A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles andywarreni Arias-Penna, sp. nov. Figs 20 , 21 Female. Body length 2.88 mm , antenna length 3.03 mm , fore wing length 3.13 mm . Type material. Holotype : ECUADOR • 1♀ ; EC-26009, YY-A051; Napo , Yanayacu Biological Station , Sendero Macuculoma , Plot 358; cloud forest; 2,091 m ; - 0.6, -77.883333; 07.ix.2007 ; Rafael Granizo leg.; caterpillar collected in fourth instar; loose groups of brown cocoons formed on 18.ix.2007 ; adult parasitoids emerged on 05.x.2007 ; ( PUCE ) . Paratypes . • 17 ( 4♀ , 1♂ ) ( 12♀ , 0 ♂ ); EC-26009, YY-A051; same data as for holotype; ( PUCE ) . Diagnosis. Distal antennal flagellomere longer than penultimate, posterior ocelar line shorter than ocular ocelar line, mesoscutum punctation distinct throughout ( Figs 20E , 21B ), lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2, edges of median area on T2 polished and followed by a deep groove ( Figs 20G , 21D ), axillary trough of metanotum proximally with semircular/undulate carina, distally smooth ( Figs 20F , 21C ), precoxal groove shallow, but visible ( Figs 20I , 21E ), anteroventral contour of mesopleuron straight/angulate or nearly so ( Figs 20I , 21E ), and fore wing with r vein curved, outer side of junction of r and 2RS veins forming a distinct stub ( Fig. 20K ). Figure 20. Glyptapanteles andywarreni sp. nov. female EC-26009 YY-A051 A Habitus B Head, frontal view C Head, pronotum, propleuron, lateral view D Genitalia: hypopygium, ovipositor, ovipositor sheaths, lateral view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T1-2, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view K, L Wings K Fore L Hind. Figure 21. Glyptapanteles andywarreni sp. nov. male EC-26009 YY-A051 A Habitus B Mesonotum, dorsal view C Scutellum, metanotum, propodeum, dorsal view D, F Metasoma D Dorsal view F Lateral view E Mesosoma, lateral view. Coloration ( Fig. 20 A-L ). General body coloration brown-black except clypeus and mandibles with yellow-brown coloration; glossa, maxillary and labial palps, and tegulae yellow; both dorsal and ventral furrows of pronotum, ventral edge of mesopleuron, epicnemial ridge, and lunules with reddish brown tints. Eyes silver and ocelli yellowish. Fore and middle legs yellow except coxae brown-black and claws brown; hind legs yellow except coxae black, 1/4 distal of femora, 3/4 proximal of tibia and tarsomeres brown. Petiole on T1 black, contours darkened and sublateral areas yellow; T2 with median area dark brown, contours darkened, wide adjacent area light yellow-brown, and lateral ends yellow; T3 yellow, but medially with an inverted triangle yellow-brown, proximal edges of inverted triangle area coincides with the width of median plus adjacent areas on T2; T4 yellow-brown/light brown with proximal corners yellow; T5 and beyond completely dark brown; distally each tergum with a narrow whitish/yellowish transparent band. In lateral view, T1-3 completely yellow; T4 and beyond dorsally brown and ventrally yellow, extent of brown area increasing from proximal to distal. S1-4 completely yellow; penultimate sternum yellow, ventrally with a brown spot; hypopygium completely brown. Description. Head ( Fig. 20 A-C ). Head triangular with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.25:0.07, 0.25:0.07, 0.25:0.07), distal antennal flagellomere longer than penultimate (0.14:0.07, 0.10:0.07), antenna longer than body (3.03, 2.88); antennal scrobes-frons shallow. Face convex with dense fine punctations, interspaces smooth, and longitudinal median carina present. Frons punctate. Temple wide, punctate and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.14). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally rounded and dorsally wide. Mesosoma ( Fig. 20E, F, I ). Mesosoma dorsoventrally convex. Mesoscutum with narrow grooves/dents taking the place of notauli, punctation distinct throughout, and interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS , scutellar punctation distinct throughout, in profile scutellum slightly convex, but on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM ; ATS demilune with a little and incomplete parallel carinae only proximally; dorsal ATS groove smooth. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation with a smooth and shiny sloped transverse strip. Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM ; PFM thick and smooth; ATM proximally with semircular/undulate carina and distally smooth. Propodeum without median longitudinal carina, proximal half weakly curved with medium-sized sculpture and distal half with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum all smooth with a distinct dorsal furrow, dorsally with a well-defined smooth band. Propleuron with fine punctations throughout and dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge convex, teardrop-shaped. Legs ( Fig. 20A ). Ventral margin of fore telotarsus entire, but with a tiny curved seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.15, 0.07). Hind coxa with punctation only on ventral surface, dorsal outer depression present, inner spur of hind tibia much longer than outer spur (0.25, 0.17), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.16, 0.13). Wings ( Fig. 20K, L ). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a distinct stub; 2M vein straight or slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, complete, but junction with 1-1A vein spectral. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma ( Fig. 20D, G, H, J ). Metasoma laterally compressed. Petiole on T1 completely smooth and polished, with faint, satin-like sheen, petiole evenly narrowing distally (length 0.40, maximum width 0.20, minimum width 0.10) and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.18, length T2 0.18), edges of median area polished and lateral grooves deep, median area broader than long (length 0.18, maximum width 0.30, minimum width 0.08); T2 with scattered pubescence only distally. T3 longer than T2 (0.27, 0.18) and with scattered pubescence only distally. Pubescence on hypopygium dense. Cocoons. Light brown oval cocoons with messy/disordered/fluffy silk fibers. Comments. Distally the pronotum at different level than mesopleuron and forming a deep hollow. The lateral margins of the median area on T2 are delicately curved (concave, Figs 20G, H , 21D ) resembling the median area on T2 of G. bourquini (Blanchard) and G. ecuadorius ( Whitfield et al. 2002a , Figs 2 , 14 ). Male ( Fig. 21 A-F ). Coloration similar to females but darkened. Dorsally, T3 brown with lateral ends yellow-brown rather than yellow and with a brown inverted-triangle area. Etymology. Andrew (Andy) D. Warren is an American lepidopterist, specialized on Hesperiidae . He is working as Senior Collections Manager at McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida , Gainesville, FL , USA . Distribution. Parasitized caterpillar was collected in Ecuador , Napo , Yanayacu Biological Station (Sendero Macuculoma), during September 2007 at 2,091 m in cloud forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Undetermined species of Noctuidae feeding on Evodianthus funifer ( Cyclanthaceae ). Caterpillar was collected in fourth instar.