Eimeria spp. (Apicomplexa: Eimeriidae) in Black Caiman Melanosuchus niger (Crocodilia: Alligatoridae) from the Amazon Region, Brazil, with a Description of Two New Coccidian Species Author Viana, Lúcio André Author Motta, Adarene Guimarães da Silva Author Coutinho, Marcos Eduardo Author Rocha, Carlos Frederico Duarte text Acta Protozoologica 2013 52 4 283 289 https://www.mendeley.com/catalogue/fedb20b7-9daf-3e50-8f1a-730d18bb9385/ journal article 10.4467/16890027AP.13.025.1317 1689-0027 13193295 Eimeria nigeri n. sp. ( Figs 1–2 ) Description of sporulated oocysts: Oocysts spheroidal to subspheroidal, wall composed of 2 layers (n = 49), ~1.2 (1.0–1.3) thick; outer is slightly sculptured, colorless, ~ 2/3 of total thickness, while inner appears as a dark line; sporulated oocysts (n = 50) 29.2 × 28.1 (23– 32 × 23–30), L/W ratio 1.0 (1.0–1.1); M, PG: both absent; OR: mostly spheroidal globule, 12.1 × 11.0 (9.3–14 × 8–13), composed of at least 3 different globules, each inside the next larger body. Distinctive feature of oocyst: the complex OR composed of multiple bodies. Description of sporocysts and sporozoites: Sporocysts ovoidal, slightly pointed at one end, (n = 48) 15.5 × 9.7 (12–17 × 8–11), L/W ratio 1.6 (1.2–2.0); SB: prominent, 2.2 × 1.7 (1–3 × 1–2); SSB, PSB: both absent; SR: condensed group of large globules that appear to be enclosed by a dense matrix of tiny granules. Distinctive feature of sporocyst: SR of large globules that may be membrane bound. Sporozoite with 2 large refractile bodies spheroid and located in the middle and posterior end. Table 1. The prevalence of simple and mixed infections of Eimeria spp. sampled from 23 black caimans from the Reserva Extrativista Lago Cuniã (RESEX Cuniã), within the Brazilian state of Rondonia.

Infection	Species	N	Prevalence (%)
Simple	E. paraguayensis	3	16
E. nigeri	1	5
E. portovelhensis	0	0
Total	4	21
Mixed	E. paraguayensis + E. nigeri	11	58
E. paraguayensis + E. nigeri + Eimeria portovelhensis	3	16
E. nigeri + Eimeria portovelhensis	1	5
Total	15	79

Type-host: The black caiman Melanosuchus niger Spix, 1825 ( Crocodylia , Alligatoridae ). Type-locality: Extractive Reserve Lake Cuniã ( 08°19′05.0″S and 63°29′48.9″W ), Porto Velho County , Rondonia State , Brazil . Site of infection: Not investigated. Sporulation: Unknown. Oocyst suspensions were kept in vials of 2.5% K 2 Cr 2 O 7 solution for two weeks prior to examination. Prevalence: 16/23 (70%). Etymology: The specific name is designated by specific name of the host. Type-specimens: One-half of the oocysts were kept in 10% aqueous buffered formalin (v/v) and the other half in 70% ethanol, according to Duszynski and Gardner (1991) . Both samples were deposited in the Parasite Collection at the Department of Animal Parasitology , which is located at the Universidade Federal Rural do Rio de Janeiro , within the municipality of Seropédica, Rio de Janeiro , Brazil . Phototypes and line drawings were deposited as well, under repository number P-43/2012 .