Four new species of Sabatieria Rouville, 1903 (Nematoda, Comesomatidae) from the Continental Slope of Atlantic Southeast Author Botelho, Alessandra Prates Author Silva, Maria Cristina Da Author Esteves, André Morgado Author Fonsêca-Genevois, Verônica text Zootaxa 2007 1402 39 57 journal article 10.5281/zenodo.175447 09b40e82-de5d-45a4-96dd-133360ca1481 1175­5326 175447 Sabatieria spiculata sp.nov. (figure 2 and 3) Description Studied material: 6 male ; 4 female Type material: Male hollotype MNRJ 305; female allotype MNRJ 306; 5 males 46–50 NM LMZOO­ UFPE; Three females 51–53 NM LMZOOUFPE. Type locality: Campos Basin (Rio de Janeiro, Pernambuco, Brazil ). It was found at 750 and 1650 meters depth, in silt­clay sediments. Measurements: see table 2. Etymology: The specific name spiculata refers to the long and sligth spicule. Male: Cylindrical body, medium­sized, tail short with cylindro­conical shape. Punctated cuticle with transversal rows of dots and lateral differentiation with larger and wider spaced punctations, specially in the tail region. Rounded and attenuated head with anterior sensilla arranged in three crowns: six minute internal labial sensilla, six external labial setae and four cephalic setae. Somatic setae, short.. Amphidial fovea spiral, with three turns. Buccal cavity cup­shaped, with diminute dorsal teeth. Pharynx clavate, gradually expanding towards to the posterior end, but without distinct bulb. Cardia short, pear shaped. Nerve ring slender located at the middle of pharynx and anterior to secretory­excretory pore. Ventral gland opposite posterior end of pharynx and beginning of intestine; system diorchic; opposed and outstretched testes. Anterior testis situated on the left of the intestine and the posterior one on the right side. Spicule paired with the same length equally wide and slightly curved ventrally; proximal half with mid­septum (each spicule has inside the core sensilla). Several glandular cells are arranged around the spicules. Small gubernaculum with narrow apophysis. Minute porelike precolacal supplements and precloacal setae. Spermatozoa oval, elongated, showing some striation. Conical tail with three caudal glands, ending in a spinneret. Two caudal setae. TABLE 2. Measurements (µm) of Sabatieria spiculata sp.nov.

HOLOTYPE	PARATYPE	ALLOTYPE	PARATYPE
Male	Males= 5	Female	Females= 3
At	3	3	3	3
L	1860	1505–1735	1855	1570–1830
hd	15	16–17	20	15–19
buc cav	7	6–9	7	6–9
Cs	2,5	2,5–3,7	3	2,5–3,8
amph dist	8	6–11	8	6–9
amph wid	13	12–13	12	10–11
amph cbd	17	17–21	20	16–20
ex pore	114	106–111	132	114–124
nerring	109	99–106	108	105
ph	216	192–211	223	204–222
bulb cbd	46	42–51	52	44–50
bulb d	29	27–30	33	30–35
M	48	40–56	68	42–49
v	–	–	832	755–909
V %	–	–	45	48–53
v cbd	–	–	68	42–49
spic	53	55–62	–	–
abd	47	37–45	48	29–43
apofise	15	19–25	–	–
sp	10	11–14	–	–
tail	94	70–94	90	78–88
s term	2,5	2,5–4	3	3–4
som s	3	4	3	3
a	38,8	30,8–40,2	27,3	27,3–38,2
b	8,6	7,4–8,3	8,3	7,7–9
c	19,9	17,9–21,4	20,6	19,5–22,4
A%	76,5	60,7–74,9	60,0	53–60,8
c'	2,0	1,9–2,4	1,9	1,9–2,8

Female: Females are quite similar to males, although some minor morphological differences are observed. Total body length and head diameter similar to males. Amphidial fovea spiral with three turns. Buccal cavity cupshaped with minute dorsal tooth. Pharynx clavate. Nerve ring situated at the middle of pharynx. Secretoryexcretory pore somewhat more posterior than in male. Reproductive system didelphic­amphidelphic, with outstretched ovaries. Egg proximal to vagina in the anterior part of uterus. Vulva at mid­body. Vagina thinwalled; pars proximalis vaginae (= vagina uterina) surrounded by a constrictor muscle. Dilators vaginae extending from the uterus wall opposite the vagina towards the subdorsal body wall. Finely granular vaginal glands present at the pars distalis vaginae (figure 2C). Tail conical with three caudal glands ending in a spinneret. Posterior end of tail with two short somatic setae. FIGURE 2. Sabatieria spiculata sp.nov. A. Total view of male; B. Pharyngeal region of male, C. Vulva region; D. Copulatory apparatus and tail region in oblique view; E. Posterior body region showing the arrangement of precloacal supplements; F. Female reproductive system. Diagnosis S. spiculata sp.nov. is characterised by a minute dorsal tooth; 10–14 pore­like precloacal supplements; narrow spicules with 53–62µm; tail conical (70–94 µm). FIGURE 3. Sabatieria spiculata sp.nov. A. male; B. Anterior end; C. Female; D. Precloacal supplements of male; E. Amphid of male; F. Buccal cavity of male (arrow indicate tooth); G. Tail and spicules; H. Lateral punctations on male tail; I. Lateral punctations on male at the level of pharynx posterior end; J. Vulva region. Relationships S. spiculata sp.nov. resembles S.conicauda Vitiello 1970 and S. dorylaimopsoides Allgén 1959 in the shape of head, tail, spicule and supplements. Can be distinguished from S.conicauda by the total lenght (L= 1051–1153 ɗ and 1153 Ψ), number of supplements (7–8) and non armed buccal cavity. Difers from S.dorylaimopsoides by the thorn in buccal cavity and the reproductive system in female with reflexed ovaries.