Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species Author Fagan-Jeffries, Erinn P. C724E269-029E-49E8-8D95-6F5A5DA6BAAF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. erinn.fagan-jeffries@adelaide.edu.au Author McCLELLAND, Alana R. 3FDC78D1-CDF3-472F-B4EE-63A43C1730AF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. alana.mcclelland@adelaide.edu.au Author Bird, Andrew J. DC97FEB2-1BB0-48CE-9178-0C5F98131CC0 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. andrewbird@ozemail.com.au Author Giannotta, Madalene M. FF66BA72-4585-402F-AA42-61C9B7856048 Australian National Insect Collection, CSIRO, Black Mountain, ACT, Australia and Centre for Biodiversity Analysis, Research School of Biology, Australian National University, Acton, ACT, Australia. madalene.giannotta@gmail.com Author Bradford, Tessa M. D018F430-ED59-47BA-BF6A-EF8C6675AC20 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. tessa.bradford@samuseum.sa.gov.au Author Austin, Andrew D. DE71F924-750D-490D-84A7-F5960066F7CC Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. andrew.austin@adelaide.edu.au text European Journal of Taxonomy 2022 2022-02-08 792 1 1 116 http://dx.doi.org/10.5852/ejt.2022.792.1647 journal article 20694 10.5852/ejt.2022.792.1647 0d881922-a259-4986-99d8-8fc3919204b0 2118-9773 6037052 18DB5F54-5CEB-498E-A6F1-E570E6A57833 Glyptapanteles aspersus Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 956B5E0B-3F2A-408A-AAAE-263C197BC3DE Fig. 20 Diagnosis Glyptapanteles aspersus sp. nov. is in the G. albigena species group and can be separated from most members of the species group by the pale spot on the gena being small but clearly visible, T2 dark, the media carina completely absent and the hind femur light brown to pale. With the limited specimens available, there was not a morphological character found that easily differentiates G. aspersus sp. nov. from G. kittelae sp. nov. or G. austrinus sp. nov. The COI divergence between G. aspersus sp. nov. and G. austrinus sp. nov. is> 7%, whilst the divergence between G. aspersus sp. nov. and G. kittelae sp. nov. is also> 7%, which are considered large divergences at the species level for this group of wasps. The wingless sequences of G. aspersus sp. nov. and G. kittelae sp. nov. are very distinct, differing by 7 bp; however, there are no wingless sequences available for G. austrinus sp. nov. Etymology The species epithet ‘ aspersus ’ is an adjective, from the Latin for ‘scattered’ or ‘sprinkled’ and refers to the widely dispersed distribution of this species. Material examined Holotype AUSTRALIA • ♀ ; Western Australia , Watheroo National Park , Jingemia Caves ; -30.2542 , 115.999 ; 273 m a.s.l. ; 17 Sep.–7 Nov. 2003 ; C. Lambkin , N. Starick and J. Recsei leg.; Malaise closed heath ; Extraction684, BOLD: AUMIC450-18; WAM E109885 (previously ANIC 32 130212). Paratypes AUSTRALIA – South Australia • 1 ♀ ; Belair National Park Gate 9; -35.009 , 138.654 ; 25. Nov–1 Dec. 2007 ; J.T. Jennings leg.; Malaise trap ; Extraction59, BOLD: AUMIC374-18; WAM E109886 • 1 ♀ ; Bibaringa , Wistow ; -35.112 , 138.887 ; Jan.–Feb. 2008 ; A. Austin leg.; Malaise trap ; Extraction73, BOLD: AUMIC482-18; SAMA 32-45045 . – Victoria • 1 ♂ ; Mt Macedon ; -37.40348 , 144.57237 ; 529 m a.s.l. ; 14 Dec. 2019 ; J.B. Dorey leg.; general sweep of grass and flowering weeds in open area in schlerophyll, many gums flowering, not overly visited, sunny and overcast ~18ºC ; Extraction1153, BOLD: AUGLY064-21; SAMA 32-45046 . Description Female COLOURATION. Gena with a pale spot; labrum pale or reddish-brown; scape colour in ventral half the same colour or darker than flagellomeres; flagellomeres all black/dark brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally or uniformly reddish-brown; anteromesoscutum all dark; scutellar disk and metanotum dark; propodeum dark; fore coxa pale yellow; mid coxa pale yellow; hind coxa dark; fore femur pale yellow or orange to light brown; mid femur pale yellow or orange to light brown; hind femur orange to light brown or dark; fore tibia pale yellow or orange to light brown; mid tibia pale yellow, light brown or orange to light brown; hind tibia darkening posteriorly; hind basitarsus light brown or dark; T1 dark; T2 sclerotised area dark or dark reddish-brown, dark area extending past indentation to non-sclerotised area, but T2 lateral area then pale; T3 dark or mostly dark with paler lateral areas; T4+ dark or reddish-brown. Fig. 20. Glyptapanteles aspersus Fagan-Jeffries, Bird & Austin sp. nov. , holotype, ♀ (WAM 32 130212). A . Lateral habitus. B . Dorsal habitus. C . Fore wing. D . Anterior head. E . Dorsal head. F . Lateral head. HOLOTYPE BODY MEASUREMENTS. Body length 2.2 mm ; fore wing length 2.2 mm ; antennal length slightly shorter than body length. HEAD. Antennal flagellomere 14 length/width 2.00–2.20; antennal flagellomere 2 length/width 3.00– 4.66; OOD/POD 1.71–2.00; IOD/POD 2.00. MESOSOMA. Anteromesoscutum sculpturing with shallow punctures, space between punctures generally smaller than diameter of punctures; scutellar disk sculpturing with very shallow punctures scattered over most of area; 7–9 pits in scutellar sulcus; propodeum with median carina absent, very smooth and shiny, with either only very shallow punctures associated with setae or punctures over most of area and some shallow rugosity in posterior corners. WINGS. Pterostigma length 0.54 mm ; pterostigma width 0.22 mm ; r 0.14 mm ; 2RS 0.07 mm ; 2m 0.08 mm ; (RS+M)b 0.1 mm . METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, lateral edges straight (but not parallel); T1 smooth and shiny, some shallow scattered punctures on lateral edges; T1 length 0.38 mm ; T1 width at posterior edge 0.08 mm ; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.13 mm ; T2 width at posterior edge 0.27 mm ; ovipositor slightly protruding from end of metasoma. Male As female, but antenna slightly longer, six pits in scutellar sulcus. Remarks Glyptapanteles aspersus sp. nov. constitutes BIN: BOLD:ADL3094 and is 5.71% (p-dist.) divergent from the closet BIN in the database (BOLD:ACL9711; Glyptapanteles sp. from New Zealand ). Using the BOLD Batch ID engine, the COI barcode of the holotype is 6.6% different from the most similar COI sequence from an Australian specimen (AUMIC021-18; Glyptapanteles harveyi sp. nov. ). The holotype was able to be sequenced for the wingless gene, which differs by a minimum of 7 bp from all other species with available sequence data. Distribution This species has a wide distribution, from VIC through to SA across to western WA.