, grayish-white distally; facetted surface light brownish. Thorax light brownish with ocher. Legs pale ocher. In holotype , proportion of femur / tibia / tarsal segments of fore leg 70: 115: 3: 56: 42: 29: 14; middle leg 56: 56: 20: 7: 3: 12; hind leg 56: 50: 17: 6: 3: 12. Tarsus of middle and hind leg with one apical spine on 2nd tarsomere (primary 3rd tarsomere). Fore wing with membrane and veins colorless; pterostigma with 2–3 simple oblique veins. Hind wings absent. Abdomen nearly colorless, VII–X segments pale ocher. Cerci unicolor light ocher. Unistyligers and gonostyli colorless ( Figs 85 ). Unistyligers nearly meet together by their bases; styligeral muscle wide, attached to bases of unistyligers ( Fig. 79 ). Finger-like sclerotized projection between unistyligers ( Fig. 79, 80 , 85 ). 1st segment of gonostylus with prominent projection on inner side; 2nd segment arched; 3rd segment relatively large ( Figs 79 , 85 ) [see Anafroptilum (15)]. Penis brown; external part of penis wide roundishtriangular, with apical margin smooth. Female and eggs. Unknown. Dimension. Fore wing length of male 3.5 mm.