Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species Author Fagan-Jeffries, Erinn P. C724E269-029E-49E8-8D95-6F5A5DA6BAAF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. erinn.fagan-jeffries@adelaide.edu.au Author McCLELLAND, Alana R. 3FDC78D1-CDF3-472F-B4EE-63A43C1730AF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. alana.mcclelland@adelaide.edu.au Author Bird, Andrew J. DC97FEB2-1BB0-48CE-9178-0C5F98131CC0 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. andrewbird@ozemail.com.au Author Giannotta, Madalene M. FF66BA72-4585-402F-AA42-61C9B7856048 Australian National Insect Collection, CSIRO, Black Mountain, ACT, Australia and Centre for Biodiversity Analysis, Research School of Biology, Australian National University, Acton, ACT, Australia. madalene.giannotta@gmail.com Author Bradford, Tessa M. D018F430-ED59-47BA-BF6A-EF8C6675AC20 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. tessa.bradford@samuseum.sa.gov.au Author Austin, Andrew D. DE71F924-750D-490D-84A7-F5960066F7CC Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. andrew.austin@adelaide.edu.au text European Journal of Taxonomy 2022 2022-02-08 792 1 1 116 http://dx.doi.org/10.5852/ejt.2022.792.1647 journal article 20694 10.5852/ejt.2022.792.1647 0d881922-a259-4986-99d8-8fc3919204b0 2118-9773 6037052 18DB5F54-5CEB-498E-A6F1-E570E6A57833 Glyptapanteles doreyi Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 34246DE7-8966-4FBC-8D6A-ECC5DADF79BC Figs 28–29 Diagnosis Glyptapanteles doreyi sp. nov. is in the G. arcanus species group and can be separated from the other members of the species group as follows: Glyptapanteles doreyi sp. nov. can be separated from G. rodriguezae sp. nov. and G. ruhri sp. nov. by T1 being smooth and shiny, not having punctures that cover at least a third of the area of the posterior half of the tergite. Glyptapanteles doreyi sp. nov. can be separated from G. goodwinnoakes sp. nov. , G. erucadesolator sp. nov. , G. lambkinae sp. nov. , G. arcanus sp. nov. and G. vergrandiacus sp. nov. by the propodeum being less coarsely and less consistently, rugose sculptured across the anterior half of the propodeum. Glyptapanteles doreyi sp. nov. has the propodeum with shallow or strong punctures in the anterior half, the posterior half smooth or with shallow or strong rugose sculpturing and sometimes with a smooth area in the centre. Glyptapanteles doreyi sp. nov. can be separated from G. wrightae sp. nov. and G. lessardi sp. nov. by the tegula being dark in colouration, the same colour or only slightly lighter than the mesosoma (the tegula in G. wrightae sp. nov. and G. lessardi sp. nov. is pale) the hind femur mostly dark (pale or light brown in G. wrightae sp. nov. and G. lessardi sp. nov. ) and the indentation in the centre of the mesopleuron being strongly canaliculate. The indentation on the mesopleuron of G. wrightae sp. nov. and G. lessardi sp. nov. is smoother, not strongly canaliculate. Etymology Named for James Dorey, who collected the holotype specimen. EPF-J would like to acknowledge James’ collegiate sharing of specimens and his contribution to a successful field trip in northern QLD in 2019 that led to the collection of many important microgastrine specimens. Material examined Holotype AUSTRALIA • ♀ ; New South Wales , Cockburn River Camp ; -31.05222 , 151.14411 ; 461 m a.s.l. ; 10 Dec. 2019 ; J.B. Dorey leg.; 19JDEC100, general sweep over Brachychiton flowering species in schlerophyll forest along dry creek bed, sunny and warm ~33ºC ; Extraction1067, BOLD:AUGLY041-21; AM K.517929 . Paratypes AUSTRALIA – Australian Capital Territory • 1 ♀ ; CSIRO Black Mountain off Frith Rd ; -35.268 , 149.1107 ; 610 m a.s.l. ; 9–26 Oct. 2019 ; K.M. Bayless leg.; dry sclerophyll forest Malaise trap ; Extraction1660, BOLD: AUGLY125-21; ANIC 32 130318 • 1 ♀ ; same collection data as for preceding; Extraction1645, BOLD: AUGLY122-21; ANIC 32 130319 • 1 ♀ ; same collection data as for preceding; Extraction1646, BOLD: AUGLY123-21; ANIC 32 130320 • 1 ♀ (ethanol); CSIRO Black Mountain , close to Botanic Garden fence ; -35.273611 , 149.110556 ; 538 m a.s.l. ; 30 Oct.–6 Nov. 2017 ; T. Pleines and J. Rodriguez leg.; Malaise ; Extraction959, BOLD: AUGLY027-21; ANIC 32 130321 • 1 ♀ ; same collection data as for preceding; 23–30 Oct. 2017 ; Extraction974, BOLD: AUGLY028-21; ANIC 32 130322 • 1 ♀ (ethanol); CSIRO Black Mountain ; -35.2744 , 149.1115 ; 6 Dec. 2017 – 5 Jan. 2018 ; J. Rodriguez , C. Waichert , K.M. Bayless and T. Pleines leg.; Malaise 2, green dry wash ; Extraction976, BOLD: AUGLY029-21; ANIC 32 130323 • 1 ♀ (ethanol); same collection data as for preceding; Extraction978, BOLD: AUGLY030-21; ANIC 32 130324 • 1 ♀ ; same collection data as for preceding; Extraction980, BOLD: AUGLY032-21; ANIC 32 130325 • 1 ♀ (ethanol); same collection data as for preceding; Extraction981, BOLD: AUGLY033-21; ANIC 32 130326 • 1 ♀ (ethanol); same collection data as for preceding; Extraction982, BOLD: AUGLY034-21; ANIC 32 130327 • 1 ♀ (ethanol); same collection data as for preceding; Extraction983, BOLD:AUGLY035-21; ANIC 32 130328 • 1 ♀ (ethanol); same collection data as for preceding; 6–20 Jan. 2020 ; Extraction1658, BOLD: AUGLY124-21; ANIC 32 130329 • 1 ♀ ; CSIRO property ; -35.275 , 149.111 ; 588 m a.s.l. ; 7 Sep. 2011 ; P. Hebert leg.; Malaise ; BIOUG02156-H03, BOLD: MCCAA225-12; ANIC 32 130330 . – New South Wales • 1 ♀ (ethanol); same collection data as for holotype; Extraction1086, BOLD: AUGLY043-21; AM K.383784 • 1 ♀ (ethanol); same collection data as for holotype; Extraction1156, BOLD: AUGLY066-21; AM K.383785 • 1 ♂ ; same collection data as for holotype; Extraction1068, BOLD: AUGLY042-21; AM K.517930 • 1 ♂ ; same collection data as for holotype; Extraction1087, BOLD: AUGLY044-21; AM K.517931 • 1 ♂ ; same collection data as for holotype; Extraction1094, BOLD: AUGLY045-21; AM K.517932 • 1 ♂ ; same collection data as for holotype; Extraction1155, BOLD: AUGLY065-21; AM K.517933 • 1 ♂ (ethanol); same collection data as for holotype; Extraction1157, BOLD: AUGLY067-21; AM K.383786 • 1 ♀ ; Oxley Wild Rivers National Park , East Kunderang Track ; -30.818056 , 152.135 ; 7 Nov. 2015 ; D.M. Bray leg.; blue pan trap ; Extraction1248, BOLD: AUGLY073-21; AM K.517937 . – Queensland • 1 ♀ ; Lamington National Park ; -28.142 , 153.133 ; 248 m a.s.l. ; 8–18 Apr. 2007 ; C. Lambkin and N. Starick leg.; IBISCA Plot # IQ-300-D rainforest Malaise trap ; Extraction605, BOLD: AUMIC390-18; QM T208398 • 1 ♀ ; same collection data as for preceding; Extraction604, BOLD: AUMIC389-18; QM T208397 • 1 ♀ ; same collection data as for preceding; Extraction193, BOLD: AUMIC080-18; QM T208393 • 1 ♀ (ethanol); same collection data as for preceding; Extraction632, BOLD: AUMIC416-18; QM T208395 • 1 ♀ (ethanol); same collection data as for preceding; 23 Sep.–5 Oct. 2014 ; Extraction623, BOLD: AUMIC409-18; QM T208394 • 1 ♀ ; same collection data as for preceding; 5–22 Oct. 2014 ; Extraction627, BOLD: AUMIC412-18; QM T208396 . Fig. 28. Glyptapanteles doreyi Fagan-Jeffries, Bird & Austin sp. nov. , paratypes, ♀, ‘clade A’. A, F–G . AUMIC412-18. B–E . AUMIC389-18. A . Lateral habitus. B . Fore wing. C . Dorsal mesosoma. D . Anterior head. E . Lateral head. F . Dorsal head. G . Dorsal metasoma. Description Female COLOURATION. Gena without a pale spot; labrum mostly dark or reddish-brown; scape colour in ventral half uniformly paler than flagellomeres or paler than flagellomeres at proximal end; flagellomeres all black/dark brown; tegula normally dark (dark in holotype , sometimes paler in paratypes ); wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark or dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur pale yellow; mid femur pale yellow or light brown; hind femur dark reddish-brown, orange to light brown or dark; fore tibia pale yellow; mid tibia pale yellow or light brown; hind tibia darkening posteriorly; hind basitarsus light brown or dark reddish-brown; T1 dark; T2 sclerotised area dark; T2 lateral area same colour as sclerotised area, or only slightly paler or dark extends past indentation, but then pale; T3 dark, mostly dark with paler lateral areas or uniformly brown; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.4 mm ; fore wing length 2.3 mm ; antennal length slightly shorter than body length. HEAD. Antennal flagellomere 14 length/width 1.25–2.00; antennal flagellomere 2 length/width 2.00– 4.20; OOD/POD 1.57–2.50; IOD/POD 1.29–1.83. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures generally smaller than diameter of punctures; scutellar disk sculpturing with only very shallow punctures; 8–12 pits in scutellar sulcus; propodeum with median carina absent, shallow or strong punctures in anterior half, occasionally with small areas of shallow rugosity, posterior half of propodeum smooth or with shallow or strong rugose sculpturing, propodeum sometimes with smooth area in centre. Fig. 29. Glyptapanteles doreyi Fagan-Jeffries, Bird & Austin sp. nov. , paratype, ♀ (ANIC 32 130330), ‘clade B’. A . Lateral habitus. B . Fore wing. C . Dorsal head. D . Dorsal metasoma. E . Anterior head. F . Lateral head. G . Dorsal mesosoma. WINGS. Pterostigma length 0.53 mm ; pterostigma width 0.17 mm ; r 0.15 mm ; 2RS 0.14 mm ; 2m 0.09 mm ; (RS+M)b 0.08 mm . METASOMA. T1 lateral edges parallel for anterior ½ to ⅔ of length, then narrowing posteriorly; T1 sometimes shiny, smooth, or mostly smooth with some punctures in posterior half, or smooth in anterior half with indistinct sculpturing in posterior half; T1 length 0.34 mm ; T1 width at posterior edge 0.13 mm ; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.14 mm ; T2 width at posterior edge 0.31 mm ; ovipositor slightly protruding from end of metasoma. Male As female but with slightly longer antennae. Remarks Glyptapanteles doreyi sp. nov. has two wingless haplotypes (differing by a single base pair); however, as the COI divergence between specimens with the two different haplotypes is not consistent (i.e., one specimen has a COI sequence that is more closely related to specimens with the alternative wingless haplotype than to others) and the COI divergence among all specimens is not above 2%, we consider all specimens to be one species. One of the wingless haplotypes is shared with the species G. arcanus sp. nov. and G. goodwinnoakes sp. nov. and both barcodes only differ by 1 bp from the species G. vergrandiacus sp. nov. and G. lessardi sp. nov. This group of species is very closely related and would benefit from future, more detailed taxonomic work to ensure that they are not simply divergent populations of the same species. For this species hypothesis, we have made the decision to split this large clade into different species based on the COI barcode sequences being>3% divergent; however, we note that this is a hypothesis and is open to change with future work. In the IQ-TREE analysis of the concatenated COI and wingless alignment, there are two clades within G. doreyi sp. nov. that differ, in their COI sequences, between 2.0% and 2.5%. The first clade (‘clade A’) contains seven sequences (AUMIC416-18, AUMIC409-18, AUMIC080-18, AUGLY044-21, AUMIC412-18, AUMIC390-18, AUMIC389-18). Specimen AUGLY044-21 has the same wingless haplotype as the specimens in the second clade within G. doreyi sp. nov. (‘clade B’) whilst the other six specimens share a wingless haplotype that differs by one base pair from that of AUGLY044-21. We have made the conservative decision to combine these two clades into a single species due to the COI divergence being borderline of what is often used in Microgastrinae , lack of clear morphological differences and the wingless haplotype of AUGLY044-21 being identical to that of the alternate clade; however, we provide images of a representative of both clades and note that future evidence (particularly host data) may end up splitting this species further. Glyptapanteles doreyi sp. nov. constitutes BIN BOLD:ABY0421 (clade B) and BOLD:AEC8663 (clade A). Using the BOLD Batch ID engine, the COI sequence of the holotype is 2.6% different from the most similar COI sequence from an Australian specimen (MCCAA7675-20; an undescribed lineage). Distribution This species is currently known from eastern NSW and from the ACT.