Multi-locus molecular phylogenetic analysis reveals two new species of Amphichorda (Bionectriaceae, Hypocreales) Author Wang, Zhi-Qin 0000-0001-9022-3635 Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China Author Zhao, Jing Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China Author Dong, Quan-Ying Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China Author Wang, Yao Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China Author Lu, Ying-Ling Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China Author Luo, Run Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China Author Yu, Hong 0000-0002-2149-5714 Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan, China text MycoKeys 2024 2024-07-03 106 287 301 journal article 299502 10.3897/mycokeys.106.117205 f052f8f5-a82a-4833-a12a-3e22c420cf62 Amphichorda felina (DC.) Fr., Syst. orb. veg. (Lundae) 1: 170 (1825). Fig. 3 Description. The morphological description of this study is based on the specimen, CBS 250.34. Sexual morph : Undetermined. Asexual morph : Colonies on PDA attaining a diameter of 36–38 mm after a month at 25 ° C, white to creamy-white, hard texture, felt-like, reverse black-brown, many conidia assemble to form powder. Hyphae branched, smooth-walled, septate, hyaline, 1.2–2.4 µm wide. Phialides arising laterally from aerial hyphae, erect or irregularly curved, 1.5–4.1 × 1.8–2.9 µm. Conidia 2.9–4.7 × 2.4–3.5 µm, one-celled, smooth-walled, hyaline, broadly ellipsoid or subglobose, single or aggregated into spheres. Chlamydospores not observed. Figure 3. Morphology of Amphichorda felina A, B colony character on PDA medium after 30 d ( A obverse B reverse) C – K conidiophores, conidiogenous cells and conidia. Scale bars: 2 cm ( A, B ); 10 µm ( C – F, I, K ); 5 µm ( G – H, J ). Substrate. Pupa of Anaitis efformata , rabbit dung, mouldy leaves, porcupine dung, cat dung. Distribution. Argentina , Britain, France , Germany . Commentary. Guerra-Mateo et al. (2023) proposed that the strain CBS 250.34 can be accepted as a reference to stabilise the nomenclature of Amphichorda felina and thus the genus Amphichorda , but should be avoided to indicate it as a type strain. In this study, the strain, CBS 250.34, was available in the CBS culture collection and morphological observations were made. Its morphology was generally consistent with those described by De Hoog (1972) , with one difference being that this study extended the phialides (1.5–8.5 × 1.8–2.9 µm) and conidia size range of this species (2.5–4.7 × 2–3.5 µm).