Twenty-one new species of the Simulium (Gomphostilbia) asakoae species group (Diptera, Simuliidae) in Thailand, with their genetic relationships Author Takaoka, Hiroyuki Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, 50603, Kuala Lumpur, Malaysia Author Srisuka, Wichai Entomology Section, Queen Sirikit Botanic Garden, P. O. Box 7, Maerim, Chiang Mai 50180, Thailand Author Fukuda, Masako Institute for Research Promotion, Oita University, Idaigaoka 1 - 1, Hasama, Yufu City, Oita, 879 - 5593, Japan Author Saeung, Atiporn Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand https://orcid.org/0000-0003-3550-5992 atisaeung.noi@gmail.com text ZooKeys 2020 950 51 152 http://dx.doi.org/10.3897/zookeys.950.51298 journal article http://dx.doi.org/10.3897/zookeys.950.51298 1313-2970-950-51 3E805885D3354FB3AE8DFA443FAD82AE 7F08092C01585A70A7948EA57A2A8E2E Simulium (Gomphostilbia) asakoae Takaoka & Davies, 1995 Simulium (Gomphostilbia) asakoae Takaoka and Davies 1995 : 55-60 (female, male pupa, and larva). Specimens used for DNA analysis. One female, reared from a pupa collected from a stream (width 30 cm, depth 5 cm, bed sandy, moderate flow, pH 6.3, 23.6 °C, exposed to the sun, elevation 1,097 m, 18°50'03.7"N , 99°22'32.2"E ), at Pa Miang Village, Chae Hom, Lampang Province, Thailand, 9-VIII-2016, by W. Srisuka (Coll. No. 86); one male, reared from a pupa collected from a stream (width 5 m, depth 25 cm, pH 6.9, 22.1 °C, moderate flow, bed sandy, partially shade, elevation 759 m, 16°19'01.2"N , 99°06'19.9"E ) near Tao Dam Waterfall, Klong Lan, Kam Phaeng Phet Province, Thailand, 20-XII-2016, by W. Srisuka (Coll. No. 145); one female and one male, reared from pupae collected from a stream (width 2.5 m, depth 30 cm, moderate flow, bed sandy, partially shaded, pH 5.9, 19 °C, elevation 1,293 m, 16°58'49.5"N , 101°03'29.3"E ) at Rangkla Village, Nakhon Thai District, Phitsanulok Province, Thailand, 18-VI-2019, by W. Srisuka (Coll. No. 200); one female reared from a pupa and one mature larva collected from a stream (width 50 cm, depth 16 cm, moderate flow, bed sandy, pH 7.3, 21 °C, elevation 1,231 m, 18°32'16.3"N , 98°31'30.5"E ) at Khun Klang Village, Doi Inthanon National Park, Chiang Mai Province, northern Thailand, 24-VII-2019, by W. Srisuka (Coll. No. 201).