Seek and ye shall find: new species and new records of Microporella (Bryozoa, Cheilostomatida) in the Mediterranean Author Martino, Emanuela Di https://orcid.org/0000-0002-3892-4036 Natural History Museum, University of Oslo - Blindern, P. O. Box 1172, Oslo 0318, Norway e.d.martino@nhm.uio.no Author Rosso, Antonietta Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Universita di Catania - Corso Italia 57, 95129, Catania, Italy & CoNISMa - Consorzio Interuniversitario per le Scienze del Mare - Piazzale Flaminio, 9, 00196, Roma, Italy text ZooKeys 2021 2021-08-02 1053 1 42 http://dx.doi.org/10.3897/zookeys.1053.65324 journal article http://dx.doi.org/10.3897/zookeys.1053.65324 1313-2970-1053-1 E67F6F86686D44AC910537FF58BC2183 24C336C5EA905902A044FCF1B43767E9 Microporella verrucosa (Peach, 1868) Figs 10 , 11 Eschara verrucosa Peach, 1868: 116. Diporula verrucosa (Peach): Hincks, 1880: 220, pl. 31, figs 1, 2; Gautier, 1962: 176; Zabala, 1986: 501, fig. 174, pl. 15A, B; Hayward and Ryland, 1979: 226, fig. 97; Hayward and Ryland, 1999: 302, figs 138C, D, 139; Andre et al. 2014 : 225, 5 figs; Rosso et al. 2014 : table 2, fig. 3A-C. Microporella (Diporula) verrucosa (Peach): Neviani, 1896a: 105; 1896b: 24. Examined material. Italy • 2 colonies and 10 fragments (living), 17 colonies and 62 fragments (dead), some very large, some regenerated and twisted; Ionian Sea, SE Sicily , Ciclopi Islands MPA; Ciclopi 2000 cruise; samples 2G, 3H, 4E, 6H, 8F, 8H, 8I, 9G, 10G, 12E, 12F, 12G, 13H, 14G; 37°32'39"- 37°34'31"N , 15°8'58"- 15°11'1"E ; 63-92.5 m ; Jul. 2000 ; A. Rosso leg.; dredging; C, DC, DE, and DL Biocoenoses; PMC. Rosso Collection I.H. B-17a. Italy • 11 living and 33 dead colonies/large fragments, 1 dead colony including the base; off Acitrezza; sample AC/L, AC/1B; coordinates unknown; 50 and 110 m ; 1980; I. Di Geronimo leg.; dredging; pre-Coralligenous and DL Biocoenoses; PMC. Rosso Collection I.H. B-17a1. Italy • 12 living and 315 dead colonies/fragments; Ionian Sea, Gulf of Noto; 36°41'45"- 36°57'47"N , 15°8'35"- 15°20'00"E ; PS/81 cruise; samples 2C, 4X, 9D (living) and samples 2B, 2C, 2XA, 2XB, 4C, 4C1, 4X, 6D, 9C, 9D, 10C, 11E (dead); Jul. 1981 ; I. Di Geronimo leg.; dredging; DC and DL Biocoenoses ; and 11 living colonies; Noto 1996 cruise; samples 8I, 10G, 10H; 77-82 m ; 1996; E. Mollica leg.; dredging; DE and DL Biocoenoses ; PMC. Rosso Collection I.H. B-17b . Italy • 18 dead colonies; Ionian Sea , Gulf of Catania ; sample LCT69; 37°18'42"N , 15°14'24"E ; 90 m ; Jul. 1980 ; I. Di Geronimo leg.; dredging; DL Biocoenosis ; PMC. Rosso Collection I.H. B-17c . Italy • 4 dead colonies; Ionian Sea , Gulf of Taranto , Amendolara Bank ; samples 1D and 5D; 39°51'42"- 39°52'54"N , 16°42'00"- 16°43'24"E ; 30-40 m ; Jun. 1991 ; R. Sanfilippo leg.; dredging; DC Biocoenosis ; PMC. Rosso Collection I.H. B- 17g . Italy • 77 dead colonies and fragments; southern Tyrrhenian Sea , SW Ustica , Apollo Bank ; 38°42'19"N , 13°7'58"E ; 60 m ; Jun. 1986 , dredging; Laminaria rodriguezii Bornet , 1888 seagrass and associated DC Biocoenosis ; PMC. Rosso Collection I.H. B-17d . Italy • 2 living colonies; Messina Strait ; coordinates unknown; 65 m ; 1990; S. Giacobbe leg.; dredging; no Biocoenosis information; PMC. Rosso Collection I.H. B- 17g . France • 50 dead colonies; Iberian-Provencal Basin , Corsica , off Calvi ; sample CL74; 42°47'31"N , 9°8'10"E ; 150- 110 m ; G. Fredj leg.; dredging; DL Biocoenosis ; PMC. Rosso Collection F.H. B-17e . Greece • 4 dead colonies, Aegean Sea , Lesvos Island , Agios Vasilios cave ; samples AV1 and AV2; 38°58'8"N , 26°32'28"E ; 30 m ; V. Gerovasileiou leg.; scuba diving; GSO and GO Biocoenoses ; PMC. Rosso Collection GR .H. B- 17f. Description. Colony erect, rigid, branched, with a limited number of relatively spaced-out bifurcations, a few cm long, raising from an encrusting basal portion (Fig. 10A ), extending up to 3 mm around the main stem. Branches cylindrical, often flattened at bifurcations (Fig. 10C, E ), consisting of 9-16 longitudinal rows of zooids, alternating very regularly to simulate an helicoidal pattern; branch diameter 10-15 mm (exceptionally up to 20 mm), becoming thicker and stouter in older portions of the colony, near the encrusting base, owing to secondary calcification. Figure 10. Microporella verrucosa (Peach, 1868) from the Ionian Sea, Ciclopi Islands MPA A, B PMC. Rosso Collection I.H. B-17a C-F PMC. Rosso Collection I.H. B-17a1 A young colony showing the encrusting portion with ancestrula (arrow) and starting developing branch (top right) B partly overgrown ancestrula and periancestrular autozooids C growing tip of a distally enlarging branch D old (proximal) branch portion showing secondary calcification obliterating orifices, sometimes only frontal avicularia are still visible E transverse section of a branch showing the thick walls converging towards the centre of the branch F close-up of wedge-shaped polypide cavities visible in transverse section. Scale bars: 500 µm ( A, B, D, E ); 1 mm ( C ); 200 µm ( F ). Encrusting base unilaminar, multiserial (Fig. 10A ), formed by autozooids similar to those of the erect branches (Fig. 10B ) but with a greater number of oral spines (5-7, commonly six) and, subordinately, autozooids with occluded orifices (not functional) and/or kenozooids, often bearing an adventitious avicularium as those associated with autozooids. Interzooidal communications via basal pore-chambers in the encrusting portion (see Rosso et al. 2014 : fig. 3A) and via multiporous septula in the erect branches (Fig. 10C ). Autozooids rounded hexagonal to lozenge-shaped, 477-779 (661 +/- 93, N = 18) x 389-615 (493 +/- 68, N = 18) μm (mean L/W = 1.34), distinct, interzooidal boundaries marked by narrow, shallow, locally undulate grooves (Fig. 11A, B, D ). Frontal shield nearly flat, finely granular, and pseudoporous; about 10 marginal areolae distinguishable from frontal pseudopores only in early ontogeny because larger, subcircular to elongate elliptical, 25-65 μm long; 19-26 subcircular pseudopores, 20-30 μm in diameter, placed centrally on the frontal (Fig. 11A, B, D ). Transverse section of the branch showing thick frontal and vertical walls, converging towards the centre of the branch, forming wedge-like polypide cavities (Fig. 10E, F ). Primary orifice approximately semi-circular to horseshoe-shaped, 130-151 (143 +/- 6, N = 10) x 145-177 (161 +/- 10, N = 10) μm (mean OL/OW = 0.89; mean ZL/OL = 4.63), outlined by a thin and smooth raised rim (Fig. 11C ); hinge-line smooth, straight to slightly concave with two short, blunt lateral condyles and a smooth, proximal shelf sloping outwards; distal margin of the orifice corrugated because of a deeply placed, drawstring-like, arched rim seemingly functioning as support for the closed operculum. Oral spines four, occasionally five, thin (base diameter 15-20 μm ), relatively short (60-90 µm ), placed distally, often detached (Fig. 11A, B, D ). Ascopore field a narrow, reniform to U-shaped rim of smooth gymnocystal calcification, 50-60 x 57-80 µm , placed 65-80 μm below the orifice, same level as the orifice and the adjacent frontal shield; ascopore opening transversely C-shaped, 40-63 x 5-12 μm , with a massive, upside-down mushroom-shaped tongue projecting from distal edge with radial spines (Fig. 11A, B ). A single, constant, large avicularium, 121-156 (142 +/- 9, N = 20) x 119-139 (130 +/- 7, N = 20) μm (mean AvL/AvW = 1.09), located laterally, on either side, at about half zooidal length (Figs 10B, C , 11A, B, D, F ); crossbar complete; rostrum short, rounded triangular, channelled, directed laterally or less often distolaterally and slightly upward. Mandible 142-273 μm long, with a pointed, hooked tip, toothed at the level of the rostrum tip, lining proximally to the ascopore when open (Fig. 11F ). Figure 11. Microporella verrucosa (Peach, 1868) from the Ionian Sea, Ciclopi Islands MPA, PMC. Rosso Collection I.H. B-17a A typical rhomboidal autozooids with large avicularia, transversely C-shaped fissure-like ascopore at the same level as the frontal shield, large pseudopores and larger marginal areolae, and thin oral spines surrounding the distal half of the orifice B autozooids, two of which have ovicells pierced by small, evenly distributed pseudopores, and outlined by a row of few, large, elongate pores C horseshoe-shaped orifice with blunt lateral condyles and slightly corrugated proximal border at the end of a smooth proximal shelf. Note the distal rim of calcification D a possibly abutted autozooid lacking the orifice E autozooids, one exceptionally developing paired avicularia F unbleached colony portion with opercula and open mandibles. Scale bars: 200 µm ( A, B, D-F ); 50 µm ( C ). Ovicell non-personate, subglobular, prominent, large, 250-327 (286 +/- 36, N = 4) x 384-430 (402 +/- 20, N = 4) μm (mean OvL/OvW = 0.71), formed by the distal autozooid, obscuring half of the zooidal orifice; calcification fabric similar to frontal shield but with larger and more prominent tubercles, and smaller (15-20 μm in diameter), more closely spaced pseudopores, seemingly radially aligned with rows separated by raised ridges; a discontinuous, peripheral row of larger pseudopores sometimes present (Figs 10C , 11B, E ). Ancestrula tatiform (Fig. 10A, B ), oval (220 x 150 μm ), gymnocyst concealed, cryptocyst smooth, narrowing distally; opesia oval (160 x 110 μm ); only four, distal spines visible on the single ancestrula observed. Ancestrula budding two distolateral autozooids, and subsequently surrounded by two lateral and one proximal autozooids. Older colony parts thickly calcified owing to secondary calcification progressively obliterating zooidal openings including orifices, ascopores and avicularia (Fig. 10D ), making difficult the distinction between old autozooids and genuine kenozooids that probably also develop. Remarks. First assigned to Eschara ( Peach 1868 ), Eschara verrucosa served as the type species of the genus Diporula Hincks, 1879 in which it has been included since then with the exception of Neviani (1896a , b ). Characters used to distinguish Diporula from Microporella included the "dendroid zoarium with cylindrical branches" and the morphology of the orifice described as "expanded above, contracted below, and slightly constricted by lateral projections (horseshoe-shaped)" ( Hincks 1880 : 220; and similar description in Gautier 1962 : 176). However, both characters seem feeble to justify the distinction between the two genera. At least nine species of Microporella possess erect colony-growth, starting with a more or less developed encrusting portion as does Diporula . Also the shape of the orifice in Microporella species is highly variable ( Di Martino et al. 2020a ), with several examples of species having orifices with the proximal margins narrower than the orifice maximum width [e.g., Microporella curta Almeida, Souza, Mengola & Vieira, 2017 from Brazil, Microporella franklini (Soule, Chaney & Morris, 2003) from California, the Mediterranean Microporella genisii (Audouin & Savigny, 1826), Microporella hastingsae Harmelin, Ostrovsky, Caceres-Chamizo & Sanner, 2011 from the Red Sea, and the Arctic Microporella klugei Kuklinski & Taylor, 2008]. Further differences between Microporella and Diporula were highlighted by Hayward and Ryland (1999 : 292, 312), including interzooidal communications via basal pore-chambers in the former genus and multiporous septula in the latter, and the presence of pseudopores in the ovicells of Diporula . However, multiporous septula were observed, for example, in Microporella ordo (see Di Martino et al. 2020a : fig. 7D), and basal pore-chambers were observed in the encrusting portions of M. verrucosa , while pseudopores occur in the ooecium of many Microporella species including the type M. ciliata (see Kuklinski and Taylor 2008 : fig. 1c). A further presumed difference relates to the ooecium porosity, with Diporula reported as having a fully perforated endooecium and Microporella species usually described as having only pits in the endooecium ( Harmelin et al. 2011 : 2; Ostrovsky 2013 : figs 2.43B-D, 2.44A). However, pores clearly perforate the endooecium also in Microporella as seen in broken ooecia of M. ichnusae sp. nov. (Fig. 6B ) Based on these observations, here we propose Diporula as junior synonym of Microporella and resurrect the combination Microporella verrucosa first proposed by Neviani (1896a , b ). Specimens of a second species of Diporula , D. coronula Ortmann, 1890 need re-examination. Based on the original description and illustration ( Ortmann 1890 : 39, pl. 3, fig. 7), this species has a lepralioid orifice with condyles at about one-third of the orifice length, a single avicularium with spathulate mandible, and up to two frontal foramina, characters reminiscent of other cheilostome genera such as, for example, Poricella Canu, 1904. Specimens originally described as Eschara lunaris Waters, 1878, from Pleistocene sediment of eastern Sicily and synonymised with M. verrucosa by Hincks (1880) need to be re-examined as well to confirm their conspecificity, but this is out of the scope of the present paper. The rugose appearance observed by Peach (1868) and Hincks (1880) , which inspired the species name, was not observed in our material, although secondary calcification is always very common in older parts of the colony. Intramural budding is rare and restricted to avicularia, while branch regeneration is common, apparently following breakage as indicated by broken autozooids with sharp edges. We also observed zooids with reverse polarity, sometimes budded from old stems with autozooids obliterated by secondary calcification. However, in these instances few whorls of autozooids usually develop from the regeneration surface, with only few tips appearing actively growing. Colony fragments longer than 2 cm can appear twisted, a morphology observed in some cyclostomes ( Harmelin 1976 ) and other erect cheilostomes from the Gulf of Noto and the Ciclopi MPA area ( Rosso 1989 ). This twisted branch morphology and the ability to regenerate after breakage might represent the adaptation of this species to colonize soft sediment bottoms. Basal, encrusting colony portions are relatively common in our samples and show that the ancestrulae settled on clasts ranging from a few mm to 1-2 cm in size. The ability of this species to encrust small particles, in addition to large substrates in rocky habitats, was suggested by Gautier (1962) after finding only colony fragments in dredges from sandy-muddy bottoms. The diagnostic characters of this species seem constant in the Mediterranean specimens, except for the size of the ascopore related to the development of the distal tongue sometimes leaving only a fissure-like opening. Paired avicularia were observed only in one autozooid (Fig. 11E ). Higher variability is observed when comparing the Mediterranean specimens with those from the Atlantic (e.g., Hayward and Ryland 1999 : 302, figs 138C, D, 139A, B; unpublished SEM images provided by P.D. Taylor from Mauritania and Madeira) related to the ascopore shape, the size of the spines, and the distribution and size of pseudopores on the frontal shield, suggesting the existence of a species complex. Distribution and ecology. Microporella verrucosa is a warm-temperate species with Atlanto-Mediterranean distribution. In the Atlantic, it has been reported from West Africa to the southwest of the British Isles ( Hayward and Ryland 1999 ); in the Mediterranean, it occurs preferentially in mid- and outer-shelf habitats below 50-60 m depth, with an optimum at 70-120 m ( Gautier 1962 ; Zabala 1986 ; Rosso 1989 , 1996a ; Rosso and Di Martino 2016 ), but it was also observed at shallower depths (20 m) in a shadowed open cave in Catalonia ( Andre et al. 2014 ). It is associated with shadowed rocky habitats, including the Coralligenous and Semi-Dark and Dark Cave Biocoenoses, and detritic habitats, such as the Coastal Detritic and the Offshore Detritic Biocoenoses (Table 1 ; Gautier 1962 ; Harmelin 1969 , 1976 ; Zabala 1986 ; Rosso 1989 , 1996a , 1996b ; Di Geronimo et al. 1990 ; Madurell et al. 2013 ; Rosso et al. 2014 , 2019b ; Gerovasileiou and Rosso 2016 ). However, it is never very common or dominant at sample or habitat scale, occurring only with a few colonies per sample and/or in one out of four or five sampling stations (Table 1 ; see also Harmelin 1976 : tables 1, 3). Table 1. Microporella species studied in this paper with related sampling information and number of specimens found in each sample; numbers in brackets refer to dead specimens. Abbreviations for samples: PS/81, Piattaforma Siciliana cruise; N/96, Noto cruise; MZ and GR, Mazzere and Granchi caves from Plemmirio MPA; Cast. beach., Castelluccio beached; LCT, Gulf of Catania cruise; CI, Ciclopi 2000 cruise; AC and SM, Ciclopi Islands MPA samples; AM, Amendolara Bank cruise on board of Urania; PC, Porto Cesareo; Ta I, Taccio I; Ma, Madonna; Sc, Scoglio di Fora; ECE and EBE, Marettimo; CL, Calvi cruise of the University of Nice; AV, Agios Vasilios; c., cave; cn., Canyon; Isl., Island. Abbreviations for Biocoenoses: DC, Coastal Detritic Bottoms; DL, Offshore Detritic Bottoms; C, Coralligenous; VTC, Terrigenous Muddy Bottoms; DE, Muddy Detritic Bottoms; GSO, Semi-Obscure caves; IA, Infralittoral Algae ; HP, Posidonia Meadow; GO, Obscure Caves; CB, Cold-Water Corals; na, not applicable; f, fragment.
Sea/ Locality Sample Depth Biocoenosis Microporella appendiculata Microporella bicollaris sp. nov. Microporella ciliata Microporella ichnusae sp. nov. Microporella modesta Microporella pachyspina sp. nov. sp. A Microporella verrucosa
Ionian/ Gulf of Noto PS/81 CR1 45 DC 27(8) - - - 16(12) - - -
PS/81 2B 65 DC - - - - - - - (2)
PS/81 2C 83-74 DC - - - - - - - 1(44)
PS/81 2XA 128 DL - - - - - - - (4)
PS/81 2XB 120 DL - - - - (1) - - (28)
PS/81 4C 95-86 DL - - - - (1) - - (32)
PS/81 4C1 89-84 DL - - - - - - - (44)
PS/81 4X 102-93 DL - - - - (1) - - 1(33)
PS/81 6D 98-96 DL - - - - - - - (87)
PS/81 9B 44 DC (1) - - - (1) - - -
PS/81 9C 60 DC - - - - (1) - - (3)
PS/81 9D 78 DC - - - - (12) - - 10(24)
PS/81 10C 60 DC (1) - - - (1) - - (1)
PS/81 11E 98 DL - - - - (2) - - (13)
N/96 3C 20 C - - - - 11 - - -
N/96 5E 40 C-DC - - - - 1 - - -
N/96 6C 45 VTC 2 - - - - - - -
N/96 7E 35 C - - - - 12 - - -
N/96 8I 77 DE - - - - - - - 1
N/96 9E 50 DC 1 - - - - - - -
N/96 10G 82 DE - - - - 1 - - 6
N/96 10H 80 DE-DL - - - - - - - 4
N/96 10 I 107 DL - - - - (1) - - -
N/96 WP 90 DL - - (1) - (24) - - -
Ionian/ Plemmirio caves MZ1 ≈23 GSO - (1) - - - - - -
GR E ≈19 C - - - - 3(1) - - -
Ionian/ Gulf of Catania Cast. beac. 0 na - - - - 15 - - -
LCT69 90 DL - - - - (2) - - (18)
Ognina 4 plastic - - - - 2 - - -
CI 2G 87.5 DE-DL - - - - (4) - - 1(3)
CI 3H 71 DC - - - - - - - (1)
CI 4E 52 DC 1 - - - - - - (1)
CI 6H 75 DC? - - - - - - - 1(2)
CI 8F 79 DC - - - - - - - (1)
CI 8H 92.5 DE-DL - - - - - - - (1)
CI 8I 95 DE-DL - - - - (3) - - (1)
CI 9G 63 DC - - - - (10) - - (30)f
CI 10G 85 DC-DE - - - - - - - (1)
CI 12E 62 DC - - - - (7) - - (2)
CI 12F 70 DC - - - - (1) - - (2)
CI 12G 83 DE-DL - - - - (5) - - (1)
CI 13H 105 DL - - - - - - - 10(32) f
CI 14G 90 DL 1 - - - - - - (1)
AC/L 50 C - - - - - - - (16)
AC/1B 110 DL - - - - - - - 11(18) f
SM1Z25 25 IA - - - - 1 - - -
Ionian Messina Strait 65 no data - - - - - - - 2
Ionian/ Gulf of Taranto AM 1D 30-40 DC - - - - 1 - - (3)
AM 5D 40 DC - - - - 1 - - (1)
PCI 10 5-15 C - 2(1) - - - - - -
Sicily Strait/ Pelagian Island Ta I cave 10-20 C-GSO - - - - 1(1) - - -
Ma cave 15 GSO - - - - 1 - - -
Sc cave 10 GSO - - - - 2(2) - - -
Sicily Strait/ Egadi Island ECE 5 8 IA; IA-HP - - - - ca.100 3 - -
EBE/EBI 19 - - - - - 15 - -
Tyrrhenian Palinuro c. 46 GO - - - - - - (1) -
Ustica Isl. 60 C - - - - 6(2) - - (77)
Iberian-Provencal basin/Sardinia, Capo Caccia, and Asinara Falco 1 7 GSO 2 - - - - - - -
Falco 2 4 - - - 3 - - - -
Bisbe 1 8 2 - - 1 - - - -
Bisbe 2 8 1 - - 4 - - - -
Galatea 1 8 - - - 1 - - - -
Galatea 2 6 - - - 1 - - - -
PSE/PSI 5-15 IA - - - - 7 - - -
W Corsica CL 74 150-110 DL (11) - - - - - - (50)
Adriatic Bari cn. 1B1 280 CB - - - - (1) - - -
Aegean/ Lesvos Island AV1 30 GSO (1) - - - - - - (1)
AV2 30 GO - - - - - - - (3)