Four new and four known species of Tylencholaimoidea (Dorylaimida: Nematoda) from China Author Li, Yujuan Author Baniyamuddin, M. Author Ahmad, Wasim Author Wu, Jihua text Journal of Natural History 2008 2008-08-31 42 29 - 30 1991 2010 http://dx.doi.org/10.1080/00222930802254722 journal article 10.1080/00222930802254722 1464-5262 4651812 Dorylaimoides alpinus sp. nov. ( Figure 4 ; Table 4 ) Description Female . Body cylindrical, slightly curved ventrad upon fixation, tapering slightly towards anterior and posterior end. Cuticle finely striated 1.0–2.0 mm thick at mid body and 3–4 mm on tail. Outer cuticle narrow, with fine transverse striations; inner layer comparatively thicker than the outer one. Lateral chords occupying about one-quarter of mid body width. Lateral, dorsal and ventral body pores indistinct. Lip region offset by slight depression, about twice as wide as high or about one-quarter as wide as body width at neck base. Lips amalgamated; labial papillae not interfering with labial contour. Amphids funnel-shaped, their aperture occupying about two-thirds of lip region width. Odontostyle asymmetrical, typical of the genus, its dorsal arm longer than the ventral. Guiding ring at about 0.75–0.77 of lip region width from anterior end. Stoma cylindrical. Odontophore arcuate. Nerve ring encircling the anterior slender part of pharynx at 43–45% of neck length from anterior end. Pharyngeal expansion gradual. Expanded part of pharynx occupying about 29.5–31.5% of total neck length. Cardia short conoid, rounded, about one-quarter of corresponding body width long. Genital system amphidelphic; both the sexual branches almost equally developed. Ovaries reflexed, measuring 54–61 mm (anterior) and 62–88 mm (posterior) with oocytes arranged in a single row except near tip. Oviduct joining the ovary subterminally, measuring 67–68 mm (anterior) and 66–82 mm (posterior). Uterus measuring 81–85 mm (anterior) and 66–70 mm (posterior). Vulva transverse. Vagina extending inward about two-thirds to one-half of the corresponding body width. Pars proximalis vaginae 11– 12mm with slightly convex walls encircled by circular muscles; pars refringens vaginae absent; pars distalis vaginae 5–6 mm . Prerectum 5.3–5.8 anal body widths long. Rectum 1.4–1.6 anal body widths long. Tail short conoid, gradually tapering to a blunt terminus, ventral wall almost straight, dorsal wall convex, 1.61–1.63 anal body widths long. Two caudal pores on each side. Figure 4. Dorylaimoides alpinus sp. nov. (A) Entire female; (B) anterior region; (C) anterior end showing amphid; (D) pharyngeal region; (E) expanded part of pharynx; (F) female genital branch (anterior); (G) female posterior end. Table 4. Measurements of Dorylaimoides alpinus sp. nov. and Dorylamoides reyesi Ahmad et al., 2003 (all measurements in mm).
Characters Dorylaimoides alpines sp.nov. Dorylamoides reyesi
Holotype Paratype Females Males
female female
n 1 1 4 2
L 1552 1431 1419¡63 (1349–1516) 1682, 1657
a 45.5 44.7 36.27¡3.06 (31.5–40 43, 43.6
b 6.6 6.75 6.88¡0.34 (6.5–7.5) 5.8, 5.9
c 45.5 46 11.5¡1.86 (10–14.7) 11, 9.5
c9 1.6 1.63 5.8¡0.92 (5–6.6) 5.8, 6.1
V 40.3 40.6 39¡2.5 (36–43) –
G1 9.4 10.5 10.0¡1.46 (8.6–12.4) –
G2 6.5 10.5 12¡1.50 (10–13.5) –
Body width at neck base 31 30 32.5¡1.65 (31–35) 35, 33
Body width at mid body 34 32 39¡2.44 (36–44) 39, 38
Body width at anus 21 19 21.5¡1.5 (20–24) 26, 28
Lip region width 9 8 8.75¡0.43 (8–9) 10, 9
Lip region height 4 4 4.0¡0 5
Amphid aperture 6 5 5.33¡047 (5–6) 6
Odontostyle length 13 12 15.6¡0.47 (15–16) 15, 16
Dorsal arm of 11 10 13.5¡0.81 (12–14) 12
odontostyle
Ventral arm of 5 6 9.3¡0.47 (9–10) 9, 8
odontostyle
Odontophore length 11 11 16, 14
Guiding ring from 6 6 6.75¡0.43 (6–7) 7
anterior end
Nerve ring from 96 101 104¡3.77 (102–110) 122, 125
posterior end
Neck length 235 212 206¡5.8 (201–216) 290, 280
Expanded part of 70 67 62.75¡7.9 (54–74) 83
Pharynx
Cardia length 7 7 10.75¡1.63 (8–12) 9,6
Anterior genital branch 146 152 144¡27.3 (117–188) –
Posterior genital branch 102 153 171.5¡24.7 (145–201) –
Vaginal depth 20 16 20¡1 (19–21) –
Vulva from anterior end 625 581 555¡59 (491–653) –
Prerectum length 112 112 131¡24.7 (91–153) 183, 164
Rectum length 31 31 30¡4 (24–35) 40, 47
Tail length 34 31 125.5¡13 (103–134) 152, 172
Spicules – – – 45, 42
Lateral guiding pieces – – – 14,11
Ventromedian – – – 6, 5
supplements
Type habitat and locality Soil around the roots of trees in a mixed evergreen broad-leaved forest, Ailao Mountains and Yulong Mountains, Yunnan Province , China . Type specimens Holotype female on slide D. alpinus sp. nov. /1; paratype female on slides D. alpinus sp. nov. /2; deposited with the nematode collection of the Department of Zoology, Aligarh Muslim University, India . Diagnosis and relationships Dorylaimodes alpinus sp. nov. is characterized by having 1.4–1.5 mm long, cylindrical body; lip region offset by slight depression; 12–13 mm long odontostyle; odontophore arcuate; pharyngeal expansion gradual; amphidelphic genital system and short conoid tail. In the presence of slightly offset lip region, ampidelphic genital system, and a short conoid tail, new species resembles D. teres Thorne and Swanger, 1936 ; D. websteri Sauer, 1967 ; D. buccinator Sauer, 1967 ; D. enodis Goseco, Ferris and Ferris, 1974 and D. ornatus Peralta and Peña-Santiago, 1995 but differs from the former in having a slender body (vs a525–36), longer odontostyle (vs 6.0– 9.5 mm ), longer neck (vs 180–209 mm ), longer expanded part of pharynx (vs 51–63 mm ), comparatively longer and differently shaped tail (vs 23–30 mm long, rounded conoid). From D. websteri , the new species differs by having lip region offset by depression (vs lip region rather flat, offset by constriction); smaller amphid (vs amphid large, covering almost entire lateral area at that level); robust odontostyle (vs odontostyle slender, almost straight); pharyngeal expansion gradual (vs expanded part of pharynx offset by constriction); more anterior vulva position (vs V550–57), and shorter prerectum (vs 150–190 mm ). The new species differs from D. buccinator by having a longer, more slender body (vs L51.03– 1.32 mm , a537–40); lip region offset by depression (vs offset by constriction); longer odontostyle (vs 7 mm ); pharyngeal expansion gradual (vs expanded part of pharynx offset by constriction); more anterior vulva position (vs V547–50), and shorter prerectum (vs 140 mm ). It differs from D. enodis by having a longer and more slender body ( vs L 51.1 mm , a534.4), lip region slightly offset by depression (vs lip region amalgamated, smooth), funnel-shaped amphid (vs cup-shaped amphid), longer pharynx (vs 156.8 mm ), longer pharyngeal bulb (vs 47 mm ), comparatively lower c and c9 value (vs c534.4, c951.8), and more anterior vulva position (vs V544.5). The new species is also closely related to D. ornatus Peralta and Peña-Santiago, 1995 but distinctly differs in the shape of the tail and the absence of the peculiar irregular contour on the ventral side of tail that is so characteristic of D. ornatus .