Crenobiont, stygophile and stygobiont molluscs in the hydrographic area of the Trebisnjica River Basin Author Falniowski, Andrzej https://orcid.org/0000-0002-3899-6857 Department of Malacology, Institute of Zoology and Biomedical Research, Jagiellonian University, ul. Gronostajowa 9, 30 - 387 Krakow, Poland Author Lewarne, Brian The Devon Karst Research Society, Library & Office, 46, Morley Court, Western Approach, Plymouth, Devon, UK Author Rysiewska, Aleksandra Department of Malacology, Institute of Zoology and Biomedical Research, Jagiellonian University, ul. Gronostajowa 9, 30 - 387 Krakow, Poland Author Osikowski, Artur https://orcid.org/0000-0001-6646-2687 Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24 / 28, 30 - 059 Krakow, Poland a.osikowski@urk.edu.pl Author Hofman, Sebastian https://orcid.org/0000-0001-6044-3055 Department of Comparative Anatomy, Institute of Zoology and Biomedical Research, Jagiellonian University, ul. Gronostajowa 9, 30 - 387 Krakow, Poland text ZooKeys 2021 2021-06-28 1047 61 89 http://dx.doi.org/10.3897/zookeys.1047.64034 journal article http://dx.doi.org/10.3897/zookeys.1047.64034 1313-2970-1047-61 835A7E2B82E845B09E3FB8548031A048 23C3761CE1BF5BFAA5301C213FB1D457 Ancylus sp. Fig. 14L, M GenBank no. COI: MW879254-MW879255 Remarks. Considering the shell morphology, it should be determined as A. fluviatilis O. F. Mueller , 1774, a species reported from this region. However, Pfenninger et al. (2003) demonstrated that A. fluviatilis inhabits a wide range throughout Europe, but in the southern regions there are a few cryptic, molecularly defined species of Ancylus . Our Ancylus sp. molecularly belonged to the Clade 4 of Pfenninger et al. (2003) and " Ancylus sp. C4" of Albrecht et al. (2006) (Fig. 15 ). It was found as a crenobiont in the cave springs at the localities 8, 9 and 16 (Fig. 4 ). Molecular divergence between this Ancylus sp. and Ancylus recurvus is 7%, and similar value (7.5%) is observed between this Ancylus sp. and A. fluviatilis . Figure 15. Molecular relationships of the studied Ancylus based on COI; our sequences in red and orange, the other from GenBank; bootstrap supports given if over 60%, their values together with Bayesian probabilities.