Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov. Author Bubner, Ben Author Buchheit, Ramona Author Friedrich, Frank Author Kummer, Volker Author Scholler, Markus text MycoKeys 2019 48 1 40 http://dx.doi.org/10.3897/mycokeys.48.30350 journal article http://dx.doi.org/10.3897/mycokeys.48.30350 1314-4049--1 Milesina spp. Abies alba Mill. Austria, Steiermark: Trog, Mausegg, Hoellental , Klamm, N river bank, 31 Jul 2018, M. Scholler & C. Scheuer, 0, I (KR, KR-M-0050303); Germany, Baden-Wuerttemberg : Esslingen, Kirchheim unter Teck, NW Owen, 18 Sep 2017, R. Buchheit, I (KR, KR-M-0049065); Freudenstadt, Baiersbronn, NSG "Wilder See-Hornisgrinde", Karwand, mixed spruce-fir forest, 12 Sep 2015, M. Scholler, I (KR, KR-M-0043687); Ortenaukreis, Friesenheim, E Oberweier near Schnaiggraben, mixed forest, 17 Aug 2005, M. Scholler (KR, KR-M-0018587); Ortenaukreis, SSW Oppenau, "Ibacher Holzplatz" mixed spruce-fir forest, 18 Aug 2005, M. Scholler (KR, KR-M-0018624); Ottenhoefen , "Edelfrauengrab Wasserfaelle" , creek bank, 26 Jun 2017, M. Scholler (KR, KR-M-0048773); Rastatt, Gernsbach, Lauterbach, NSG "Lautenfelsen" , eastern crosscut, beyond "Aussichtfelsen" , 11 Sep 2014, M. Scholler (KR, KR-M-0042052); Rastatt, Loffenau, "Grosses Loch" near Loffenau, 15 Sep 2017, R. Buchheit (KR, KR-M-0049038); Reutlingen, W Bad Urach, "Tannenverjuengung" , 18 Sep 2017, R. Buchheit, I (KR, KR-M-0049068); Bayern: Garmisch-Partenkirchen, Grainau, S Eibsee, spruce-fir forest, 8 Aug 2017, M. Scholler (KR, KR-M-0049063); Miesbach, Schliersee, S Spitzingsee, E Rosskopf , spruce-fir forest close to Struthiopteris spicant , 8 Aug 2017, M. Scholler (KR, KR-M-0049062). Comment. In this study, Milesina spp. on Abies alba were only sequenced but not morphologically analysed. Subgeneric classification Four morphological groups can be distinguished within Milesina with respect to germ pore number, germ pore size, germ pore position and distribution of spines on the spore surface (Figures 6 a-r , 7 d-f , Table. 4). The morphological differentiation corresponds with the differentiation in four clades found by molecular data (Figure. 1, right panel).