Tylopilus hayatae, a new endemic bolete species in relict Mexican beech forest Author Rodríguez-Ramírez, Ernesto Chanes 0000-0001-6206-8615 Laboratorio de Biogeografía y Sistemática, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico. echanes@ciencias.unam.mx Author Martínez-González, César Ramiro 0000-0002-0256-0840 Laboratorio de Biogeografía y Sistemática, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico. ramiro_mg.unam@ciencias.unam.mx Author González-Ávila, Patricia Astrid 0000-0003-4875-4517 Laboratorio de Biogeografía y Sistemática, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico. astrid.gonzalez.avila@gmail.com Author Luna-Vega, Isolda 0000-0002-7243-9018 Laboratorio de Biogeografía y Sistemática, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico. luna.isolda@gmail.com text Phytotaxa 2020 2020-04-30 441 1 35 46 http://dx.doi.org/10.11646/phytotaxa.441.1.3 journal article 10.11646/phytotaxa.441.1.3 1179-3163 13872118 Tylopilus hayatae Rodríguez-Ramírez & Luna-Vega , sp. nov. ( Fig. 2A–D ) Index Fungorum:—IF number 556969. Etymology :—‘ hayatae’ is derived from Latin and refers to the common name of the Mexican beech ‘haya’. Holotype :— Mexico . Hidalgo state , Zacualtipán de Ángeles municipality, La Mojonera locality, 1 August 2009 , in soil under Fagus grandifolia subsp. mexicana . Rodríguez-Ramírez 180 ( M – UAEH 752 ). TABLE 1. GenBank accession numbers corresponding to the sequences used in the present study.

Species name	Voucher	Locality	GenBank accession numbers
tef1-α	rpb 1	rpb2
Tylopilus aff. ballouii	HKAS59700	China	KF112223	KF112619	KF112740
Tylopilus aff. rigens	HKAS53388	China	KF112192	KF112539	KF112688
Tylopilus argillaceus	HKAS90201	China	KT990783	-----	-----
Tylopilus	HKAS84351	China	KT990819	-----	-----
atroviolaceobrunneus Tylopilus badiceps	78206	USA	KF030429	-----	-----
Tylopilus brunneirubens	HKAS53366	China	KT990820	KT990972	-----
Tylopilus felleus	HKAS54926	Germany	HQ326866	KF112575	KF112737
Tylopilus ferrugineus	MB06053	USA	JQ327016	-----	MH614789
Tylopilus griseiolivaceus	JBSD127430	Dominican Republic	-----	-----	MN095208
Tylopilus griseipurpureus	HKAS90200	China	KT990818	KT990971	KT990458
Tylopilus hayatae	TYPE UAEH180	Mexico	MT164320	MT164326	MT164323
Tylopilus hayatae	FCME2017	Mexico	MT164321	MT164327	MT164324
Tylopilus hayatae	FCME2013	Mexico	MT164322	MT164328	MT164325
Tylopilus microsporus	HKAS59661	China	KF112225	KF112614	KF112614
Tylopilus neofelleus	HKAS50319	China	HQ326869	HQ326888	HQ326912
Tylopilus otsuensis	HKAS53401	China	KF112224	KF112613	KF112797
Tylopilus	MBO6056	China	KF112221	KF112576	KF112738
plumbeoviolaceoides Tylopilus porphyrosporus	HKAS49182	China	KT990746	KT990912	KT990383
Tylopilus rubrobrunneus	BD329	USA	-----	HQ161845	HQ161876
Tylopilus sp.	HKAS90202	China	KT990817	KT990970	KT990457
Tylopilus sp.	HKAS55438	China	KF112191	KF112538	KF112687
Tylopilus sp.	HKAS74925	China	-----	KF112577	-----
Tylopilus vinaceipallidus	HKAS90184	China	MG212613	-----	MG212656
Tylopilus violaceobrunneus	HKAS89443	China	KT990886	KT991028	KT990504
Tylopilus violatinctus	HKAS50208	China	KF112283	KF112620	KF112799

Diagnosis :—This species is distinguished from others in the genus Tylopilus by molecular sequences obtained from tef 1-α, rpb1 and rpb2 , and its velvety uniformly black pileal surface, reticulate to faintly longitudinally striate stipe with bulbose base, and yellowy white to light brownish basidiospores. Description : —Basidiome small to medium. Pileus 90–110 mm diam, plano-convex to broadly convex with incurved margin when young, expanding to sub-applante then applanate, velvety, non-hygrophanous, smooth; uniformly black, fading with age and sun exposure, developing grayish tints (1B1) especially towards the margin. Context pileus 10–15 mm thick over the stipe, white (1A1), unchanging in color, fleshy. Tubes 6–7 mm deep, depressed around stipe, light gray (4B3) when young, unchanging in color when older or when bruised. Pores 0.5–1 mm diam, mostly round, slightly radially elongated when mature at stipe, smaller near pileus margin, pale gray, concolorous with tubes. Stipe 60–80 × 10–17 mm , central, clavate to sub-clavate, with fine black reticulation to black faintly longitudinally striate, equal and curved or with bulbose base, yellowy white to light brown (5A3), changing to yellowy (1A8) when bruised, with white (1A1), basal mycelium. Taste not much and Odor mild, fruity at times with hints of cherry. Context light brown (4A2), changing to light yellow (3A4). Macrochemical reactions see Table 3 . Basidiospores (8.5–)10–15.5 × (3.8–)4–5 µm, Q= (4.9–)5–5.4(–5.5), Q m = 5.2 ± 0.23, sub-fusiform to fusiform, cylindrical to somewhat oblong, smooth, thin-walled (<1 µm), guttulate, yellowish to brownish in 10% KOH ( Figs. 2E , 3A ). Basidia (18–)20–40 × 7.5–10(–12) µm ( n = 30), clavate to sub-clavate, clamped at base, thin-walled, hyaline to yellowish in 10% KOH ( Fig. 3B ) tetrasporic, sterigmata 2–4 µm long. Cheilocystidia (18–)20–27 × 8–11(–13) µm ( n = 40), abundant, ventricose, sub-fusiform to sub-clavate, thin-walled, hyaline to yellowish in 10% KOH. Chrysocystidia 18–26(–30) × 7.5–10(–12) µm ( n = 40), similar in shape to cheilocystidia, containing yellowish pigments in 10% KOH. Pseudocystidia not seen. Pileipellis a trichodermium, of thin˗ to slightly thick-walled, generative vertical hyphae, subcylindrical to cylindrical, branched in form of an ‘H’, colorless to pale yellowish in 10% KOH, clamp connections present, clavate or nearly fusoid ( Fig. 3E ), 4–6 µm diam. Pileus trama of slightly thick-walled vertical hyphae, 4˗6 µm diam, hyaline in 10% KOH. Stipitipellis a hymeniderm composed of thin-walled (<1 µm) emerging hyphae with clavate or sub-fusiform terminal cells (4–8 µm diam), hyaline in 10% KOH. Stipe trama composed of longitudinally arranged, parallel hyphae (2–)3–7(–7.5) µm diam, cylindrical, thin-walled, hyaline in 10% KOH. Lateral stipe stratum undifferentiated from the stipe trama (‘boletoid type’), at the stipe apex a thick layer consisting of divergent, loosely intermingled and branched hyphae remaining separate and embedded in a mucilaginous substance. Clamp connections present on hyphae in all tissues. Habit, habitat and distribution:—scattered under rocky slopes areas (43.8° slope) in Mexican beech ( Fagus grandifolia subsp. mexicana ), solitary to gregarious in vitric and humic andosol soil, pH values of 4 to 6, with light sandy clay loam texture, eastern Hidalgo State , Mexico ( 20° 37’ 52’’N 96° 36’ 34’’ W , 1958 m asl). Additional specimens examined :— Mexico , Hidalgo State , Zacualtipán de Ángeles municipality, La Mojonera locality, 20° 37’ 52’’N 96° 36’ 34’’ W , 1958 m asl , 28 July 2018 , Rodríguez-Ramírez 2013 ( paratype FCME –2013) ; Mexico . Hidalgo : Zacualtipán de Ángeles municipality, La Mojonera locality, 6 August 2019 , Rodríguez-Ramírez 2017 ( paratype FCME –2017) .